간 세포암에서 VEGF, TGF-β1, b-FGF 발현의 의의

김성용,남충현,주종우,채만규,백무준,이문수,김형철,안현철,김홍수,김창진,김창호 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.1

Purpose: Angiogenesis is important for the proliferation and the metastasis of solid tumors. The growth of a solid tumor is widely recognized to depend on the process of neovascularrozation. Without angiogenesis, tumors cease to grow beyond even a few milimeters in diameter. It has been shown that tumor vascular density is an independent prognostic marker in several types of human tumors and is known to correlate with poor prognosis. To date, many angiogenic factors have been identified, such as transforming growth factor-α(TGF-α), transforming growth factor-β(TGF-β), fibroblast growth factor family(FGF), vascular endothelial growth factor(VEGF), platelet derived endothelial cell growth factor(PD-ECGF), tumor necrosis factor-α(TNF-α), and angiogenin. Hepatocellular carcinoma(HCC) is the second most common tumor in Korean males and is known as a typical hypervascular tumor with frequent portal vein invastion. The authors identified the expreesion of VEGF, TGF-β1, and b-FGF in HCC specimens and evaluated the relationship between these growth factors and the clinicopathologic characteristics of HCC. Method: We reviewed the medical records of 30 patients who were diagnosed as hepatocellular carinoma treated with hepatic resection between January 1994 and December 1998 in Soonchunhyang University Chunan Hospital. The selection of the cases was decided according to the condition of paraffin block fixation. The prognostic factors such as age, sex, tumor size, concentration of serum α-fetoprotein, presence of liver cirrhosis, presence of tumor emboli in portal vein, TMN stage, amount of transfusion during the operation, hepatitis B virus(HBV) infection, and Edmonson-Steiner(E-S) grade were investigated. Relationship between the prognostic factors and the immunopathologic expression of the TGF-β1, b-FGF, and VEGF was examined. Result: Thirty patients (24 males, 6 females) were included in the current study. The patient's mean age was 50.6 years and the age ranged from 36 to 65 years. The mean size of the tumor was found to be 5.2cm. All the patients were follewed up for 7 to 63 months. Child's classification A patients were 23(76.7%)cases, B patients were 7(23.3%)cases, and C was none. Immunohistochemical staining of HCC tumor mass in VEGF expression patients were 17(56.7%), b-FGF expression patients were 10(33.3%), and TGF-β1 expression patients were 10(33.3%). VEGF expression or more than one positive expression among the three factors correlated with tumor size and the stage of HCC but did not correlated with other clinicopathological characteristics. TGF-β1 and b-FGF did not correlate with any clinicopathological characteristics. Conclusion: The results suggest that the expression of VEGF or more than one positive expression among the three factors in HCC cells may be a significant prognostic factor of HCC.

Electropulsing Treatment on Enhancement of Electrical Conductivity of Screen-Printed Ag Wire

Ju‑Won Park,Howook Choi,Hwangsun Kim,Simoon Sung,Hye‑Jin Jeong,Il Kim,Jaeseok Gong,Sung‑Tae Hong,Heung Nam Han 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.5

The effect of high electric current density on the sintering of Ag wires manufactured by screen printing is evaluated throughelectrical resistivity analysis and microstructure observation. Different forms (continuous and pulsed) of electric currentwith different current densities are applied to the specimens. Conventional heat treatment is also performed as a controlgroup to examine the athermal effect of electropulsing treatment. Compared to the conventional heat treatment, the resistivityis reduced more under the electropulsing treatment with continuous current for the same temperature and treatment time.Also, the process time of electropulsing treatment can be reduced by applying a pulse form of high density current insteadof continuous current without losing the benefit of enhanced reduction of resistivity. The microstructural observationsobtained from high angle annular dark field scanning transmission electron microscope and a digital precession instrumentclearly show that necking connecting the crystals is formed more firmly under electric current. In addition, the temperaturechange of Ag wire and substrate is calculated according to the change of the resistivity when the electric current is appliedto confirm the reliability.

