도시의 모험놀이터‘플레이파크’에 관한 고찰

신순호(Shin Soon Ho),박성현(Park Sung Hyun) 한국도시행정학회 2011 도시 행정 학보 Vol.24 No.1

The purpose of this study is to suggest a preliminary data based upon implications from the analysis of Japan’ s Play Park that how we could introduce Play Park in the urban environment. With this in mind, We have looked at the concept of Play Park, the history of Play Park, and the role of Play Park. After that, We have examined “Hanegi Play Park”, which is known for the most advanced model in Japan, based on the following analytical frameworks. First of all, We identified geographic characteristics (location, area, space, facilities, and tools), and then looked into utilization(number of users, age of users, and contents of play), operating structure(operating system and solidarity), and operating contents and method(finance, safety, program, and insurance). In order to enhance the accuracy of research, We have used the different way of research according to its characteristics such as geographic characteristics with observation and utilization, operating structure, and operating contents and method with interview. After analyses, We have found out that a few thing were shall be considered before it introduced Play Park in urban environment. First, Play Park did not require special components such as condition of site and man-made structures, therefore We suggested that we should consider trying the existing park that hardly uses or becomes obsolete on a trial basis. Second, We suggested that we need to take account of operating solidarity in order to make the best use of Play Park. In other words, it shall be an organic cooperation system that local government limits their role to provide only place and finance, while residents could take charge of Play Park operation and all other matters as a “trust”way. Last but not least, it should be discussed how to raise funds. In case of “Hanegi Play Park”, it consists of two parts with ratio of 3:1; consignment fee from local government and earnings from own business. We, however, need to discuss whether this ratio is reasonable or not. It is more desirable to raise funds through own business as much as possible rather than local government financial support.

Elevational Distribution of Breeding Bird Communities in Seoraksan National Park, Korea

( Hyun-su Hwang ),( Jae-kang Lee ),( Tae-kyung Eom ),( Ho-kyoung Bae ),( Dong-ho Lee ),( Jong-hwan Lim ),( Sung-cheol Jung ),( Chan-ryul Park ),( Shin-jae Rhim ) 한국산림과학회(구 한국임학회) 2020 한국산림과학회지 Vol.109 No.1

In this study, the elevational distribution of breeding birds in Seoraksan National Park, Korea was investigated. Forty-six species of birds were documented from line transect surveys taken from Seorakdong at 230 m above sea level (a.s.l.) to the Daechungbong summit at 1708 m asl. Birdspecies richness and diversity were highest in Seorakdong and lowest at the Daechungbong summit. As elevation increased, bird species richness and diversity decreased, with a humped-shape trend being observed between 700 m and 1200 m a.s.l. Stepwise analyses revealed that breeding bird species diversity indices were significantly negatively correlated with elevation (r² = 0.327, P < 0.001) and positively correlated with vegetational coverage (r² = 0.324, P = 0.046). Higher elevations supported fewer birds than low and intermediate elevations. Projections at the local scale, including data on behavior and habitat use by birds, will be necessary for optimal conservation and management of the bird communities in Seoraksan National Park.

국립공원 내 공중화장실에 대한 탐방객 인식에 관한 연구 : 지리산국립공원을 중심으로

이호(Ho Lee),이승록(Seung-Rok Lee),박기현(Ki-Hyun Park),박은희(Eun-Hee Park),차진열(Jin-Yeol Cha),심규원(Kyu-Won Sim),강동원(Dong-Won Gang) 국립공원연구원 2011 국립공원연구지 Vol.2 No.1

본 연구는 공원 내 공중화장실에 대한 탐방객의 인식이 만족도에 미치는 영향을 규명하고자 수행하였다. 현지 설문조사는 지리산국립공원의 성삼재와 중산리 일원을 방문한 탐방객 292명을 대상으로 이루어졌다. 분석결과, 공중화장실의 관리상태, 외관, 디자인 요인 모두 탐방객의 공중화장실 이용 만족에 영향을 주는 것으로 분석되었으며, 그 중 관리상태가 가장 큰 영향을 주는 것으로 나타났다. 따라서 탐방객의 인식과 만족을 향상시키기 위해서는 공중화장실의 청결한 관리 및 유지, 악취 방지, 소모품의 원활한 공급 등에 우선순위를 두어 집중적으로 관리할 필요가 있는 것으로 나타났다. This study conducts to investigate the relationship between visitors’ perception and satisfaction. Data of 292 visitors were collected from Seongsamjae and Jungsanri in Jirisan National Park. The results showed that maintenance, outlook and design of public toilet are positively related to visitors’ satisfaction. One of the major research findings is that the maintenance of public toilet most affects visitors’ satisfaction. Therefore, the park managers need to put great emphasis on the cleanliness and maintenance, preventing stinks, consumable supplies of public toilet in order to enhance visitors’ perception and satisfaction.

