Impact of Chronic Lateral Ankle Instability with Lateral Collateral Ligament Injuries on Biochemical Alterations in the Cartilage of the Subtalar and Midtarsal Joints Based on MRI T2 Mapping

Tao Hongyue,Hu Yiwen,Lu Rong,Zhang Yuyang,Xie Yuxue,Chen Tianwu,Chen Shuang 대한영상의학회 2021 Korean Journal of Radiology Vol.22 No.3

Objective: To quantitatively assess biochemical alterations in the cartilage of the subtalar and midtarsal joints in chronic lateral ankle instability (CLAI) patients with isolated anterior talofibular ligament (ATFL) injuries and combined calcaneofibular ligament (CFL) injuries using MRI T2 mapping. Materials and Methods: This study was performed according to regulations of the Committee for Human Research at our institution, and written informed consent was obtained from all participants. Forty CLAI patients (26 with isolated ATFL injuries and 14 with combined ATFL and CFL injuries) and 25 healthy subjects were recruited for this study. All participants underwent MRI scans with T2 mapping. Patients were assessed with the American Orthopedic Foot and Ankle Society (AOFAS) rating system. The subtalar and midtarsal joints were segmented into 14 cartilage subregions. The T2 value of each subregion was measured from T2 mapping images. Data were analyzed with ANOVA, the Student’s t test, and Pearson’s correlation coefficient. Results: T2 values of most subregions of the subtalar joint and the calcaneal facet of the calcaneocuboid joint in CLAI patients with combined CFL injuries were higher than those in healthy controls (all p < 0.05). However, there were no significant differences in T2 values in subtalar and midtarsal joints between patients with isolated ATFL injuries and healthy controls (all p > 0.05). Moreover, T2 values of the medial talar subregions of the posterior subtalar joint in patients with combined CFL injuries showed negative correlations with the AOFAS scores (r = -0.687, p = 0.007; r = -0.609, p = 0.021, respectively). Conclusion: CLAI with combined CFL injuries can lead to cartilage degeneration in subtalar and calcaneocuboid joints, while an isolated ATFL injury might not have a significant impact on the cartilage in these joints.

In-situ fabrication of hollow BiOIxCly n-n type heterojunction microspheres with enhanced visible-light-driven performance for rhodamine B degradation and CO2 reduction

Hongyu Fu,Tao Zhang,Abulikemu Abulizi,Kenji Okitsu,Yalkunjan Tursun 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.117 No.-

It is pressing to explore highly efficient and broad-spectrum photocatalysts to address environmentalissues. In this work, in situ self-assembled hollow BiOIxCly n-n type heterojunction microspheres weresimply synthesized via a one-step hydrothermal method, and the microstructure, morphology, optical,photoelectrochemical, and photocatalytic properties of the samples were researched. The photocatalyticactivity of materials was investigated for Rhodamine B (RhB) degradation and CO2 reduction. The characterizationresults illustrated that the introduction of moderate amounts of Cl in BiOI could promotethe conversion of RhB and CO2, and it improved separation efficiency of photogenerated electrons andholes in BiOI. Specially, when the doping ratio of I and Cl was 2:1, BiOIxCly exhibited the most outstandingphotocatalytic activity for RhB degradation (99.42 %, 20 min) and reduction of CO2 to methanol(1042.12 lmol/gcat, 8 h) and ethanol (264.53 lmol/gcat, 8 h), which were approximately 3.40 and 3.91-times larger than that of BiOI, respectively. This study could describe a potential strategy for assistingwith environmental purification and energy transformation.

