균류 분화과정의 유전적 조절기작

박범찬,이환희,박윤희,박희문 충남대학교 생물공학연구소 2003 생물공학연구지 Vol.9 No.1

Fungi are well suited for studying mechanism controlling development and cell differentiation in multicellular eukaryotes. The asexual and sexual reproductive cycles are tightly coordinated during the life cycle of Aspergillus nidulans. The asexual (mitotic) reproductive cycle involves the formation of a number of differentiated cell types. Successful formation of multicellular reproductive structures and spores (conidia) requires the establishment of spatiotemporal gradients in essential transcriptional regulators, such as brlA, abaA and wetA. Altered gene expression is coordinated with critical changes in the movement of nuclei and the regulation of the cell division cycle. Therefore, it has been proposed to define a central regulatory pathway. Several early regulatory genes involved in signal transduction pathway controlling asexual sporulation are also required for activating the central pathway. Sexual (meiotic) reproduction is more complex, requiring the differentiation of several specialized tissue types. Although technical difficulties have limited efforts to study the genetics of sexual fruiting-body formation directly, some recent progress has been made. With the recent availability of mutants blocked in sexual development, understanding the genetic interactions between genes that function primarily in determining sexual sporulation will be achieved in the near future.

간호실무에서 신봉이론(Espoused theories)에 대한 탐색적 연구

서문자,김혜숙,이은희,박영숙,조경숙,강현숙,임난영,김주현,이소우,조복희,이명하,지성애,하양숙,손영희,권성복,김희진,추진아 성인간호학회 2001 성인간호학회지 Vol.13 No.1

As a nursing practice involves nurses' actions in a specific context of health care, this study has focused on exploring the espoused theories in nursing practice within the action science perspectives, Espoused theories are the belief, principles, and rationale expressed by the practitioner as guiding her/his actions in a situation of practice. The data were analysed qualitatively and 25 elements of espoused theories of nursing action were identified and clustered into 6 categories. The 25 elements of espoused theories are as follows: The clinical nurse worked in wholistic and individual nursing, focussed on the patient's needed, comfort and supportive nursing (5 theories of nursing goal) ; excellent skills, knowledge based, assessment and data collection, explaining, educating or a scientific basis(6 theories of nursing intervention): advocacy, value oriented, treatment, account- ability and commitment(4 theories of nursing ethics) ; human respect. partnership, trust(3 theories of patient-nurse relationship) : knowledgable, accumulated clinical experiences and personally lived experiences. positive perspectives(4 theories of nurse), role of intervention. rewarding peer relationship(3 theories of situations) The above mentioned espoused theories are similar to that of nursing textbooks which students learned through basic nursing education and almost the same as the Acts ofa Nurse in Korean. However, we are doubtful whether nurses actually do as they think. Therefore. it is recommended to review the theories-in-use in order to and any discrepancies between the espoused theories and the reality of nursing actions

바이모달 트램의 위치 인식 방법 및 정밀 정차 구현

서기원(Seo Ki-Won),박주연(Park Ju-Yeon),이상남(Lee Sang-Nam),류희문(Ryu Hee-Moon),변윤섭(Byun Yeun-Sub) 한국철도학회 2009 한국철도학회 학술발표대회논문집 Vol.2009 No.5월

This paper presents a method for precisely localizing and parking of bimodal trams. In order to gain an automatically driving system for bimodal trams, precise up-to-date localization, velocity recognition, distance to next station and precise parking location estimation functions are required. This paper proposes a system consisting of control device, steering device, sensor input equipment, driving system, tachometer, vehicle-side sensors, magnetic markers and magnetic sensors. The tram recognizes the precise location via magnetic markers containing information. Parking position and precise distance calculation is embodied by a tachometer. The vehicle-side sensors are used to assure safe station approaching and parking magnetic markers provide improvement of precision while tram parking. This paper provides a system realizing localization and precise parking and afterwards the automatic drive test results are reported and analyzed.

급성 출혈성 대장염으로 오인된 궤양성 대장염 1예

문희정,장병익,김승범,이호찬,박재현,은종렬,김태년 영남대학교 의과대학 2008 Yeungnam University Journal of Medicine Vol.25 No.2

Ulcerative colitis (UC) is a chronic inflammatory disorder of the gastrointestinal tract that affects the large bowel, Its etiology remains controversial. However, an infectious or immunologic origin is considered the primary cause. The onset of UC is typically slow and insidious, but some patients may present acutely with symptoms mimicking infectious colitis. We report a case of ulcerative colitis mimicking acute hemorrhagic colitis at initial presentation. A 60-year-old man was referred to Yeungnam University Hospital for bloody diarrhea and abdominal pain. Sigmoidoscopy revealed mildly edematous mucosa in the rectum and hyperemic mucosa with petechiae in the sigmoid colon. The patient was treated with antibiotics for several days, and his symptoms improved. However, after one month, his bloody diarrhea relapsed. Follow-up sigmoidoscopy revealed mucosal friability in the rectum and sigmoid colon. He was diagnosed with ulcerative colitis, and his symptoms were improved with mesalazine and a steroid enema.

