HMG-CoA 환원효소 억제제에 의한 ICAM-1 유전자의 발현조절

김현진,정효균,홍우정,김군순,조영석,김도희,채수흥,구본정,송민호,노흥규,김영건 충남대학교 의과대학 지역사회의학연구소 2001 충남의대잡지 Vol.28 No.1

Background : ICAM-1 act as one of major adhesion molecules in the atherosclerotic lesion. ICAM-1 expression is mainly regulated at the level of transcription and depend on IFN-γ signal transduction pathway in which the STAT1 transcrption factor is a critical intermediate. IFN-γreceptor not only initiates tyrosine 701 phosphorylation of STAT1 by Jak1 and Jak2, but also phosphorylates serine 727 through the activation of Raf-1/MAP kinases. HMG-CoA reductase inhibitors have anti-atherosclertic effects, beyond normalization of hypercholesterolemia, by directly acting on endothelial cells, macrophages and vascular smooth muscle cells. HMG-CoA reductase inhibitors suppress the synthesis of isoprenoid intermediates such as geranylgeranyl-pyrophosphate or farnesylpyrophosphate. These effects results inhibitors suppress the synthesis of isoprenoid intermediates such as geranylgeranyl-pyrophosphate or farnesylpyrophosphate. These effects results inhibition posttranslational farnesylation and geranyl-geranylation processing of small GTP-binding preoteins and inhibition of normal signaling activities. Method : We made several 5'-deletion constructs of rat ICAM-1 promoter and analyzed the promoter activities by measuring the luciferase activity after transfection into ECV304 cells and smooth muscle cells. We checked the level of total and phosphorylated STAT1 protein by immunoblot analysis using specific antibodies. Results : Lovastatin inhibits IFN-γ-induced ICAM-1 gene expression in the ECV304cell. The cells pretreated with PD98059, MEKK inhibitor showed significantly low ICAM-1 RNA induction with IFN-γ stimulatio. IFN-γ induced phosphorylation of tyrosine 701 was not significantly changed by the pretreatment of lovastatin. But lovastatin suppresses IFN-γ-induced phosphorylation of ERK1/ERK2 which are responsible for the seine 727 phosphorylation in STAT1. Conclusion : We showed that HMG-CoA reductase inhibitors, lovastatin, suppresses IFN-γ mediated ICAM-1 gene expression through the inhibition of transcription. HMG-CoA reductase inhibitor suppresses IFN-γ-induced phosphorylation of serine 727 in STAT1 through the modulation of MAP kinases.

알코올의 섭취가 흰쥐의 혈액과 간의 지질대사에 미치는 영향

이명숙,김영연,김효숙,김매하,전진순,서진숙,송선미,양영재,윤원손,이미라,이효영,정승현 誠信女子大學校 生活文化硏究所 2004 生活文化硏究 Vol.18 No.-

To investigate the effects of ethanol on the lipid metabolism in blood and liver, twenty one male Sprague Dawley rats had orally taken the 25% alcohol and 50% alcohol by 5g per Kg of weight during 3 weeks after the adjusting period. For the energy balance, 85% sucrose and 45% sucrose was added in control group and 25% alcohol group each. The weight was reduced by alcohol treatment according to the levels, otherwise the weight of liver and heart was little increased in alcohol groups rather than in control without significance. Moreover, RBC, WBC, hemoglobin and hematocrit were increased by alcohol treatment according to the levels without significance. Otherwise, adjusted bilirubin levels by 100g of weight was significantly increased by alcohol treatment dose dependently. Alcohol induced RBC degradation and increased circulated bilirubin because of liver damage. For blood lipid profiles, blood cholesterol was increased in 50% of alcohol group compared to that of 25% and control groups, otherwise, blood triglycerides (TG) was increased by 30% in 25% alcohol groups rather than in control but not significantly different. The hepatic lipid composition, cholesterol levels in alcohol treatment groups was lower than those in control. The hepatic TG was not influenced by alcohol treatment. The two reasons of decreasing hepatic lipids were suggested by 85% sucrose of control for energy balance more synthesized cholesterol and TG than those in 25 or 50% alcohol groups, and by cholesterol and TG could not be synthesized because of liver damage from alcohol.

