Effect of ER signal peptides (KDEL) on expression and function of monoclonal antibody in plant

Da-Young Park,Eun-Yi Oh,Zhe Lu,Yangkang So,Mi-Hyun Ahn,Mira Song,Kyung-Jin Lee,Doo-Byoung Oh,Youngkwan Kim,Kisung Ko 한국당과학회 2009 한국당과학회 학술대회 Vol.2009 No.1

Plant genetic engineering led to the production of plant-derived mAb (mAbP), which provides a safe and economically feasible alternative to the current animal expression systems. In this study, the expression level of mAbP SO57 between with and without Lys-Asp-Glu-Leu (KDEL) was compared in transgenic plant. PCR and PT-PCR analyse showed stable gene insertion transcription of heavy and light chain genes of mAbP SO57 with or without KDEL in plant, respectively. We validated expression of mAbP SO57 by western blot. Western blot showed the significantly higher expression level of mAbP SO57 with KDEL compared to without KDEL. Flow cytometry (FACS) analysis showed that the Fc domains of both purified mAbP and mammalian-derived mAb (mAbM) evidenced similar binding activity to the FcγRI receptor (CD64). High performance liquid chromatography (HPLC) analysed, glycosylation patterns of mAbP SO57 with or without KDEL. The mAbP SO57 with KDEL had glycan profile with both oligomannose type (47.6%) and golgi type (52.4%), while the mAbP SO57 without KDEL had only golgi type (100%) glycans. Neutralizing analysis with rabies virus CVS-11 showed the similar neutralizing activity between mAbP SO57 with and without KDEL. These results suggest the potential of mAbP SO57 for rabies immunotherapy, regardless of plant specific glycan structures.

가스상 대기오염물질에 의한 종이 기록물의 가속열화 특성 연구

정소윤(So-Yoon Jeong),전수연(Soo-Yeon Jeon),백소라(So-Ra Baek),정현석(Hyun-Seok Jeong),이진희(Jin-Hee Lee),김형진(Hyoung-Jin Kim) 한국펄프·종이공학회 2015 펄프.종이기술 Vol.47 No.4

Paper records were generally degraded by some factors from atmospheric environments, like temperature, relative humidity or air pollutants. In this study, the degradation behavior of paper records by single or mixed gases of NO₂, SO₂, HCHO and TVOC was evaluated. The mechanical, optical and chemical properties of 4 kinds of paper (acid and neutral-based printing paper, traditional Hanji, and filter paper) were directly and indirectly affected by gaseous harmful materials. The brightness and L<SUP>*</SUP> value in all papers were slightly increased by accelerated aging under gaseous HCHO and TVOCs, but highly decreased by conditions under gases NO₂ and SO₂. The optical properties of paper records were most vulnerable in acid-based paper and high stable in filter paper and traditional Hanji by air pollutant degradation. The aging treatments under mixed gas pollutants including NO₂ resulted in decrease of physical, mechanical and optical properties of paper, so it was supposed that the concentration of NO₂ gas would be strictly controlled for optimum indoor air quality management in domestic storage centers for paper records.

