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RT-LAMP를 이용한 콩황화일반모자이크바이러스의 진단
배대현, 이영훈, 김봉섭, 윤영남, 강범규, 최만수, 구성철, 김현태, 윤홍태, 이수헌, 백인열 忠北大學校 農業科學硏究所 2014 農業科學硏究 Vol.30 No.2
Soybean yellow common mosaic virus (SYCMV) has been recently reported, it has been occurred a lot with Soybean mosaic virus (SMV) and Soybean yellow mottle mosaic virus (SYMMV) in soybean field. SYCMV belongs to genus of Sobemovirus and induced viral symptoms with yellowing, mottle and mosaic. A reverse transcription loop-mediated isothermal amplification (RT-LAMP) method allowed one-step detection of gene amplification by simple procedure and needed only a simple incubator for isothermal template. This RT-LAMP method allowed direct detection of RNA from virus-infected plants without thermal cycling and gel electrophoresis. In this study, we designed RT-LAMP primers named SYCML-F3/B3/FIP/BIP from coat protein gene sequence of SYCMV. After the reaction of RTLAMP, products were identified by electrophoresis and with the detective fluorescent dye, SYBR Green I. under daylight and UV light. Optimal reaction condition was at 63 for 60min and the primers of RTLAMP showed the specificity for only SYCMV tested in this study.
Bae, Jiwoong,Lim, Yonghyun,Park, Jun-Sik,Lee, Dohaeng,Hong, Soonwook,An, Jihwan,Kim, Young-Beom The Electrochemical Society 2016 Journal of the Electrochemical Society Vol.163 No.8
<P>Grain boundaries are known to block ionic conduction across grain boundaries in oxide ion conductors due to adjacent space charge layers. Since the positively charged grain boundary core is intensified with a high local concentration of defects such as oxygen vacancies, uniform distribution of a dopant may mitigate the formation of space charge layers and enhance the ionic conductivity. To investigate the dopant segregation effect on the space charge layer and ionic conductivity, we provided thermal energy to nanocrystalline gadolinia-doped ceria (GDC) thin film by post-annealing at different temperatures of 700 degrees C, 900 degrees C, and 1100 degrees C. STEM-EELS analysis demonstrates strong dopant segregation and a higher Ce3+ content near the grain boundary than in the bulk after post-annealing. The concurrent segregation of dopants and Ce3+ ions implies that once thermal treatment is applied to nanocrystalline GDC thin films, complete space charge layers are formed while the non-thermally treated GDC film with a uniform distribution of dopants has less of a space charge effect and exhibits superior ionic conductivity. (C) 2016 The Electrochemical Society. All rights reserved.</P>
Bae, Sung Jin,Kim, Beom-Jun,Lim, Kyeong Hye,Lee, Seung Hun,Kim, Hong Kyu,Kim, Ghi Su,Koh, Jung-Min Japanese Society of Bone and Mineral Metabolism ; 2012 Journal of bone and mineral metabolism Vol.30 No.5
<P>We investigated rates of insufficient and over-responsiveness to orally administered bisphosphonates in postmenopausal women, and tested the efficacy of intravenous ibandronate in patients with insufficient response to orally administered bisphosphonates. Postmenopausal women were treated with either alendronate (70?mg/week; n?=?88) or risedronate (35?mg/week; n?=?84) for 1?year, and their response to orally administered bisphosphonates was assessed using serum C-telopeptide (CTX) levels. Insufficient responders were changed to once-quarterly intravenous ibandronate 3?mg injection (n?=?13) or maintained on orally administered bisphosphonates (n?=?19), according to patients' preference, for an additional 1?year. There was no significant difference in baseline characteristics between two orally administered bisphosphonate groups except the bone mineral density values at the lumbar spine. Insufficient rate was higher in the risedronate group (19.0?%) than in the alendronate group (8.0?%), using the premenopausal serum CTX median as a cut-off (P?=?0.043). The over-response rate among the alendronate group (59.1?%) was significantly higher than that in the risedronate group (38.1?%), based on a serum CTX cut-off value of 0.100?ng/ml (P?=?0.006). Intravenous ibandronate suppressed serum CTX levels to a significantly greater degree at 7?days after the second dosing (0.191??0.110?ng/mL; P?<?0.001) and 3?months after the fourth dosing (0.274??0.159?ng/mL; P?=?0.004) among insufficient responders, compared with post-oral/pre-intravenous levels (0.450??0.134?ng/mL). Rates of insufficient and over-responsiveness to orally administered bisphosphonates were considerable, and a change to intravenous bisphosphonates may be considered in patients showing an insufficient response to orally administered bisphosphonates.</P>
