Genetic Polymorphism in Corticotropin-releasing Hormone Receptor Type-1 in Preeclamptic Korean Women

Ji Hyae Lim,Shin Young Kim,So Yeon Park,Do Jin Kim,Mi Jin Kim,Hyun Kyong Ahn,Jung Yeol Han,Moon Young Kim,Hyun Young Park,Kwang Soo Lee,Young Ju Kim,Hyun Mee Ryu 대한의학유전학회 2011 대한의학유전학회지 Vol.8 No.2

목적: Corticotropin-releasing hormone receptor type 1은 자간전증과 (CRHR1) 같은 비정상적인 태반의 기능을 가지는 산모에서 감소되어 나타나며, 그것의 발현이나 기능은 유전적으로 영향을 받는다. 이번 연구의 목표는 한국인에서CRHR1 유전자 다형성인 c.33+8199C>T과 자간전증 사이의 연관성을 조사하는 것이었다. 대상 및 방법: CRHR1 유전자 다형성은 SNapShot kit와 ABI Prism 3100 Genetic analyzer를 이용하여 203명의 자간전증 임산부와 211명의 정상 임산부에서 측정되었고, 유전자 다형성과 자간전증 위험도 사이의 연관성을 분석하였다. 결과: CRHR1 유전자 다형성의 유전자형과 대립유전자 빈도는 자간전증 임산부와 정상 임산부 사이에 다르지 않았다. 자간전증 발생 위험도는 분석된 유전자 다형성의 드문 대립 형질(C)을 지닌 이종접합 유전자형(TC)이나 동형접합 유전자형(CC)을 수반하는 그룹에서 증가되지 않았다. CRHR1 유전자의 동형접합 유전자형(CC)을 수반하는 그룹에서 중증 자간전증과 조기 자간전증과 같은 자간전증의 합병증 발병 위험에도 차이가 없었다. 결론: 이 연구는 CRHR1 유전자 다형성인 c.33+8199C>T가 한국인 임신부의 자간전증 발생과 연관이 없음을 나타낸다. Purpose: Placental corticotropin-releasing hormone receptor type 1 (CRHR1) expression is reduced in pregnancies with abnormal placental function such as preeclampsia (PE), and the levels and/or function of CRHR1 are genetically influenced. The aim of this study was to investigate the association between the c.33+8199C>T polymorphism in the CRHR1 gene and PE in a Korean population. Materials and Methods: Using a case-control design, the association between the CRHR1 polymorphism and the risk of PE was investigated in 203 individuals with PE and 211 normotensive controls. Genotypes were determined using a SNapShot kit and an ABI Prism 3100 Genetic analyzer. Results: Genotypes and allele frequencies for the CRHR1 polymorphism did not differ between PE and normotensive pregnancies. The variant T allele was more frequent than the ancestral C allele in both of the groups and was more frequent in the controls than in the cases. In risk analysis for PE, there was not an increased risk of preeclampsia in subjects who were concomitant homozygous rare allele genotypes (CC) (OR, 0.3; P=0.15) or heterozygous rare allele genotypes (TC) (OR, 0.8; P=0.29). There were no differences in the complications of PE such as severity or preterm delivery in patients with the CRHR1 polymorphism. Conclusion: Our findings indicate that the CRHR1 polymorphism was not associated with PE in the present Korean study group.