Increased Urinary KIM-1 and Tubular KIM-1 Expression in IgA Nephroapathy (초)

( Mi Seon Seo ),( Soon Hyo Kwon ),( Moo Young Park ),( Soo Jung Choi ),( Jin Seok Jeon ),( Hyun Jin Noh ),( Jin Gook Kim ),( Dong Cheol Han ),( Seung Duk Hwang ) 대한내과학회 2010 대한내과학회 추계학술대회 Vol.2010 No.-

대동맥 협착증과 동반된 출혈성 위장관 동정맥 기형에서 Estrogen-Progesterone 치료 1예

김도연,김화정,김효정,남준식,박수영,박영미,신정은,주미순,황민규,정혜경,손혜영,이선영,이한주 大韓消化器內視鏡學會 1999 Clinical Endoscopy Vol.19 No.부록2

Rhizopus oligosporus 를 이용한 한국된장의 제조에 관한 연구

김상순,박효숙 淑明女子大學校 生活科學硏究所 1988 論文集 Vol.2 No.1

In order to characterize chemical & physical changes of doenjang which was fermented by R.oligosporus, three kinds of doenjangs were prepared with R.oligosporus and Asp.oryzae. Among the three groups, the first group was prepared with Asp. oryzae, the second group was prepared with Asp.oryzae and R. oligosporus and the third group was prepared with R. oligosporus. Kojis were prepared with Asp. oryzae and R. oligosporus respectively, fermented for 48hr and enzyme activities of kojis were examined. Chemical & physical changes of each group of doenjangs were found during were found during 60 days of fermentation period. These groups of doenjangs were analyzed regularly at 10 days' interval and the results of the study were summarized as follows; 1. protease and amylase activities in Asp.oryzae koji and R. oligosporus koji were increased gradually during 48hrs of fermentation. Asp. oryzae koji showed higher enzyme activities during fermentation than that of R. oligosporus koji. 2. pH was reduced during the doenjangs fermentation in all of the three groups. The final pH value of the doenjangs showed from 5.66 to 5.92 at the last stage of fermentation. Especially the mixture group showed a large magnitude drop in pH compared to those of others over the 60-day test period. On the other hand titrable acidity was increased during the doenjangs fermentation. 3. The total nitrogen content was changed irregularly from 2.31 to 2.64% during the doenjangs fermentation. The each content of water soluble nitrogen, amino type nitrogen and ammonia type nitrogen was increased during the doenjangs fermentation. The content of nitrogen compounds of Asp. oryzae group was the highest among all of the three groups. 4. Total sugar content was decreased gradually, while the reducing sugar content was being remained below 1.5%. The reducing sugar content was increased for 20-30 days and was decreased after that period. 5. Free amino acid content was increased during the doenjangs fermentation and showed maximum content after 60 day's fermentation. The content of total free amino acid was the highest in Asp. oryzae group and showed the order of mixture group and R. oligosporus group. Histidine content was the highest, followed by Glutamic acid and Aspartic acid. 6. The L(Lightness) color values ware decreased while a(redness) and b(yellowness) were increased during the doenjangs fermentation in all of three groups. Change in L was the highest in R.oligosporus group, change in a & b was the highest in Asp. oryzae group. 7. In sensory evaluation, Asp. oryzae group showed the most excellent color, taste and total acceptability. There were not significant differences in taste and total acceptability between Asp. oryzae group and R. oligosporus group but was a significant difference between mixture group and the other two groups.

서양의복구성 부인원형 비교ㆍ 연구

김희진,조효순 명지대학교 자연과학연구소 1999 자연과학논문집 Vol.18 No.-

부인기본원형이 수록되어 있는 강순희의 「의복입체구성」, 도재은의 「패턴디자인의 제작법」, 이형숙의 「서양의복구성」등 교재 세편을 중심으로 부인원형을 제도. 제작하였다. 교재 세편에 나와있는 부인원형 중길. 소매. 스커트. 바지원형의 치수공식과 실측치수와의 차이 등을 비교하였다. 상의의 길원형에서 가슴 넓이는 「서양의복구성」과 「의복입체구성」에서의 여유분이 비슷하였으며, 가슴 포인트는 「패턴디자인 및 제작법」에서 유장의 길이가가장 짧았다. 허리둘레는 「의복입체구성」에서 가장 여유가 많았고 「패턴디자인 및 제작법」의 진동깊이는 다른패턴에 비해 가장 낮았다. 소매패턴제작에서는 「패턴디자인 및 제작법」에서 기준을 팔둘레로 잡아 제작하여 원형상의 보정이 필요하였다. 스커트 패턴제작시 「서양의복구성」과 「패턴디자인 및 제작법」의 스커트 경우는 엉덩이의 확대를 나타내는 시각적인 착시현상이 나타났다. 바지 패턴제도시 「서양의복구성」과 「의복입체구성」경우는 엉덩이 둘레에 비해 여유가 있었으나 「패턴디자인 및 제작법」의 경우는 엉덩이 둘레보다 적은 치수가 측정되었다. The analizing result of "GIL" pattern (Jacket). 1) The extra space of it's bust was much the same in "The construction of western costume" by Hyung-Sook Lee and "The cubic effect of costume " by Soon-Hee Kang. 2) The length between the both bust point was the most shortest. ("Pattern-design and manufacturing" by Jae-eun Do. 3) The waist circumference was more spacious than other portion of cubic construction. 4) The depth of armhole by jae-eun Do was the most deep among other patterns. 2. The analytic result of sleeves by the textbook of pattern-design and manufacturing (Jae-eun Do) revealed the need of correction on standardizing with arm circumference. 3. Seeing the analytic result of skirt, there was the effect of visual illusion that the waistline seemed to be thinner and slip from the central line in spite of the spaciousness in Western Clothing Construction, and also the buttock seemed to be large in the cubic construction, pattern-design and manufacturing method. 4. In the pattern-drafting of slacks, there was the spaciousness comparing with the real hip circumference in the case of western clothing construction and cubic construction of costume, but it was smaller size than the hip circumference in the case of pattern design and manufacturing method.

