Glyoxal-induced exacerbation of pruritus and dermatitis is associated with <i>staphylococcus aureus</i> colonization in the skin of a rat model of atopic dermatitis

Han, Rafael Taeho,Kim, Hye Young,Ryu, Hyun,Jang, Wooyoung,Cha, Seung Ha,Kim, Hyo Young,Lee, JaeHee,Back, Seung Keun,Kim, Hee Jin,Na, Heung Sik Elsevier 2018 Journal of dermatological science Vol.90 No.3

<P><B>Abstract</B></P> <P><B>Background</B></P> <P>Atopic dermatitis (AD) is a highly pruritic, chronic inflammatory skin disease associated with hyperreactivity to environmental triggers. Among those, outdoor air pollutants such as particulate matter (PM) have been reported to aggravate pre-existing AD. However, underlying mechanisms of air pollution-induced aggravation of AD have hardly been studied.</P> <P><B>Objective</B></P> <P>To investigate the molecular mechanisms by which glyoxal, a PM-forming organic compound, exacerbates the symptoms of AD induced by neonatal capsaicin treatment.</P> <P><B>Methods</B></P> <P>Naïve and AD rats had been exposed to either fresh air or vaporized glyoxal for 5 weeks (2 h/day and 5 days/week) since one week of age. Pruritus and dermatitis were measured every week. The skin and blood were collected and immunological traits such as Staphylococcus aureus skin colonization, production of antimicrobial peptides and immunoglobulin, and mRNA expression of inflammatory cytokines were analyzed.</P> <P><B>Results</B></P> <P>Exposure to glyoxal aggravated pruritus and dermatitis in AD rats, but did not induce any symptoms in naïve rats. Staphylococcus aureus skin colonization was increased in the skin of both naïve and AD rats. Expression of antimicrobial peptides such as LL-37 and β-defensin-2 was also increased by exposure to glyoxal in the skin of both naïve and AD rats. The mRNA expression of Th1-related cytokines was elevated on exposure to glyoxal. However, serum immunoglobulin production was not significantly changed by exposure to glyoxal.</P> <P><B>Conclusion</B></P> <P>In AD rats, exposure to glyoxal exacerbated pruritus and cutaneous inflammation, which was associated with increased colonization of <I>S. aureus</I> and subsequent immunological alterations in the skin.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Exposure to glyoxal aggravated the symptoms in AD rats, but did not induce AD in naïve rats. </LI> <LI> <I>S. aureus</I> skin colonization and subsequent expression of antimicrobial peptides were increased after exposure to glyoxal. </LI> <LI> Exposure to glyoxal elevated the production of Th1-related cytokines such as TNF-α and IFN-γ in the AD lesional skin. </LI> </UL> </P>

Tb3+ and Ce^(3+) Intercalated Laponite Powder: The Influence of Ce^(3+) Ions on Thermal Stability and Optical Properties of Tb^(3+) Intercalated Laponite

Han-Na Lee,Youhyuk Kim 대한화학회 2011 Bulletin of the Korean Chemical Society Vol.32 No.4

Laponite samples intercalated with Tb^(3+) or Tb^(3+),Ce^(3+) ions were prepared by exchange of Na^+ ions in interlayers with Tb^(3+) or Tb^(3+),Ce^(3+) ions. Strong green and weak blue emissions under vacuum ultraviolet (VUV) excitation (λ =158 nm) were observed due to the ^5D_4→^7F_J and ^5D_3→^7F_J emission lines, respectively. Tb^(3+) ions in an interlayer of laponite mainly existed in ion pairs or clusters, as evidenced by the concentration-dependent luminescence of the Tb^(3+) ions on the relative intensities of the ^5D_3→^7F_J and the ^5D_4→^7FJ emission lines, due to the action of a cross-relaxation process. The addition of Ce^(3+) ions increased the thermal stability of Tb^(3+)intercalated laponite up to 650 ^oC and quenched the ^5D_3→^7F_J emission lines, probably by promoting the formation of Tb^(3+) ion pairs at relatively low Tb^(3+) concentrations.

Tb³⁺ and Ce³⁺ Intercalated Laponite Powder: The Influence of Ce³⁺ Ions on Thermal Stability and Optical Properties of Tb³⁺ Intercalated Laponite

Lee, Han-Na,Kim, You-Hyuk Korean Chemical Society 2011 Bulletin of the Korean Chemical Society Vol.32 No.4

Laponite samples intercalated with $Tb^{3+}$ or $Tb^{3+},Ce^{3+}$ ions were prepared by exchange of Na+ ions in interlayers with $Tb^{3+}$ or $Ce^{3+}$ ions. Strong green and weak blue emissions under vacuum ultraviolet (VUV) excitation (${\lambda}$ = 158 nm) were observed due to the $^5D_4{\rightarrow}^7F_J$ and $^5D_3{\rightarrow}^7F_J$ emission lines, respectively. $Tb^{3+}$ ions in an interlayer of laponite mainly existed in ion pairs or clusters, as evidenced by the concentration-dependent luminescence of the $Tb^{3+}$ ions on the relative intensities of the $^5D_3{\rightarrow}^7F_J$ and the $^5D_4{\rightarrow}^7F_J$ emission lines, due to the action of a cross-relaxation process. The addition of $Ce^{3+}$ ions increased the thermal stability of $Tb^{3+}$ intercalated laponite up to $650^{\circ}C$ and quenched the $^5D_3{\rightarrow}^7F_J$ emission lines, probably by promoting the formation of $Tb^{3+}$ ion pairs at relatively low $Tb^{3+}$ concentrations.

