Influence of Bundle Diameter and Attachment Point on Kinematic Behavior in Double Bundle Anterior Cruciate Ligament Reconstruction Using Computational Model

Kwon, Oh Soo,Purevsuren, Tserenchimed,Kim, Kyungsoo,Park, Won Man,Kwon, Tae-Kyu,Kim, Yoon Hyuk Hindawi Publishing Corporation 2014 Computational and mathematical methods in medicine Vol.2014 No.-

<P>A protocol to choose the graft diameter attachment point of each bundle has not yet been determined since they are usually dependent on a surgeon's preference. Therefore, the influence of bundle diameters and attachment points on the kinematics of the knee joint needs to be quantitatively analyzed. A three-dimensional knee model was reconstructed with computed tomography images of a 26-year-old man. Based on the model, models of double bundle anterior cruciate ligament (ACL) reconstruction were developed. The anterior tibial translations for the anterior drawer test and the internal tibial rotation for the pivot shift test were investigated according to variation of bundle diameters and attachment points. For the model in this study, the knee kinematics after the double bundle ACL reconstruction were dependent on the attachment point and not much influenced by the bundle diameter although larger sized anterior-medial bundles provided increased stability in the knee joint. Therefore, in the clinical setting, the bundle attachment point needs to be considered prior to the bundle diameter, and the current selection method of graft diameters for both bundles appears justified.</P>

치주질환 심도에 따른 조직내 림프구 및 NK 세포의 변화에 관한 면역조직학적 연구

최호근,이만섭,권영혁 慶熙大學校 齒科大學 1993 慶熙齒大論文集 Vol.15 No.1

티타늄 표면에 대한 배양 골수세포와 치은 섬유아세포의 생체적합성

오충영,박준봉,권영혁,이만섭 慶熙大學校 齒科大學 1995 慶熙齒大論文集 Vol.17 No.2

The purpose of this study was to evaluate the response in aspect of attachment and growth rate of osteablasts and human gingival fibroblasts to the commercially pure titanium(CP titanium) and titanium alloy(Ti-6A1-4V) that are used widely as implant materials, and to obtain the basic information to ideal implant materials. In this study, commercially pure titanium in first test group, titanium alloy(Ti-6A1-4V) in second test group, cobalt-chromemolybdenum alloy(Co-Cr-Mo alloy) in positive control group, and tissue culture polystyrene plate in negative control group were used. The results of this study were as follows. 1. Bone marrow cells cultured on CP titanium and Ti-6A1-4V showed significantly greater attachment and growth rate(p<0.05) compared to Co-Cr-Mo alloy in each time. 2. There were no significant differences(p>0.05) in attachment and growth rate of bone marrow cells cultured on GP titanium and Ti-6A1-4V or tissue culture plate. 3."Most bone marrow cells cultured on CP titanium, Ti-6A1-4V and tissue culture plate were attached well to each substratum in first 2days, and then, grew at higher growth rate. On the other hand, some cells cultured on Co-Cr-Mo alloy failed to attach in first 2 days, and then, attached cells grew at lower growth rate than other groups. 4. Attachment and growth rates of gingival fibroblasts cultured on CP titanium and Ti-6A1-4V showed no significant differences(p>0.05) compared to Co-Cr-Mo alloy in 2 days, but significantly greater increase(p<0.05) in 5 and 9 days. 5. There were no significantly differences(p>0.05) between growth rates on gingival fibroblasts cultured on CP titanium, Ti-6A1-4V and tissue culture plate in 2 and 5 days, but a significant lower growth rate(p<0.05) on CP titanium and Ti-6A.1-4V versus tissue culture plate. 6. Some gingival fibroblasts cultured on all specimen groups failed to attach, but attached cells grew well especially on CP titanium, Ti-6A1-4V and tissue culture plate. 7. There were no significant differences(p>0.05) between growth rates of both bone marrow cells and gingival fibroblasts cultured on CP titanium and Ti-6A1-4V. As a result of this study, both commercially pure titanium and Ti-6AI-4V showed excellent biocompatibility and there was no significant difference in the cellular response to the both metals. Bone marrow cells cultured on each substratum showed significantly greater growth rate and responded sensitively to cytotoxic effects of metal surfaces compared to gingival fibroblasts. Considering cell response to the substrate, it was likely that the composition itself of titanium metals have no significant effects on the biocompatibility. Further study need to be done to evaluate the influence of surface characteristics on cellular responses.