Cloze Test의 결과분석 및 他 성적과의 相關度 조사

朴熙文 培材大學校 1983 論文集 Vol.4 No.-

Recently, many test writers repeatedly point out the possibility of using cloze tests as a reliable means of testing device in ESL/EFL classrooms. The cloze test is merely a passage with blanks inserted for words that have been deleted. Usually every fifth, sixth or seventh word is deleted. The purpose of this paper is to investigate the correlation between cloze tests and other language proficiency tests, and to analyze the relative difficulty of parts of speech to be filled in each blank. The result of the study shows that the cloze test used has a high correlation (0.896) with other language proficiency tests. This implies that cloze tests can be used as an effective tool for measuring students' language proficiency. The result also shows that while relatively good at nouns and verbs, students are particularly poor at the usage of past auxiliaries and definite article, 'the'. Though further study on the validity and cognitive process of cloze tests is definitely needed in depth, it may be safe to conclude that the cloze test is a practical, reliable test that can be easily constructed and used by any ESL/EFL teacher.

영어 전치수식 형용사의 어순규칙 수립을 위한 핵어문법(HPSG)적 접근

박희문 한국현대영어영문학회 2006 현대영어영문학 Vol.50 No.1

Candida albicans의 형태변환 조절과정 규명의 최근 연구 동향

박윤희,박희문 충남대학교 생물공학연구소 2009 생물공학연구지 Vol.15 No.-

The opportunistic fungal pathogen Candida albicans can grow as yeast, pseudohyphae or true hyphae, and morphology of each cell is essential for efficient dissemination and tissue invasion. Therefore the ability to switch in C. albicans is thought to be an important virulence factor. The morphological conversion of C. albicans can be triggered in vitro by a various environmental stimuli including serum, pH, temperature, and nutrient depletion. These inducers regulate the expression of hypha-specific genes, which are involved in morphogenesis, through multiple signaling pathways such as MAPK pathway and cAMP/PKA pathway. In order to investigate Signal transduction pathways, disruption or over-expression of the component gene(s) were performed and then change(s) in morphological transition as well as the expression of hypha-specific genes are observed in general. As the approach to unravel new roles of already-known signaling pathways in fungal pathogenicity, responses toward external stimuli such as hypoxic condition and iron utilization are performed these days. The new approaches revealed that the morphological conversion in C. albicans is resulted from the consorted action of multiple signaling pathways rather than from a single pathway. Consequently, the existence of a novel Signaling pathway for dimorphic switch in C. albicans could not be excluded. In this context, it is noteworthy that the involvement of LAMMER kinase in fungal development; LAMMER kinase is known to be involved in oxidative-stress response, filamentous growth, and flocculation in fission yeast; S. cerevisiae LAMMER kinase, ScKNS1, is also reported to phosphorylate Slt2, MAPK of cell wall integrity pathway, and to interact with dual-specificity phosphatase Sdp1, which regulates the activity of Slt2. Therefore, studies on the function of LAMMER kinase in C. albicans will be helpful to reveal novel regulatory mechanisms for virulence in pathogenic fungi.

Triton X-100이 Trichoderma koningii의 성장 및 형태에 미치는 영향

박희문,민경렴,맹필재,하영칠 충남대학교부설 생명공학연구소 1992 생물공학연구지 Vol.2 No.-

Triton Ⅹ-100이 Trichoderma koningii의 성장 및 형태에 미치는 영향을 조사하기 위하여, Triton Ⅹ-100 첨가 여부에 따른 각 종 성장계수의 변화를 조사하여 보았다. 액체 배양시 Triton Ⅹ-100의 첨가에도 불구하고, specific growth rate과 doubling time은 변화가 없었으나, Triton Ⅹ-100을 첨가한 경우, 군체의 방사상 성장률이 감소하였다. 이러한 현상은 Triton Ⅹ-100에 의하여 균사의 분지 형성이 촉진되고, 격막형성이 촉진되어 격막 간의 간격이 짧아져 나타나는 것으로 확인되었다. We investigated the effect of Triton Ⅹ-100 on the growth and morphology of Trichoderma koningii by comparing various parameters representing the growth of mold in the presence or absence of Triton Ⅹ-100. The specific growth rate and doubling time of T. koningii were not affected by the addition of 0.05% Triton Ⅹ-100 into solid medium also resulted in decrease in the colony radial growth rate and this response was correlated with the formation of mycelia which showed increase in branching and septation in the presence of Triton Ⅹ-100.

보건관리 대행사업 평가 지표 및 척도개발에 관한 연구

박정일,조병희,구정완,김광종,김현욱,원종욱,이명숙,이원철,정문희 가톨릭대학교 산업의학센터 2000 韓國의 産業醫學 Vol.39 No.3

This study was carried out to develop the index and the scales for the evaluation of the group occupational health services(GOHS). The possible variables of each step, firstly, based on an evaluation model composed of four steps, input, throughout, output and outcome, were selected through several meetings of professional committee. And five types of questionnaire(for doctor, nurse, industrial hygienist, employer, and employee) were developed based on the selected variables. A pilot survey for four GOHS institutions and 12 enterprises was conducted using the questionnaires and correlation analysis for extracting variables was done. The results were as follows: 1.The evaluation scale was composed of three areas(evaluation on workers' health and work environment, evaluation by institution and employer, and evaluation by employee)which were devided into ten subscales. 2.Among firstly selected ninety-six variables, forty-three variables (11 for input, 14 for throughout, and 18 for output indices) were extracted by correlation analysis. 3.Based on the extracted variables, the questionnaires were simplified as five items for doctor, thirty-six items for nurse, nine items for industrial hygienist, and twenty-six items for employer and employee. 4.The total score of evaluation model was defined on the basis of four-hundred points as sum of four indices (input, throughout, output and outcome) given one-hundred points each.

義城附近 慶尙系砂岩의 堆積岩石學的 硏究

朴文熙,金珠煥 慶北大學校 自然科學大學 地質學科 地質學硏究會 1977 伏賢地質 Vol.- No.3