Effective Method for Extraction of Cell-Free DNA from Maternal Plasma for Non-Invasive First-Trimester Fetal Gender Determination : A Preliminary Study

Ji Hyae Lim,So Yeon Park,Shin Young Kim,Do Jin Kim,Mee Jin Kim,Jae Hyug Yang,Moon Young Kim,Min Hyoung Kim,Ho Won Han,Kyu Hong Choi,Hyun Mee Ryu 대한의학유전학회 2010 대한의학유전학회지 Vol.7 No.1

목적 : 모체 혈장으로부터 가장 효과적으로 세포 유리 DNA(cell free DNA, cf-DNA)를 추출하는 방법을 찾기 위해 우리는 viral DNA 추출 방법과 일반 혈액 DNA 추출 방법을 이용하여 비침습적 임신 초기 태아 성별 확인 결과를 비교하였다. 대상 및 방법 : 임신 초기 44명의 임산부로부터 모여진 모체혈장을 통한 전향적 연구가 구성되었다. Cf-DNA는 viral DNA 추출 방법과 일반 혈액 DNA 추출 방법을 이용하여 각각 추출되었다. 정량 형광-중합효소 연쇄 반응(QF-PCR)을 이용하여 SRY 와 AMXY 유전자를 검출하였다. QF-PCR의 진단 정확도는 최종 분만 기록을 토대로 결정하였다. 결과 : 전체 44명의 여성이 실험에 참여하였지만, 최종 분만기록은 단지 36명의 여성에서 획득하였다. 이들 중 16명은 남아를 20명은 여아를 임신하였다. 두 추출 방법에서 태아 성별의 진단적 정확도는 일반 혈액 DNA 추출 방법에 경우 63.9%(23/26)였으며 viral DNA 추출 방법에 경우 97.2% (35/36)였다. 결론 : QF-PCR을 이용한 비침습적 임신 초기 태아 성별 확인에 있어 viral kit를 사용하는 것이 높은 진단적 정확도를 이끌 수 있을 것으로 사료된다. Purpose: To find the most effective method for extraction of cell-free DNA (cf-DNA) from maternal plasma, we compared a blood DNA extraction system (blood kit) and a viral DNA extraction system(viral kit) for non-invasive first-trimester fetal gender determination. Materials and Methods: A prospective cohort study was conducted with maternal plasma collected from 44 women in the first-trimester of pregnancy. The cf-DNA was extracted from maternal plasma using a blood kit and a viral kit. Quantitative fluorescent-polymerase chain reaction (QF-PCR) was used to detect the SRY gene and AMEL gene. The diagnostic accuracy of the QF-PCR results was determined based on comparison with the final delivery records. Results: A total of 44 women were tested, but the final delivery record was only obtained in 36 cases which included 16 male-bearing and 20 female-bearing pregnancies. For the blood kit and viral kit, the diagnostic accuracies for fetal gender determination were 63.9% (23/36) and 97.2% (35/36), respectively. Conclusion: In non-invasive first-trimester fetal gender determination by QF-PCR, using a viral kit for extraction of cf-DNA may result in a higher diagnostic accuracy.

Effective Method for Extraction of Cell-Free DNA from Maternal Plasma for Non-Invasive First-Trimester Fetal Gender Determination: A Preliminary Study

Lim, Ji-Hyae,Park, So-Yeon,Kim, Shin-Young,Kim, Do-Jin,Kim, Mee-Jin,Yang, Jae-Hyug,Kim, Moon-Young,Kim, Min-Hyoung,Han, Ho-Won,Choi, Kyu-Hong,Ryu, Hyun-Mee Korean Society of Medical Genetics and Genomics 2010 대한의학유전학회지 Vol.7 No.1

