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Poster Session 2 : Transduction of human Grb7 mediated by HIV-1 Tat basic domain into skin cells
( So Young Kim ),( Jae Jin An ),( Dae Won Kim ),( Soo Hyun Choi ),( Hee Soon Choi ),( Sun Hwa Lee ),( Jin Seu Park ),( Sang Chul Lee ),( Har Young Poo ),( Hyung Joo Kwon ),( Tae Yoon Kim ),( Won Sik E 한국생화학분자생물학회 (구 한국생화학회) 2005 생화학분자생물학회 춘계학술발표논문집 Vol.2005 No.-
Lee, Young Ik,Poo, Har Young,Robert F. Todd III,Petty, Howard R . 생화학분자생물학회 1999 BMB Reports Vol.31 No.1
Recent studies have suggested that integrin (CR3) participates in the signal transduction pathways of certain GPI-anchored phagocytic receptors including FcγRIIIB. One consequence of this functional linkage is an inducible association between CR3 and cortical microfilaments that is triggered by FcγRIIIB binding to immobilized immune complexes (IC). That this signaling event requires the co-expression of FcγRIIIB with CR3 was documented by the use of NIH 3T3 transfectants expressing both CR3 and FcγRIIIB (clone 3-23), CR3 alone (clone 3-19), and FcγRIIIB alone (clone 3-15). Pretreatment of 3-23 cells with protein kinase inhibitors such as staurosporine and methyl 2,5-dihydroxycinnamate (MDHC) blocked IC-stimulated CR3-microfilament proximity without affecting the extent to which FcγRIIIB constrains the lateral membrane mobility of a subset of CR3 on the cell surface (as measured in fluorescence recovery after photobleaching experiments). These data support that CR3 and FcγRIIIB molecules are physically and functionally associated and that ligation of FcgRIIIB triggers CR3-dependent signal transduction.
Bio-Derived Poly(γ -Glutamic Acid) Nanogels as Controlled Anticancer Drug Delivery Carriers
( Hee Ho Bae ),( Mi Young Cho ),( Ji Hyeon Hong ),( Har Young Poo ),( Moon Hee Sung ),( Yong Taik Lim ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.12
We have developed a novel type of polymer nanogel loaded with anticancer drug based on bio-derived poly(γ- glutamic acid) (γ-PGA). γ-PGA is a highly anionic polymer that is synthesized naturally by microbial species, most prominently in various bacilli, and has been shown to have excellent biocompatibility. Thiolated γ-PGA was synthesized by covalent coupling between the carboxyl groups of γ-PGA and the primary amine group of cysteamine. Doxorubicin (Dox)-loaded γ-PGA nanogels were fabricated using the following steps: (1) an ionic nanocomplex was formed between thiolated γ-PGA as the negative charge component, and Dox as the positive charge component; (2) addition of poly(ethylene glycol) (PEG) induced hydrogen-bond interactions between thiol groups of thiolated γ-PGA and hydroxyl groups of PEG, resulting in the nanocomplex; and (3) disulfide crosslinked γ-PGA nanogels were fabricated by ultrasonication. The average size and surface charge of Dox-loaded disulfide cross-linked γ-PGA nanogels in aqueous solution were 136.3 ± 37.6 nm and -32.5 ± 5.3 mV, respectively. The loading amount of Dox was approximately 38.7 ?g per mg of γ-PGA nanogel. The Dox-loaded disulfide cross-linked γ-PGA nanogels showed controlled drug release behavior in the presence of reducing agents, glutathione (GSH) (1- 10 mM). Through fluorescence microscopy and FACS, the cellular uptake of γ-PGA nanogels into breast cancer cells (MCF-7) was analyzed. The cytotoxic effect was evaluated using the MTT assay and was determined to be dependent on both the concentration and treatment time of γ-PGA nanogels. The bio-derived γ-PGA nanogels are expected to be a well-designed delivery carrier for controlled drug delivery applications.