계류기구로 관측한 대구시 야간 안정층 특성에 관한 사례연구

김희종,윤일희,권병혁,허만천 한국환경과학회 2002 한국환경과학회지 Vol.11 No.3

Using measured data at Daegu by tethersonde for the period or 1984~1987, we have investigated the lower atmospheric boundary layer structure including relationships between inversion layer and meteorological factor(wind and temperature), and the inversion strength and inversion height. The inversion layer was defined from the vertical temperature profile and its strength was analyzed with the wind shear as well as the vertical temperature gradient. From October to January, measured inversion layer isn't destroyed, however, in June, after sun rise, it is destroyed by surface heating and mixed layer is developed from surface. According to pasquill stability classes, the moderately stable cases dominated. It's the larger vertical temperature gradient, the lower SBL height. We have introduced B(bulk turbulence scale) which indicated SBL height. It's larger B, the higher SBL height and vice versa. It was noted that the bulk turbulence scale (B) is appropriated to determine the stable boundary layer height.

치주질환 심도에 따른 치아동요도와 교합력의 상관관계에 관한 연구

최조우,이만섭,권영혁 慶熙大學校 齒科大學 1993 慶熙齒大論文集 Vol.15 No.1

The purpose of this study was to investigate the change of tooth mobility and bite force according to periodontal disease severity. Tooth mobility and biting force due to change of viscoelastic property of periodontium were influenced by inflammation of periodontal tissue. 30 patients participated in this study, the periodontal disease severity is evaluated with SBI and attachment loss. SBI and attachment loss were examined by periodontal probe. Tooth mobility was tested two times to each tooth using periotest (Siemens Co, Germany) and bite force was evaluated with MPM-3000 (Nihon Kohden Co, Japan Statistical analysis was applied to correlation (r2) and regression analysis. The obtained results were as follows 1. As the attachment loss increased, tooth mobility increased with significance, and they had highly positive correlation (r-2=0.68) on entire dentition. 2. As the SBI increased, tooth mobility increased with significance, and they had positive correlation (r2=0.37) on entire dentition. 3. As the attachment loss increased, bite force decreased with significance, and they had highly negative correlation (r2=0.42) on maxillary anterior dentition but low negative correlation (r2=0.20) on the other portion of dentition. 4. As the SBI increased, bite force decreased with significance, and they had highly negative correlation (r-2=0.31) on maxillary anterior dentition but low negative correlation (rz=0.16) on the other portion of dentition. 5. As tooth mobility increased, bite force decreased with significance, and they had highly negative correlation (r2=0.32) on maxillary anterior dentition but low negative correlation (r2=0.16) on the other portion of dentition.

