젊은 남자 성인의 칼슘과 철의 영양상태에 관한 연구

박영숙,곽주영,권지혜,류화라 순천향대학교 기초과학연구소 2002 순천향자연과학연구 논문집 Vol.8 No.1

A survey was performed to 174 male young men who were living alone(40 persons) and shared living with friends(4143 persons) in Chungnam province. Their average age was 22.6 years old, height 171.8 ㎝, and weight 65.6 ㎏. Their daily calcium intake level was averaged 271.9±169.1 ㎎, which was 38.8 %RDA. Comparing calcium intake of each mealtime, breakfast, lunch, dinner, and snacks were composed of 18.6%, 30.0%, 34.0% and 17.4%, respectively. Their daily iron intake level was averaged 8.9±10.8 ㎎, which was 74.3 %RDA. Comparing iron intake of each mealtime, breakfast, lunch, dinner, and snacks were composed of 15.4%, 33.6%, 36.3% and 14.7%, respectively. These results suggested that deficiency of the two minerals in young male adults were severe and the nutrient qualities were also poor, no matter of their living alone or with accompanies. The deficiency was more profound in calcium than iron. And we observed iron nutriture differed significantly according to their living status, such as iron intake and its %RDA were significantly worse in single living subjects than the others. However calcium did not show significant difference. We concluded that shared living environment in young male adults could possibly improve their dietary mineral intake levels.

Helicobacter pylori와 대장균의 Shuttle Vector 개발

조명제,이우곤,이상룡,김경희,안영숙,김성희,김현주,류복덕,최여정,윤영혜,백승철,전영석,이광호 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-

In this study, a vehicle vector using cryptic plasmids was constructed for gene transfer in Helicobacter pylori. pHP51(3.9 kb) and pHP489(1.2 kb) were selected for constructing vectors from cryptic plasmid of H. pylori isolates in Korea. The HindⅢ-digestedDNA fragment(1.2kb) of pHP489 and 1.6kb DNA fragment of pHP51 were ligated with a kanamycin resistance gene(aph3'-Ⅲ) from C. jejuni to produce the recombinant plasmids pHP489K and pHP51K, respectively. Transformation frequency of pHP51K by electroporation was low. But pHP489K could be effectively transformed into various H. pylori strains. In order to design an intermdiate vehicle vector for gene transfer into H. pylori, pBlueHP489K was prepared by recloning pHP489K DNA into pBluescript and pTZ19R vector. This vector permitted the DNA fragment containing pHP489 sequence, aph3'-Ⅲ, and cloned DNA to be cut and self-ligated in the SacⅠ site after cloning. ureA and ureB gene were inserted into pBlueHP489K, resulting in pBlueHP489K/AB. The DNA fragment containing pHP489, kanamycin resistance gene(aph3'-Ⅲ), and urease structural gene was cut away from pBlueHP489K/AB and self-ligated to generate pBlueHP489K/AB. pBlueHP489K/AB made urease-negative H. pylori strains restore their urease activity. By this experiment, pBlueHP489K was confirmed to be the vehicle system for transferring H. pylori genes.

대동맥 협착증과 동반된 출혈성 위장관 동정맥 기형에서 Estrogen-Progesterone 치료 1예

김도연,김화정,김효정,남준식,박수영,박영미,신정은,주미순,황민규,정혜경,손혜영,이선영,이한주 大韓消化器內視鏡學會 1999 Clinical Endoscopy Vol.19 No.부록2

