Helicobacter pylori와 대장균의 Shuttle Vector 개발

조명제,이우곤,이상룡,김경희,안영숙,김성희,김현주,류복덕,최여정,윤영혜,백승철,전영석,이광호 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-

In this study, a vehicle vector using cryptic plasmids was constructed for gene transfer in Helicobacter pylori. pHP51(3.9 kb) and pHP489(1.2 kb) were selected for constructing vectors from cryptic plasmid of H. pylori isolates in Korea. The HindⅢ-digestedDNA fragment(1.2kb) of pHP489 and 1.6kb DNA fragment of pHP51 were ligated with a kanamycin resistance gene(aph3'-Ⅲ) from C. jejuni to produce the recombinant plasmids pHP489K and pHP51K, respectively. Transformation frequency of pHP51K by electroporation was low. But pHP489K could be effectively transformed into various H. pylori strains. In order to design an intermdiate vehicle vector for gene transfer into H. pylori, pBlueHP489K was prepared by recloning pHP489K DNA into pBluescript and pTZ19R vector. This vector permitted the DNA fragment containing pHP489 sequence, aph3'-Ⅲ, and cloned DNA to be cut and self-ligated in the SacⅠ site after cloning. ureA and ureB gene were inserted into pBlueHP489K, resulting in pBlueHP489K/AB. The DNA fragment containing pHP489, kanamycin resistance gene(aph3'-Ⅲ), and urease structural gene was cut away from pBlueHP489K/AB and self-ligated to generate pBlueHP489K/AB. pBlueHP489K/AB made urease-negative H. pylori strains restore their urease activity. By this experiment, pBlueHP489K was confirmed to be the vehicle system for transferring H. pylori genes.

Isolated intracranial fibromuscular dysplasia of the posterior cerebral artery

Yeo, Min-Ju,Jeon, Ho-Yeong,Cha, Min-Ju,Jang, Hye-Yeon,Lee, Sang-Gil,Seon Kim, Ji,Lee, Sung-Hyun,Lee, Sang-Soo,Shin, Dong-Ick Elsevier 2017 JOURNAL OF CLINICAL NEUROSCIENCE Vol.43 No.-

<P><B>Abstract</B></P> <P>Fibromuscular dysplasia (FMD) is a nonatherosclerotic, noninflammatory arterial disease that most commonly affects the renal and internal carotid arteries, but intracranial FMD is very rare. We report a patient with FMD involving the posterior cerebral arteries (PCAs). A 24year old female was presented with a 4day history of right homonymous hemianopsia with throbbing headache in the left temporo-occipital area. The brain magnetic resonance imaging (MRI) revealed an acute ischemic stroke in the left PCA territory, while the Time of Flight (TOF) magnetic resonance angiogram (MRA) showed segmental luminal irregularities in the left proximal PCA. The conventional angiogram revealed the “string of beads” appearance, a characteristic that is pathognomonic for FMD. The patient’s inhospital clinical course was stable, while there was no recurrence of stroke. This is the second report of FMD of the PCA. Notwithstanding incredibly rare incidences of isolated intracranial FMD and nonspecific findings of MRA, such a pathophysiology should be considered as the cause for a stroke in young patients, especially those with no cardiovascular risk factor.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Isolated intracranial FMD with the “string of beads” appearance is very rare. </LI> <LI> In young patients without the risk of atherosclerosis, intracranial FMD should be considered as a pathophysiology of stroke. </LI> <LI> TOF-MRA may show unclear “string of beads” appearance such as segmental luminal irregularities in intracranial FMD. </LI> </UL> </P>

Dental Hygienist Poster Presentation : A Study on the Dental Hospital Selection Motives of Implant Patients

( Yeo Ju Song ),( Su Hyun Kim ),( Sung Yeon Cho ),( Sun Ja Lee ),( Woon Jung Kim ),( Hye Sun Park ),( Min Kyung Yeo ),( Kyung Mi Yeo ),( Tae Rang Kim ),( Min Ji Han ),( Seong Ho Choi ) 대한치주과학회 2009 대한치주과학회 학술대회자료집 Vol.2009 No.1

