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FK506이 T 림프구 사멸에서 활성산소 생성에 미치는 영향
이호균(Ho Kyun Lee),정상영(Sang Young Chung),최수진나(Soo Jin Na Choi) 대한외과학회 2009 Annals of Surgical Treatment and Research(ASRT) Vol.77 No.5
Purpose: Tacrolimus (FK506) has been widely used as an immunosuppressant in organ transplanted recipients to suppress organ rejection phenomenon. We investigated the role of oxidative stress and heme oxygense-1 by FK506 on human Jurkat T cells. Methods: The cells viability was examined by DAPI stain, enzyme activity of caspase family proteins, and western blotting for Baks, PUMA, iNOS, HO-1. Cells were cultured in the absence or presence of CoPPIX or ZnPPIX and the fluorescence intensity was analyzed using a flow cytometry. Results: Treatment with FK506 increased the generation of reactive oxygen species (ROS), including hydrogen peroxide and superoxide anion, and NO in Jurkat cells in a dose-dependent manner. Immunohistochemistry and Western blot analysis data revealed the hemoxygenase-1 (HO-1) was induced by the addition of FK506 in Jurkat cells. Induction of CoPP, HO-1 inducer, resulted in decreased intracellular H₂O₂ and NO concentrations. Instead ZnPP, an HO-1 competitive inhibitor did it reversely. In addition, ZnPP regulates iNOS protein synthesis by inhibition of HO-1. Conclusion: Increase of HO-1 expression would induce to decrease the intracellular H₂O₂ and NO concentrations. Also, HO-1 would regulate iNOS protein synthesis. Consequently, we can expect the regulation of HO-1 expression with concomitants use of FK506 to suppress organ rejection phenomenon by enhancing apoptosis.
Mycophenolic Acid에 의해 유도된 Jurkat 세포주 세포자멸사에서 Heme Oxygenase-1의 발현이 미치는 영향
이호균(Ho Kyun Lee),최수진나(Soo Jin Na Choi) 대한외과학회 2010 Annals of Surgical Treatment and Research(ASRT) Vol.78 No.6
Purpose: This study demonstrates that pharmacologic induction of heme oygenase-1 (HO-1) along with catalytic activation significantly modulated apoptosis of Jurkat cells induced by mycophenolic acid (MPA). Methods: Cells were cultured with the presence or absence of MPA. Flow cytometric analysis was performed after propidium iodide staining. Western blotting of HO-1, Bcl, and Bax was also performed. Cells were stained 4’-6-Diamidino-2-phenylindole (DAPI) and measured by flow cytometry in the absence or presence of CoPPIX. Results: Treatment of MPA decreased cell viability in a dose- and time-dependent manner. MPA-induced cell death was confirmed as apoptosis characterized by sub G0/G1 phase arrest. Expression of HO-1 assumes a pattern of decline after rising at the initial phase. CoPPIX, HO-1 inducer, significantly inhibited the cisplatin-induced apoptosis. Treatment of MPA resulted in reactive oxygen species (ROS) generation in Jurkat cells. CoPPIX attenuated ROS production in MPA-treated cells. Conclusion: This result suggests that the protective mechanism of HO-1 on MPA-induced cytotoxicity is associated with direct inhibition of ROS generation and mitochondrial permeability transition.