C형 니만-피크병의 유전자 치료를 위한 렌티바이러스 벡터의 개발

천효순, 김형규 충북대학교 동물의학연구소 2008 Journal of Biomedical and Translational Research Vol.9 No.3

본문참고

무형문화재 지정 현황 조사연구

김효순 경희대학교 체육대학 한국체육과학연구소 2002 體育學論文集 Vol.30 No.-

The purpose of this to Breach of Contract of Present Conditions Designation of intangible Cultural Property. Korean culture now danger losing its identity. Tradition has types of relationship with the society. When a society undergoes a rapid change in all aspects, its compatibility with culture may possibly be lost or weakened. that is, relationship between society and culture will be in crisis.

株式上場促進에 관한 考察

金孝順 梨花女子大學校 法政大學 1968 法政學報 Vol.11 No.-

섭취유지의 종류가 흰쥐의 혈청 및 간장의 지질성분에 미치는 영향

金君子,鄭孝淑,朴畢淑,姜晋順,鄭承鏞 慶尙大學校 1987 論文集 Vol.26 No.1

지방 산조성이 다른 수종의 유지를 흰쥐에 급이하여 혈청 및 간장중의 지질농도에 미치는 영향을 실험하여 다음과 같은 결과를 얻었다. 1. 식이섭취량과 증체량은 각 군간에 유의성이 없었다. 2. 혈청의 총 콜레스테롤 농도는 4군 (5% 라드+5% 말쥐치기름)은 높았다.HDL-콜레스테롤 농도는 각 국간에 큰 차이는 없으나 5군 (5% 라드+5% 말쥐치기름), 3군 (5% 라드+5% 콩기름)이 비교적 높았으며 총 콜레스테롤에 대한 HDL-콜레스테롤의 비율은 3군(5% 라드+5% 콩기름)이 가장 높았다. 3.간장의 총 콜레스테롤 농도는 각 군간에 큰 차이는 없으나 4군(5% 라드+5% 말쥐치기름)이 비교적 낮았으며 2군(5% 라드+5% 올리브기름)이 가장 높았다. 4. 혈청중의 중성지질 농도는 각 군간에 큰차이는 없으나 4군(5% 라드+5% 들깨기름), 5군(5% 라드+5% 말쥐치기름)이 약간 낮은 경향이었으며 인지질 농도는 3군 (5% 라드+5% 콩기름)이 비교적 낮았다. 5. 간장의 중성지질 농도는 대조군에 비하여 4군(5% 라드+5% 들깨기름), 5군 (5% 라드+5% 말쥐치기름), 2군(5% 라드+5% 올리브기름)이 비교적 낮았으며 3군(5% 라드+5% 콩기름)이 가장 높았다. 인지질 농도는 각 군간에 큰차이는 없으나 3군(5% 라드+5% 올리브기름)이 비교적 높았다. 6. 혈청 및 간장의 과산화지질 농도는는 대조군에 비하여 4군(5% 라드+5% 들깨기름),5군(5% 라드+5% 말쥐치기름)이 현저히 높았다. In order to investigate the aspect of nutritional physiology of various dietary fats and oils with different composition of fatty acids, it was experimented that the effect if dietary fats andoils on lipid concentration in serum and liver of rats. The results were as follows: 1. Body weight gain and amount of food intake were not significantly different among all groups. 2. Concentrations of total cholesterol in serum were significantly lower in group 4 (5% lard+ 5% perilla oil) and group 3 (5% lard + 5% soybean oil0 than control group(10% lard) 3. High density lipoprotein cholesterol concerntrations in serum were the highest in group 3 among all groups, the ratio of those to total cholesterol was significantly higher in group 3 and group 4 than control group. 4. Concentrations of total cholesterol in liver were remarkably lower in group 4 than any other groups. 5. Triglyceride concentrations in serum were significantly lower in group 4 and group 5 than other groups adn phosphospholipid concentrations were the lowest in griup 3 among all groups. 6. Concentrations of triglyceride and phospholipid in liver were the lowest in group 4 among all groups. 7. Concentrations of lipid peroxid in serum and liver were remarkably higher in group 4 and group 5( 5% lard +5% file fish oil) than control group.