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Multidrug and Toxic Compound Extrusion Protein-1 (MATE1/SLC47A1) Is a Novel Flavonoid Transporter
Lee, Ji Hae,Lee, Jung Eun,Kim, Yeojin,Lee, Hojoung,Jun, Hee-jin,Lee, Sung-Joon American Chemical Society 2014 Journal of agricultural and food chemistry Vol.62 No.40
<P>Dietary flavonoids have various biological functions. However, their cellular transport mechanisms are largely unknown. We have determined that the multidrug and toxic compound extrusion transporter-1 (MATE1) is a membrane transporter for flavonoids and has a high affinity for quercetin. HEK293T cells overexpressing MATE1 exhibited increased intracellular quercetin accumulation. This effect disappeared in the presence of a MATE1 inhibitor and after MATE1 gene knockdown. HepG2 cells expressed MATE1 significantly, with the uptake quercetin of which was dramatically reduced with MATE1 inhibition. On the basis of immunofluorescence analysis, MATE1 was highly expressed in peroxisomes and the endoplasmic reticulum (ER) as well as in plasma membranes in the liver and intestine, which suggests potential accumulation of quercetin in peroxisomes and the ER in these tissues. Fluorescent microscopic analysis confirmed selective accumulation of qurcetin in peroxisome. The effects of quercetin on cellular lipid reduction and glucose uptake were exaggerated with MATE1 overexpression. In conclusion, MATE1 is a membrane transporter for quercetin; its overexpression enhances the hypolipidemic activity of quercetin and cellular glucose transport. Considering the low bioavailability of quercetin, appropriate regulation of MATE1 expression may optimize cellular quercetin concentrations and promote health benefits.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jafcau/2014/jafcau.2014.62.issue-40/jf500916d/production/images/medium/jf-2014-00916d_0004.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/jf500916d'>ACS Electronic Supporting Info</A></P>
Effect of sodium silicate on early growth stages of wheat under drought stress
Lee Sang Gyu,Lee Hyeri,Lee Byung Cheon,Lee Hojoung,Moon Jun Cheol,Choi Changhyun,Chung Namhyun 한국응용생명화학회 2020 Applied Biological Chemistry (Appl Biol Chem) Vol.63 No.4
Wheat yield is decreasing due to climate change, and a method to prevent decreasing yield during drought stress is desirable. In this study, wheat cultivars (Koso and Jokyung) were treated with 15% polyethylene glycol-6000 (PEG) and PEG + Si solution (6.5, 8.7, 13.1 and 26.1 mM). The effect of Si treatment on the alleviation of drought stress was measured using the germination test, shoot relative water content (RWC), seedling stage observation, and quantitative real time polymerase chain reaction (qRT-PCR). The results of root/shoot length ratio and shoot length ratio showed that Si treatment induced the alleviation of drought stress in Jokyung cultivar. The result of qRT-PCR showed the alleviation of drought stress in Koso cultivar. In addition, the results with shoot RWC and seedling stage observation showed that the alleviation effects of Si treatment was observed with both Koso and Jokyung cultivar at the high concentration of Si (26.1 mM). All these results suggest that Si treatment at a high concentration could be employed to alleviate drought stress in wheat.
Lee Dong Gun,Lee Ji Min,Choi Chang Geun,Lee Hojoung,Moon Jun Cheol,Chung Namhyun 한국응용생명화학회 2021 Applied Biological Chemistry (Appl Biol Chem) Vol.64 No.6
Salinity stress is a serious abiotic stress that affects crop quality and production. Rhizospheric microbes have immense potential in synthesizing and releasing various compounds that regulate plant growth and soil physicochemical properties. The aim of the present study was to evaluate the efficacy of indole-3-acetic acid (IAA)-producing rhizobacteria as biofertilizers under salt stress. Among the isolated strains from various soil samples, Bacillus megaterium strain PN89 with multifarious plant growth-promoting traits was selected and used as a monoculture and co-culture with two other standard strains. The plant promoting activity was evaluated using the paper towel method and pot test to observe the effects on the early stage and vegetative growth of wheat (Triticum aestivum L.). The treatment using PGPR strain presented noticeable but varying effects on plant growth under salt stress, that is, PGPR treatment often displayed a significant increase in germination percentage, root and shoot length, and other growth parameters of wheat compared to those in the non-inoculated control. Thus, these results suggest that B. megaterium PN89 can be applied as a bio-fertilizer to alleviate salt stress in T. aestivum.