Methanol extract of Myelophycus caespitosus ameliorates oxidative stress‑induced cytotoxicity in C2C12 murine myoblasts via activation of heme oxygenase-1

Cheol Park,Hyun Hwangbo,Han Min Ho,Jin-Woo Jeong,조승목,김기영,황혜진,Yung Hyun Choi 한국수산과학회 2023 Fisheries and Aquatic Sciences Vol.26 No.1

Myelophycus caespitosus, a brown alga belonging to genus Myelophycus, has been traditionally used as a food and medicinal resource in Northeastern Asia. However, few studies have been conducted on its pharmacological activity. In this study, we evaluated whether methanol extract of M. caespitosus (MEMC) could protect against oxidative damage caused by hydrogen peroxide (H2O2) in C2C12 murine myoblasts. Our results revealed that MEMC could suppress H2O2-induced growth inhibition and DNA damage while blocking the production of reactive oxygen species. In H2O2-treated cells, cell cycle progression was halted at the G2/M phase, accompanied by changes in expression of key cell cycle regulators. However, these effects were attenuated by MEMC. In addition, we found that MEMC protected cells from induction of apoptosis associated with mitochondrial impairment caused by H2O2 treatment. Furthermore, MEMC enhanced the phosphorylation of nuclear factor-erythroid-2 related factor 2 (Nrf2) and expression and activity of heme oxygenase-1 (HO-1) in H2O2-treaetd C2C12 myoblasts. However, such anti-apoptotic and cytoprotective effects of MEMC were greatly abolished by HO-1 inhibitor, suggesting that MEMC could increase Nrf2-mediated activity of HO-1 to protect C2C12 myoblasts from oxidative stress.

Fluctuations in Abundance and Species Composition of Fishes that appear in the set net fishery in the Dadohae Marine National Park

Cheong, Hyun-Ho,Park, Kyung-Hee national science museum of korea 2009 Journal of Korean nature Vol.2 No.1

In a joint research of the Korean Biodiversity Information Facility (KBIF), the study analyzed species composition and fluctuations in abundance of fishes caught by the set net fishery installed in the offing of Impo, Dolsando in Yeosu-si, Jeollanamdo in June and September 2008. During the study period, there were 11,180 individuals of 54 species of 39 families in 8 orders that appeared in the set net fishery in the offing of the Dadohae Marine National Park. The numbers of individuals that appeared in June and September were shown in Table 2. Among the fishes caught, the Perciformes was the dominant species with 29 species of 22 families followed by the Tetraodontiformes with 6 species of 2 families and the Pleuronectiforme with 5 species of 4 families. There appeared 3 species of 3 families of the Anguilliformes and the Beloniformes, respectively. There were 4 species of 2 families of the Clupeiformes and 3 species of 2 families of the Scorpaeniformes. There was 1 species of 1 family of the Aulopiformes. During the study period, Scomber japonicus was the dominant species with 9005 individuals followed by Trichiurus lepturus with 1500 individuals and Panpus argenteus with 184 individuals. In June, Trichiurus lepturus was dominant with 1200 individuals followed by Panpus argenteus with 150 individuals and Scomberomorus koreanus with 58 individuals. In October, Scomber japonicus was the dominant species with 9000 individuals followed by Trichiurus lepturus with 300 individuals and Scomberomorus koreanus with 45 individuals. It indicates that the dominance of a species has close relationship with water temperature because fishes migrate to feed in this season. Given the frequency of appearance, Scomber japonicus and Trichiurus lepturus most frequently appeared because they were in the middle of growing into adult fishes while the frequent appearance of Scomberomorus koreanus and Panpus argenteus, which were major species of the fishing industry, contributed to the income of fishermen. There appeared rarely seen species in the offing of Yeosu like Pelamis platurus, Rachycentron canadum (Linnaeus), Lobotes surinamensis, Kyphosus vaigiensis, Abudefduf vaigiensis and Siganus fuscescens (Houttuyn). They are subtropical fishes and seem to move here due to the warmer water affected by the global warming. Additional studies on species composition and fluctuations in abundance of fishes that appear in the set net fishery in the Dadohae Marine National Park will be continuously conducted. So, they will contribute to the increase of fishermen's income by transferring the new fish species to fishermen when newly developed rare fish species are fostered in the lab and evaluated for adaptability and growth potential. The research result will be used as basic data to secure national biological resources, and create/manage biodiversity data by building a biodiversity database network.