또 하나의 냉전

박소현(Park So-Hyun) 현대미술사학회 2006 현대미술사연구 Vol.19 No.-

In 1970, the so-called 'revisionists' of the United States criticised that 'the triumph of American art' was the product of 'cultural Cold War' and Abstract Expressionism as well as the Museum of Modern Art in New York were the weapons of Cold War. Their criticism suggested the problematic issues about modern art in Cold War, but they had some logical limits in their literatures: on the one hand, they were almost interested in the centre of modern art, Paris or New York, and on the other hand, they believed the 'centre', understood as American art in New York after World War Ⅱ, was getting to be diffused into the other areas step by step, for example, Europe in the 1950s, Asia in the 1960s. This means even the critical 'revisionists', probably unconsciously, were not free from and consequently reproduced the geopolitics of the 'centre' with regard to modern art. As for Japan, however, the cultural Cold War' did not come late in the 1960s, but started from the Occupation of the GHQ (General Headquarters/Supreme Commander for Allied Powers) in 1945. And although under the Occupation, the meaning of modern art in Japan was not directly connected to 'the triumph of American art', rather the exhibitions of French modern art were vigorously held. These exhibitions were taken place almost by the newborn 'Kokuritsuhakubutsukan (National Museum)' which had been the property of Tenno (Emperor of Japan) but was reconstituted as that of people by the GHQ on the basis of the Occupational ideology of 'democracy' after the defeat of war. In short, there appeared the 'cultural Cold War' in Japan during the Occupation years in the form of 'democratic modern' French art. And more, the ideology of 'democracy' was so discussive, inconsistent and changing one that could not say that the ever-determined principle of the 'centre' was received as it was without any resistance or modification in Japan. Therefore, I think the case of Japan under the Occupation is a good one to question about the geopolitics of 'centre' reproduced by the 'revisionists'. During the Occupation years, the meaning of 'democracy' and 'democratic modern' art was changed gradually, from anti-militarism (or anti-nationalism) to anti-communism (or anti-socialism) and ended up to the resurrection of Japanese nationalism. With these changes, the French artists or artistic movements was selectively accentuated, from Impressionists to Fauvists or Cubists and to decorative Fauvists, especially Matisse. First, right after the defeat of war, because the Occupational principle was centered on the anti-militarism, 'democracy' was interpreted as a broad one, including socialism, and so there happened a series of competitions for which one is more 'democratic' art between Impressionist realism and socialist realism. But with the opening of 'Kokuritsuhakubutsukan' in 1947, in the second place, the meaning of 'democracy' in that institution was changed into anti-communism, and at the same time 'the autonomy of art(art for art's sake)' was underlined as the institutional principle. On the behalf of this principle, art criticisms and artistic discourses also put it that Fauvists or Cubists was more developed ones than Impressionists because the first was closer to 'the autonomy of art' than the last. This interpretation was conscious of the formulation of modern art by Museum of Modern Art in New York regarded as 'center of art world' and the extraordinary Museum of Modern Art was open within the Kokuritsuhakubutsukan in 1948. And then, from 1949 to 1951, the third aspect of these changes, The GHQ decided to make Japan anti-communist base in Asia, and for the purpose of it to make Japan an independent nation-state was needed. So the Kokuritsuhakubutsukan made a great point of worldliness of Japanese art, and emphasized the superiority of Japanese classics or traditional culture which had been regarded to influence French modern arts. This was the return of Japonism which ha

Effect of ER signal peptides (KDEL) on expression and function of anti-virus monoclonal antibody in plant

Da-Young Park,Yangkang So,Kyung-Jin Lee,Zhe Lu,Eun-Yi Oh,Kwang-Wok Min,Seul-Ki Lee,Jung-Hwan Lee,Mi-Hyun Ahn,Mira Song,Doo-Byoung Oh,Youngkwan Kim,Kisung Ko 한국당과학회 2009 한국당과학회 학술대회 Vol.2009 No.1

Plant genetic engineering has led to the production of plant-derived mAb (mAbP), which provides a safe and economically feasible alternative to the current antibody expression systems. In this study, the expression levels of mAbP SO57 with or without ER-signal peptides (Lys-Asp-Gly-Leu;KDEL) in transgenic tobacco plants were analysed in transgenic plant. PCR and Reverse Transcription-PCR analyses showed existence of heavy and light chain genes of mAb with or without KEDL and their transcription in plant, respectively. Western blot showed that the expression levels of mAbP SO57 with KDEL were significantly higher than that without KDEL. Flow cytometry analysis showed that the Fc domains of both purified mAbP and mammalian-derived mAb have similar binding activity to the FcγRI receptor. High-performance liquid chromatography analysis showed that the mAbP SO57 with KDEL had glycan profile with both oligomannose and golgi type, whereas the mAbP SO57 without KDEL had only golgi type glycans. Neutralizing analysis with rabies virus CVS-11 showed the similar neutralizing activity between mAbP SO57 with and without KDEL. These results suggest that the potential of mAbP SO57 for rabies immunotherapy is regardless of plant specific glycan structures.