청각장애학교 농학생의 한국어능력시험(TOPIK) 어휘ㆍ문법 결과 분석

이한나ㆍ최상배(Lee Han naㆍChoi Sang bae) 한국특수교육학회 2016 특수교육학연구 Vol.50 No.4

염분 스트레스에 노출된 옥수수(Zea mays) 뿌리의 굴중성 반응

한두열(Duyeol Han),이영나(Young Na Lee),김여재(Yeo Jae Kim),박웅준(Woong June Park) 한국생명과학회 2008 생명과학회지 Vol.18 No.8

염분 스트레스에 노출된 옥수수(Zea mays) 유식물의 원뿌리는 길이생장이 억제되는데, 본 연구에서는 위와 같은 조건에서 뿌리의 굴중성 반응을 조사하였다. 고농도의 NaCl과 KCl에 의해 스트레스를 받은 옥수수 원뿌리는 수평 상태에서 중력 자극을 받기 시작한 지 2시간 이후부터 대조구에 비하여 현저한 굴중성 반응을 나타내었다. 이러한 굴중성 촉진 현상은 Na?가 아니라 K?에 의한 것이었으며, Cl?도 굴중성반응 촉진에 기여하였다. 이로부터 이들 K?와 Cl? 이온들이 세포 팽창에 필요한 삼투 조절자로 기능한다는 결론을 얻었다. 또한 염분 스트레스에 의하여 뿌리의 길이 생장이 억제되더라도 중력장 내에서의 위치 결정은 억제되지 않고 오히려 촉진된다는 점도 확인하였다. We investigated gravitropic responses in the primary root of maize (Zea mays) seedlings which were exposed to salt stress. The maize roots salt-stressed with higher than 100 mM NaCl or KCl started to reveal enhanced gravitropic curvature after 2 hours form the gravi-stimulation. Such a promotion was not caused by sodium phosphate, but invoked by potassium phosphate, indicating the active component is K?. Because NaCl increased gravitropic curvature, despite that Na? did not played any role, we evaluated the role for Cl? by comparing the effects of MgCl₂ and MgSO₄. The enhancement of the curvature only with MgCl₂ revealed that Cl? played a role in the gravi-response, indicating the involvement of anion channels. These results suggest that both of K? and Cl? play roles in the regulation of osmosis that is required for cell expansion in gravitropism as well as in nyctinasty and stomatal opening.

위암과 식도암이 병발된 다발성 원발성 악성종양 1예

박사영,이나영,이효진,이선영,최진혁,이순남,심강섭,성순희,한운섭 梨花女子大學校 醫科大學 醫科學硏究所 1996 EMJ (Ewha medical journal) Vol.19 No.3

Multiple primary cancer means that more that two cancers occur independently in an individual. Recently, the incidence of multiple primary cancer has increased with lengthened survival, of cancer patients, development of new diagnostic technique and increased clinical evaluation. We report a patient who had adenocarcinoma of stomach combined with squamous cell carcinoma of esophagus simultaneously.

원위부 신세뇨관성 산증에서 산-염기 운반체의 결손

김혜영,한진석,이정상,김현리,김진,이중건,이서진,김근호,진호준,전은실,주권욱,나기영,정우경,오지은,엄재호,궁성수 대한신장학회 2000 Kidney Research and Clinical Practice Vol.19 No.5

The purpose of this study was to elucidate whether the molecular defect of acid-base transporters in renal tubules is related to the functional defect of urinary acidification in distal renal tubular acidosis(RTA). We performed NH₄Cl, furosemide, or bicarbonate loading test to evaluate renal acidification function, and immunohistochemistry using antibodies to H^+ -ATPase, Cl^-/HCO₃^- exchanger(band-3 protein), and Na^+/K^+ -ATPase in kidney tissue in 6 patients with RTA and renal cell carcinoma patients as normal controls. Kidney tissue was obtained either by percutaneous needle biopsy(RTA) or nephrectomy(NC). The results were as follows; 1) In all six RTA patients, proton secretory defect of distal acidification was shown by a failure to lower the urine pH after NHC1 loading or furosemide test or abnormally low urine-blood pCO₂ difference during bicarbonate loading. In two patients with RTA, proximal acidification defect was combined, which was demonstrated by increased fractional excretion of bicarbonate. 2) In mal control, intense H^+ -ATPase and band-3 protein staining was observed in collecting ducts. 3) In distal RTA patients, H6+ -ATPase and band-3 protein staining was not demonstrable or markedly decreased in the intercalated cells of distal nephron. 4) In two patients who had both proximal and distal RTA, H^+ -ATPase staining was markedly decreased in the brush border of proximal tubules as well as the distal nephron. In conclusion, the defect of acid-base transporters in renal tubule was related with the functional defect of urinary acidification in distal RTA.