태안해안국립공원 지역특화자원을 활용한 탐방프로그램 개발 연구

배준호(Joon-ho Bae),남승민(Seung-min Nam),허학영(Hak-young Heo),송창룡(Chang-ryong Song),박현숙(Hyun-suk Park) 국립공원연구원 2021 국립공원연구지 Vol.12 No.1

본 연구를 위해 먼저 국내·외 해상·해안국립공원의 운영프로그램 현황에 대한 조사를 시행하였으며, 조사된 일부 사례의 경우 기후 및 지형적 요인, 그에 따른 생태환경의 차이, 그리고 관련 법령 차이 등의 이유로 즉시 국립공원에 도입하기 보다는 중·장기적 운영계획과 인프라의 확충, 전문성 확보 등 체계적인 준비가 필요한 것으로 나타났다. 보다 적합한 탐방프로그램의 개발을 위해 사업대상지 현장방문을 실시하였으며, 기존 운영프로그램 현황, 각종 시설, 인력현황 파악, 주변 환경 분석, 담당자 인터뷰 등을 통해 맞춤형 탐방프로그램을 구성하였다. 태안해안국립공원의 경우, 연구원 협업 철새 가락지 부착체험, 태안 해변길 - 솔모랫길 탐방, 태안해변 드론 탐방, 마음의 의자, 연날리기, 새와 예술 그리고 … 등의 체험형 프로그램과 한서대학교와 함께하는 해양 안전 교육 프로그램, 유류 오염 교육 프로그램 등의 교육형 프로그램 등 모두 8가지 해양국립공원 운영프로그램이 도출되었다. 제안된 탐방프로그램을 운영하기 위해서는 별도의 장소 및 운영시설의 확충, 예산편성, 운영을 위한 타당성 검토 등이 우선되어야 할 것이다. 특히 탐방프로그램을 효율적으로 운영하기 위해서는 프로그램 지도자의 역량개발이 필요하며, 현재 운영되고 있는 프로그램을 보완하고 새로운 프로그램을 시행하기 위한 추가적 논의가 필요할 것으로 사료된다. For this study, we first examined the currently operating programs of domestic and overseas maritime and costal national parks. It was found that some of the programs require systematic preparations such as mid- and long-term operation plans, infrastructure expansion, and maintenance of professionalism rather than immediately introducing them into national parks due to the differences in climatic and geographic factors, ecological environment, and relevant regulations. To develop more suitable programs, on-site visits were made and the customized programs were suggested by closely examining the status quo and interviewing people in charge of running the currently offered programs. For Taean Coastal National Park, eight programs were recommended as feasible ones, including the experiential programs such as Bird Ringing, Taean Beach Trail - Sol Sand Trail Walking, Travel Taean Beach with Drone, Thinking Chair, Kite Flying, and Culture & Arts Program (Birds, Arts, and …), and the educational programs such as Marine Safety Training and Oil Pollution Awareness Program. For the suggested programs to be realized, the expansion of separate places and operating facilities for each program, budgeting, and feasibility study should be preceded. To operate the programs effectively and efficiently, in particular, it is necessary to develop the competency of program leaders, and additional discussion for supplement the currently operated programs and implement new programs.