목 적: 모체 혈장으로부터 가장 효과적으로 세포 유리 DNA(cell free DNA, cf-DNA)를 추출하는 방법을 찾기 위해 우리는 viral DNA 추출 방법과 일반 혈액DNA 추출 방법을 이용하여 비침습적 임신 초기 태아 성별 확인 결과를 비교하였다. 대상 및 방법: 임신 초기 44명의 임산부로부터 모여진 모체 혈장을 통한 전향적 연구가 구성되었다. Cf-DNA는 viral DNA 추출 방법과 일반 혈액 DNA 추출 방법을 이용하여 각각 추출되었다. 정량 형광-중합효소 연쇄 반응(QF-PCR)을 이용하여SRY 와AMXY 유전자를 검출하였다. QF-PCR의 진단 정확도는 최종 분만 기록을 토대로 결정하였다. 결 과: 전체 44명의 여성이 실험에 참여하였지만, 최종 분만 기록은 단지 36명의 여성에서 획득하였다. 이들 중 16명은 남아를 20명은 여아를 임신하였다. 두 추출 방법에서 태아 성별의 진단적 정확도는 일반 혈액 DNA 추출 방법에 경우 63.9% (23/26)였으며 viral DNA 추출 방법에 경우 97.2% (35/36) 였다. 결 론: QF-PCR을 이용한 비침습적 임신초기 태아 성별 확인에 있어 viral kit를 사용하는 것이 높은 진단적 정확도를 이끌 수 있을 것으로 사료된다. Purpose: To find the most effective method for extraction of cell-free DNA (cf-DNA) from maternal plasma, we compared a blood DNA extraction system (blood kit) and a viral DNA extraction system (viral kit) for non-invasive first-trimester fetal gender determination. Materials and Methods: A prospective cohort study was conducted with maternal plasma collected from 44 women in the first-trimester of pregnancy. The cf-DNA was extracted from maternal plasma using a blood kit and a viral kit. Quantitative fluorescent-polymerase chain reaction (QF-PCR) was used to detect the SRY gene and AMEL gene. The diagnostic accuracy of the QF-PCR results was determined based on comparison with the final delivery records. Results: A total of 44 women were tested, but the final delivery record was only obtained in 36 cases which included 16 male-bearing and 20 female-bearing pregnancies. For the blood kit and viral kit, the diagnostic accuracies for fetal gender determination were 63.9% (23/36) and 97.2% (35/36), respectively. Conclusion: In non-invasive first-trimester fetal gender determination by QF-PCR, using a viral kit for extraction of cf-DNA may result in a higher diagnostic accuracy.

Genetic Polymorphism in Corticotropin-releasing Hormone Receptor Type-1 in Preeclamptic Korean Women

Lim, Ji-Hyae,Kim, Shin-Young,Park, So-Yeon,Kim, Do-Jin,Kim, Mi-Jin,Ahn, Hyun-Kyong,Han, Jung-Yeol,Kim, Moon-Young,Park, Hyun-Young,Lee, Kwang-Soo,Kim, Young-Ju,Ryu, Hyun-Mee Korean Society of Medical Genetics and Genomics 2011 대한의학유전학회지 Vol.8 No.2

목적: Corticotropin-releasing hormone receptor type 1(CRHR1)은 자간전증과 같은 비정상적인 태반의 기능을 가지는 산모에서 감소되어 나타나며, 그것의 발현이나 기능은 유전적으로 영향을 받는다. 이번 연구의 목표는 한국인에서 CRHR1 유전자 다형성인 c.33+8199C>T과 자간전증 사이의 연관성을 조사하는 것이었다. 대상 및 방법: CRHR1 유전자 다형성은 SNapShot kit와 ABI Prism3100 Genetic analyzer를 이용하여 203명의 자간전증 임산부와 211명의 정상 임산부에서 측정되었고, 유전자 다형성과 자간전증 위험도 사이의 연관성을 분석하였다. 결과: CRHR1 유전자 다형성의 유전자형과 대립유전자 빈도는 자간전증 임산부와 정상 임산부 사이에 다르지 않았다. 자간전증 발생 위험도는 분석된 유전자 다형성의 드문 대립 형질(C)을 지닌 이종접합 유전자형(TC)이나 동형접합 유전자형(CC)을 수반하는 그룹에서 증가되지 않았다. CRHR1 유전자의 동형접합 유전자형(CC)을 수반하는 그룹에서 중증 자간전증과 조기 자간전증과 같은 자간전증의 합병증 발병 위험에도 차이가 없었다. 결론: 이 연구는CRHR1 유전자 다형성인 c.33+8199C>T가 한국인 임신부의 자간전증 발생과 연관이 없음을 나타낸다. Purpose: Placental corticotropin-releasing hormone receptor type 1 (CRHR1) expression is reduced in pregnancies with abnormal placental function such as preeclampsia (PE), and the levels and/or function of CRHR1 are genetically influenced. The aim of this study was to investigate the association between the c.33+8199C>T polymorphism in the CRHR1 gene and PE in a Korean population. Materials and Methods: Using a case-control design, the association between the CRHR1 polymorphism and the risk of PE was investigated in 203 individuals with PE and 211 normotensive controls. Genotypes were determined using a SNapShot kit and an ABI Prism 3100 Genetic analyzer. Results: Genotypes and allele frequencies for the CRHR1 polymorphism did not differ between PE and normotensive pregnancies. The variant T allele was more frequent than the ancestral C allele in both of the groups and was more frequent in the controls than in the cases. In risk analysis for PE, there was not an increased risk of preeclampsia in subjects who were concomitant homozygous rare allele genotypes (CC) (OR, 0.3; P=0.15) or heterozygous rare allele genotypes (TC) (OR, 0.8; P=0.29). There were no differences in the complications of PE such as severity or preterm delivery in patients with the CRHR1 polymorphism. Conclusion: Our findings indicate that the CRHR1 polymorphism was not associated with PE in the present Korean study group.

