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A medium-maturing, disease resistant and good-quality rice variety Geumanbyeo
Hung-goo Hwang,Yeon-gyu Kim,Ha-cheol Hong,Yong-hwan Choi,Man-kee Baek,Im-soo Choi,Young-chan Cho,Chang-in Yang,Hong-yeol Kim,Jong-doo Yea,Young-seop Shin,Kyung-ho Kang,Sang-nag Ahn 한국육종학회 2007 한국육종학회지 Vol.39 No.4
“Geumanbyeo” is a new japonica rice variety developed by the rice breeding team of National Institute of Crop Science, RDA in 2002, which was derived from a cross between SR11878-14-4-1 of good eating-quality and high yield potential, and Suweon345 of lodging tolerance and resistance to diseases. Geumanbyeo is a medium-maturing variety with heading date of August 12 on ordinary planting in the middle plain area. It has a semi-erect plant type with culm length of 82cm, erect pubescent leaf blade and slightly tough culm with good canopy architecture. Geumanbyeo has a little bit fewer tillers per hill, and more spikelets per panicle than Hwaseongbyeo. It is slightly less tolerant to the cold stress in terms of both heading delay and spikelet fertility than Hwaseongbyeo. This variety shows delayed leaf senescence and considerable tolerance to viviparous germination at ripening stage. It shows moderately resistant to leaf blast and K1 race of bacterial blight, but susceptible to virus diseases and insect pests. The milled rice of Geumanbyeo exhibits translucent, relatively clear non-glutinous endosperm and medium-short grain. It showed lower amylose content of 18.6% and similar gelatinization temperature and better palatability of cooked rice compared to Hwaseongbyeo. The milled rice yield of this variety is about 5.32 MT/ha at ordinary culture in local adaptability test for three years. Geumanbyeo would be highly adaptable to the central lowland and mid-southern mountainous areas in Korea. “Geumanbyeo” is a new japonica rice variety developed by the rice breeding team of National Institute of Crop Science, RDA in 2002, which was derived from a cross between SR11878-14-4-1 of good eating-quality and high yield potential, and Suweon345 of lodging tolerance and resistance to diseases. Geumanbyeo is a medium-maturing variety with heading date of August 12 on ordinary planting in the middle plain area. It has a semi-erect plant type with culm length of 82cm, erect pubescent leaf blade and slightly tough culm with good canopy architecture. Geumanbyeo has a little bit fewer tillers per hill, and more spikelets per panicle than Hwaseongbyeo. It is slightly less tolerant to the cold stress in terms of both heading delay and spikelet fertility than Hwaseongbyeo. This variety shows delayed leaf senescence and considerable tolerance to viviparous germination at ripening stage. It shows moderately resistant to leaf blast and K1 race of bacterial blight, but susceptible to virus diseases and insect pests. The milled rice of Geumanbyeo exhibits translucent, relatively clear non-glutinous endosperm and medium-short grain. It showed lower amylose content of 18.6% and similar gelatinization temperature and better palatability of cooked rice compared to Hwaseongbyeo. The milled rice yield of this variety is about 5.32 MT/ha at ordinary culture in local adaptability test for three years. Geumanbyeo would be highly adaptable to the central lowland and mid-southern mountainous areas in Korea.
Evaluation of Iron and Zinc Content in Rice Germplasms
Jeom-Ho Lee,Kyu-Seong Lee,Hung-Goo Hwang,Chang-Ihn Yang,Sang-Bok Lee,Young-Hwan Choi,O-Young Jeong,Parminder Virk 한국육종학회 2008 한국육종학회지 Vol.40 No.2
The germplasm of 246 rice cultivars was analysed for iron and zinc contents using a Inductively Coupled Argon Plasma (ICP) at International Rice Research Institute (IRRI) Philippines. Iron contents ranged from 2.0 to 12.0, and zinc ranged from 10.0 to 33.0 (mg/kg), showing with the mean values of 4.3 and 22.8 (mg/kg), respectively. In genotypes tested, there was approximately a two-fold difference in iron and zinc concentrations, suggesting a genetic potential to increase these micronutrients in rice grain. A highly significant positive correlation (r2=0.503) was found between iron and zinc contents. Iron contents decreased drastically as polishing time increased, whereas zinc decreased only slightly. In the interaction between genotype and environment on iron contents, genotype (G), environment (E), and the G × E interactions accounted for 69%, 5% and 26% of the sums of squares, respectively. Indicating that genotype is would be the most significant factor for the to improve iron contents of rice in rice breeding, suggesting that therefore identifying genotypes with relatively stable performance across various environments is important as staple food crops.