국립공원 내 파충류 자원의 효율적인 관리를 위한 PIT(passive integrated transponder) tag과 무선추적방법(radio telemetry)의 적용

이정현 ( Jung Hyun Lee ),이헌주 ( Heon Joo Lee ),라남용 ( Nam Yong Ra ),김자경 ( Ja Kyeong Kim ),엄준호 ( Jun Ho Eom ),박대식 ( Dae Sik Park ) 한국환경생물학회 2009 환경생물 : 환경생물학회지 Vol.27 No.2

본 연구는 PIT tag (passive integrated transponder)과 무선추적법(radio telemetry)을 오대산국립공원과 월악산국립공원에 서식하고 있는 파충류에 적용한 후, 이를 평가하여 효과적으로 국립공원 내 파충류 자원관리 방안을 모색하기 위하여 수행되었다. PIT tag은 현재까지 알려진 파충류 개체표시법 중에서 가장 효과적인 방법으로 2006년 3월부터 2008년 10월까지 오대산국립공원과 월악산국립공원의 조사지역에 출현한 모든 137마리의 뱀류를 대상으로 적용하였다. 무선추적 연구는 월악산국립공원에서 서식하고 있는 황구렁이(Elaphe schrenckii anomala) 2마리를 대상으로 2007년 9월부터 2008년 11월까지 실시하였다. 연구결과, 두 곳의 국립공원 지역에서 연구기간 동안 17마리가 재 포획되어 평균 12.40%의 재포획률을 보였으며, 재 포획된 개체들의 자료를 통하여 누룩뱀(Elaphe dione)과 쇠살모사(Gloydius ussuriensis) 성체의 SVL 증가를 성공적으로 확인하였다. 무선추적을 통하여 확인된 황구렁이 수컷의 행동권 면적은 MCP=389,600 m2, Kernel 95%=471,800 m2, 암컷은 MCP=162,500 m2, Kernel 95%=208,700 m2인 것으로 확인되었다. 국립공원 내에서 보호와 관리가 필요한 종 혹은 멸종위기대상 종인 파충류를 대상으로 PIT tag과 무선추적을 적용한다면 공원 내 파충류의 효율적인 관리 및 보존방안을 마련하는 데 중요한 기초자료를 제공할 수 있을 것으로 생각된다. To suggest effective ways of the management of reptiles in Korea national parks, we applied PIT (passive integrated transponder) tag and radio telemetry research methods in the monitoring of reptiles in Odaesan and Woraksan national parks. We implanted PIT tags into 137 snakes (six species) captured in both the national parks between March 2006 and October 2008 and also radio-tracked two Korean rat snakes (Elaphe schrenckii anomala) from September 2007 to November 2008 in the Woraksan national park. Of total 137 snakes which we had inserted the PIT tag, 17 snakes (12.40%) were recaptured. Based on the PIT tag data of the recapture, we successfully obtained the annual growth rate of Korean cat snakes (Elaphe dione) and Red-tongue pit-viper (Gloydius ussuriensis). Home range of the Korean rat snakes based on the data of radio-tracking was estimated as 389,600 m2 (MCP: Minimum convex polygon) and 471,800 m2 (Kernel 95%) for males and 162,500m2 and 208,700m2 for females. These results suggest that if we apply PIT tag and radio telemetry research methods to manage reptiles in Korea national parks, it could greatly increase our understanding about their basic ecology and as the result, it could allow us to develop better management and conservation ways of reptiles in Korea national parks.

머리염색이 인체에 미치는 영향

윤형식,황성호,이현륭,김수호,박연석,권낙현,정호진,김동훈,노현주,홍성호,박병찬,이관,정해관 東國大學校醫學硏究所 2002 東國醫學 Vol.9 No.1