치주인대세포와 치은 섬유아세포의 혼합배양이 석회화 결절형성에 미치는 영향

인영미,박준봉,권영혁,이만섭 慶熙大學校 齒科大學 1995 慶熙齒大論文集 Vol.17 No.2

The goal of periodontal therapy is to regenerate the loss of periodontal attachment appratus. Current theories suggest the cells of the periodontium have the capacity, when appropriately triggered, to actively participate in restoring connective tissues, including mineralized tissues. This study was performed to define the hard tissue regeneration effect of periodontal ligament (PDL) cells in nitro and the effect of rate of the composition in gingival fibroblasts (GF) on the hard tissue regeneration capacity of PDI_ cells. For this study, Cell growth rate, alkaline phosphatase(ALPase) levels and the ability to produce mineralized nodules in co-culture of PDL cells and GF were examined. The results were as follows ; 1. At 7 and 15 days, cell growth of co-culture of PDL and GF (50 : 50) was greater than that of PDL cells or GF alone(P<0.05). 2. Measurements of ALPase levels indicated that PDL cells had significantly higher activity when compared with that of co-culture groups and GF only(P<0.05). And, ALPase activity in 10 days was higher than that of 7 days(P<0.05). 3. The tendency of formation of the mineralized nodule were observed dose-depend pattern of PDL cells. There was statistically significant difference among group 1(PDL 100%), 2(PDL 70% GF 30%), and 3(PDL 50% = GF 50%)(P<0.01). But, there was no difference among group 3, 4(PDL 30% : GF 70%), and 5(GF 100%). 4. Also, the number of nodule was greater in co-culture of PDL 70% and GF 30% than in culture of PDL 70%(P<0.05). From the above results, it is assumed that the co-culture of PDL cells and GF stimulates the cell growth, which is not that of PDL cells but GF. And, the activity of ALPase depends on the ratio of PDL cells, and ALPase may relate to the initial phase of nodule formation. Also, it is thought that the calcified nodule formation principally depends on PDL cells, is inhibited by GF, and affected by cell density.

배양골세포 이식이 치조골재생에 미치는 영향

정순준,허익,박준봉,이만섭,권영혁 慶熙大學校 齒科大學 1995 慶熙齒大論文集 Vol.17 No.2

This study was performed to estimate the effects of cultured bone cell inoculated on porous type hydroxyaptite for the regeneration of the artificial alveolar bone defect. In this experiment 3 beagle dogs were used, and each of them were divided into right and left mandible. Every surgical intervention were performed under the general anesthesia by using with intravenous injection of Pentobarbital sodium (30mg/Kg). To reduce the gingival bleeding during surgery, operative site was injected with Lidocaine hydrochloride (1:80,000 Epinephrine) as local anesthesia. After surgery experimental animal were feeded with soft diet(Mighty dog, Frisies Co., U.S.A.) for 1 weeks to avoid irritaion to soft tissue by food. 2 months before surgery both side of mandibular 1st premolar were extracted and bone chips from mandibular body were obtained from all animals. Bone cells were cultured from bone chips obtained from mandible with Dulbecco's Modified Essential Medium contained with 10% Fetal Bovine Serum under the conventional conditions. Porous type hydroxyapatite were immerse into the high concentrated cell suspension solution, and put 4 hours for attachment the cells on the surface of hydroxyapatite. Graft material were inserted on the artificial bone defect after 3 days of culture. Before insertion of cell-inoculated graft material, scanning electronic microscopic observation were performed to confirm the attachment and spreading of cell on the hydroxyapatite surface. 3 artificial bone defects were made with bone trephine drill on Abe both side of mandible of the experimental animal. First defect was designed without insertion of graft material as negative control, second was filled with porous replaniineform hydroxyapatite inoculated with cultured bone marrow cells as expermiental site, and third was filled with graft materials only as positive control. The size of every artificial bone defect was 3 mm in diameter and 3 mm in depth. After the every surgical intervention of animals, oral hygiene program were performed with 0.1% chlorhexidine digluconate. All of the animals were sacrificed at 2, 4, 6 weeks after surgery. For obtaining histological section, tissus were fixed in 2.5% Glutaldehyde and decalcified with Planko-Rycho Solution for 72 hr. Tissue embeding was performed in paraffin and cut parallel to the surface of mandibular body. Section in Bum thickness of tissue was done and stained with Hematoxylin-Eosin. All the specimens were observed under the light microscopy. The following results were obtained. 1. In the case of control site which has no graft material, less inflammatory cell infiltration and rapid new bone forming tendency were revealed compared with experimental groups. But bone surface were observed depression pattern on defect area because of soft tissue invasion into the artificial bone defect during the experimental period. 2. In the porous hydroxyapatite only group, inflammatory cell infiltration was prominet and dense connective tissue were encapsulated around grafted materials. osteoblastic activity in the early stage after surgery was low to compared with grafted with bone cells. 3. In the case of porous hydroxyapatite inoculated with bone cell, less inflammatory cell infiltration and rapid new bone formation activity was revealed than hydroxyapatite only group. Active new bone formation were observed in the early stage of control group. 4. The origin of new bone forming was revealed not from the center of, defected area but from the surface of preexisting bony wall on every specimen. 5. In this experiment, osteoclastic cell was not found around grafted materials, and fibrovascular invasion into regions with no noticeable foreign body reaction. Conclusively, the cultured bone cell inoculated onto the porous hydroxyapatite may have an important role of regeneration of artificial bone defects of alveolar bone.