Protein Modifications in Human Diseases(Proteomics) : Peroxisome Proliferator-Activated Receptorγ Agonist Inhibits Adipocyte Differentiation of 3T3-L1 Cells via Activation of ERK

( Jin A Kim ),( Dong Cho Han ),( Jung Min Kim ),( Seung Jun Kim ),( Joo Rang Woo ),( Jo Won Lee ),( Suk Kyeong Jung ),( Hyae Gyeong Cheon ),( Sung Soo Kim ),( Kab Seog Yoon ),( Byoung Mog Kwon ) 한국생화학분자생물학회 (구 한국생화학회) 2008 생화학분자생물학회 춘계학술발표논문집 Vol.2008 No.-

Effects of sevoflurane on metalloproteinase and natural killer group 2, member D (NKG2D) ligand expression and natural killer cell-mediated cytotoxicity in breast cancer: an in vitro study

Kim Hyae Jin,Jeon Soeun,Lee Hyeon-Jeong,Bae Jaeho,Ri Hyun-Su,홍정민,Paek Sung In,Kwon Seul Ki,Kim Jae-Rin,Park Seungbin,Yun Eun-Jung 대한마취통증의학회 2023 Korean Journal of Anesthesiology Vol.76 No.6

Background: We investigated the effects of sevoflurane exposure on the expression of matrix metalloproteinase (MMP), expression and ablation of natural killer group 2, member D (NKG2D) ligands (UL16-binding proteins 1–3 and major histocompatibility complex class I chain-related molecules A/B), and natural killer (NK) cell-mediated cytotoxicity in breast cancer cells.Methods: Three human breast cancer cell lines (MCF-7, MDA-MB-453, and HCC-70) were incubated with 0 (control), 600 (S6), or 1200 μM (S12) sevoflurane for 4 h. The gene expression of NKG2D ligands and their protein expression on cancer cell surfaces were measured using multiplex polymerase chain reaction (PCR) and flow cytometry, respectively. Protein expression of MMP-1 and -2 and the concentration of soluble NKG2D ligands were analyzed using western blotting and enzyme-linked immunosorbent assays, respectively. Results: Sevoflurane downregulated the mRNA and protein expression of the NKG2D ligand in a dose-dependent manner in MCF-7, MDA-MB-453, and HCC-70 cells but did not affect the expression of MMP-1 or -2 or the concentration of soluble NKG2D ligands in the MCF-7, MDA-MB-453, and HCC-70 cells. Sevoflurane attenuated NK cell-mediated cancer cell lysis in a dose-dependent manner in MCF-7, MDA-MB-453, and HCC-70 cells (P = 0.040, P = 0.040, and P = 0.040, respectively).Conclusions: Our results demonstrate that sevoflurane exposure attenuates NK cell-mediated cytotoxicity in breast cancer cells in a dose-dependent manner. This could be attributed to a sevoflurane-induced decrease in the transcription of NKG2D ligands rather than sevoflurane-induced changes in MMP expression and their proteolytic activity.