서산지역의 대기질 경년변화 특성에 관한 연구

손병현(Shon Byung-Hyun),박동소(Park, Tong-So) 한국산학기술학회 2008 한국산학기술학회논문지 Vol.9 No.3

본 연구에서는 1998년부터 2006년까지 9년 동안의 서산 지역 대기오염물질 농도 변화 특성을 조사하였다. 조사 결과, 최근 3년 동안 CO와 O₃을 제외한 SO₂, NO₂ 및 PM-10은 농도 변화가 거의 없는 안정화 단계를 보였다. NO₂, CO 및 SO₂의 월평균농도 변화는 여름철에 감소하다 겨울철에 증가하는 경향을 보였으며 O₃과 PM-10은 봄철에 높은 농도를 보이며, 강수가 집중되는 여름철에 가장 낮은 농도분포를 보였다. 2006년 12월까지 오존의 1시간 기준치인 0.1 ppm을 초과한 횟수는 동문동이 68회, 독꽂리 93회였고 8시간 기준치얀 0.06 ppm을 초과한 횟수는 동문동 210 회, 독곶리 213회였다. 또한 미세먼지의 경우 24시간기준인 150 ㎍/㎥을 초과한 횟수가 동문동 25회, 독곶리 54회로 나타났다. This study was performed to evaluate the characteristics of current air quality and to examine the monthly and yearly variation of SO₂, O₃, CO, NO₂, and fine particulate matter(PM-10) concentration in Seosan for the period of 1998-2006. During the recent 3 years, from 2004 to 2006, the yearly mean concentration of SO₂, NO₂, and PM-10 remained stability but CO and O₃ are gradually decreased. The monthly mean concentration of NO₂, CO, and SO₂ were high in the winter season and decreased steadily to the summer season, but the maximum concentration of O₃ and PM-10 appeared in the springtime. The number of days exceeding 24-hour standards for PM-10 were 54 times at Dokgodri and 25 times at Dongmundong, The number of sampling days exceeding the l-hour and 8-hour standards for O₃ were 68 and 210 times at Dongrmundong and at Dokgotri 93 and 213 times, respectively.

바우하우스의 기계인간

박소현(So-Hyun Park) 현대미술사학회 2003 현대미술사연구 Vol.15 No.-

This thesis started from the question of the exclusive boundary of Modernist Art. Modernism has been established on the basis of the opposites such as eyes and body, the spiritual and the material, art and non-art, avant-garde and kitsch, original and reproduction, maleness and femaleness, nature and technology, and then, arming with historical necessity, destined as unreversible law, universal moral, and fundamental essence through American theorists such like Alfred H. Barr,Jr., Clement Greenberg, and Michael Fried. It became a very meaningful turning point that in the sixties, the myth of Modernism was criticized and lost its validity, and reappreciated just like a historical happening by the stream of post-Modernism. But it leaves room for overlooking the discordance between American Modernist theories and historical modernists that we consider as though the myth of Modernism was destroyed just by the criticism and reflection of ex-post-facto. So this study focused on the fact that in the realm of Modernism there have been multiple discourses, which could not be unified as only one narrative, and also a lot of contradictions and ruptures threatening the purity of Modernism. This thesis selected two historical facts among them. One is that 'robot avant-garde', which overwhelmed modern art in the early 20th century, was excluded as marginal stream in the American Modernist theories. Robot avant-garde ardently worshiped mechanical man as a new subject and object of modern art, and as a 'New Man' of the new world of future. Therefore mechanical man was a symbol in which all the ideals of historical avant-gardes and Modernists were condensed. But the mechanical man, by taking both aspects of the hierarchical opposites premised in Modernist discourse, made the distinction between the pure and the impure invalid, and by revealing the points which that opposites were implicated with political, social, and cultural contexts, brought about the doubt of the purity and autonomy of Modernism itself. This doubleness of mechanical man made his existence invisible or marginal in the American Modernist theories. The other is a specific group which has been called as the Bauhaus. The experimentations of the Bauhaus were inclined to so-called 'unified art', and therefore expanded boundary of art included even daily life. To theorists who intended to give Modernism a rigid boundary, therefore, the existence of the Bauhaus was very troublesome. This troublesomeness was well expressed in the monumental lineage of modern art created by A. H. Barr, Jr.. The Bauhaus, which existed as a only cut-off in Barr's table, is a Modernist art and at the same time a non-Modernist art. It is boundary or joint itself between the visible lineage of art and non-artistic contexts existing as an empty blank in that table. For the project of the Bauhaus is the extension of art, with violating the boundary of art, and based on the autonomy of vision, with revealing mechanical body which that vision results from. Therefore, by re-locating robot avant-garde and the Bauhaus into the limited Modernist theories, this thesis intended to relativatize American Modernist discourse and to uncover the fact that Modernism itself was, having countless seams, heterogeneous and non-uniform tendency. The mechanical man of the Bauhaus was constructed through contemporary socio-political contexts and mass culture reversing the purity of Modernism, and the pure vision, accentuated in the field of Modernism, entered upon the stage with and as a part of mechanical body. Consequently, paradoxical aspects such like the mechanical man of the Bauhaus is not the others of Modernist art but one of various centers which made Modernism meaningful, and propose the possibility of multiple Modernism, overcoming singular Modernism which American Modernist theories and the criticism of post-Modernism to Modernism have approved as a premise.