백서 치아 발거후 잔존 치주인대가 발치와의 치조골 재건에 미치는 영향

조성훈,허익,박준봉,이만섭,권영혁 慶熙大學校 齒科大學 1995 慶熙齒大論文集 Vol.17 No.2

The purpose of this study was to observe the effects of the periodontal ligament on the healing and the formation of alveolar bone in the extraction socket, when this ligament had artificially remained in the socket during the tooth removal. Twenty rats aged 4 weeks were used and devided into the control groups (10) and the experimental groups (10) in this study. The maxillary right and left first molars were extracted in both groups. In the experimental groups the periodontal ligament was remained in the extraction sockets using 0.4% fl-aminopropionitrile, and in the control the periodontal ligament was completely removed by curettage. At 1, 3, 5, 7 and 14 days after the tooth extraction, rats in both groups were serially sacrificed. And the specimens were prepared with HematoxyIin-Eosin stain for the light microscopic evaluation. The results ofthis study were as follows ; 1. On 1 day, the periodontal Iigament was only found in the extraction socket walls of the experimental groups, and there was not the distinguishable difference between the control and the experimental groups. 2. On 3 days, there were more collagen fibers and the appearance of higher cellular density in the experimental groups than in the control. And the cells and collagen of the periodontal ligament were so actively proliferated and synthesized that invaded into the connective tissue of the extraction sockets in the experimental groups. 3. In the experimental groups, the trabecular bone was formed on the basal and lateral bone surface on 5 days. However, there was not the new bone forming appearance in the control groups at this time. 4. On 7 days, the trabecular bone was formed in the control groups. 5. On 14 days, the extraction sockets were almost entirely filled with the bony trabeculae in both groups. But, compared to the control group, the experimental groups showed the prominent differences in the amount & the density of the new bone formed. In conclusion, it was suggested that the residual periodontal ligament tissue in the extraction socket will play a major role as the important cell source in the healing and the new bone formation of the extraction socket.

Poster Session 4 : Tumor Biology Developmental Biology Plant Biology Molecular Medicine and Gene Therapy Prokaryotic Genetics Biotechnology ; Antiproliferative Actitvity of an Aqeous Agrimonia Extract from Leaves of Agrimonia in Human Tumor Cell Lines

( Kwon Hyuk Yun ),( Man Jin Oh ),( Kwang Dong Kim ),( Eun Young Song ),( Young Kyung Choe ),( Kwon Dur Han ) 한국생화학분자생물학회 (구 한국생화학회) 2002 생화학분자생물학회 춘계학술발표논문집 Vol.2002 No.-

Effect of interface-dependent crystalline boundary on sub-threshold characteristics in a solution-processed 6,13-bis(triisopropylsilylethynyl)-pentacene thin-film transistor

Kwon, Jin-Hyuk,Kang, In Man,Bae, Jin-Hyuk EDP Sciences 2014 European physical journal, EPJ. Applied physics Vol.65 No.3