Slide Session : OS-110 ; Critical Care : Comparison of Clinical Outcomes between Conventional and Conservative Anticoagulation Strategy for Extracorporeal Membrane Oxygenation in Adults: A Single-Center Experiences

( Hye Ju Yeo ),( Dong Wan Kim ),( Seong Hoon Yoon ),( Seung Eun Lee ),( Woo Hyun Cho ),( Bong Soo Son ),( Do Hyung Kim ),( Doosoo Jeon ),( Yun Seong Kim ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: Because acute respiratory failure often results in irreversible pulmonary damage with high mortality, lung transplantation might be one of the last option for these patients. However, there are few reports about this issue. Methods: From January 2011 to December 2013 we retrospectively reviewed respiratory failure patients with extracorporeal membrane oxygenator (ECMO) support and subsequently treated with lung transplantation. Results: Seventeen patients underwent lung transplantation surgery from irreversible respiratory failure during ECMO support. Seven patients were female and mean age was 44.5 ± 12.1 years. Overall observation duration was 534.2 ± 469.3 days. Ten patients had acute respiratory failure and 7 patients had chronic respiratory failure. Although the disease entity of the both groups was distinct, the duration of treatment with ECMO, intensive care unit stay and hospital stay, and clinical outcomes of acute respiratory failure patients were comparable to the chronic respiratory failure with ECMO. (Table 1) Conclusions: Lung transplantation in patients with acute respiratory failure with ECMO support exhibits comparable results to those with chronic respiratory failure with ECMO.

New Biomarker in Rapid Progressor of Idiopathic Pulmonary Fibrosis

( Hye Ju Yeo ),( Woo Hyun Cho ),( Yun Hak Kim ) 대한결핵 및 호흡기학회 2021 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.129 No.-

Background There are two subgroups of patients with idiopathic pulmonary fibrosis (IPF) who display a rapid progression to an end-stage disease or not. The molecular mechanisms underlying these two phenotypes, slow progressors (SP) and rapid progressors (RP) are poorly understood. Methods We identified differentially expressed genes (DEGs) in RP by conducting RNA sequencing from 3 SP, 3 RP, and 3 control lungs. We compared our Results to previously published datasets (GSE10667 and GSE 24206) and identified overlapping genes among DEGs from each dataset. We verified the genes expression through immunohistochemistry (IHC), western blot, and enzyme-linked immunosorbent assay (ELISA) in other IPF patients. Results Sixteen genes were overlapped from three datasets. Gene validation was conducted only for 9 genes. CD276, COL7A1, CTSB, GLI2, PIK3R2, PRAF2, IGF2BP3, and NUPR1 have up-regulated genes in RP, and ADAMTS8 was a down-regulated gene in RP. In IHC, staining of GLI2 and PIK3R2 in bronchial epithelial cells and basal cells was decreased in RP compared to SP, but staining of CTSB in macrophages was increased in RP compared to SP. In western blot, CTSB was increased in RP compared to control and SP. In serum ELISA, TGF-β was increased in RP compared to control and SP. IL-6 was increased in RP compared to control. CTSB levels were increased in RP compared to control and SP. Conclusion our Results indicate that RP is characterized by enhanced macrophage activation, as reflected by increased CTSB. The increase of CTSB in the peripheral blood and lungs may suggest their use as biomarkers for this phenotype.

Free Paper Presentation : OS-110 ; Comparison of Clinical Outcomes between Conventional and Conservative Anticoagulation Strategy for Extracorporeal Membrane Oxygenation in Adults: A Single-Center Experiences

( Hye Ju Yeo ),( Dong Wan Kim ),( Seong Hoon Yoon ),( Seung Eun Lee ),( Woo Hyun Cho ),( Bong Soo Son ),( Do Hyung Kim ),( Doo Soo Jeon ),( Yun Seong Kim ) 대한결핵 및 호흡기학회 2014 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.118 No.-