일상생활에서 모발염색은 흔히 접할 수 있는 미용의 한 종류로 특히 젊은층을 중심으로 폭발적으로 유행하고 있다. 염색을 위항 사용하는 약제는 표백제와 발색제 등 각종 화학약품이 사용되고 있으나 이로 인한 건강장해에 대한 연구는 그리 많지 않다. 저자들은 염색이 인체의 모발건강에 미치는 영향을 파악하기 위하여 염색과 관련된 주관적 증상과 모발의 변화에 대한 실험적 연구를 시행하였다. 동국대학교 경주 캠퍼스 재학생 80명을 대상으로 설문조사를 시행하여 염색 유 ·무 및 염색 후에 경험한 증상에 대해 설문 조사하였고, 의과대학 재학생 46명을 대상으로 피부 반응 테스트를 실시하였다. 또한 염색 전후의 모발 탄성도를 측정하였고 모발의 상태를 파악하기 위해 전자 현미경검사를 실시하였다. 설문조사 결과 염색 전에 비하여 염색 후 안구혼탁, 안구건조, 시력저하, 발진 및 접촉성 피부염, 모발손상, 모근손상 등의 증상을 더 많이 경험한다고 호소하였다(p<0.05). 모발손상과 모근손상은 헤어드라이어 사용 빈도에 따라 증가하는 것으로 조사되었다(p<0.05). 피부반응검사에서 가려움증이 가장 많은 증상이었으며 이는 여성보다는 남성에서 높은 것으로 조사되었다. 염색 전후의 모발장력은 염색 전 134.5±10.37g, 염색 128.0±30.69g, 염색 이틀 후 112.5±19.69g으로 나타났다. 염색 전후의 모발의 전자현미경 케라틴 층이 현저히 감소하고 모발이 가늘어지는 차이를 보였다. 염색은 모발손상, 모발 케라틴 손상 및 모근 손상, 발진 및 접촉성 피부반응, 안구혼탁, 안구건조, 시력 저하를 유발한다. 따라서 염색약으로 인한 손상에 대한 주의와 예방이 필요하다고 생각한다. 예방대책으로 염색 전 피부테스트를 통한 적합성 여부를 판단하는 것이 필요하며 가급적 염색을 피하는 것이 좋을 것이다. 염색약에 발암물질이 포함되어있다는 보고도 있어 염색 제조사의 철저한 실험과 염색 물질의 선별이 염색으로 인한 부작용을 최소화하는데 중요한 역할을 할 것이다. Hair coloring has became one of the most popular cosmetic activities to younger generations during last decade. However, there are few studies on the health effect of widespread use of chemical dyes. This study was conducted to study the effects of hair coloring dye on hair and other systems. We conducted a questionnaire survey of 80 persons in Kyongju campus, Dongguk University. We have done open patch skin test on 46 medical students. We also conducted scanning electron microscopy to examine the hair strength and structure before and after hair coloring process. Injury of hair and hair bulb, contact dermatitis, turbid eyes, xerophthalmia, and poor visual acuity were the main symptoms complained after hair coloring (p<0.05). Injury of hair and hair bulb were increased by frequency of hair-dryer use(p<0.05). In open patch test, pruritus was complanined by more than half of the subjects. Mean strength of hairs before and after hair coloring was as follows; 134.5 (SD 10.37)g before hair coloring, 128.0 (SD 30.69)g immediately after hair coloring, and 112.5 (SD 19.69)g after two days. The scanning electron microscopic findings of hair surface before and after hair coloring showed decreased keratin layer and thinning of the hair. Hair coloring induces injury to hair, its keratin layer, and hair bulb as well as contact dermatitis, turbid eyes, xerophthalmia, and poor visual acuity. Therefore, we think that precaution is needed in use of hair coloring dye. To prevent complications induced by hair coloring dye, it is necessary, especially to those with allergy or skin disorders, to perform skin test before action and avoid hair coloring whenever possible. Longterm health effects of hairdye should be studied and manufacturing companies should try to minimize complications induced by hair coloring dye.

Morin exerts cytoprotective effects against oxidative stress in C2C12 myoblasts via the upregulation of Nrf2-dependent HO-1 expression and the activation of the ERK pathway

Lee, Moon Hee,Han, Min Ho,Lee, Dae-Sung,Park, Cheol,Hong, Su-Hyun,Kim, Gi-Young,Hong, Sang Hoon,Song, Kyoung Seob,Choi, Il-Whan,Cha, Hee-Jae,Choi, Yung Hyun UNKNOWN 2017 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.39 No.2

