65세 이상의 노령층에서 고지혈증의 위험요인

김용배,장원기,황보영,김화성,안규동,이병국,이용진,김세훈,김승수,김승훈,김영록,김웅빈,이성수 순천향의학연구소 2006 Journal of Soonchunhyang Medical Science Vol.12 No.1

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연쇄중합효소반응을 이용한 태아성별진단

이희섭,김용신,김화선,김정중,김원신 원광대학교 생명공학연구소 1996 생명공학연구소보 Vol.4 No.1

For sex determination by the PCR method, oligoprimers to Y-chromosome gene, DYZ1, SRY, and AMGL were synthesized and genomic DNA was extracted from male and female placenta for the control use. DYZ1 represented 154 bp single band to 0.001pg/ml male genomic DNA but did not represent 154 bp band in female genomic DNA, SRY represented 341 bp band to 1 pg/ml male genomic DNA but did not represent 341 bp band in female genomic DNA, and AMGL represented 977 and 788 bp double band to 1 pg/ml male genomic DNA and 977 bp single band to 1 pg/ml female genomic DNA in 2% agarose gel electrophoresis stained with ethidium bromide. DYZ1 was 1,000-fold sensitive than SRY and AMGL. DYZ1 and SRY could not identify the PCR failure from female but AMGL identified. To increase the sensitivity, the dual amplification of AMGL was performed and the sensitivity increased to 1,000-fold. During the dual amplification of female genomic DNA mixed with male genomic DNA, 0.00125pg/l, 1:400 part male genomic DNA contamination represented double bands as male. In 2 cases of 46, XY female, DYZ1 and AMGL amplification represented male band but SRY amplification did not represent male band. It was suggested that SRY gene was deleted in two 46, XY female cases. for fetal sex determination, PCR with DYZ1, SRY, and AMGL was performed in 10 cases of chorionic villi and 15 cases of amnionic cells. By the comparison with karyotyping result, fetal sex determination was achieved successfully in all 23 samples using PCR of SRY and AMGL but false result was detected in 3 cases(13%) using DYZ1. According to our results, it was concluded that DYZ1 was 1,000-fold sensitive than SRY and AMGL but could not be used because of its false results, and AMGL and SRY must be used concomitantly for precise sex determination.

경련성 환아 부모들의 인식에 대한 연구

이경화(Kyoung Hwa Lee),서은숙(Eun Sook Suh),김영창(Yong Chang Kim),김창휘(Chang Hwi Kim),이동환(Dong Whan Lee) 대한소아신경학회 1996 대한소아신경학회지 Vol.3 No.2

Convulsive disorder is the most part of the pediatric neurologic disease. As far as the treatment of convulsive disorder concerned, we must consider familys' understanding, and the close cooperation between the epileptic child, parents, and doctors. We compared to Kim's report in 1985, to study the change of parents of epileptic children thinking. A prospective study was undertaken the analysis of 100 epileptic children parents visited to the pediatric dept, of Soonchunhyang university hospital from May to July, 1995. Psychological assessment, questionnaires which contains 17 items were given them, and these items were compared to Kims report in 1985 The results were as follows: 1) Parents of epileptic children answered convulsive disorder has influenced to children growth and development( 60.8% in 1985, 65.0% in 1995 ). 2) For asking the causes of children disease, 40.5% of them in 1985 and 50.0% of them in 1995 answered to uncertain, but only 7.8% of them in 1985 and 7.0% of them in 1995 answered to relation with familial inheritance. 3) The feelings at time of the diagnosis were gloomy or uncomfortable: 52.3% in 1985, 38.0% in 1995 and shameful 28.1% in 1985, 9.0% 1995. parents had belived in the epilepsy was treatable disease: 41.0% in 1995. 4) According to the this survey, patients explained reasons why their children take the medication increased from 23.5% in 1985 to 65.0% in 1995. 5) Only family, knew that their children had the convulsive disorder about 50.3% in 1995 and 22.0% in 1995. Family, patients and other people except family knew that their children had the convulsive disorder about 6.5% in 1985 and 43.0% in 1995. 6) The parents opinion and expectation toward the medical doctors were that they wanted to get full explanation for the disease 81.0% in 1985 and 35.1% in 1995, and others were explantation about toxicity and side effects of the medication and advises for the childrens life and activities.