Background: Anticoagulation is a key component in running extracorporeal membrane oxygenation (ECMO). Anticoagulation, however, is related with serious bleeding complications. Previous studies suggested that bleeding complications are more commonly related to serious morbidity and mortality than thromboembolic ones. In this study, we hypothesized that lower dose of anticoagulation may be safe and effective and assessed the clinical outcomes according to different anticoagulation strategies. Methods: We reviewed retrospective data of ECMO database from December 2008 to March 2014. In the conventional group, anticoagulation targeted to maintain an activated clotting time (ACT) in between 180 and 220 seconds by a continuous infusion of unfractionated heparin. In the conservative group, anticoagulation targeted to activated partial thromboplastin time (aPTT) in between 50 and 70 seconds. We assessed the incidence of bleeding events, transfusion requirements, thromboembolic complications, and other clinical outcomes in each strategy. Results: A total of 626 days on ECMO in 58 patients were reviewed. The 59 percent of the patients receiving ECMO therapy were successfully weaned and the overall mortality was 50 percent. Overall, 40 percent of the patients (n=23) were treated with conventional anticoagulation strategy (S group) and 60 percent of the patients (n=35) were treated with conservative anticoagulation strategy (C group). Incidences of major bleeding and bleeding related death were significantly lower in the C group (57% versus 29%, p=0.033 and 13% versus 0%, p=0.028). The average daily transfusion doses of RBC unit and FFP were higher in the S group (3.4 versus 1.4, p=0.001, 2 versus 0.6, p=0.000). Conclusions: In this study, conservative anticoagulation strategy showed acceptable results in terms of major bleeding event, transfusion dose and thromboembolic complications. Such results make conservative anticoagulation strategy capable of reducing bleeding complications without serious thrombotic complication.

Periostin is a Novel Biomarker for Chronic Lung Allograft Dysfunction after Lung Transplant

( Hye Ju Yeo ),( Woo Hyun Cho ) 대한결핵 및 호흡기학회 2021 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.129 No.-

Background chronic lung allograft dysfunction (CLAD) is the main cause of poor long-term outcomes after lung transplantation. Until now, no specific biomarker exists. Methods We analyzed differentially expressed genes (DEGs) of 4 paired human lung tissue samples at the different time points of donor and CLAD state. Frozen fresh lung tissue and serum from Pusan National University Yangsan Hospital biobank were used for validation of identified potential biomarkers. Results We identified 20 hub-genes among DEGs (POSTN, COL1A, COL1A2, CXCL10, COL5A2, BGN, CCL5, STAT1, CXCL9, CXCR3, COL6A3, COL5A1, MX1, IDO1, PRF1, SPP1, CCL8, COL14A1, THY1, ACAN). Among them, POSTN was a significantly important gene. In immunohistochemistry and immunofluorescence, higher POSTN expression was found in areas that co-localize with bronchial epithelial cells of the obliterative bronchiole. In addition, upregulation of POSTN was confirmed in a western blot of another sample dataset of human lung tissue through comparison of donor lung and CLAD lung tissue Conclusion Upregulation of POSTN is a feature of CLAD in lung transplant, mostly located in bronchial epithelial cells of the obliterative bronchiole. POSTN could be a potential target for the pathogenic mechanism of CLAD.

CASE REPORT : Spontaneous Splenic Rupture as a Paradoxical Reaction during Treatment for Splenic Tuberculosis

( Hye Ju Yeo ),( Soo Yong Lee ),( Eunyoung Ahn ),( Eun Jung Kim ),( Dae Gon Rhu ),( Kyoung Un Choi ),( Seung Eun Lee ),( Woo Hyun Cho ),( Doo Soo Jeon ),( Yun Seong Kim ) 대한결핵 및 호흡기학회 2013 Tuberculosis and Respiratory Diseases Vol.75 No.5

This report describes a rare case of a patient with splenic tuberculosis (TB) who developed spontaneous splenic rupture after 10 weeks of antituberculous chemotherapy. The patient responded well to the antituberculous regimen prior to the spontaneous splenic rupture. We considered a paradoxical reaction as a cause of the splenic rupture. The patient underwent splenectomy and continuously received initial antituberculous drugs without change. To the best of our knowledge, this is the first report of spontaneous splenic rupture as a paradoxical reaction to antituberculous chemotherapy in an immunocompetent host with splenic TB.

