Disorders of Small and Large Intestine : ILK (Integrin Linked Kinase) And Beta-Catenin Expression In Colon Polyps

( Hiun Suk Chae ),( Hyung Keun Kim ),( Jin Soo Kim ),( Hye Sook Son ),( Yong Wan Park ),( Eun Deuck Chang ),( Hye Kyung Lee ) 대한소화기학회 2007 SIDDS Vol.9 No.-

Background/Aims: Signaling pathways via ILK and beta-catenin are important ones in cancer progression and apoptosis of various cancer including colon and stomach. It roles of cancer cell are cell to adhesion, cell migration and motility. In colon cancer, two molecules are highly expressed. So we investigated their expression in colon polyps according to gross morphology and pathologic type. Methods: The expression of ILK and beta-catenin are measured with immunohistochemistry (Santa Cruz Co.) According to morphology, we calssified polyps into pedunculated polyps (Ip, 19), sessile polyps (Is, 31) and laterally spreading tumor (LST, 26). In pathologic type, colon polyps classified into adenocarcinoma (2), tubular adenoma (TA, 47), hyperplastic polyp (HP, 22), tubulovillous polyp (TV, 4), villous type (1). Results: According to morphology, there was no significant difference of ILK and beta-catenin expression among Ip, Is and LST (P>0.05). 2 malignant polyps revealed high expression of ILK and beta-catenin. ILK and beta-catenin expression in tubular adenomatous polyps were significantly higher than hyperplastic polyps (P<0.05). And in tubular adenoma, ILK and beta-catenin were highly expressed in high grade dysplasia (P<0.05). Conclusions: ILK and beta-catenin are not different in gross morphology of colon polyps but more expressed in tubular adenoma, especially in severe dysplasia.

Acute appendicitis caused by colonoscopy.

Chae, Hiun-Suk,Jeon, Su-Yun,Nam, Woo-Seok,Kim, Hyung-Keun,Kim, Jin-Soo,Kim, Jeong-Soo,An, Chang-Hyeok 대한내과학회 2007 The Korean Journal of Internal Medicine Vol.22 No.4

<P>A 48-year-old woman who was without any abnormal past medical history underwent colonoscopy as a screening procedure for colorectal disease. The procedure was uneventful and there was no sign of inflammation around the appendicular orifice or the luminal surface of the cecum. The patient did not complain of pain or significant discomfort throughout the procedure. She then developed pain in the right lower quadrant of the abdomen that evening and this persisted for four days. She visited the outpatient department and underwent abdominal ultrasonography, which showed a swollen appendix with a collection of pericecal fluid. Surgical exploration and appendectomy were performed; the final diagnosis was acute suppurative appendicitis. Colonoscopists should be aware of this rare complication and consider it when making the differential diagnosis of post-colonoscopy abdominal pain.</P>

Helicobacter pylori에 감염된 십이지장궤양과 비궤양성 소화불량에서 ABO 혈액형과 HLA의 연관

채현석(Hiun Suk Chae),김태규(Tai Gyu Kim),한훈(Hoon Han),김성수(Sung Soo Kim),최규용(Kyu Yong Choi),정인식(In Sik Chung),차상복(Sang Bok Cha),박두호(Doo Ho Park),김부성(Boo Sung Kim) 대한소화기학회 1996 대한소화기학회지 Vol.28 No.5

N/A Background/Aims: It has been known that genetic factors, for example, blood group, non-secretor and HLA system, are associated with duodenal ulcer and that Helicobacter pylori infection is the major cause of peptic ulcer. However, Helicobacter pylori is also found in non-ulcer dyspepsia and asyrnptomatic patients without ulcer formation. But, it is still not known regarding what kind of genetic factors have an effect on ulcer formation at the time of Helicobacter pylori infection. This study was performed to make clear wbich genetic factors are re1ated with duodenal ulcers among Koreans, and what kind of genetic factors could influence on the ulcer formation in the patients with Helicobacter pylori infection according to ABO blood groups and HLA antigen.'.. Methods: The duodenal ulcer patients (36), non-ulcer dyspepsia (19) and norraal healthy controls (103) were included in this study. Helicobacter pylori infection was detected with phenol red spray method in vivo which was confirmed with Warthin-Starry silver stain. HLA antigen expression (HLA-A,B) of peripheral blood T lymphocytes was studied with microlymphocytotoxicity teclmique. Results: Tbe frequency of HLA-A 33 was higher in duodenal ulcer patients (l4/36, 38.9%) compared with the control group (21/103, 20.4%). On the contrary, no difference in HLA-B has been shown between duodenal ulcer patients and controls. Among patients with Helicobacter pylori infection, blood group 0 was significant1y more frequent in patients with duodenal ulcers (21/36, 58.3%) than in non-ulcer dyspepsia (5/19, 26.3%). In patients with HLA-A 33, blood group 0 was significantly more frequent in duodenal ulcer patients (7/)4, 50%) than in non-ulcer dyspepsia patients (0/7, 0%). Conclusions: In Helicobacter pylori-infected patients, HLA-A 33 is related with duodenal ulcers and the patients with both blood group 0 and HI.A-A 33 are more likely to have duodeual ulcers than those with HLA-A 33 and without blood groop O. (Korean J Gastrnenterol 1996; 28:623 - 631)