대기 중 유해성분(NO₂, SO₂, HCHO)에 의해 열화된 종이의 물리적 특성

백소라(So Ra Beck),정소윤(So Yoon Jeong),정현석(Hyun Suk Jung),이진희(Jin Hee Lee),김형진(Hyoung Jin Kim),김신도(Shin Do Kim) 한국펄프·종이공학회 2014 한국펄프·종이공학회 학술발표논문집 Vol.2014 No.10

논문 : 북한의 홍보용 웹사이트 시각요소에 대한 연구 -정권에 따른 인터넷활용 정책의 변화를 중심으로

김소현 ( So Hyun Kim ),김현석 ( Hyun Suk Kim ) 디자인융복합학회 2012 디자인융복합연구 Vol.11 No.4

북한은 21세기 초부터 IT 산업을 경제회복의 전략산업으로 강조하였으나 독재정권의 체제유지 전략 때문에 폐쇄적인 정책을 유지했다. 하지만 최근들어 정권교체와 새로운 체제로의 전환, 모바일 산업의 발전과 인터넷활용의 다양한 시도를 통해 국제사회에서 조용한 변화를 예고하고 있다. 이 연구는 북한 정권교체의 시대상황 변화가 인터넷활용 정책과 웹사이트시각요소에 어떠한 변화를 일으켰는지 형식과 내용에 대해 알아보고자 하는 목적이 있다. 연구목적을 위해 선행연구(김소현, 2007) 고찰을 통해 김정일 정권 시의 인터넷정책과 웹사이트 시각요소에 나타난 특성을 도출하였다. 이 특성은 현재의 시각요소 특성과 비교하여 어떤 변화가 있었는지 표현방식과 내용으로 구분하여 분석하였다. 또한, 북한 인터넷 정책이 어떻게 변화하고 있는지를 살펴보기 위해서는 김정은 후계체제 공식화 시기 이후에 개설된 모바일 웹, 외부서비스(SNS)의 활용 형태를 분석하였다. 시각요소 비교분석과 인터넷활용 형태 분석을 통해 도출한 결과는 현재 북한의 시대상황에 비춰보고 그 변화 원인과 앞으로의 발전 방향을 예측해보았다. 이 연구는 시각디자인 분야에서 거의 다루지 않는 북한의 시각디자인 분야를 다양한 측면에서 깊이 있게 연구하였다는 점에서 가치가 있다고 판단된다. 앞으로 이 연구 결과가 남·북한 교류를 위한 웹사이트 구축에 중요한 기본 토대가 되기를 기대한다. The purpose of this study was tried to identify the cause of the changes in the visual elements of North Korea web-sites under the dictatorship and to forecast the future. For the purpose of this study, which is to review the changes in visual elements of North Korea web-sites, web-sites for PR of their country were abstracted from the subjects of the previous study(Kim, So-Hyun, 2007), they were compared with the visual elements of current web-sites in 2012 and the differences between them were analyzed. Additionally, the characteristics of the visual elements in the newly opened web-sites for PR and the mobile webs were analyzed. From the analysis of the visual elements, it was possible to identify the causes of the changes and to forecast the orientation of the changes through the situational review of North Korea at present. It is expected that this study will provide a basic ground for the web-site construction and strategy planning for cultural exchanges between North and South, understanding the political purpose and the characteristics of North Korea.