Fabrication and properties of reaction-bonded SiC prepared by gelcasting

Lifang Nie,Hongyu Gong,Tao Zhang,Yujun Zhang 한양대학교 세라믹연구소 2009 Journal of Ceramic Processing Research Vol.10 No.1

The properties of green and reaction-bonded SiC (RBSC) samples prepared by a gelcasting technique are investigated in this paper. The results show that solid loading and monomer content of the suspension in the gelcasting process are the main factors that effect the density and shrinkage of the green bodies. A slurry with a solid loading of 58 vol% was solidified in situ to a green body with a linear shrinkage of 1.1% and flexural strength of 27 ± 2 MPa. SEM micrographs show that the SiC and C particles in the green body were closely compact by a connection of the polymer network. The maximum density and flexural strength of samples reaction sintered at 1700℃ in vacuum were 2.97 g/㎤ and 378 ± 12 MPa respectively. The properties of green and reaction-bonded SiC (RBSC) samples prepared by a gelcasting technique are investigated in this paper. The results show that solid loading and monomer content of the suspension in the gelcasting process are the main factors that effect the density and shrinkage of the green bodies. A slurry with a solid loading of 58 vol% was solidified in situ to a green body with a linear shrinkage of 1.1% and flexural strength of 27 ± 2 MPa. SEM micrographs show that the SiC and C particles in the green body were closely compact by a connection of the polymer network. The maximum density and flexural strength of samples reaction sintered at 1700℃ in vacuum were 2.97 g/㎤ and 378 ± 12 MPa respectively.

Functional characterization of SlGSTD3 and SlGSTD4 associated with phoxim and chlorpyrifos resistance in Spodoptera litura

Xu Li,Liu Hongyu,Yang Tao,He Chengshuai,Li Bo,Song Genmiao,Zhou Lin,Liu Runqiang 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.4

Glutathione S-transferases (GSTs) is one of the main detoxification enzyme systems in insects and play important roles in insecticide resistance by direct metabolism, sequestration and antioxidant activity. Several GSTs genes in Spodoptera litura, a polyphagous agricultural pest, have been demonstrated to be overexpressed and involved in organophosphates and pyrethroids resistance. Previous studies have indicated the significant overexpression of two delta class GSTs genes (SlGSTd3 and SlGSTd4) in organophosphates and pyrethroids resistant populations. Here, they were heterologous expressed, and their metabolism activity and antioxidant activity were determined. Results indicated that the recombinant protein SlGSTD3 and SlGSTD4 both showed metabolism activity to phoxim and chlorpyrifos, but not to fenvalerate, cyhalothrin or beta cypermethrin. The metabolism activity of SlGSTD3 to phoxim and chlorpyrifos is higher than that of SlGSTD4. The recombinant vector of SlGSTD3 and SlGSTD4 both showed antioxidant activity after exposure to cumene hydroperoxide. Further modeling and docking analysis indicated that the 3D structure of SlGSTD3 and SlGSTD4 were well shaped for phoxim and chlorpyrifos, and the binding affinity for phoxim was stronger than that of chlorpyrifos. Our work provides evidence that SlGSTd3 and SlGSTd4 both play roles in phoxim and chlorpyrifos resistance in S. litura.

BK Knockout by TALEN-Mediated Gene Targeting in Osteoblasts: KCNMA1 Determines the Proliferation and Differentiation of Osteoblasts

Hei, Hongya,Gao, Jianjun,Dong, Jibin,Tao, Jie,Tian, Lulu,Pan, Wanma,Wang, Hongyu,Zhang, Xuemei Korean Society for Molecular and Cellular Biology 2016 Molecules and cells Vol.39 No.7

Large conductance calcium-activated potassium (BK) channels participate in many important physiological functions in excitable tissues such as neurons, cardiac and smooth muscles, whereas the knowledge of BK channels in bone tissues and osteoblasts remains elusive. To investigate the role of BK channels in osteoblasts, we used transcription activator-like effector nuclease (TALEN) to establish a BK knockout cell line on rat ROS17/2.8 osteoblast, and detected the proliferation and mineralization of the BK-knockout cells. Our study found that the BKknockout cells significantly decreased the ability of proliferation and mineralization as osteoblasts, compared to the wild type cells. The overall expression of osteoblast differentiation marker genes in the BK-knockout cells was significantly lower than that in wild type osteoblast cells. The BK-knockout osteoblast cell line in our study displays a phenotype decrease in osteoblast function which can mimic the pathological state of osteoblast and thus provide a working cell line as a tool for study of osteoblast function and bone related diseases.