한국해안으로부터 Purple, Non-Sulfur Photosynthetic Bacterium, Rhodobacter sp. EGH-24의 분리 및 특성

차미선,김기한,조순자,이나은,이정은,이재동,이상준,박재림 한국환경과학회 2003 한국환경과학회지 Vol.12 No.12

A species of facultative photo-organotrophic, purple, non-sulfur bacterium was isolated from the 47 point at west and south coast of Korea in September 2001. Separated 13 samples of changes with red color under 28~32 ℃, 3000 lux, anaerobe conditions for 7 days cultivated in basal medium. For pure isolation from 13 samples, we used agar-shake tube method (0.4 % agar) and separated 5 strains through 13-repetition test. EGH-24 and EGH-30 was identified as the same strain through the RAPD(Random Amplified Polymorphic DNA)-PCR of strain EGH-9, EGH-13, EGH-23, EGH-24, EGH-30. Four isolates cultivated in synthesis wastewater for wastewater biodegradation test. EGH-24 was selected with efficient wastwater treating strain. Based on the results obtained from morphology, nutrient requirements, major bacteriochlorophyll content, 16S-rDNA phylogenetic analysis, EGH-24 strain may be identified as a new strain of the genus Rhodobacter and named Rhodobacter sp. EGH-24.

횡문근융해증과 급성 신부전 및 범발성 혈관내 응고장애를 동반한 열사병 1예

최대은,박수진,권오경,이한규,이영모,이상주,나기량,이강욱,신영태 충남대학교 의학연구소 2003 충남의대잡지 Vol.30 No.2

Heat stroke is a life-threatening illness characterized by an elevated core body temperature that rises above 40% and central nervous system dysfunctions that results in delirium, convulsions, or coma. Heat stroke is subdivided into two forms, classic and exertional. Since strenuous exercise is one of the major exacerbating and precipitating factors, the incidence of exertional heat stroke is high among young adult, especially military personnel undergoing military training. Exertional heat stroke commonly causes rhabdomyolysis, lactic acidosis, acute renal failure, shock and pulmonary edema. We report a case of heat stroke with rhabdomyolysis, acute renal failure, disseminated intravascular coagulopathy(DIC) after strenuous exercise. A 18 year old man was admitted to the hospital because of loss of consciousness. He was a wrestler and he had tried to lose 6kg in weight by jogging and strenuous exercise on that summer day with hot temperature. The development of this illness was favoured by much clothes inappropriate for that environmental condition with a purpose to enhance sweating, and by restricted intake of water and food. On admission, the blood pressure was 80/50mmHg, and body temperature was 39.4℃ with stuporous mentality. Laboratory finding were as follows : in complete blood cell count, hemoglobin was 16.7g/dL and platelet count was 158,000/ mm³. In blood chemistry, AST 1061U/L, ALT 201U/L, BUN 30mg/dL, Cr 2.2mg/dL, LDH 941IU/L, CK 696IU/L were resulted. In arterial blood gas analysis, pH 7.39, pCO₂ 30mmHg pO₂ 86mmHg, HCO₃ 18.4mEq/L, O₂ saturation 96.7% were resulted. In urinalysis, specific gravity 1.025, protein 3+, erythrocyte 2+ were resulted. In brain imaging study, brain CT finding was normal. After appropriate treatments with rapid cooling, hydration and supportive care, he improved with alert mental status. On 2nd hospital day, in laboratory result, thrombocytopenia and coagulopathy were appeared and renal insufficiency was aggravated. So he was treated with platelet concentrate and fresh frosen plasma for DIC, and enough hydration for appropriate urine volume preservation. After all, On 22nd hospital day, he fully recovered and discharged. Nowadays, with followed for some duration, he is wellbeing with no complications.

단열회로를 이용한 레지스터 회로의 설계

이한승,나인호,문용,이찬호 崇實大學校 生産技術硏究所 2001 論文集 Vol.31 No.-

An 8 x 16-b adiabatic register file with one read port and three write port and a 4-bit counter are designed. A four-phase clock generator is also designed provide supply clocks for adiabatic circuits. All the word line and bit line charge on the capacitive interconnections is recovered to save energy. Adiabatic circuits are based on efficient charge recovery logic (ECRL) and are designed using 0.35㎛ CMOS technology. The functionality of adiabatic circuits are verified and the energy consumption of adiabatic circuits are obtained using SPICE simulation. The energy consumption is 6.67∼9.17 pJ/cycle from 10㎒ to 100㎒ operating frequencies. The proposed adiabatic circuits are useful as the building blocks of low power circuits.