MicroRNA profiling of tacrolimus-stimulated Jurkat human T lympocytes
Ho Kyun Lee,Sang Young Chung,Soo Jin Na Choi 대한외과학회 2013 Annals of Surgical Treatment and Research(ASRT) Vol.85 No.4
Purpose: This study investigated the Jurkat T cell line expresses cytotoxicity when treated with different concentrations of FK506, and analyzed the expression pattern of microRNA when stimulated by FK506 using the microRNAs microarray, as well as the expression pattern of a gene that is related to the differentiation, activation and proliferation of T cells after being affected by the change of microRNAs. Methods: To investigate the effects of FK506 on microRNA expression, we purified total RNA of Jurkat cells treated with 20 μM FK506 for 72 hours and used to analyze microRNA profiling by using Agilent’s chip. Results: These results demonstrated that treatment with FK506 markedly induced the down-regulation of 20 microRNAs as well as the up-regulation of 20 microRNAs in a time-dependent manner. The genes that down-regulated by FK506 include let-7a*, miR-20a*, and miR-487a. Otherwise miR-202, miR-485-5p, and miR-518c* are gradually up-regulated in expression. Sanger Institute and DAVIDs bioinformatics indicated that microRNAs regulated the several transcriptomes including nuclear factor of activated T cell-related, T cell receptor/interleukin-2 signaling, and Ca<SUP>2+</SUP>-calmodulin-dependent phosphatase calcineurin pathways. Conclusion: As a result of treating FK506 to a Jurkat cell line and running the microRNA microarray, it was found that FK506 not only took part in the suppression of T cell proliferation/activation by inhibiting calcineurin in Jurkat apoptosis, but also affected the microRNAs that are involved in the regulation of various signal transduction pathways.
LuxS and smcR Quorum-Sensing System of Vibrio vulnificus as an Important Factor for In Vivo Survival
( Na Ri Shin ),( Chang Ho Baek ),( Deog Yong Lee ),( Young Wook Cho ),( Dae Kyun Park ),( Ko Eun Lee ),( Kun Soo Kim ),( Han Sang Yoo ) 한국미생물생명공학회 2005 Journal of microbiology and biotechnology Vol.15 No.6
Changes in suprarenal and infrarenal aortic angles after endovascular aneurysm repair
Ho Kyun Lee,Sang Young Chung,Jea Kyu Kim,Sung Hee Yoo,Soo Jin Na Choi 대한외과학회 2014 Annals of Surgical Treatment and Research(ASRT) Vol.87 No.4
Purpose: We investigated whether suprarenal and infrarenal aortic angles change after the endovascular aneurysm repair (EVAR) procedure and during follow-up, and investigated the correlation between infrarenal aortic angle after EVAR and type Ia endoleaks. Methods: Data collected on 70 EVAR procedures for a fusiform infrarenal aortic aneurysm performed between May 2006 and December 2012 were supplemented with a retrospective review of charts and radiographs. Results: The greater the preoperative infrarenal aortic angle, the greater the suprarenal aortic angle (r = 0.72, P < 0.001). The infrarenal aortic angle decreased after the EVAR procedure and continued to decrease slowly thereafter (all P < 0.001). Suprarenal aortic angle decreased immediately after the EVAR procedure and continued to decrease during the first month (P < 0.001). No differences in angulation were observed based on stent graft type. Type Ia endoleaks occurred with significantly greater incidence in patients with a larger post EVAR infrarenal angle (P = 0.037). Conclusion: The infrarenal aortic angle decreased significantly immediately after the EVAR procedure and continued to decrease slowly thereafter. Suprarenal aortic angle decreased immediately after the EVAR procedure and continued to decrease during the first month. We found a correlation between infrarenal and suprarenal aortic angle. Type Ia endoleaks occurred with greater incidence in patients with a larger infrarenal angle immediately after EVAR.