Presenilin-2 유전자 이식에 의한 alzheimer 질환모델동물의 개발
황대연,채갑용,신동환,황진희,임채형,김연주,김범진,구준서,김용규,조정식 식품의약품안전청 2000 식품의약품안전청 연보 Vol.4 No.-
알츠하이머는 신경세포에 Aβ-42의 침적으로 신경원섬운 농축이나 시냅스 상실 풍이 발생하여 나타나는 신경퇴행성 질환이다. 치매의 원인 단백질인 Aβ는 APP가 secreatasr에 절단되어 형성되며 APP내에 돌연변이는 퍽상적인 Aβ-40보다 Aβ-42의 비정상적인 형태를 더욱 많이 형성하여 치매를 유발하는 것으로 보고피었다. 이와 더불어 60세 "1전에 발생하는 조기유발성 치매는 presenilin이라는 단백질에 의해서 촉진되며, 특히 PS-2가 중요한 유발원으로 생각피고 았다. PS-2는 Aβ-42의 생성을 촉진시킬 뿐만 아니라 apoptosls동안에 caspase에의해 정상과 다른형태로 절단되었다. 그러나 PS-2가 정확히 어떠한 기작에 의해서 Af의 생성을 촉진시키고 apoptosis에 관여하는지는 밝혀져 있지 않다. 따라서 본 연구에서는 조기유발성 치매의 원인 단백질을 이용한 인간화모델동물을 생산하핀 이를 이용하여 치매의 발생기작을 연구하며 치매 치료제의 유효성과 안전성을 평가하는데 기여하고자한다. 당해 연로에는 정상 마우스치 각 조직에서 PS-2 유전자의 발현을 관찰하여 다양한 조직에서 PS-2가 발현 됨을 확인하였다. 또한 순부분리된 재조합 유전자를 마우스 수정란에 주입하여 3마리의 pTet-tTAK 유전자가 삽입된 형질전환 마우스를 개발하였고 pTet-wild PS-2 마우스 1마리와 pTet-N1411 PS-2 마우스 3마리를 생산하였다. 확인된 마우스는 double transgenic 마우스를 생산하기 위차여 교배에 와해 4마리의 doubftransgenic 마우스를 생산하였다. 현재 개발된 마우스를 이용하여 tetracycline 발현 온도를 실시중이며 다른 종류의 형질전환 마우스를 개발 중에 있다. A1zheimer's Disease (AD) occurs when neurons in memory and cognition region of brain are damaged and u14imately killed. A key step in this process Is the polymeriTation ofthe A β -peptide into neurotoxic protein fillaments and these filaments are accumulated in thecerebral cortex and hippocamTlus of AD as the extracrllular senile plaq·ues that are composed ofthe 40 to 42/3 amino acid long peptide. Aβ fragment derived from amyloid precusor protein(fIPP) and intracellular neurc,fibrillary tangles are composed of twisted filament of tau protein.Since there is a Back of basi,c mechanisms of AD for which no animal is available, underlyingcauses of AD remain obscure and therapeutic development is progressing slowly. Transgenictechnology have provided an uniaue opportunity to study of how gene is associated with thehuman cognitive dysfunction after synaptic transrrdssion between neurons in the brain. Genesfor presenilins (PSs) are kno·Pn to be involved in the development of AD and are accounts forcase of early-onset of AD leading to the development of AD in patient younger than 60 years.It is interesting that overexpression of PS-2 witd type in PCl2 cells enhances the apoptosisinduced by A β and mutant PS-2 increases apoptotic activiry when compared with PS-2 wildtype. In this study, Human rTild and Nlrtll PS-2 gene were inserted into pTet-splice under tr,econtrol of tetracycline operaf:or This pTe?-PS-2 was injected into mouse fertilized embryo.Three transgenic mice carrying pTet-tTAK plasmid, one transgenic mice carrying pTet-wildPS-2 plasmid and three transgenic mice carrying pTet-N1411 PS-2 plasmid were generated. Inp'ret-tTAK transgenic ndce, the expresslon of transactivator increase with dose of tetracycline.Thus, modei animals mated from each Tet-PS-2M and Tet-tTAK transgenic animal will beuseful for eTarγline the mechanisms and drug screen.
A Medium-maturing, Multi-Disease Resistant and High-Quality Rice Cultivar, "Sampyeongbyeo"
Hae Chune Choi,Hung Goo Hwang,Ha Cheol Hong,Yeon Gyu Kim,Chang In Yang,Young Chan Cho,Yong Hwan Choi,Sae Jun Yang,Hong Yeol Kim,Jong Doo Yea,Huhn Pal Moon,Soo Yeon Cho,Sang Nag Ahn 한국육종학회 2005 한국육종학회지 Vol.37 No.2