Metastasis Within the Stomach From Esophageal Cancer After Surgery: Computed Tomography Findings in 6 Patients

Kim, Hyae Young,Lee, Jong Seok,Chae, Eun Jin,Lee, Geon Kook,Kim, Moon Soo,Zo, Jae-il Lippincott Williams Wilkins, Inc. 2009 Journal of computer assisted tomography Vol.33 No.1

PURPOSE:: We describe clinical and computed tomography (CT) features of intrathoracic stomach tube metastasis from esophageal cancer after surgical resection found on follow-up chest CT scans. METHODS:: Medical records and postoperative CT scans were reviewed in 6 patients who had a confirmed intrathoracic stomach tube metastasis after esophageal cancer operation. RESULTS:: All patients died 1 to 13 months (mean [SD], 5.8 [4.9] months) after the diagnosis of stomach metastasis. Computed tomography scans revealed eccentric stomach wall thickening with abnormal enhancement (n = 3) or an intramural mass with apparent intact mucosa (n = 3). Eccentric wall thickening with abnormal enhancement was located in the upper portion of the stomach tube (n = 3), and intramural mass in the lower (n = 3) with reference to the carina level. Mediastinal lymph node (9 and 10 mm, respectively) was noted in 2 patients. CONCLUSIONS:: The patients with stomach metastasis from esophageal cancer had poor prognosis. During postoperative evaluation of patients with surgically resected esophageal cancer, stomach metastasis from esophageal cancer seemed as an eccentric wall thickening with abnormal enhancement or an intramural tumor with apparent intact mucosa on CT.

Lung Cancer Screening with Low-Dose CT in Female Never Smokers: Retrospective Cohort Study with Long-term National Data Follow-up

Kim, Hyae Young,Jung, Kyu-Won,Lim, Kun Young,Lee, Soo-Hyun,Jun, Jae Kwan,Kim, Jeongseon,Hwangbo, Bin,Lee, Jin Soo Korean Cancer Association 2018 Cancer Research and Treatment Vol.50 No.3

<P><B>Purpose</B></P><P>Because of growing concerns about lung cancer in female never smokers, chest low-dose computed tomography (LDCT) screening is often performed although it has never shown clinical benefits. We examinewhether or not female never smokers really need annual LDCT screening when the initial LDCT showed negative findings.</P><P><B>Materials and Methods</B></P><P>This retrospective cohort study included 4,365 female never smokers aged 40 to 79 years who performed initial LDCT from Aug 2002 to Dec 2007. Lung cancer diagnosis was identified from the Korea Central Cancer Registry Database registered until December 31, 2013. We calculated the incidence, cumulative probability, and standardized incidence ratio (SIR) of lung cancer by Lung Imaging Reporting and Data System (Lung-RADS) categories showed on initial LDCT.</P><P><B>Results</B></P><P>After median follow-up of 9.69 years, 22 (0.5%) had lung cancer. Lung cancer incidence for Lung-RADS category 4 was 1,848.4 (95% confidence interval [CI], 1,132.4 to 3,017.2) per 100,000 person-years and 16.4 (95% CI, 7.4 to 36.4) for categories 1, 2, and 3 combined. The cumulative probability of lung cancer for category 4 was 10.6% at 5 years and 14.8% at 10 years while they were 0.07% and 0.17% when categories 1, 2, and 3 were combined. The SIR for subjects with category 4 was 43.80 (95% CI, 25.03 to 71.14), which was much higher than 0.47 (95% CI, 0.17 to 1.02) for categories 1, 2, and 3 combined.</P><P><B>Conclusion</B></P><P>Considering the low risk of lung cancer development in female never smokers, it seems unnecessary to repeat annual LDCT screening for at least 5 years or even longer unless the initial LDCT showed Lung-RADS category 4 findings.</P>