Na, Won Kyun,Lim, Hyung Mi,Huh, Soo Hyun,Park, Sang Eon,Lee, Youn-Seoung,Lee, Seung Ho Elsevier 2009 Materials science and engineering B. Advanced Func Vol.163 No.2
<P><B>Abstract</B></P><P>Colloidal silica was prepared using a direct oxidation process of silicon powders with different purities, average particle sizes, and surface oxidation layer thicknesses in a water solvent with the base catalysts. Purities of 97.00–99.96% and average particle sizes of 200 and 500 mesh of silicon starting materials were evaluated. The materials were thermally oxidized for 3, 16, and 30h to observe the effect of the surface oxidation layer on the formation of colloidal silica. The longer the thermal oxidation time, the higher the oxide layer thickness on the silicon, and the larger the average size of silica particle observed in the product of colloidal silica when silicon particles had the oxide layer up to approximately 50nm. When the oxide film was higher than 50nm, the silica particle size did not increase with an increase in the oxide layer of silicon. The dependence of the average particle silica size on the oxide layer thickness of silicon was observed to be smaller in the colloidal silica from the 200 mesh size of silicon than from the 500 mesh size. The average particle size of silicon was found to affect the average particle size of silica in the direct oxidation, however the correlation between the purity of silicon and the average particle size of silica was not determined as it fell outside the range of this experiment.</P>
Development of defects in ZnO/RGO composites under wet chemical synthesis
Na, Han Gil,Jung, Taek-Kyun,Ryou, Min,Lee, Ji-Woon,Hyun, Soong-Keun,Kang, Sung Yong,Mirzaei, Ali,Bonyani, Maryam,Kim, Kyung-Taek,Choi, Ho-Joon,Kim, Hyoun Woo,Jin, Changhyun Elsevier 2018 Optik Vol.156 No.-
<P><B>Abstract</B></P> <P>ZnO/reduced graphene oxide (RGO) heterostructures were fabricated using a simple two-step wet chemical technique and post-annealing treatment. ZnO nanoparticles with different sizes (20–200 nm) and shapes were randomly distributed on mono- and/or multi-layered RGO sheets. The microstructures of the ZnO/RGO composites examined using transmission electron microscopy indicated that the heterostructures are polycrystalline in nature, implying the possibilities of diverse defects present in the samples. The photoluminescence spectra examinations revealed the enhancement of defect-level emission peaks observed at a relatively long wavelength ranges (i.e., 779 nm, 666 nm, and 574 nm) as compared with the band to band transition observed at relatively short wavelengths (i.e., 378 nm).</P>
充塡된 아말감 除去時 發生되는 水銀蒸氣量 測定에 關한 硏究
羅肯均,閔丙淳,崔浩永,朴尙進 大韓齒科保存學會 1984 Restorative Dentistry & Endodontics Vol.10 No.1
The aim of this study was to examine the influence of the speed of grinding and coolants on mercury vaporization during amalgam removal. Forty amalgam filled stone dies were stored at 37℃ and 100% relative humidity for 7 days prior to the beginning of the mercury vapor experiment and were divided into 4 different groups; In Group Ⅰ; Used by high speed without coolant & evacuator during amalgam removal. In Group Ⅱ; Used by high speed with coolant & evacuator during amalgam removal. In Group Ⅲ; Used by low speed without coolant & evacuator during amalgam removal. In Group Ⅳ; Used by low speed with coolant & evacuator during amalgam removal. The amalgam specimens were removed in a 30-second time period and mercury vapor was collected with membrane filter at 27㎜ from the site of removal and 45 degree above there, Samples in Group Ⅱ,Ⅳ were removed with coolant spray at a flow rate of 30 ㎖/min with high-velosity evacuator. Mercury vapor collected membrane filter was analysed by Atomic Absorption Spectrophotometer using cold vapor method. The results were as follows; 1. The mercury vapor levels were obtained all of the Groups. 2. The mercury vapor levels of the Group Ⅱ,Ⅳ (with coolant & evacuator) were less than that of the Group Ⅰ,Ⅲ (without coolant & evacuator). 3. The highest mercury vapor level recorded during amalgam removal procedure was Group Ⅰ (used by high speed without coolant & evacuator) and its record was 0.78±0.09 ㎎/㎥, which exceed the T.L.V. by 15 times. 4. The mercury vapor level of the Group Ⅳ (used by low speed with coolant & evacuator) was more than that of the Group Ⅱ (used by high speed with coolant & evacuator), but its difference was not significant, statistically. (p>0.05)