학교 지역문화교육을 위한 지역협의체 구성에 관한 연구

김영순,임지혜,배현주 한국문화교육학회 2011 문화예술교육연구 Vol.6 No.2

The aim of this study was to search conditions activating the regional consortium for regional culture education in schools. Results of this study are follows: the regional consortium consists of parents of students, communities, schools, school boards, public institutes, and NGO groups. The consortium contribute the educational support and development for student activities in schools. Furthermore the consortium make an offer to coordinate and facilitate the delivery of information, resources, and services to students, educators, employers, and the community. Therefore, the consortium might be best described as "matchmakers" that help ensure that our customers are able to easily connect with providers of the services they need. The consortium will be made up of a network of service providers from across its region that all collaborate in helping to connect the local education and school communities. 본 연구에서는 지역 사회의 인적·물적 자산을 활용한 문화적 체험활동 교육이 활성화되고 정착되기 위한 방안으로 학교와 지역사회가 밀접한 관계를 형성할 수 있는 지역협의체를 구성하는 안을 제시하였다. 이를 위해 지역문화교육에 필요한 지역협의체에 대한 문헌연구를 수행하였고, 이와 유사한 실제 사례를 찾아 관련 전문가를 대상으로 한 심층인터뷰 결과를 정리하고 시사점을 도출하였다. 연구결과, 지역협의체는 지역특수성, 전문성, 개방성, 공공성, 지속성의 기본원칙을 전제로 하여 교육행정기관, 지자체 행정기관, 민간 및 공공기관, 학교 등의 협력을 통해 구성될 수 있다. 또한 지역에서의 문화적 체험활동의 효율적인 운영을 지원하기 위해 1) 지역사회 내 교육기부 협력기관을 모색 및 발굴·연계, 2) 지역의 교육 인프라 접근성 향상, 3) 지역의 문화 교육 자원 간 네트워크 확장, 4) 지역 내 긍정적인 담론 형성을 위한 홍보 및 인식 개선, 5) 지역문화교육 프로그램 수행 과정의 성찰 등의 역할을 수행해야 한다. 지역협의체를 통한 학교와 지역교육자원의 연계 방안은 교육자원의 유형과 교육운영 주체에 따라 분류될 수 있었고, 강동구청과 대구광역시교육청의 예를 통해 지역문화교육 지원의 구체적인 사례를 살펴볼 수 있었다. 이와 같이 본 연구에서 제안한 지역협의체의 구성은 지역문화교육의 활성화를 위한 초석을 마련해 줄 것이라고 기대해 본다.

췌장의 점액성 낭선암 1예

송현주,김도영,정혜경,남승현,문일환,구혜수 梨花女子大學校 醫科大學 醫科學硏究所 2002 EMJ (Ewha medical journal) Vol.25 No.2

Nowadays increasing use of abdominal ultrasound in routine check-up may increase the detection rate asymtomatic cystic lesions of pancreas. Even through the majority of the cystic lesions of pancreas is pseudocyst, about 10-15% of those lesions are caused by pancreatic cystic tumor. In the pancreatic cystic tumor, especially, mucinous cystic tumor should be exicised due to its malignant potential, while the pancreatic pseudocyst or serous cystic tumon can be observed for a period or treated medically. Several clinical, radiological, biochemical and pathologic guidelines have been developed in order to distinguish among them. Among pancreatic cystic tumors, mucinous cystic adenocarcinoma is very rare and accounts for only 1% of all pancreatic neoplasms. Unlike extremely poor prognosis of pancreatic adenocarcinoma, mucinous cystic adenocarcinoma has an indolent course and shows a good prognosis after its curative resection. Recently we experienced a 69-year-old woman who had a mucinous cystic adenocarcinoma of the pancreas. We report this case with a review of literature.

방사선 치료를 받는 암 환자들의 사회적 지지와 삶의 질과의 관계

정주희,류소연,윤혜은,남택근,오윤경,안현옥,박계남,이영선 朝鮮大學校 附設 醫學硏究所 2002 The Medical Journal of Chosun University Vol.27 No.2

Objective : This study was performed to investigate the relationship between social support and quality of life among cancer patients receiving radiation therapy. Matehals and Methods : The data were collected from 98 patients, who were receiving radiation therapy at two university hospitals located in GwangUJu, used by structured questionnaire. For statistical analyses of the association between quality of life and various characteristics, data were analyzed using t-test, ANOVA, Pearson 's correlation, and multiple regression analysis. Results: 1. There were 56(57.1%) males and 42(42.9%) females. Age ranged from 21 to 82 years. The primary sites of cancer were gastrointestinal tract (24.5%), lung (23.5%), breast (21.4%), and head and neck (11.2%) in order. 2. The mean scores of social, family, and medical support were 4.30 0.58, 4.49 0.78, 4.11 0.65, respectively. The score of quality of life was 5.83 1.63 (range: 1.95 ~ 9.05). 3. An analysis of the association between several factors of patients and quality of life showed that the statistically significant factors were age, the presence of distant metastasis, family support, medical support and social support. 4. As a result of the multiple regression analysis, only social support was significant (β=0.932, P=0.02) with quality of life, but age and presence of distant metastasis were not significant. Conclusion : This suggests that quality of life in cancer patients could be improved by strengthening the social support which consists of family and medical support. Further study would be necessary to evaluate separately several aspects of quality of life among cancer patients.