신장 ; 대장용종에서 ILK (Integrin-Linked kinase)와 beta-catenin의 발현

채현석 ( Hiun Suk Chae ),김진수 ( Jin Soo Kim ),손혜숙 ( Hye Sook Sohn ),노상영 ( Sang Young Rho ),김형근 ( Hyung Keun Kim ),조영석 ( Young Suk Cho ),이보인 ( Bo In Lee ),강진형 ( Jin Hyung Kang ),최규용 ( Kyu Yong Choi ),맹이소 ( 대한내과학회 2009 대한내과학회지 Vol.77 No.3

목적: ILK와 beta-catenin을 통한 세포전달은 여러 악성 질환의 시작과 진행에 있어서 중요하다. ILK는 beta-catenin의 전사를 조절하며 암세포에서 세포 이동 및 침습에 관여한다. 최근에 대장암과 전구 단계인 샘종에서 ILK와 beta-catenin이 발현한다는 연구가 있었다. 이에 저자들은 대장용종에서 육안적 및 병리학적 소견의 차이에 따른 ILK와 beta-catenin의 발현을 알아보았다. 방법: 비악성 질환으로 수술한 6명의 정상 대장 조직(6)과 대장용종절제술로 획득한 용종을 모양에 따라 유경성 용종(Ip, 16), 무경성 용종(Is, 22) 및 측방 발육형 종양(laterally spreading tumor LST, 24)으로 나누었다. 또한 현미경적 소견에 따라 관샘종(TA, 47)과 과형성 용종(HP, 15)으로 나누고 관샘종은 이형성의 정도에 따라 나누었다. 이들 조직에서 ILK와 beta-catenin의 발현을 면역화학염색법으로 비교하였다. 결과: 용종의 육안적 모양에 따라 Ip, Is, LST에서 ILK, beta-catenin의 발현의 차이는 통계적으로 차이가 없었다(p>0.05). 조직학적으로 ILK, beta-catenin의 발현은 과형성 용종보다 관상 샘종에서 발현이 증가하였다(p<0.05). 관상 샘종에서는 이형성의 정도가 심할수록 ILK와 beta-catenin의 발현이 높았다(p<0.05). 전체 용종에서 ILK의 발현이 높은 용종의 경우에 beta-catenin의 발현이 높은 경우가 많았다(p<0.01). 결론: 대장용종에서 ILK와 beta-catenin의 발현은 대장용종의 모양에 따른 차이가 없었고, 샘종이 과형성 용종보다 발현이 증가되었고 샘종의 이형성이 심할수록 발현율이 높았다. Background/Aims: Signaling pathways via integrin-linked kinase (ILK) and β-catenin are important in the initiation and progression of various malignant diseases. ILK modulates the transcription of β-catenin and is implicated in cell migration and invasiveness. Recently, premalignant colon polyps were found to express ILK and β-catenin. Therefore, we investigated the expression of ILK and β-catenin in colon polyps according to the gross morphology and pathologic type. Methods: Based on morphology, colon polyps (62) were classified as being a pedunculated polyp (Ip, 16), sessile polyp (Is, 22), or laterally spreading tumor (LST, 24). The colon polyps were classified pathologically as tubular adenomas (TAs, 47) and hyperplastic polyps (HPs, 15). The expression levels of ILK and β-catenin in colon polyps and normal colon (6) were evaluated with immunohistochemistry. Results: In normal colon, ILK was not expressed, and β-catenin stained in the cell membrane only. Based on the gross morphology of the colon polyps, no significant difference was seen in the expression of ILK and β-catenin (p>0.05). The expression of both ILK and β-catenin in TAs was greater than that in HPs (p<0.01): the greater the dysplasia in TAs, the more both ILK and β-catenin were expressed (p<0.05). The grade of expression of ILK was correlated with that of β-catenin in colon polyps (p<0.01). Conclusions: The expression of ILK and β-catenin did not differ according to the morphology of colon polyps, but was expressed more in TAs than in HPs, especially in severe dysplasia. (Korean J Med 77:315-320, 2009)