( Won Je Lee ),( Chan Young Jeong ),( Seokjin Lee ),( Chon-sik Kang ),( Hojoung Lee ) 한국응용생명화학회(구 한국농화학회) 2019 Journal of Applied Biological Chemistry (J. Appl. Vol.62 No.1
All countries, including Korea, are currently experiencing the effect of rapid climate change. As a result, the cultivation area of many crops including wheat is changing, or productivity is falling sharply. If enough nitrogen is present in the soil, the increase in atmospheric carbon dioxide due to the greenhouse effect can lead to increased photosynthesis of plants, resulting in increased productivity. By contrast, a low proportion of nitrogen in soil does not increase production, often leading to the use of nitrogen fertilizers to increase crop productivity: this causes serious environmental pollution due to the leakage of nitrogen fertilizers used by crops. Increasing the understanding of the molecular level of the plant nitrogen use efficiency mechanism may contribute to increased productivity of crops and reduced of environmental pollution by nitrogen. In Korea, cultivars have developed 35 kinds of wheat, such as ‘keumgang’ and ‘Chokyeong’, which can be used for specific purposes such as baking or noodles. In this study we investigate ‘keumgang’ and ‘Chokyeong’ in order to elucidate the mechanism of nitrogen use ability of wheat and contribute to the reduction of environmental pollution by providing guidelines for the proper use of nitrogenous fertilizer.
Trinh, Cao Son,Lee, Hyeri,Lee, Won Je,Lee, Seok Jin,Chung, Namhyun,Han, Juhyeong,Kim, Jongyun,Hong, Suk-Whan,Lee, Hojoung Springer 2018 Plant cell reports Vol.37 No.6
<P>Plant growth-promoting rhizobacteria (PGPR) enhance plant development through various mechanisms; they improve the uptake of soil resources by plants to greatly promote plant growth. Here, we used Arabidopsis thaliana seedlings and Lactuca sativa to screen the growth enhancement activities of a purified PGPR, Pseudomonas nitroreducens strain IHB B 13561 (PnIHB). When cocultivated with PnIHB, both species of plants exhibited notably improved growth, particularly in regard to biomass. Quantitative reverse transcription polymerase chain reaction analysis indicated high expression levels of the nitrate transporter genes, especially NRT2.1, which plays a major role in the high-affinity nitrate transport system in roots. Moreover, enhanced activity of the cyclin-B1 promoter was observed when wild-type 'Columbia-0' Arabidopsis seedlings were exposed to PnIHB, whereas upregulation of cyclin-B also occurred in the inoculated lettuce seedlings. Overall, these results suggest that PnIHB improves A. thaliana and L. sativa growth via specific pathways involved in the promotion of cell development and enhancement of nitrate uptake.</P>
Koh, Eun-Ji,Lee, Sung June,Hong, Suk-Whan,Lee, Hoi Seon,Lee, Hojoung Korean Society for Molecular Biology 2008 Molecules and cells Vol.26 No.3
Invertase (β-D-fructofuranosidase; EC 3.2.1.26) catalyzes the conversion of sucrose into glucose and fructose and is involved in an array of important processes, including phloem unloading, carbon partitioning, the response to pathogens, and the control of cell differentiation and development. Its importance may have caused the invertases to evolve into a multigene family whose members are regulated by a variety of different mechanisms, such as pH, sucrose levels, and inhibitor proteins. Although putative invertase inhibitors in the Arabidopsis genome are easy to locate, few studies have been conducted to elucidate their individual functions in vivo in plant growth and development because of their high redundancy. In this study we assessed the functional role of the putative invertase inhibitors in Arabidopsis by generating transgenic plants harboring a putative invertase inhibitor gene under the control of the CaMV35S promoter. A transgenic plant that expressed high levels of the putative invertase inhibitor transcript when grown under normal conditions was chosen for the current study. To our surprise, the stability of the invertase inhibitor transcripts was shown to be down-regulated by the phytohormone ABA (abscisic acid). It is well established that ABA enhances invertase activity in vivo but the underlying mechanisms are still poorly understood. Our results thus suggest that one way ABA regulates invertase activity is by down-regulating its inhibitor.
