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Crystal Structure and Morphology of Poly(16-hexadecalactone) Chain-folded Lamellar Crystals
Kim, Eunju,Uyama, Hiroshi,Doi, Yoshiharu,Ha, Chang-Sik,Iwata, Tadahisa WILEY-VCH Verlag 2005 Macromolecular bioscience Vol.5 No.8
<P>Summary: Solution-grown, chain-folded lamellar crystals of poly(16-hexadecalactone) (PHDL) were crystallized isothermally from 1-hexanol at 70 °C. The morphology of lozenge-shaped crystals was studied by TEM and AFM. The lamellae are ca. 10 nm thick and the chains run orthogonal to the lamellar surface with folding along (110) and (<TEX>$1\bar 10$</TEX>) planes. The crystal structure of PHDL was determined by XRD and election diffraction of single crystals. The chains are in the 2<SUB>1</SUB> helix conformation close to all-trans and the structure consists of an orthorhombic unit cell with a P2<SUB>1</SUB>2<SUB>1</SUB>2<SUB>1</SUB> space group with the lattice constants a = 0.746 ± 0.001 nm, b = 0.504 ± 0.001 nm, and c (chain axis) = 4.116 ± 0.003 nm. There are two chains per unit cell, which exist in an antiparallel arrangement. Molecular packing structure has been studied in detail, taking into account both diffraction data and energy calculations. The setting angles, with respect to a axis, were ±40° for the corner and center chains, respectively. By using the electron and XRD data, the best molecular packing model was refined to R-factors of 0.168 and 0.196, respectively. A brief comparison of chain-packing structure is also made with related polymer structures.</P><P> <img src='wiley_img/16165187-2005-5-8-MABI200500038-gra001.gif' alt='wiley_img/16165187-2005-5-8-MABI200500038-gra001'> Graphic </P>
Promotion Effects of Ultra-High Molecular Weight Poly-γ-Glutamic Acid on Wound Healing
Choi, Jae-Chul,Uyama, Hiroshi,Lee, Chul-Hoon,Sung, Moon-Hee The Korean Society for Microbiology and Biotechnol 2015 Journal of microbiology and biotechnology Vol.25 No.6
We examined the in vivo efficacy of ultra-high molecular weight poly-γ-glutamic acid (UHMW γ-PGA) for wound healing. The wound area was measured by a ruler and documented by digital photography before the animals were sacrificed at days 8 and 16 post wounding. The areas of wounds treated with UHMW γ-PGA were significantly decreased on days 8 and 16, as compared with those receiving a control treatment, and more than 70% of the UHMW γ-PGAtreated area was repaired by day 8. Hematoxylin and eosin staining confirmed that the epidermis had regenerated in the UHMW γ-PGA-treated wounds. At 16 days post wounding, collagen pigmentation and cross-linking were increased as compared with the control groups, and greater regeneration of blood vessels had occurred in UHMW γ-PGA-treated groups. Increased levels of transforming growth factor-beta and β-catenin were also observed in skin samples collected from UHMW γ-PGA-treated animals on days 8 and 16 post incision. Taken together, these findings suggest that UHMW γ-PGA promotes wound healing in vivo.
Ji-Hye Lee,Hiroshi Uyama,권오경,김영진 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.94 No.-
Photothermal therapy (PTT) has been extensively explored as a promising alternative therapeuticapproach for many malignant tumors. However, PTT agents never repair bone defects because of theirlack of osteogenic activity. Therefore, the integration of multi-modal therapies into one platform couldshow great promise in overcoming the drawbacks of conventional single-modal therapy and achievingimproved therapeutic efficacy in cancer. In this study, an indocyanine green (ICG) and diethylenetri-amine/nitric oxide adduct (DETA/NO)-loaded polylactic acid (PLA) (PLIN) monolith was created viasimple thermally induced phase separation (TIPS) and used to enhance PTT efficacy by killing residualcancer cells and regenerating bone defects after surgical resection of tumor tissues. The resulting PLINmonolith exhibited interconnected microporous structures and uniform morphology composed of leaflikesmall units. The results of in vitro PTT efficacy tests demonstrated that the PLIN monolith exhibitedenhanced photothermal activity on osteosarcomas but did not caused phototoxicity in normalosteoblasts. The synergistic cancer therapy efficacy of PLIN was mainly caused by high local temperatureand elevated intracellular generation of ONOO in MG63 cells. In addition, PLIN induced significantapoptotic cell death following laser irradiation. Therefore, the PLIN monolith has potential for applicationin osteosarcoma therapy and bone regeneration.
