보리순의 영양성분 조성

서혜지,최용민,김상천,이준수,이기택,정명근,천지연,황진봉,남진식,서우덕,황영,김행란,김세나 忠北大學校 農業科學硏究所 2015 農業科學硏究 Vol.31 No.2

발열로 인해 발현된 부루가다 증후군 1례

박혜연,김종훈,박경일,황철웅,김태년,남궁준,도준형,이원로,이성윤 白中央醫療院 2005 仁濟醫學 Vol.26 No.1

Brugada syndrome is characterized by right bundle branch block morphology and ST segment elevation in the right precordial leads and a propensity to develop ventricular arrhythmias. Mutations in a cardiac sodium channel gene have been linked to this syndrome, and the ionic mechanisms responsible for the electrocardiographic phenotype are temperature-dependent. This case report describes a patient in whom a typical Brugada ECG pattern developed during fever and returned normal ECG after fever was subsided.

Streptomyces exfoliatus로부터 얻어진 제한효소의 분리정제 및 성질에 관한 연구

배무,박정옥,황혜연,임정빈 이화여자대학교 생명과학연구소 1993 생명과학연구논문집 Vol.4 No.-

30가지의 방선균 균주를 대상으로 제한효소를 탐색하였는데, 이 중 한 균주 (Streptomyces exfoliatus)로부터 제한효소의 활성을 발견하여 이로부터 제한효소 sexⅢ를 분리제정하고 그 특성을 조사하였다. 제한효소의 정제는 streptomycine sulfate 침전, DEAE-cellulose, hydroxylapatite, phosphocellose PⅡ column chromatography에 의해 행해졌고, SDS-polyacrylamide gel electrophoresis에 의해 소단위체의 분자량이 56,000 dalton으로 추정되었다. 이 효소는 lambda DNA를 네 군데, pBluescript를 한 군데 자른다. 활성은 넓은 범위의 pH에서 안정되었고 75mM이상의 염에 의해서 저해된다. 활성을 위해 고농도(20-40 mM)의 MgCl_2가 요구되며 다른 효소들과 달리 낮은 농도의 MgCl_2에서는 부분절단이 일어나는 것이 특징이었다. Lambda DNA를 이용하여 제한효소의 지도를 작성한 결과 인식서열이 5'-CCGC/GG-3'으로 밝혀졌으므로 이 sexⅢ는 saeⅡ의 isoschizomer임이 확인되었다. Thirty strains of Streptomyces sp.isolated from soil samples were screened for the presence of site-specific endonuclease. One strain among them showed endonuclease activity to cleave lambda DNA. This study describes the purification and characterization of a site-specific restriction endonuclease SexⅢ from Streptomyces exfoliatus. The purification procedure includes streptomycin sulfate treatment, DEAE-cellulose, hydroxylapatite, phosphocellulose PⅡ column chromatography. This enzyme required high Mg^2+ concentration (20-40 mM). and showd maximal activity at neutral ph(7-8) in the absence of NaCl. The enzyme did not require salt for its activity and was inhibited by over 75mM NaCl. SexⅢ cut lambda DNA and plasmid pBluescript, recognized the hexanucleotide sequence 5'-CCGC/GG-3', and proved to be an isoschizomer of SacⅡ.

Streptomyces diastatochromogenes로부터 분리된 SdiⅠ의 특성에 관한 연구

배무,송은숙,황혜연,임정빈 이화여자대학교 생명과학연구소 1994 생명과학연구논문집 Vol.5 No.-

토양분리 방선균 Streptomyces diastatochromogenes로부터 분리된 제한효소 SdiI은 넓은 범위의 pH(7.0~1.5)와 온도(-60℃)에서 활성을 보였으며, 고농도(-500mM) NaCI이 있어도 작용하였다. 또한, 20~60℃ 온도에서 안정하며, 활성을 갖기 위해서는 MgCI_2를 필요로 하였다. Lambda DNA에 대한 지도 작성으로부터 XhoI의 isoschizomer로 추정되었으며, DNA 염기서열 분석 결과, 인식, 절단 서열은 다음과 같이 결정되었다. In catalytic propeties of the restriction endonuclease. SdiI, which was purified from Streptomyces diastatochromogenes, this enzyme was active at wide range between pH 7.0 and 12.5, and up to 60℃ and 500mM of NaCl concentration. It was stable between 20℃ and 60℃, and essentially requires MgCl_2 for endonuclease activity. The restriction map of lambda DNA which was obtained by double digestion with various enzymes suggested SdiI to be an isoschizomer of XhoI. From the determination of restriction site based on DNA sequencing method, recognition and cleavage specificity of SdiI was concluded as:

급성 호흡곤란으로 내원한 성대기능이상 1례

함초롬,박경일,박혜연,황철웅,김종연,김태년,진재용,이성순 白中央醫療院 2005 仁濟醫學 Vol.26 No.1

Vocal cord dysfunction (VCD) is a respiratory disorder characterized by the abnormal adduction of the vocal cords upon inspiration, expiration, or both, leading to a diverse array of acute obstructive airway signs and symptoms (wheezing, chest tightness, shortness of breath, and cough). VCD frequently mimics or confounds asthma. Misdiagnosis as asthma has led to inappropriate treatment, most notably with high-dose corticosteroids. Mistaken for anaphylaxis or angioedema, VCD has led to intubation and tracheostomy. VCD should be suspected in patients who are treated aggressively for asthma but continue to experience dyspnea. The diagnosis of VCD is made from direct visualization of the vocal cords during an attack. Inspiratory, anterior vocal cord closure with a posterior glottic chink is seen. On pulmonary function testing, a pattern of "saw-toothing", or fluttering of the inspiratory limb, representing fluctuations in the abnormal cord motion is seen in VCD patient. Treatment includes discussion of the diagnosis with the patients, discontinuing unnecessary medications, using of a helium-oxygen mixture in acute attack. Speech therapy and psychotherapy are the mainstays of long-term treatment. Here in we report a case of VCD in company with asthma.

패혈증 이후 발생한 스트레스성 심근병증 2례

김종훈,남궁준,박혜연,황철웅,박경일,도준형,이성윤,이원로 白中央醫療院 2005 仁濟醫學 Vol.26 No.1

The transient left ventricular apical ballooning syndrome, also known as takotsubo cardiomyopathy is a recently described novel cardiac syndrome. This syndrome is characterized by transient asynergy of the ventricular apex or mid-ventricle in the absence of obstructive epicardial coronay artery disease. This report concerns two types of morphologic difference about left ventricular apex and mid-ventricle. In this case, we report two types of stress-induced cardiomyopathy with review of literatures.

Clopidogrel에 의해 발생된 전신 염증 반응 증후군 1례

김민환,김종훈,박경일,박혜연,황철웅,김의석,도준형,남궁준,이성윤,이원로 白中央醫療院 2005 仁濟醫學 Vol.26 No.1

Clopidogrel bisulfate, a widely used inhibitor of platelet aggregation, is considered at least as safe as aspirin. We describe a 61 year old male patient who developed a systemic inflammatory response syndrome consisting of high fever, rash, chills, impaired liver function, and mild leukopenia after receiving clopidogrel after coronary angiography and stent implantation. The reaction resolved promptly after withdrawal of the drug, thus making the diagnosis of a clopidogrel induced reaction highly probable.

Quantitative Vertebral Bone Density Seen on Chest CT in Chronic Obstructive Pulmonary Disease Patients: Association with Mortality in the Korean Obstructive Lung Disease Cohort

Hwang Hye Jeon,이상민,Seo Joon Beom,Kim Ji-Eun,Choi Hye Young,Kim Namkug,Lee Jae Seung,Lee Sei Won,Oh Yeon-Mok 대한영상의학회 2020 Korean Journal of Radiology Vol.21 No.7