Breeding of Tetraploid in Codonopsis lanceolata (Sieb. et Zucc.) Trautvetter by Colchicine Treatment

Kim,Ik-Hwan,Kim,Hag-Hyun,Hong,Eui-Yon,Yun,Jong-Sun,Yun,Tae,Hwang,Ju-Kwang,Lee,Cheol-Hee 한국자원식물학회 2003 Plant Resources Vol.6 No.3

Present studies were carried out to produce tetraploid plants by colchicine treatment using seeds, seedlings and shoot tips of Codonopsis lanceolata. Three tetraploid plants of C. lanceolata were produced from seeds which absorbed 0.1 % colchicine solution for 12 hours, and 0.5% colchicine solution for 1 and 6 hours from seedlings, respectively. But tetraploid was not produced from shoot tips treated by colchicine solution. Compared to diploid, tetraploid plants had larger stomata, but less number of stomata. Fresh weight of tetraploid plants was 1.4∼3.6 times heavier than diploid plants.

황색포도구균의 균혈증에서 초기 항생제 치료가 예후에 미치는 영향

김성한,박완범,이기덕,강철인,최영주,김홍빈,박상원,김의석,오명돈,김의종,최강원 대한감염학회 2002 감염 Vol.34 No.5

혈우병 환자에 동반된 자발성 후복막강 출혈

김광일,김동호,우상민,이석주,김홍성,조인성,윤환중,조덕연,김삼용 충남대학교 의과대학 지역사회의학연구소 1997 충남의대잡지 Vol.24 No.1

Spontaneous retroperitoneal hemorrhage due to hemophilia A with impaired coagulopathy is very rare. Spontaneous retroperitoneal hemorrhage has been recorded as having originated from many retroperitoneal organs and blood vessels, and it may be due to local and/or systemic factors. In the majority of the patients, kidney and adrenal gland were the major site of hemorrhage. The systemic causes of spontaneous retroperitoneal hemorrhage are anticoagulation therapy and chronic hemodialysis. During the course of these treatments, hemorrhagic complications may occur at many site, including the retroperitoneal space. Blood dyscrasias including leukemia, polycythemia, sickle cell trait and hemophilia have been reported associated with spontaneous retroperitoneal hemorrhage. We report a case of spontaneous retroperitoneal hemorrhage occurred in a gemophilia A patient with brief review of literature

Breeding of Tetraploid in Platycodon grandiflorum (Jacq.)A. DC. by Colchicine treatment

Kim,Ik-Hwan,Kim,Hag-Hyun,Hong,Eui-Yon,Yun,Jong-Sun,Yun,Tae,Hwang,Ju-Kwang,Lee,Cheol-Hee 한국자원식물학회 2003 Plant Resources Vol.6 No.3

Present studies were carried out to produce tetraploid plants by colchicine treatment using seeds, seedlings and shoot tips of Platycodon grandiflorum in Campanulaceae. The most successful colchicine treatment for tetraploid production in P. grandiflorum was soaking treatment using 0.01 and 0.5% colchicine solution for 1 hour and 12 hours, respectively. Morphological characteristics of both diploid and tetraploid were similar, but tetraploid plants had more leaves. Compared to diploid, tetraploid had the larger stomata, but less number of stomata. Fresh weight of tetraploids was 20∼40% heavier than that of diploid.

IgG GM₁ 항체와 Campylobacter jejuni 항체와 관련된 급성 운동축삭형 신경병증

홍경훈,김승현,이영배,이영주,김희태,김주한,김명호 대한신경과학회 1997 대한신경과학회지 Vol.15 No.5

상징어의 의미 영역 분류 연구(3)- 감각, 감정, 생각을 나타내는 의태어를 중심으로-

김홍범 ( Hong Beom Kim ),이영주 ( Young Ju Lee ) 한말연구학회 2012 한말연구 Vol.- No.31

Based on the semantic description model of Hong-Beom Kim [2008], we utilized the Microsoft Excel and analyzed 339 symbolic words in the「Classification Dictionary of Korean Onomatopoeic and Mimetic Words」. They found the limit of the consistency and systematic method of the semantic description contents by analyzing「Classification Dictionary of Korean Onomatopoeic and Mimetic Words」. It is difficult to systematize human`s psychology, emotion and the like. We classified the sectors as sense, emotion and perception to solve this problem. The results of the analysis for symbolic words based on the methology of this paper remain to be studied.

