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선형 GPA 기법을 이용한 터보프롭엔진의 성능 측정변수 최적화 연구
공창덕,기자영,신현기 朝鮮大學校 機械技術硏究所 2000 機械技術硏究 Vol.3 No.1
소·중형 산업용 항공기나 초등 훈련기용으로 많이 이용되고 있는 터보프롭 엔진의 정상 상태 성능해석 프로그램을 개발하고 성능진단을 위해 선형 GPA 기법을 적용하였다. 정상상태 성능해석 프로그램의 검증을 위해 상용 정상상태 해석 프로그램인 TURBOMARCH 해석결과와 비교하였다. 지상정지조건에서의 성능 및 비행마하수에 따른 출력 등을 비교한 결과 각 구성품의 입, 출구 온도 및 압력, 출력등에서는 약 3%이하의 오차율을 보였으며, 마하수 변화에 따른 출력 비교에서도 최대오차율 2.4% 이내로 프로그램의 신뢰성을 확인하였고 고도와 비행마하수에 따른 성능해석을 수행하였다. 선형 GPA 기법의 계측변수의 선정에 따른 정확성을 알기위해 종속변수의 선정을 다르게 하여 오차율을 알아보았다. 성능저하 원인으로는 압축기에만 오염이 발생하였을 경우, 압축기와 압축기 터빈에 각각 오염과 부식이 발생하였을 경우, 압축기 터빈과 동력터빈에 동시에 부식이 발생하였을 경우, 압축기, 압축기 터빈, 동력터빈이 모두 오염과 부식이 발생하였을 경우를 가정하였다. 해석결과 계측기 변수가 많을수록 RMS 오차가 적었으며 같은 수의 계측기 변수라 하더라도 어떠한 변수를 계측하는가에 따라 오차율이 달라짐을 알 수 있었다. 비교적 오차율이 적으면서 경제성이 있는 경우는 8개의 측정변수를 이용한 경우로 최적의 계측기 수 및 계측기 변수 선정이 중요함을 알 수 있었다. The steady-state performance analysis program for turboprop engine which was used for a small, middle industrial aircraft and a basic trainer aircraft was developed and linear Gas Path Analysis method was applied to Engine Health Monitoring for Turboprop engine. This program was compared with TURBOMARCH program which is well known with performance and power according to flight Mach No. at the standard atmospheric condition to prove a steady-state performance analysis program. From the result, inlet, exit temperature and pressure of each component had error within 3% and especially power according to flight Mach No. had error within 2.4% so that this program could be assured. To make sure if linear Gas Path Analysis is reasonable four cases were selected. The first is the case that fouling is occurred in compressor only. The second is the case that fouling is occurred in compressor and erosion is occurred in turbine. The third is the case that erosion is occurred in both compressor and turbine and power turbine at the same time. Finally, the case that fouling and erosion are occurred in compressor, compressor turbine and power turbine was selected. Different parameters were selected impartially among the independent parameters so that the effect of measurement parameter selection was observed. From the result, the more measurement parameters the smaller RMS error and even though the number of measurement parameters was the same, the RMS error was obtained differently according to which measurement parameters were selected. The case using eight instrument parameters of case Ⅳ-4 had small error comparably and was economic and it was important to select optimal number of measurement and optimal measurement parameters.
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Hyun Gi Kong(Hyun Gi Kong),Mee Kyung Sang(Mee Kyung Sang),Ju Hee An(Ju Hee An),Songhwa Kim(Songhwa Kim),Yong Ju Jin(Yong Ju Jin),Jaekyeong Song(Jaekyeong Song) 한국식물병리학회 2022 Plant Pathology Journal Vol.38 No.6
Bacterial wilt caused by Ralstonia solanacearum is considered one of the most harmful diseases of pepper plants. Recently, research on plant disease control through the rhizosphere microbiome has been actively conducted. In this study, the relationship with disease occurrence between the neighboring plant confirmed by analyzing the physicochemical properties of the rhizosphere soil and changes in the microbial community. The results confirmed that the microbial community changes significantly depending on the organic matters, P2O5, and clay in the soil. Despite significant differences in microbial communities according to soil composition, Actinobacteriota at the phylum level was higher in healthy plant rhizosphere (mean of relative abundance, D: 8.05 ± 1.13; H: 10.06 ± 1.59). These results suggest that Actinobacteriota may be associated with bacterial wilt disease. In this study, we present basic information for constructing of healthy soil in the future by presenting the major microbial groups that can suppress bacterial wilt.