사업장(事業場) 근로자(勤勞者)의 사상체질(四象體質)에 따른 생활습관(生活習慣), 자각증상(自覺症狀), 건강인식(健康認識)에 관(關)한 연구(硏究)

서명효,강홍구,장두섭,왕명자,차남현,김삼태,권소희,정해경,송용선,이기남,Seo Muoung-Hoy,Kang Hong-Goo,Jahng Doo-Sub,Wang Myoung-Ja,Cha Nam-Hyun,Kim Sam-Tae,Kwon So-Hee,Jung Hae-Kyoung,Song Yung-Sun,Lee Ki-Nam 대한예방한의학회 2003 대한예방한의학회지 Vol.7 No.2

The study was conducted to evaluate the difference of life style, subjective symptoms and health cognition depending on the Sasang Constitution in the industrial workers. The results were as follows ; Constitutional distribution among exposed group were Tae-eum In(38.4%), So-eum In(35.2%), and So-yang In(26.4%) and it was turned out that Tae-eum In took a higher portion of Sasang constitution than other constitutions in technician workers. 'Exercise' and 'Obesity' were found most in Tae-eum In, In So-eum In there were many 'Normal' weight and 'Having digestive disorders' were found most in So-eum In. There were no significant difference in the health cognition among the constitutions. The closer to 'normal' weight, the higher prevalence in 'having' concern about health condition and the more 'normal' condition in sleeping, the higher prevalence of 'having' self-confident in present health.

Impact of the Members' Mood on Information Sharing During Group Discussion Unshared Information, Positive Moods, Negative Moods, Decision-Making

So,Won Hyun 한국시민윤리학회 2009 한국시민윤리학회보 Vol.22 No.2

집단의사결정의 정보추출 모형(information sampling model of group discussion)은 조직 내의 의사결정과정에 필요한 모든 정보를 추출하는데 어려움이 있을 수 있음을, 조직 내의 모든 구성원들이 공유하고 있지 않은 개인의 유일정보(unshared information)의 개념을 이용하여 설명하고 있다. 선행 연구들은 개인들이 집단의사결정의 과정에서 자신들의 유일정보를 언급하기를 꺼려함에 따라, 결국 많은 경우의 집단의사결정에서 최종적으로 선택되는 대처 안은 유일정보가 배제된 공통정보만을 바탕으로 한 것이라는 점을 지적한다. 본 연구에서는 집단구성원 개인의 긍정적 기분이, 집단의사결정 과정에 있어서 원활한 정보교류를 촉진시킨 다는 것을 증명하기 위하여 개인의 기분 요소를 인위적으로 통제 및 조성(control and manipulation)하는데 성공하였으며, 실험적 정보교류과제를 개발하여 조직 내에서 양질의 의사결정을 유도할 수 있음을 입증하였다. 특히 본 연구결과에 의하면 집단구성원 개인이 긍정적 기분을 가지고 있을 때, 그들은 단지 주어진 과제를 형식적으로 마무리하는데 그치지 않고, 과제의 완성도를 높이기 위해서 가능한 모든 정보를 포괄적으로 이용하고자 하는 경향성을 보였다. 본 연구는 실험연구라는 연구방법상의 특성에 의하여 충분한 외적 타당도(external validity)를 확보할 수 없었다는 제한점은 가지고 있으나, 방법론적인 면에 있어서 긍정적 기분과 부정적 기분을 실험적으로 유도함으로써 원활한 정보교류의 촉진과 집단의사결정의 질적 향상을 위한 집단 구성원들의 노력이 부정적 기분의 감소가 아닌 긍정적 기분의 증가에 의해 유도되는 것임을 실증적으로 확인하였다.