Molecular and Cellular Microbiology / Biomedical Sciences : Alteration of the Metabolome Profile in Endothelial Cells by Overexpression of miR-143/145

( Wenshuo Wang ),( Ye Yang ),( Yiqing Wang ),( Liewen Pang ),( Jiechun Huang ),( Hongyue Tao ),( Xiaotian Sun ),( Chen Liu ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.3

Communication between endothelial cells (ECs) and smooth muscle cells (SMCs) via miR-143/145 clusters is vital to vascular stability. Previous research demonstrates that miR-143/145 released from ECs can regulate SMC proliferation and migration. In addition, a recent study has found that SMCs also have the capability of manipulating EC function via miR-143/145. In the present study, we artificially increased the expression of miR-143/145 in ECs, to mimic a similar change caused by miR-143/145 released by SMCs, and applied untargeted metabolomics analysis, aimed at investigating the consequential effect of miR-143/145 overexpression. Our results showed that miR-143/145 overexpression alters the levels of metabolites involved in energy production, DNA methylation, and oxidative stress. These changed metabolites indicate that metabolic pathways, such as the SAM cycle and TCA cycle, exhibit significant differences from the norm with miR-143/145 overexpression.

A fluorescent probe for colorimetric detection of bisulfite and application in sugar and red wine

Haitao Chen,Xiaoming Wu,Jialin Wang,Hao Wang,Feiyan Tao,Shaoxiang Yang,Hongyu Tian,Yongguo Liu,Baoguo Sun 한국식품과학회 2019 Food Science and Biotechnology Vol.28 No.4

A new fluorescent probe made from (E)-2-(benzo[d]thiazol-2-yl)-3-(6-hydroxynaphthalen-2-yl) acrylonitrile(Probe 1) was synthesized for the determination ofbisulfite concentrations in real food samples (red wine andsugar). Adding bisulfite to a Probe 1 solution caused amarked decrease in fluorescence intensity and a visualcolor change from yellow to light yellow. This distinctcolor response indicates that Probe 1 could be used as avisual sensor for bisulfite. Probe 1 can detect bisulfitequantitatively in the range 0–400 lM with a detection limitof 0.10 lM. This makes Probe 1 a convenient signalinginstrument for determining bisulfite levels in sugar and redwine samples.

BK Knockout by TALEN-Mediated Gene Targeting in Osteoblasts: KCNMA1 Determines the Proliferation and Differentiation of Osteoblasts

Xuemei Zhang,Hongya Hei,Jianjun Gao,Jibin Dong,Jie Tao,Lulu Tian,Wanma Pan,Hongyu Wang 한국분자세포생물학회 2016 Molecules and cells Vol.39 No.7

Large conductance calcium-activated potassium (BK) channels participate in many important physiological functions in excitable tissues such as neurons, cardiac and smooth muscles, whereas the knowledge of BK channels in bone tissues and osteoblasts remains elusive. To investigate the role of BK channels in osteoblasts, we used transcription activator-like effector nuclease (TALEN) to establish a BK knockout cell line on rat ROS17/2.8 osteoblast, and detected the proliferation and mineralization of the BK-knockout cells. Our study found that the BK-knockout cells significantly decreased the ability of proliferation and mineralization as osteoblasts, compared to the wild type cells. The overall expression of osteoblast differentiation marker genes in the BK-knockout cells was significantly lower than that in wild type osteoblast cells. The BK-knockout osteoblast cell line in our study displays a phenotype decrease in osteoblast function which can mimic the pathological state of osteoblast and thus provide a working cell line as a tool for study of osteoblast function and bone related diseases.