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Kong, Hyun Gi,Kim, Nam Hee,Lee, Seung Yeup,Lee, Seon-Woo The Korean Society of Plant Pathology 2016 Plant Pathology Journal Vol.32 No.2
Pseudomonas fluorescens pc78 is an effective biocontrol agent for soil-borne fungal diseases. We previously constructed a P43-gfp tagged biocontrol bacteria P. fluorescens pc78-48 to investigate bacterial traits in natural ecosystem and the environmental risk of genetically modified biocontrol bacteria in tomato rhizosphere. Fluctuation of culturable bacteria profile, microbial community structure, and potential horizontal gene transfer was investigated over time after the bacteria treatment to the tomato rhizosphere. Tagged gene transfer to other organisms such as tomato plants and bacteria cultured on various media was examined by polymerase chain reaction, using gene specific primers. Transfer of chromosomally integrated P43-gfp from pc78 to other organisms was not apparent. Population and colony types of culturable bacteria were not significantly affected by the introduction of P. fluorescens pc78 or pc78-48 into tomato rhizosphere. Additionally, terminal restriction fragment length polymorphism profiles were investigated to estimate the influence on the microbial community structure in tomato rhizosphere between non-treated and pc78-48-treated samples. Interestingly, rhizosphere soil treated with strain pc78-48 exhibited a significantly different bacterial community structure compared to that of non-treated rhizosphere soil. Our results suggest that biocontrol bacteria treatment influences microbial community in tomato rhizosphere, while the chromosomally modified biocontrol bacteria may not pose any specific environmental risk in terms of gene transfer.
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Kong, Hyun-Gi,Choi, Ki-Hyuck,Heo, Kwang-Ryool,Lee, Kwang-Youll,Lee, Hyoung-Ju,Moon, Byung-Ju,Lee, Seon-Woo The Korean Society of Plant Pathology 2009 Plant Pathology Journal Vol.25 No.2
Marking biocontrol bacteria is an essential step to monitor bacterial behavior in natural environments before application in agricultural ecosystem. In this study, we presented the simple green fluorescent protein (GFP) reporter system driven by the promoter active in Bacillus species for tagging of the biocontrol bacteria. A constitutive promoter P43 from Bacillus subtilis was fused to an enhanced promoterless gfp gene by overlap extension PCR. The GFP expression was demonstrated by the high fluorescence intensity detected in B. subtilis and Escherichia coli transformed with the P43-gfp fusion construct, respectively. The GFP reporter system was further investigated in two bacterial biocontrol strains B. licheniformis and Pseudomonas fluorescens. When the reconstructed plasmid pWH34G was introduced into B. licheniformis, GFP level measured with the fluorescence intensity in B. licheniformis was almost equivalent to that in B. subtilis. However, GFP expression level was extremely low in other biocontrol bacteria P. fluorescens by transposon based stable insertion of the P43-gfp construct into the bacterial chromosome. This study provides information regarding to the efficient biomarker P43-gfp fusion construct for bio-control Bacillus species.