Bae, Jiwoong,Chang, Ikwhang,Kang, Sungmin,Hong, Soonwook,Cha, Suk Won,Kim, Young Beom American Scientific Publishers 2014 Journal of nanoscience and nanotechnology Vol.14 No.12
<P>The effects of a post-annealing treatment on the performance of low-temperature solid oxide fuel cells (LT-SOFCs) were investigated. Nickel oxide-samarium doped ceria (NiO-SDC) anodes and yttria stabilized zirconia (YSZ) electrolytes were deposited on anodized aluminum oxide (AAO) membranes by RF sputtering and DC reactive sputtering, respectively. The half-cell of YSZ/NiO-SDC was then heat-treated at 600 degrees C for 10 h, and a porous platinum (Pt) cathode was deposited on the annealed YSZ/NiO-SDC structure by DC magnetron sputtering. Electrochemical impedance spectroscopy (EIS) analysis revealed a significant decrease in the ohmic resistance and a slight increase in the cathodic impedance. Such a result may be attributed to the increased grain size and enhanced crystallinity of the YSZ electrolyte after the heat treatment. The maximum power density observed for the heat-treated cell was 35 mW/cm2 at 450 degrees C, more than three times higher than the 10 mW/cm2 value obtained for the as-deposited cell.</P>
High phytosterol levels in corn cobs point to their sustainable use as a nutritional source
Bae Hwan-Hee,Ha Jun Young,Go Young Sam,Son Jae-Han,Son Beom-Young,Kim Jae-Hong,Shin Seonghyu,Jung Tae-Wook,Yi Gibum 한국응용생명화학회 2022 Applied Biological Chemistry (Appl Biol Chem) Vol.65 No.6
Phytosterols are important structural components of plant cells that affect membrane fluidity, permeability, and membrane-related metabolic regulation. These compounds, which are abundant in vegetable oils and corn kernel oil, are also beneficial for human health. Cultivation of corn (Zea mays L.) produces huge amounts of cobs as a byproduct, but efforts to utilize cobs are still limited. Here, we investigated phytosterol, crude oil, and fatty acid contents in the kernels and cobs of four major corn cultivars in South Korea and explored the potential use of cobs as a source of phytosterols. Total phytosterol levels were two times higher in cobs (68.0–217.1 mg 100 g− 1 DW) than in kernels (43.8–89.5 mg 100 g− 1 DW) and were highest in the kernels and cobs of Sinhwangok at 60 days after pollination. We showed that not only kernels but also cobs can be a rich source of phytosterols. The results also revealed that the amount of phytosterol is depending on a genetic background as well as developmental stages suggesting further investigation would enhance the utilization of corn cobs as a phytosterol source. Phytosterols are important structural components of plant cells that affect membrane fluidity, permeability, and membrane-related metabolic regulation. These compounds, which are abundant in vegetable oils and corn kernel oil, are also beneficial for human health. Cultivation of corn ( Zea mays L.) produces huge amounts of cobs as a by-product, but efforts to utilize cobs are still limited. Here, we investigated phytosterol, crude oil, and fatty acid contents in the kernels and cobs of four major corn cultivars in South Korea and explored the potential use of cobs as a source of phytosterols. Total phytosterol levels were two times higher in cobs (68.0–217.1 mg 100 g −1 DW) than in kernels (43.8–89.5 mg 100 g −1 DW) and were highest in the kernels and cobs of Sinhwangok at 60 days after pollination. We showed that not only kernels but also cobs can be a rich source of phytosterols. The results also revealed that the amount of phytosterol is depending on a genetic background as well as developmental stages suggesting further investigation would enhance the utilization of corn cobs as a phytosterol source.