<P>In the present study, we investigated the cytoprotective efficacy of morin, a natural flavonoid, against oxidative stress and elucidated the underlying mechanisms in C2C12 myoblasts. Our results indicated that morin treatment prior to hydrogen peroxide (H2O2) exposure significantly increased cell viability and prevented the generation of reactive oxygen species. H2O2-induced comet-like DNA formation and gamma H2AX phosphorylation were also markedly suppressed by morin with a parallel inhibition of apoptosis in C2C12 myoblasts, suggesting that morin prevented H2O2-induced cellular DNA damage. Furthermore, morin markedly enhanced the expression of heme oxygenase-1 (HO-1) associated with the induction and phosphorylation of nuclear factor-erythroid 2-related factor 2 (Nrf2) and the inhibition of Kelch-like ECH-associated protein 1 (Keapl) expression. Notably, these events were eliminated by transient transfection with Nrf2-specific small interfering RNA. Additional experiments demonstrated that the activation of the Nrf2/HO-1 pathway by morin was mediated by the extracellular signal-regulated kinase (ERK) signaling cascade. This phenomenon was confirmed with suppressed Nrf2 phosphorylation and consequently diminished HO-1 expression in cells treated with a pharmacological inhibitor of ERK. Collectively, these results demonstrated that morin augments the cellular antioxidant defense capacity through the activation of Nrf2/HO-1 signaling, which involves the activation of the ERK pathway, thereby protecting C2C12 myoblasts from H2O2,-induced oxidative cytotoxicity.</P>

연잎, 연자육, 연자방 에탄올 추출물의 항염증 활성

이은주(Eun-Joo Lee),서유미(Yu-Mi Seo),김용현(Yong-Hyun Kim),정정욱(Chungwook Chung),성화정(Hwa-Jung Sung),손호용(Ho-Yong Sohn),박종이(Jong-Yi Park),김종식(Jong-Sik Kim) 한국생명과학회 2019 생명과학회지 Vol.29 No.4

연은 아시아 국가에서 음식과 약재로 널리 사용되는 재료이다. 본 연구에서는 연의 잎(leaf, NL), 자육(seed, NS), 자방(seedpod, NSP)으로부터 에탄올 추출물을 제조하고 이들의 항염증 활성과 작용기전을 규명하였다. 이들의 항염증 활성을 연구하기 위하여 LPS로 자극된 RAW 264.7 세포에서 nitric oxide (NO) 생산을 측정하였다. NL, NS, NSP는 세포 생존율에 영향을 주지 않으면서, 농도의존적으로 NO의 생산을 현저하게 저해하였으며, iNOS 및 COX-2와 같은 pro-inflammatory 중재자들의 단백질 발현을 감소시켰다. 또한, NL, NS, NSP는 MAPKs 단백질의 인신화를 감소시키고 NF-κB p65의 핵으로의 이동을 저해함으로써, 세 추출물에 의한 항염증 활성은 MAPKs 경로와 NF-κB 경로를 조절함으로써 이루어짐을 제시한다. 게다가, ROS의 생성이 세 추출물에 의해서 모두 저해되었으며, HO-1의 발현과 HO-1의 전사조절인자인 Nrf2의 핵으로의 이동이 증가되었다. 결론적으로, 이러한 연구결과는 연의 다양한 부위의 추출물인 NL, NS 그리고 NSP는 항염증 활성을 가지고 있으며, MAPKs, NF-κB, Nrf2/HO-1 등 다양한 신호경로를 통해 조절할 수 있음을 제시한다. Nelumbo nucifera, also known as sacred lotus, has mainly been used as a food throughout the Asian countries. In the present study, we prepared ethanol extracts from leaf (NL), seed (NS), and seedpod (NSP) of Nelumbo nucifera and investigated their anti-inflammatory activities in mouse macrophage RAW 264.7 cells. To evaluate the anti-inflammatory activities of NL, NS, and NSP, nitric oxide (NO) production was measured in LPS-stimulated RAW 264.7 cells. NL, NS, and NSP significantly reduced NO production in a dose-dependent manner without affecting cell viabilities. NL, NS, and NSP dramatically decreased the protein expression of pro-inflammatory genes such as iNOS and COX-2. NL, NS, and NSP also suppressed phosphorylation of MAPKs and the nuclear translocation of NF-κB p65 indicating they have their anti-inflammatory activities via regulating mitogen-activated protein kinases (MAPKs) and nuclear factor kappa B (NF-κB) pathways. In addition, we analyzed the production of reactive oxygen species (ROS) by the treatment of NL, NS, and NSP. All extracts reduced ROS production in a dose-dependent manner. And also, they increased heme oxygenase-1 (HO-1) protein expression and the nuclear translocation of nuclear respiratory factor 2 (Nrf2). In conclusion, our results suggest that Nelumbo nucifera has its anti-inflammatory activity via regulating MAPKs, NF-κB, and Nrf2/HO-1 pathways.