전주시 도시경관의 변화과정에 관한 연구

서주환,김홍균,승지현,김옥경,최선주,박선영 경희대학교 부설 디자인연구원 2007 예술· 디자인학연구 Vol.10 No.1

The object of this research is about how jeon-ju, which is one of the historical cities is changed according to the flow of generation. In this analysis, product and enemy factors will be studied. The inner and urban landscape of the generations of the 1860s, 1930s and modern times will be surveyed and compared in the context to grasp those factors. Analysis will be applied to this modern historical city by theorizing how these factors will effect future development. Today's modern landscape will be compared and analyzed based on the effects and abstractions of the recent landscape immediately preceeding current times. Modern conditions will again be placed in context, by suggesting future potential developments. In the result, first, considering present conditions of the landscape which set the stage for the Japanese invasion, an evaluation will be made on the best course of action for future development of street landscape. Second, the question of preserving the history and culture of this rich and ancient city through network of modern street landscape naturally will be posed. Finally, an analysis of the difference between the old and modern landscape will be evaluated, in preparation for suggestions of future development.

출류(朮類)한약재의 외,내부형태와 이화학패턴 연구

김정훈 ( Jung Hoon Kim ),이금산 ( Geum San Lee ),최고야 ( Go Ya Choi ),황성연 ( Sung Yeoun Hwang ),김홍준 ( Hong Jun Kim ),정승일 ( Seung Il Jeong ),주영승 ( Young Sung Ju ) 대한본초학회 2009 大韓本草學會誌 Vol.24 No.2

Objectives: To determine the discriminative criteria for Atractylodes rhizomes, the experiment of external-internal characteristics and physicochemical pattern analysis were performed. Methods: External characteristics was observed using stereoscope. The sectioned materials which were covered with parffin were stained by Ju`s method. Physicochemical patterns were analyzed using HPLC/DAD. Results: 1. External shape of original plant: Atractylodes macrocephala and A. japonica had relatively long petioles and 3-5 parted leaves. A. macrocephala had big purple flowers whereas A. japonica had relatively small white flowers and pinnate bracts. A. lancea had sessile leaves and white flowers, and the end parts of degenerated stamens were bent. 2. External shape of herbal medicine: A. macrocephala which was fist-shaped rhizome had pa-pillate processes and the cross section was light gray and sulcate. A. japonica and A. lancea were connected-beady or tubercular rhizomes, and the cross sections were both yellow-colored white. However, the cross section of A. japonica was fibrous, the width of cortex was narro-wer than that of stele, and radial shape in cortex was rare, whereas the width of cortex in A. lancea was similar to that of stele in size, and radial shape in cortex was obvious. 3. Internal shape of herbal medicine: A. macrocephala and A. lancea did not have lignified fascicles in cortex. However, the vascular bundles and vessels of A. macrocephala were wedge shaped and radial arrangement, and vascular bundles were densely populated in stele whereas those of A. lancea were repeatedly arranged and thinly extended to cortex. A. japonica had lignified fascicles in cortex and the width of vascular bundles was conspicuously thick with narrow intervals. 4. Physicochemical pattern analysis: A. macrocephala and A. lancea contained atractylenolide Ⅰ and atractylenolide Ⅲ whereas A. japonica contained atractylenolide Ⅰ, atractylenolide Ⅲ, diacetyl-atractylodiol, compound-4, compound-5. The three species of Atractylodes rhizomes showed different chromatogram patterns. Conclusions: The results could be used as discriminative criteria for Atractylodes rhizomes and as fundamental materials to researches of further pattern analysis and biological reaction.