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Hyun Gi Kong,Hyeonheui Ham,Mi-Hyun Lee,Dong Suk Park,Yong Hwan Lee 한국식물병리학회 2021 Plant Pathology Journal Vol.37 No.4
Despite the plant microbiota plays an important role in plant health, little is known about the potential interactions of the flower microbiota with pathogens. In this study, we investigated the microbial community of apple blossoms when infected with Erwinia amylovora. The long-read sequencing technology, which significantly increased the genome sequence resolution, thus enabling the characterization of fire blight- induced changes in the flower microbial community. Each sample showed a unique microbial community at the species level. Pantoea agglomerans and P. allii were the most predominant bacteria in healthy flowers, whereas E. amylovora comprised more than 90% of the microbial population in diseased flowers. Furthermore, gene function analysis revealed that glucose and xylose metabolism were enriched in diseased flowers. Overall, our results showed that the microbiome of apple blossoms is rich in specific bacteria, and the nutritional composition of flowers is important for the incidence and spread of bacterial disease.
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Kong, Hyun-Gi,Lee, Hyoung-Ju,Bae, Ju-Young,Kim, Nam-Hee,Moon, Byung-Ju,Lee, Seon-Woo The Korean Society of Plant Pathology 2010 Plant Pathology Journal Vol.26 No.3
Spatial and temporal distribution of Bacillus licheniformis N1 was investigated over time on the leaves, petioles and crowns of the strawberry plants. Bacterial population on the strawberry plants was quantified over time by selective plating. Bacterial population of N1 containing a plasmid pWH43G carrying green fluorescent protein (GFP) declined relatively faster on the plant surface as compared to the Strain N1 itself. However, this result was found to be enough to utilize the strain to visualize bacterial colonization on the plant surface. When B. licheniformis N1 was treated together with Silwet L-77 at 0.03%, the bacterial population on plant surface persisted for up to 7 days. B. licheniformis N1 (pWH43G) containing Silwet L-77 was applied on the strawberry plants and the GFP expressing bacteria were visualized by confocal laser scanning microscopy. Bacterial persistence was also investigated in a growth chamber and in a plastic house after N1 bioformulation treatment on the strawberry plant. The Strain N1 colonized three different tissues well and persisted over 3 to 5 days on the strawberry plants. They formed bacterial aggregates on plant surfaces for at least 3 days, resulting in a biofilm to resist fluctuating plant surface environment. However, the bacterial persistence dramatically declined after 7 days in all tested tissues in a plastic house. This study suggest that B. licheniformis N1 colonizes the strawberry plant surface and persists for a long time in a controlled growth chamber, while it can not persist over 7 days on the plant surface in a plastic house.
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Kong, Hyun-Gi,Kim, Jin-Cheol,Choi, Gyoung-Ja,Lee, Kwang-Youll,Kim, Hyun-Ju,Hwang, Eul-Chul,Moon, Byung-Ju,Lee, Seon-Woo The Korean Society of Plant Pathology 2010 Plant Pathology Journal Vol.26 No.2
Bacillus licheniformis N1, previously developed as a biofungicide formulation N1E to control gray mold disease of plants, was investigated to study the bacterial traits that may be involved in its biological control activity. Two N1E based formulations, bacterial cell based formulation PN1E and culture supernatant based formulation SN1E, were evaluated for disease control activity against gray mold disease of tomato and strawberry plants. Neither PN1E nor SN1E was as effective as the original formulation N1E. Fractionation of antifungal compounds from the bacterial culture supernatant of B. licheniformis N1 indicated that two different cyclic lipopeptides were responsible for the antimicrobial activity of the N1 strain. These two purified compounds were identified as iturin A and surfactin by HPLC and LCMS. The purified lipopeptides were evaluated for plant disease control activity against seven plant diseases. Crude extracts and purified compounds applied at 500 ${\mu}g/ml$ concentration controlled tomato gray mold, tomato late blight and pepper anthracnose effectively with over 70% disease control value. While iturin showed broad spectrum activity against all tested plant diseases, the control activity by surfactin was limited to tomato gray mold, tomato late blight, and pepper anthracnose. Although antifungal compounds from B. licheniformis N1 exhibited disease control activity, our results suggested that bacterial cells present in the N1E formulation also contribute to the disease control activity together with the antifungal compounds.
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