( Jae Chul Choi ),( Hiroshi Uyama ),( Chul Hoon Lee ),( Moon Hee Sung ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.3
We investigated the effect of ultra-high molecular weight poly-γ-glutamic acid (UHMW γ-PGA) on hair loss in vitro and in vivo. 5-Alpha reductase is an enzyme that metabolizes the male hormone testosterone into dihydrotestosterone. By performing an in vitro experiment to analyze the inhibitory effects of UHMW γ-PGA on 5-alpha reductase activity, we determined that UHMW γ-PGA did in fact inhibit 5-alpha reductase activity, indicating the use of UHMW γ-PGA as a potential 5-alpha reductase inhibitor in the treatment of men with androgenetic alopecia. To evaluate the promotion of hair growth in vivo, we topically applied UHMW γ-PGA and minoxidil on the shaved dorsal skin of telogenic C57BL/6 mice for 4 weeks. At 4 weeks, the groups treated with UHMW γ-PGA showed hair growth on more than 50% of the shaved skin, whereas the control group showed less hair growth. To investigate the progression of hair follicles in the hair cycle, hematoxylin and eosin staining was performed. Histological observations revealed that the appearance of hair follicles was earlier in the UHMW γ-PGA-treated group than in the control group. The number of hair follicles on the relative area of shaved skin in the UHMW γ-PGA-treated group was higher than that observed on the shaved skin in the control group. These results indicate that UHMW γ-PGA can promote hair growth by effectively inducing the anagen phase in telogenic C57BL/6 mice.
Optimization of Correction Factor for Linearization with Tc-99m HM PAO and Tc-99m ECD Brain SPECT
Won, Kyu Chang,Watabe, Hiroshi,Kume, Norihiko,Lee, Hyoung Woo,Hayashida, Kohei,Cho, Ihn Ho,Uyama, Chikao 영남대학교 의과대학 1999 Yeungnam University Journal of Medicine Vol.16 No.2
연구목적: ^(99m)Tc d,l-hexamethylpropyleneamine oxime(HMPAO)와 ^99mTc ethyl cysteinate dimer(ECD)의 뇌세포에 의한 섭취는 뇌혈류량에 비례를 한다. 그러나 뇌혈류량이 아주 높은 경우에는 뇌세포에 의한 섭취가 그 만큼 증가를 하지 않기 때문에 뇌혈류량이 과소평가 될 수 있다. 이를 보안하기 위하여 Lassen이 linearization algorithm을 만들었다. 그러나 이 방정식은 뇌의 상태에 따라 사용되는 알파값으로 표현되는 변수가 변할 수 있다. 저자들은 뇌경색이 있는 10명의 환자를 대상으로 가장 적절한 α값을 구하고자 하였다. 재료 및 방법: 10명의 환자들은 모두 0.1에서 10까지의 알파값을 이용하여 교정한 ^99mTc-HMPAO와 ^(99m)Tc ECD 뇌관류 단일광자방출 단층촬영(single photon emission computed tomography: SPECT) 영상을 구하고, 양전자방출단층촬영술로 뇌혈류영상을 구하였다. 그리고 상호정보의 최대화에 의한 multi-modal volume registration을 이용하는 컴퓨터프로그램으로 양전자방출단층촬영술로 구한 뇌혈류와 SPECT 영상에서 다양한 알파값을 대입하여 Lassen의 linearization algorithm으로 구한 뇌혈류값을 픽셀단위로 서로 비교하였다. 결과: Lassen의 linearization algorithm을 이용하여 구한 ^(99m)TC-HMPAO와 ^99mTC-ECD 뇌관류 SPECT의 국소 뇌혈류량은 알파값이 각각 1.4와 2.1일때 양전자방출전산화단층촬영술로 구한 뇌혈류량과의 상관관계가 가장 높았다. 결론: Lassen의 linearization algorithm을 이용하여 뇌혈류량을 정량화하는 경우에는 ^99mTC-HMPAO의 경우는 1.4, ^(99m)TC-ECD의 경우는 2.1을 사용할 때 뇌혈류량을 가장 잘 반영할 것으로 생각된다.