구연 / 제 2 회의장 / 위장관 39 : 흰쥐의 대장에서 Dimethylhydrazine 에 의해 유발된 aberrant crypt foci 의 부위와 시간에 따른 변화

채현석(Hiun Suk Chae),오수혁(Soo Hyun Oh),이봉수(Bong Soo Lee),한석원(S . W . Han),이창돈(Chang Don Lee),최규용(K . Y . Choi),정인식(I . S . Chung),선희식(H . S . Sun),맹이소(Lee so Maeng),이은정(Een Jung Lee) 대한소화기학회 1999 대한소화기학회 추계학술대회 Vol.1999 No.-

소화기 ; 복벽에서 기원한 복통 환자의 평가

채현석 ( Hiun Suk Chae ),문도준 ( Do Jun Moon ),김윤태 ( Yoon Tae Kim ),이정수 ( Jung Soo Lee ) 대한내과학회 2009 대한내과학회지 Vol.76 No.5

목적: 소화기내과에서 재활의학과로 의뢰된 원인불명의 복통을 갖고 있는 환자의 특성을 조사하고 그 중 복벽 기원통증을 갖고 있는 환자의 빈도 및 특성을 분석하여 추후 복벽 기원 통증을 갖고 있는 환자를 진단하고 치료하는데 도움이 되고자 하였다. 방법: 2003년 8월 1일부터 2007년 8월 31일까지의 기간 동안 소화기내과를 방문한 10,586명의 환자 중 의무기록을 통한 후향적 연구방법을 이용하여 내과적, 방사선적, 내시경적 복강 내 원인이 없고 복벽 기원 통증이 의심되어 재활의학과로 협진 의뢰된 185명을 조사하였으며, 그 중 실제로 재활의학과를 방문한 100명(남자 37명, 여자 63명)을 대상으로 하였다. 복통의 부위는 9개의 구획으로 나누어서 분류하였으며 복벽 기원 통증의 특성, 부위, 증상기간, 원인, 치료 및 치료 효과 등을 분석하였다. 또한 전화 통화를 통하여 장기간의 치료효과 및 환자 상태를 평가하였다. 결과: 복벽통증이 의심되어 소화기내과에서 재활의학과로 의뢰된 100명 중 89%에서 복벽 기원 통증으로 진단되었으며 그 중 우요부 통증이 가장 많았다. 복벽 기원 통증 중근육골격계 원인이 90.4%, 신경성 원인이 9.6%였다. 그 중 재활의학과적 치료로 효과가 있는 경우가 73%였으며 추적관찰한 결과 95.5%에서 처음에 내린 진단명이 변하지 않았다. 복벽통증 환자 중 장기간 치료 효과가 유지되었던 경우는 73.6%였다. 결론: 복통 환자가 외래를 방문했을 때 우선 복강 내 원인을 철저히 조사해야 하며, 복강 내 원인을 찾지 못했을 경우라도 정신신체장애나 기능성 장질환으로 진단하기에 앞서 복벽 기원 통증을 염두에 두어야 하며 이를 위해서는 좀더 체계적인 진단적 및 치료적 도구를 확립하는 것이 필요하다. 복벽 기원 통증을 잘 이해하면 일부 그룹에서는 재활의학과적 접근 및 치료가 상당히 도움이 될 수 있으며 환자의 시간적 및 경제적인 부담도 더욱 경감하게 되어 결과적으로 환자의 삶의 질도 더욱 향상시킬 수 있을 것으로 생각한다. 이때 주의해야 할 점은 복벽 기원 통증으로 진단되더라도 복강 내 원인도 항상 염두에 두어야 한다는 점이며 그 이후에도 환자의 추적관찰 및 관리가 필요하다는 것이다. Background/Aims: We analyzed patients with abdominal pain of undetermined etiology, referred to physiatrists by gastroenterologists, and determined the clinical features of patients with abdominal wall pain. Methods: A retrospective chart review of 100 patients referred by gastroenterologists over 4 years was performed. A visceral etiology of abdominal pain was excluded by the gastroenterology work-up using radiologic and endoscopic studies, and medical treatment. The clinical features and outcomes of abdominal wall pain were examined. We determined the long-term outcome by telephone. Results: Of 100 patients with abdominal pain of undetermined etiology, 89% of the patients were identified as having pain arising in the abdominal wall, and the right flank area was the most common site of pain. The pain had a musculoskeletal origin in 90.4% of the patients and a neurogenic origin in 9.6%. Treatment by the physiatrists resulted in complete or partial pain relief in 73% of the patients. The diagnosis remained unchanged after a mean of 22.67 (range 6.5-55) months in 95.5% of the patients. Long-term effects of management were seen in 73.6% of the patients. Conclusions: In a small group of patients with abdominal pain of unknown etiology, the source of pain could be in the abdominal wall. Recognizing abdominal wall pain could lead to an accurate diagnosis, reduced medical costs and effective treatment. (Korean J Med 76:564-570, 2009)