Gene expression profile in ginsenosides Rb2 treated rats with ventilator induced lung injury

( Hye Ju Yeo ),( Woo Hyun Cho ),( Doosoo Jeon ),( Yunseong Kim ),( Taehwa Kim ),( Dohyung Kim ),( Yun Hak Kim ) 대한결핵 및 호흡기학회 2019 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.127 No.-

Backgrounds: we hypothesized that pretreatment with ginsenosides Rb2 could attenuate ventilator induced lung injury (VILI) and lung inflammation in a rat model. We analyzed gene expression profile of lung tissues in ginsenosides treated rats with VILI. Methods: The ginsenosides Rb2 was administered intraperitoneally 5 hours before ventilation. The rats were categorized as (I) sham group; (II) VILI group; (III) ginsenosides Rb2 group. Wet-todry weight ratio, bronchoalveolar lavage fluid (BALF) protein and cytokines, histologic VILI scores and gene expression profile of lung tissues were investigated. Results: VILI was induced by high tidal volume ventilation of 20ml/Kg for 3 hour, as shown with increased the ratio of wet-to-dry weight,tissue VILI score, BALF malondialdehyde (MDA) and TNF alpha. Ginsenosides Rb2 significantly attenuated these increase of wet-to-dry weight, tissue VILI score and BALF TNF alpha and decrease BALF malondialdehyde (MDA) even though not significant. In ginsenosides Rb2 treated group vs VILI groups vs sham group, 8 genes changed expression significantly. Major alterations were found in the N-ethylmaleimide-sensitive factor (Nsf) and Lipocalin-2 (Lcn2), which could be related with microvascular instability and inflammatory response. Conclusions: Pretreatment with a ginsenosides Rb2 attenuates VILI in a rat model. Nsf and Lcn2 play a central role in pathways identified and may represent interesting targets for VILI treatment/prevention.

Outcomes of Patients on the Lung Transplantation Waitlist in Korea: A Korean Network for Organ Sharing Data Analysis

Yeo Hye Ju,Oh Dong Kyu,Yu Woo Sik,Choi Sun Mi,Jeon Kyeongman,Ha Mihyang,Lee Jin Gu,Cho Woo Hyun,Kim Young Tae 대한의학회 2022 Journal of Korean medical science Vol.37 No.41

Background: The demand for lung transplants continues to increase in Korea, and donor shortages and waitlist mortality are critical issues. This study aimed to evaluate the factors that affect waitlist outcomes from the time of registration for lung transplantation in Korea. Methods: Data were obtained from the Korean Network for Organ Sharing for lung-only registrations between September 7, 2009, and December 31, 2020. Post-registration outcomes were evaluated according to the lung disease category, blood group, and age. Results: Among the 1,671 registered patients, 49.1% had idiopathic pulmonary fibrosis (group C), 37.0% had acute respiratory distress syndrome and other interstitial lung diseases (group D), 7.2% had chronic obstructive pulmonary disease (group A), and 6.6% had primary pulmonary hypertension (group B). Approximately half of the patients (46.1%) were transplanted within 1 year of registration, while 31.8% died without receiving a lung transplant within 1 year of registration. Data from 1,611 patients were used to analyze 1-year postregistration outcomes, which were classified as transplanted (46.1%, n = 743), still awaiting (21.1%, n = 340), removed (0.9%, n = 15), and death on waitlist (31.8%, n = 513). No significant difference was found in the transplantation rate according to the year of registration. However, significant differences occurred between the waitlist mortality rates (P = 0.008) and the still awaiting rates (P = 0.009). The chance of transplantation after listing varies depending on the disease category, blood type, age, and urgency status. Waitlist mortality within 1 year was significantly associated with non-group A disease (hazard ratio [HR], 2.76, P < 0.001), age ≥ 65 years (HR, 1.48, P < 0.001), and status 0 at registration (HR, 2.10, P < 0.001). Conclusion: Waitlist mortality is still higher in Korea than in other countries. Future revisions to the lung allocation system should take into consideration the high waitlist mortality and donor shortages.