병·의원에서 분리한 메티실린 내성 황색포도알균에서 SCCmec 유형과 Aminoglycoside 변형효소 유전자의 분포

정영희,김광욱,차정옥,이경민,유재일,유정식,김봉수,노영주,윤혜령,이영선 대한감염학회 2008 감염과 화학요법 Vol.40 No.1

목적 : 대부분의 AME 효소 유전자는 transposon이나 plasmid 상태로 전달되며 staphylococcal chromosomal cassette mec (SCCmec) 내에도 삽입되어 있다. 최근 MRSA의 SCCmec 유형에 따라 다양한 내성유전자를 가지는 것으로 보고되고 있다. 따라서 1, 2차 의료기관에서 분리된 황색포도알균의 항생제 내성률을 조사하고 MRSA에서 SCCmec type에 따른 AME 효소 유전자의 분포를 확인하였다. 재료 및 방법 : 2004년 1, 2차 의료기관에서 분리된 황색포도알균 640주를 대상으로 methicillin의 내성유전자 mecA와 AME 효소 유전자 aac(6')-aph(2"), aph(3')-IIIa, ant(4')-Ia 등을 multiplex PCR 방법을 이용하여 확인하였다. 항생제 감수성 시험과 MIC 값은 한천배지 희석법으로 확인하였고 SCCmec type도 결정하였다. 결과 : 640주의 황색포도알균 중 MRSA을은 39.7%였으며 MRSA 분리주 모두 mecA 유전자가 검출되었다. Aminoglycoside 중 kanamycin, tobramycin에 대한 내성률은 98.1%이었고, gentamicin 68.7%, amikacin 30.8%, netilomicin 2.8%이었으며 vancomycm에는 모두 감수성이었다. Oxacillin MIC_50과 MIC_90은 각각 128 ug/mL, 256 ug/mL이었으며 254주의 MRSA 중 214주를 선별하여 AME 효소 유전자를 확인한 결과 aph(3')-IIIa 13.1%, aad(6')-aph(2") 77.1%, ant(4)-Ia 53.3%이었으며 SCCmec type에 따라서는 type II, type III, type IV가 각각 50.9%, 16.4%, 32.7%였다. SCCmec type에 따른 AME 효소 유전자의 분포는 SCCmec type II에서는 aac(6')-aph(2")와 aac(6')-aph(2")/ant(4')-Ia가 각각 49.5%, 36.7%에서 검출되었고 type III에서는 aph(3')-IIIa/aac(6')-aph(2")가 60%, aac(6')-aph(2")가 31.4%, type IV에서는 aac(6')-aph(2")/ant(4')-Ia와 ant(4')-Ia가 각각 41.4%, 50%로 나타났다. 결론 : 1, 2차 의료기관에서 분리한 황색포도알균 분리주의 메티실린 내성률은 39.7%이었다. MRSA 분리주 중 SCCmec type II와 III에서는 aac(6')-aph(2")가, SCCmec type IV에서는 ant(4')-Ia가 90% 이상 검출되어 SCCmec type과 AME 효소 유전자 분포와는 연관성이 있는 것으로 나타났다. Background : Many genes encoding aminoglycoside modifying enzymes (AMEs) on transposon or plasmid were transferred from one strain to another strain and inserted into a staphylococcal chromosomal cassette mec (SCCmec). There are very diverse subtypes in SCCmec type to the insertion of resistant genes. Therefore, we researched the resistance rates of antibiotics and distribution of AME genes according to SCCmec type in MRSA strains. Materials and Methods : We isolated 640 Staphylococcus aureus from non-tertiary hospitals in 2004, detected mecA, aac(6')-aph(2"), aph(3')-llla, and ant(4')-la using the multiplex PCR method, tested antibacterial susceptibility disk diffusion and minimal inhibitory concentration, and determined SCCmec type. Results : Of 640 S. aureus isolates, MRSA rate was 39.7% and all MRSA isolates carried mecA gene. Among 214 MRSA selected, aminoglycoside-resistant rates were 98.1% in kanamycin and tobramycin, 68.7% in gentamicin, 30.8% in amikacin, and 2.8% in netilmicin. The detection rates for aac(6')-aph(2"), aph(3')-llla, and ant(4')-la were 77.1%, 13.1%, and 53.3%, respectively. Also, SCCmec type was 50.9% in SCCmec type II, 16.4% in type Ill, and 32.7% in type IV. The genes encoding AMEs were distributed aac(6')-aph(2") (49.5%) and aac(6')-aph(2")/ant(4')-la (36.7%) in SCCmec type II, aph(3')-llla/aaac(6')-aph(2") (60%) and aac(6')-aph(2") (31.4%) in type III, and aac(6')-aph(2")/ant(4')-la (41.4%) and ant(4')-la (50%) in type IV. Conclusion : 39.7% of S. aureus isolated from non-tertiary hospitals was resistant to methicillin. More than 90% of MRSA isolates were detected aac(6')-aph(2") in SCCmec type II and Ill, and ant(4')-la in type IV. With these results, the genes encoding AMEs may be closed related to SCCmec type.