Effect of Eupatilin on Cell Viability and Lytic Gene Expression in Epstein -Barr Virus Positive Gastric Carcinoma Cells

LEE Suk Kyeong,CHAE Hiun Suk,OH Tae-Young,SEO Jung Seon 大韓藥學會 2005 大韓藥學會 總會 및 學術大會 Vol.2005 No.2

The Korean Society of Gastroenterology & SIDDS 2033 : Slide Session ; S-LG-06 : Lower GI Tract ; Long-Term Clinical Outcomes of Urban Versus Rural Environment in Korean Patients with Crohn`s Disease: Results from the Connect Study

( Yoon Suk Jung ),( Dong Il Park ),( Byong Duk Ye ),( Jae Hee Cheon ),( You Sun Kim ),( Young Ho Kim ),( Joo Sung Kim ),( Hiun Suk Chae ),( Kwang Ho Baik ),( Dong Soo Han ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: Environmental factors and genetic predisposition are thought to play an important role in the pathogenesis of Crohn`s disease (CD). Although numerous studies have reported the positive association between urban environment and CD development, few studies have compared the clinical outcomes between urban and rural environments. Therefore, this study aimed to compare the clinical characteristics and long-term prognosis between urban and rural populations of patients with CD. Methods: This retrospective multicenter cohort study included 1002 Korean patients with CD (743 urban residents and 259 rural residents) from 32 medical centers. The clinical outcomes of urban versus rural populations were compared using the Kaplan-Meier method and log-rank test. Results: Disease distribution and behavior of the urban population did not differ from those of the rural population. There were no signifi cant differences in the cumulative probabilities of perianal fi stula (P=0.086) and intestinal complications such as stricture (P=0.109), fi stula (P=0.952), abscess (P=0.227), and perforation (P=0.382) between the two groups.In addition, no signifi cant differences were observed between the two groups with regard to the cumulative probabilities of immunosuppressant use (P=0.527) and biological agent use (P=0.731). However, the cumulative probability of surgery in the urban population was signifi cantly higher than that in the rural population (19.2%, 25.8%, and 35.8% vs. 13.6%, 20.4%, and 29.8% at 1 year, 5 years, and 10 years, respectively; P=0.040). Conclusions: The urban population required surgical treatment more frequently than the rural population, although there were no significant differences in cumulative probabilities of immunosuppressant and biological agent use. Our results suggest that factors associated with an urban lifestyle may infi uence poor prognosis of CD.

Combined Hepatic and Splenic Abscesses in a Patient withUlcerative Colitis

김성수,Hiun-Suk Chae,Jeong-Seon Ji,Bo-In Lee,Hwang Choi,Byung-Wook Kim,Kyu-Yong Choi,Hyung-Keun Kim,Chang-Wook Kim,조영석 대한의학회 2007 Journal of Korean medical science Vol.22 No.4

Expression of Viral MicroRNAs in Epstein-Barr Virus-Associated Gastric Carcinoma

Kim, Do Nyun,Chae, Hiun-Suk,Oh, Sang Taek,Kang, Jin-Hyoung,Park, Cho Hyun,Park, Won Sang,Takada, Kenzo,Lee, Jae Myun,Lee, Won-Keun,Lee, Suk Kyeong American Society for Microbiology 2007 Journal of virology Vol.81 No.2

<B>ABSTRACT</B><P>Epstein-Barr virus (EBV) is associated with about 6 to 16% of gastric carcinoma cases worldwide. Expression of the EBV microRNAs (miRNAs) was observed in B cells and nasopharyngeal carcinoma cells infected with EBV. However, it is not clear if the EBV miRNAs are expressed in EBV-associated gastric carcinomas (EBVaGCs). We found that BART miRNAs but not BHRF1 miRNAs were expressed in EBV-infected gastric carcinoma cell lines and the tumor tissues from patients as well as the animal model. The expression of viral miRNAs in EBVaGCs suggests that these EBV miRNAs may play important roles in the tumorigenesis of EBVaGCs.</P>