Truong, Hai An,Lee, Won Je,Jeong, Chan Young,Trị,nh, Cao Sơ,n,Lee, Seokjin,Kang, Chon-Sik,Cheong, Young-Keun,Hong, Suk-Whan,Lee, Hojoung Elsevier 2018 Journal of plant physiology Vol.231 No.-
<P><B>Abstract</B></P> <P>Plants require nitrogen (N) for growth and development. However, they are frequently exposed to conditions of nitrogen deficiency. In addition, anthocyanin accumulation is induced under salt stress and nitrate deficiency. To date, most studies have revealed that nitrate deficiency under high sucrose levels induce high levels of anthocyanin accumulation in plants. However, the underlying mechanisms remain unclear. Under nitrate-starved conditions, plant growth rapidly worsens and cells eventually die. In addition, plants are severely affected by salt exposure. Therefore, in this study, we determined whether increased levels of anthocyanin could improve plant growth under salt stress and nitrate-starved conditions. We used <I>PAP1-D/fls1ko</I> and <I>ttg1</I> plants which have a perturbed anthocyanin biosynthesis pathway to explore the role of anthocyanin in plant adaptation to nitrate-deficient conditions and salt stress. Our results demonstrate that high anthocyanin accumulation in <I>PAP1-D/fls1ko</I> plants confers enhanced tolerance to nitrate-deficient conditions combined with high salinity. <I>PAP1-D/fls1ko</I> plants appeared to use absorbed nitrate efficiently during the nitrate reduction process. In addition, nitrate-related genes such as <I>NRT1.1, NiA1</I> and <I>NiA</I>2 were upregulated in the <I>PAP1-D/fls1ko</I> plants. On the basis of these findings, it can be concluded that high anthocyanin accumulation helps plants to cope with salt stress under nitrate-deficient conditions via the effective utilization of nitrate metabolism.</P>
Truong, Hai An,Jeong, Chan Young,Lee, Won Je,Lee, Byung Cheon,Chung, Namhyun,Kang, Chon-Sik,Cheong, Young-Keun,Hong, Suk-Whan,Lee, Hojoung American Chemical Society, Books and Journals Divi 2017 Journal of agricultural and food chemistry Vol.65 No.28
<P>Thermotolerance in plants is a topic of concern given the current trends in global warming. Here, we aimed to develop a rapid and reproducible screening method for selection of heat stress-tolerant wheat varieties to expedite the breeding process. We tested the robustness of the screen in three Korean wheat cultivars, 'BackJung', 'KeumKang', and 'ChoKyeong'. We showed that 4-day-old seedlings of 'KeumKang' had the highest survival rates after a 45 degrees C treatment for 20 h. Moreover, the ability to retain chlorophyll and antioxidant activity was also highest in 'KeumKang'. The increase in malondialdehyde content in 'ChoKyeong' indicated that this cultivar showed the greatest damage after heat stress. Collectively, our results showed that 'KeumKang' is the most heat-tolerant cultivar of the three examined. In conclusion, the most reliable and rapid screening method in our investigation was survival rate examined at lethal temperature.</P>