Association of the 252A>G and 804C>A Polymorphisms of the Lymphotoxin-α Gene with Coronary Artery Disease in Korean Men

( Hyae Jin Kim ),( Yang Soo Jang ),( Jey Sook Chae ),( Soo Jeong Koh ),( Oh Yoen Kim ),( Yae Jung Hyun ),( So Yeon Choi ),( Sung Ha Park ),( Sang Hak Lee ),( Yu Mi Heo ),( Hong Keun Cho ),( Jong Ho Le 한국지질동맥경화학회(구 한국지질학회) 2006 한국지질동맥경화학회 추계학술발표논문집 Vol.2006 No.-

Dose-volumetric Parameters for Predicting Severe Radiation Pneumonitis after Three-dimensional Conformal Radiation Therapy for Lung Cancer

Kim, Tae Hyun,Cho, Kwan Ho,Pyo, Hong Ryull,Lee, Jin Soo,Zo, Jae Ill,Lee, Dae Ho,Lee, Jong Mog,Kim, Hyae Young,Hwangbo, Bin,Park, Sung Yong,Kim, Joo Young,Shin, Kyung Hwan,Kim, Dae Yong Radiological Society of North America 2005 Radiology Vol.235 No.1

<P>PURPOSE: To retrospectively evaluate dose-volumetric parameters for association with risk of severe (grade >/=3) radiation pneumonitis (RP) in patients after three-dimensional (3D) conformal radiation therapy for lung cancer. MATERIALS AND METHODS: The study was approved by the institutional review board, which did not require informed consent. Data from 76 patients (66 men, 10 women; median age, 60 years; range, 35-79 years) with histologically proved lung cancer treated curatively with 3D conformal radiation therapy between August 2001 and October 2002 were retrospectively analyzed. Twenty patients underwent surgery before radiation therapy; 57 patients received chemotherapy. Median total radiation dose of 60 Gy (range, 54-66 Gy) was delivered in 30 (range, 27-33) fractions over 6 weeks. RP was scored by using Radiation Therapy Oncology Group criteria. Clinical parameters were analyzed. Dose-volumetric parameters analyzed were percentage of lung volume that received a dose of 20 Gy or more (V20), 30 Gy or more (V30), 40 Gy or more (V40), or 50 Gy or more (V50); mean lung dose (MLD); normal tissue complication probability (NTCP); and total dose. Fisher exact test was performed to compare clinical parameters between patients who developed severe RP and those who did not. Univariate and multivariate logistic regression analyses were performed to evaluate data for association between dose-volumetric parameters and severe RP. Pearson chi(2) test was used to assess data for correlations among dose-volumetric parameters. P < or = .05 was considered to indicate statistically significant difference. RESULTS: Of 76 patients, 30 (39%) did not develop RP; 23 (30%) developed RP of grade 1; 11 (14%), grade 2; 11 (14%), grade 3; and 1 (1%), grade 4. None had grade 5 RP. Age (< 60 vs > or =60), sex, Karnofsky performance status (< 70 vs > or =70), forced expiratory volume in 1 second, presence of weight loss, preexisting lung disease, history of thoracic surgery, and history of chemotherapy did not significantly differ between patients who developed severe RP and those who did not. In univariate analyses, MLD, V20, V30, V40, V50, and NTCP were associated with severe RP (P < .05). In multivariate analysis, MLD was the only variable associated with severe RP. CONCLUSION: MLD is a useful indicator of risk for development of severe RP after 3D conformal radiation therapy in patients with lung cancer.</P>