‘병동 중심의 처치수가 산정지침 개발’ 등을 통한 간호업무 표준화 및 그 효과

한혜정,설미진,김영주,박소영,박아리스,문한경,이현영 한국의료QA학회 2009 한국의료질향상학회지 Vol.15 No.1

문제: 병동 처치수가 산정 부정확 및 누락으로 인한 부적절한 재고관리로 진료차질, 불필요한 업무발생 및 수익이 감소한다. 목적: 병동 처치수가 산정 정확화를 위한 업무 표준화 도구 및 시스템을 개발하여 적정 재고관리를 통한 수익증대 및 직무만족도를 향상시킨다. 의료기관: 서울시 종로구에 소재한 대학병원 질 향상 활동: 병동중심의 처치 산정지침 개발 및 수가물품의 적정재고 관리방안을 모색하였다. 개선효과: 병동중심의 처치수가 산정 지침서를 제작하여 업무표준화를 기하였음. 응급청구 품목 및 수량이 ’07년 대비 71%감소하고 타 병동 차용품목이 활동 전에 비해 61% 감소, 수량은 77% 감소함. 응급청구 총소요시간이 활동 전에 비해 ’07년 대비 77% 감소하고 타 병동 차용 총 소요시간이 61% 감소함. 전년 동기간 대비 수익이 4% 증가하였으며 또한 54병동과 보험 심사팀에서 직원들의 직무만족도가 향상되었다.

Immunoblotting을 이용한 Herpes Simplex Virus Type 1과 Type 2에 대한 특이 혈청 항체의 감별

박혜경,서주영 이화여자대학교 생명과학연구소 1994 생명과학연구논문집 Vol.5 No.-

There are two serotypes of herpes simplex virus, type 1 and type 2. HSV type 1 usually causes oropharyngeal infection while type 2 usually genitourinary infection. However, the clinical discrimination between two serotypes in eliciting disease entities is becoming uncertain. Furthermore, clinical discrimination is sometimes regarded of no importance due to lack of difference in treatment. However, serotyping is required for explicit diagnosis or epidemiologic survey. The conventional serological assay such as ELISA can not discriminate between HSV type 1 and type 2. Thus we tried to discriminate serum antibodies against HSV type 1 and type 2 by immunoblot analysis and compared it with the results of dot blot assay. Sera were separated from pheripheral blood of 10 patients who visited the department of dermatology or gynecology, Mok-Dong Hospital, Ewha Womans University from Jun. to Oct. 1994 for symptoms suspicious of genital herpes, 31 normal adult women, 20 pregnant women and 48 infants or children. Immunoblot analysis of HSV type 1 and type 2 revealed various patterns according to sera, not showing typical specific patterns. However, the two types could be differentiated in that type 1 frequently showed 134, 120, 106, 76, 71, 58, 50, 47kDa immunoblot bands, while type 2 showed 182, 126, 115, 109, 102, 95, 85, 81, 67kDa bands, frequently. Serum antibody positive rates against HSV type 1 and type 2 assayed by dot blot and immunoblot were contradictory in some cases. And it was false positive cases in dot blot for anti-HSV type 2 antibody which was most frequent, showing crossreaction dut to common antigenicity in whole virion antigen.

국내 독감백신 투여현황과 투여 결정에 영향을 주는 요인에 관한 연구

정주연,정선영,곽혜선 이화여자대학교 약학연구소 2005 藥學硏究論文集 Vol.- No.15

This study was aimed to determine influenza vaccination coverage in 2004 in Korean and investigate the factors associated with vaccination. Documentation of vaccination status and baseline data was conducted by a survey using questionnaire sheets. Baseline data and vaccination status were documented on 1465 people out of whom 60.1% received the influenza vaccine. Forth-seven percent of the responders were male; 4% were aged 1-12, 32% were aged 13-39, 32% were aged 40-64 and 32% were aged 65 or older. Twenty-three percent reported a chronic illness, increasing their risk for complications from influenza. Predictors of influenza vaccination were: older age (OR=11.7, 95% CI 5.1-26.8), the presence of chronic illness (OR=2.3, 95% CI 1.1-4.7), previous vaccination(OR=1.8, 95% CI 1.1-2.8), belief that influenza vaccine is effective in preventing influenza (OR=2.5, 95% CI 1.1-5.7) and education level (OR=1.7, 95% CI 1.0-2.7). Immunization rates were much higher in those who will take immunization again (OR=10.4 95% CI 5.5-19.6). Factors affecting the decision on immunization were self-determination (43.6%), public relations (24.1%), recommendation from family members or friends (22.4%) and consulting with health professionals (5.8%). The main reason not to take influenza vaccine was the thought that they are healthy (50.1%). Overall, influenza vaccine coverage was high in those aged 65 or older. Immunization against influenza was influenced more by existing medical problem and belief about th vaccine's effectiveness, rather than sex or residence.