납 노출 근로자들에서 ALAD 및 VDR의 다형질성이 조혈기능 지표에 미치는 영향

이성수,김남수,김진호,김용배,황보영,김화성,안규동,이병국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Objectives: This study was conducted with new workers who entered lead industries from 1992 to 2001 to evaluate the genetic susceptibility of ALAD (δ-aminolevulinic acid dehydratase) and VDR (vitamin D receptor) gene on health effect of lead exposure. Methods: Among the subjects of the database of lead industries at the Soonchunhyang University Institute of Industrial Medicine, only new workers were selected for this study. The total of eligible workers for this category was 3,540 workers, including non lead exposed workers of same lead industries. From stored blood in specimen bank of Soonchunhyang University, genotype of ALAD and VDR were measured using PCR method. Variables for this study were blood lead as an index of lead exposure, ZPP (zinc protoporphyrin in blood), urine ALA (δ-aminolevulinic acid), and hemoglobin as an index of hematopoietic effect of lead. Information on sex, job duration, and weight were collected for personal information. The data were analyzed using SAS (version 8.2) with descriptive analysis of t-test and multiple regression analysis. Results: Among 3,540 new employed study subjects during period of 1992-2001, 3,204 workers (90.5%) had ALAD genotype 1-1, while 336 workers (9.5%) had variant type of ALAD (1-2 or 2-2). For VDR genotype, 2,903 workers (89.7%) out of total tested 3,238 workers were belonged to type bb and 335 workers (10.5%) were type bB or BB. The distribution of genotype of ALAD and VDR were not different according to the job duration in male workers, but were different in female workers. The effect of ALAD and VDR genotype on blood lead were positively significant in the analysis of all cumulative data of new employed workers for 10 years. The effect of VDR genotype on blood lead were stronger than that of ALAD While the variant ALAD gene made decrease of mean ZPP and ALA in urine after controlling for blood lead and other covariate, the variant VDR gene made increased the mean ZPP and ALA in urine in all cumulative data analysis and cross sectional analysis by job duration. For hemoglobin, ALAD and VDR genotype did not affect the mean value. Conclusions: From the above our results, we found that ALAD and VDR genotype exerted significant effect in various way. We confirmed that the finding of a cross sectional study of protective effect of variant ALAD on the effect blood lead on blood ZPP in our retrospective study design. It was found that VDR did not exert protective effect for lead exposure as the variant ALAD did.

축산분뇨 혐기소화 잔재물의 특성 및 초지 적용성 평가

김승민(Seung Min Kim),정웅기(Woong-gi Jung),성용주(Yong Joo Sung),안희권(Hee-Kwon Ahn),김동성(Dong Sung Kim),윤도현(Do-Hyun Yoon),김동섭(Dong-Seop Kim1),정광화(Hwa Gwang Jung) 한국펄프·종이공학회 2014 펄프.종이기술 Vol.46 No.3

전상회계 전자교재(e-Textbook) 개발에 관한 연구

김소형,정용화 한국직업능력개발원 2003 직업능력개발연구 Vol.6 No.1

This study researched to apply a accounting education method of the future that to consider college environment. The research model was completed to be analysis a concept, strong-weakness of e-Book & e-Text Book, environment factor for development of accounting e-textbook. The contents development used to the effect of animation and made the simple design. It was to be purposed to study an important accounting knowledge fast. The accounting e-textbook was made to use the PC and internet explore for convenient the students. This method learn anywhere the computer accounting program.

연취급 근로자들의 사회 심리적 스트레스가 연노출 수준에 미치는 영향

김용배,황보영,김화성,이성수,안규동,이병국 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.1

Objectives : This study was carried out to assess psychosocial distress of lead workers and to examine the effect of psychosocial distress on lead exposure indices. Methods : The study design was cross-sectional and research subjects were 246 lead workers in primary smelting industry. General characteristics, job characteristics and social support were obtained by self-administered questionnaire and history taking. Psychosocial distress was measured using PWI(Psycosocial Well-being Index), a 45-item self-administered instrument. Blood lead(PbB), zinc protoporphyrin(ZPP), δ-aminolevulinic acid in urine(ALAU) were selected as indicators of lead exposure. Results : The crude odds ratio of group whose PbB was 20-39 ㎍/㎗ was 3.475(95% CI : 1.243-6.404) and that of group whose PbB was above 40 ㎍/㎗ was 7.586(95% CI : 1.487-15.611). The odds ratio of each group adjusted age, wages, job characteristics, social support, smoking and alcohol drinking habit were still significant. Conclusions : Our results supported the association between lead exposure and psychosocial distress. The inclusion of psychosocial distress measurement as a special health examination for lead workers is highly recommended for the effective prevention of stress in lead workers.