Objective: Patients with chronic obstructive pulmonary disease (COPD) are known to be at risk of osteoporosis. The purpose of this study was to evaluate the association between thoracic vertebral bone density measured on chest CT (DThorax) and clinical variables, including survival, in patients with COPD. Materials and Methods: A total of 322 patients with COPD were selected from the Korean Obstructive Lung Disease (KOLD) cohort. DThorax was measured by averaging the CT values of three consecutive vertebral bodies at the level of the left main coronary artery with a round region of interest as large as possible within the anterior column of each vertebral body using an in-house software. Associations between DThorax and clinical variables, including survival, pulmonary function test (PFT) results, and CT densitometry, were evaluated. Results: The median follow-up time was 7.3 years (range: 0.1–12.4 years). Fifty-six patients (17.4%) died. DThroax differed significantly between the different Global Initiative for Chronic Obstructive Lung Disease stages. DThroax correlated positively with body mass index (BMI), some PFT results, and the six-minute walk distance, and correlated negatively with the emphysema index (EI) (all p < 0.05). In the univariate Cox analysis, older age (hazard ratio [HR], 3.617; 95% confidence interval [CI], 2.119–6.173, p < 0.001), lower BMI (HR, 3.589; 95% CI, 2.122–6.071, p < 0.001), lower forced expiratory volume in one second (FEV1) (HR, 2.975; 95% CI, 1.682–5.262, p < 0.001), lower diffusing capacity of the lung for carbon monoxide corrected with hemoglobin (DLCO) (HR, 4.595; 95% CI, 2.665–7.924, p < 0.001), higher EI (HR, 3.722; 95% CI, 2.192–6.319, p < 0.001), presence of vertebral fractures (HR, 2.062; 95% CI, 1.154–3.683, p = 0.015), and lower DThorax (HR, 2.773; 95% CI, 1.620–4.746, p < 0.001) were significantly associated with all-cause mortality and lung-related mortality. In the multivariate Cox analysis, lower DThorax (HR, 1.957; 95% CI, 1.075–3.563, p = 0.028) along with older age, lower BMI, lower FEV1, and lower DLCO were independent predictors of all-cause mortality. Conclusion: The thoracic vertebral bone density measured on chest CT demonstrated significant associations with the patients’ mortality and clinical variables of disease severity in the COPD patients included in KOLD cohort.

Antimicrobial activity of submerged culture extracts from Talaromyces purpurogenus

HYE JIN HWANG,Hye Yeon MUN,Young Ho NAM,Jaeduk GOH,Eu Jin CHUNG 한국생물공학회 2019 한국생물공학회 학술대회 Vol.2019 No.10

Generalized Tonic-Clonic Seizures after Self-Limited Epilepsy with Centrotemporal Spikes: A Case Series

Hye Jin Kim(Hye Jin Kim),Young Joon Ko(Young Joon Ko),Soo Yeon Kim(Soo Yeon Kim),Anna Cho(Anna Cho),Hunmin Kim(Hunmin Kim),Byung Chan Lim(Byung Chan Lim),Hee Hwang(Hee Hwang),Jong-Hee Chae(Jong-Hee Ch 대한소아신경학회 2022 대한소아신경학회지 Vol.30 No.4

Purpose: Patients with self-limited epilepsy with centrotemporal spikes (SLECTS) rarely experience generalized tonic-clonic seizures (GTCS) after remission, and post-remission GTCS has not been thoroughly described in earlier studies. Herein, we describe the clinical and electrographic features of GTCS after a substantial period of seizure freedom in patients with SLECTS. Methods: This study included six patients (three boys and three girls) diagnosed with SLECTS who later developed GTCS after or near remission. Medical records, including clinical data and serial electroencephalography (EEG) recordings, were retrospectively reviewed for all patients. Results: Patients’ age at SLECTS onset ranged from 5.2 to 10.2 years (mean, 8.4 years), while seizure cessation was achieved between 8 and 12.2 years. During SLECTS, typical centrotemporal spikes were observed in all patients, and generalized spike-and-wave discharges were observed in three patients. The age at the first episode of subsequent GTCS ranged from 14.4 to 17.3 years (mean, 15.8 years), constituting an average interval of 5.6 years after the last episode of seizures (range, 4.1 to 8.1 years). EEG at subsequent episodes of GTCS revealed generalized discharges in two patients, focal discharges in two other patients, and normal discharges in the remaining two patients. Two patients had multiple episodes of GTCS. Conclusion: Although rare, GTCS may occur near or after remission in patients with SLECTS, and clinicians should be aware of this. Subsequent GTCS may be a manifestation of idiopathic generalized epilepsy. However, large-scale studies are needed to determine the nature of such episodes of GTCS and their associated risk factors.