製造企業의 經營環境에 관한 硏究 : 김해지역을 중심으로

金東沃,孫炳基,金榮九,李道和,長英壹,崔容銑 인제대학교 1994 仁濟論叢 Vol.10 No.2

At the threshold of localization era, there is a great need to determine local economic development agenda. But current paucity of data regarding local firms' operations can be a hindering factor to achieve above objective. This study aims to provide such a data which can be used for setting local economic policy of Kimhae area. The study was conducted by administering survey questionnaires. The sample is 34 small sized manufacturers with less than 300 employees in Kimhae area. The content of survey includes General Management, Personnel and Labor Relations, Marketing, Production, Finance, Accounting and MIS. The results of survey revealed that current managerial practices in general showed sizable gap with desired one, which is mandated by national goal of reinforcement of competitiveness of small and medium sized firms.

보존료가 두부의 저장에 미치는 효과

김동한,신용서,전승호,백승화,이갑상 圓光大學校 1992 論文集 Vol.26 No.2

두부의 저장성 향상을 위해 보존료를 첨가하여 저장중 두부의 미생물상과 이화학적 변화를 검토하였다. 보존료 첨가에 의한 두부의 기호도 변화는 큰 차이가 없었으며 부패가 진행될 수록 적정산도와 amino태 및 ammonia태 질소량은 증가하였다. 두부는 acetic acid로 응고시키는 것이 CaCl₂보다 보존료에 의한 저장성 향상에 효과적이어서, acetic acid에 0.05% K-sorbate와 3% NaCl을 혼합첨가한 구는 저장중 이화학적 변화 및 호기성, 혐기성 세균수가 ?? cell/g수준인 점을 고려해 볼 때 30℃에서 48시간까지 저장이 가능하였다. In order to improve the shelf life of Tofu, the effects of preservatives on Tofu were investigated for microbiol and physicochemical changes during its storage. The sensory evaluation was not deteriorated by preservatives (0.05% K-sorbates). According to the progression of spoliage of Tofu, titratable acidity, and amino and ammonia nitrogen contents were increased. The effect of chemical preservatives on the shelf life of Tofu was shown to be stronger in the Tofu by acetic acid than in that by CaCl₂. The presence of 0. 05% K-sorbate and 3% NaCl as a preservative in Tofu by acetic acid extended it's shelf life upto 48 hrs at 30℃.

살충제 Carbofuran의 수중광분해

김균,김용화 한국환경농학회 2002 한국환경농학회지 Vol.21 No.3

살충제 carbofuran의 수중 광분해 실험을 미국 EPA 및 OECD 방법으로 수행하였다. carbofuran은 자연광을 흡수하지 않는 화합물이지만, 야외에서 증류수로 수행한 광분해시험결과 반감기가 9.7일, SUNTEST에서 3.4일, SUNTEST의 UV하에서 1시간으로 신속한 광분해가 일어남을 확인하였다. 특히 논물에서는 SUNTEST에서의 반감기가 14시간으로 증류수보다 약 6배 가속화되었으며, 살균한 논물보다는 비살균 논물에서의 광분해가 다소 빠르게 일어났다. 이와 같은 결과로 볼때 carbofuran의 경우 수도작 상황에서 seneitizer 등에 의한 간접 광분해의 가능성이 있음을 확인하였다. Photodegradation rates of carbofuran in aqueous solution were measured under various test conditions mainly following the guidelines of U.S. EPA and OECD. Half-lives of carbofuran in distilled water were 9.7 days, 3.3 days, and 1 hr under natural sunlight, SUNTEST with UV filter, and SUNTEST without UV filter, respectively. Waters from a paddy field accelerated the decomposition by factor of 6. It was confirmed that the use of SUNTEST could shorten the test period of photodegradation.