http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Characterization of proteolysis in muscle tissues of sea cucumber Stichopus japonicus
Chen-Chen Zhao,Yang Yang,Hai-tao Wu,Zhi-Mo Zhu,Yue Tang,Cui-Ping Yu,Na Sun,Qiang Lv,Jia-Run Han,Ao-Ting Li,Jia-Nan Yan,Yue Cha 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.6
The proteolysis in muscle tissues of sea cucumber Stichopus japonicus (sjMTs) was characterized. The proteins from sjMTs were primarily myosin heavy chains (MHCs), paramyosin (Pm), and actin (Ac) having a molecular mass of approximately 200, 98, and 42 kDa, respectively. Based on SDS-PAGE analysis and quantification of trichloroacetic acid (TCA)-soluble peptides released, degradation of muscle proteins from sjMTs was favorable at pH 5 and 50°C. Proteolysis of MHCs was mostly inhibited by cysteine protease inhibitors, including trans-epoxysuccinyl-L-leucyl-amido (4- guanidino) butane (E-64) and antipain (AP). E-64 and AP completely inhibited the degradation of Pm and Ac, while iodoacetic acid showed a partially inhibitory effect. These results indicated that the proteolysis of sjMTs was mainly attributed to cysteine proteases. Avoidance of setting the tissues at 40–50oC and slightly acidic condition and inhibition of cysteine proteases are helpful for decreasing sea cucumber autolysis.
Shou-Kuan Tsai,Chen-Chih Chen,Han-Jia Lin,Han-You Lin,Ting-Tzu Chen,Lih-Chiann Wang 대한수의학회 2020 Journal of Veterinary Science Vol.21 No.2
The pandemic of avian influenza viruses (AIVs) in Asia has caused enormous economic loss in poultry industry and human health threat, especially clade 2.3.4.4 H5 and H7 subtypes in recent years. The endemic chicken H6 virus in Taiwan has also brought about human and dog infections. Since wild waterfowls is the major AIV reservoir, it is important to monitor the diversified subtypes in wildfowl flocks in early stage to prevent viral reassortment and transmission. To develop a more efficient and sensitive approach is a key issue in epidemic control. In this study, we integrate multiplex reverse transcription recombinase polymerase amplification (RT-RPA) and capillary electrophoresis (CE) for high-throughput detection and differentiation of AIVs in wild waterfowls in Taiwan. Four viral genes were detected simultaneously, including nucleoprotein (NP) gene of all AIVs, hemagglutinin (HA) gene of clade 2.3.4.4 H5, H6 and H7 subtypes. The detection limit of the developed detection system could achieve as low as one copy number for each of the four viral gene targets. Sixty wild waterfowl field samples were tested and all of the four gene signals were unambiguously identified within 6 h, including the initial sample processing and the final CE data analysis. The results indicated that multiplex RT-RPA combined with CE was an excellent alternative for instant simultaneous AIV detection and subtype differentiation. The high efficiency and sensitivity of the proposed method could greatly assist in wild bird monitoring and epidemic control of poultry.
Review on Kinematics Calibration Technology of Serial Robots
Chen-Gang,Li-Tong,Chu-Ming,Jia-Qing Xuan,Sun-Han Xu 한국정밀공학회 2014 International Journal of Precision Engineering and Vol. No.
Robot kinematics calibration is of great significance for improving robot absolute pose accuracy, which can be divided into modelbasedand non-parametric kinematics calibration. For model-based kinematics calibration, kinematics calibration modeling, posemeasurement of end-effector, kinematics parameters calibration and error compensation are systematically analyzed. And with theincreasing demand for autonomy, the autonomous kinematics calibration is introduced. On the other hand, research status of nonparametrickinematics calibration is elaborated, the advantages and disadvantage of which are discussed respectively. On the basis,comprehensive and organized description and summarization of the three kinds of calibrations are given. Overall, aiming at robotkinematics calibration, existing achievements are summarized, a typical calibration process is carried out and calibration precautionsare detailed, then calibration results of various methods are compared and analyzed. Finally, existing problems in kinematicscalibration are analyzed and development prospects are presented.
Jia-Fong Jhang,Yuan-Hong Jiang,Yung-Hsiang Hsu,Han-Chen Ho,Lori A Birder,Teng-Yi Lin,Hann-Chorng Kuo 대한배뇨장애요실금학회 2022 International Neurourology Journal Vol.26 No.S1
Purpose: To investigate urothelial cell proliferation, cytoskeleton, inflammation, and barrier function protein expressions in patients with interstitial cystitis/bladder pain syndrome (IC/BPS) after intravesical platelet-rich plasma (PRP) injections Methods: A total of 19 patients with IC/BPS underwent 4 monthly intravesical PRP injections. Bladder biopsies were taken at the first and fourth PRP treatment. The bladder specimens were analyzed using the Western blot and immunochemical staining for progenitor cell markers for sonic hedgehog (Shh), CD34, and cytoskeleton proteins cytokeratin 5 (CK5), CK14, CK20; barrier function markers for zonula occludens-1 (ZO-1), E-cadherin, and intercellular adhesive molecule-1, tryptase and transforming growth factor-β (TGF-β). Global response assessment (GRA) was used to evaluate treatment outcomes. Results: The mean age of patients was 55.6 years. After PRP injections, the functional bladder capacity and maximum flow rate increased, and the visual analogue scale (VAS) of pain, interstitial cystitis (IC) symptom index, IC problem index, O’Leary-Sant symptom score, and GRA improved in all patients. Urothelium Shh, CK5, ZO-1, E-cadherin, and TGF-β expressions increased significantly after repeated PRP injections. By subgrouping, according to PRP treatment outcomes, significant increases in Shh, E-cadherin, and ZO-1 expressions were noted only in patients with GRA ≥1 or improved VAS, but not in patients with GRA=0 and no improvement in VAS. Conclusions: The level of urothelial barrier function protein and cell proliferation protein expression in the patients with IC/ BPS was increased after repeat intravesical PRP injections. Intravesical repeat PRP injections may have potential to improve urothelial health and result in symptoms improvement in the patients with IC/BPS.
Centrifuge Model Tests and Numerical Simulations of the Landslide Evolution Process
Han-Dong Liu,Jia-Xing Chen,Wen-Xi Han,Ye Wu,Dong-Dong Li 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.6
Centrifuge model tests and numerical simulations were performed to study the landslide evolution process and failure mechanism. A TLJ-500 geotechnical centrifuge was used for the experiments and landslide deformation and stress was monitored using high-precision differential displacement sensors and earth pressure micro-sensors. Discrete element numerical simulations were performed using PFC2D based on the experimental results. The findings show that the landslide evolution process can be divided into three stages: 1) compaction and consolidation; 2) uniform deformation; and 3) accelerated deformation and failure. The numerical simulation results verify the distinct stage characteristics of the landslide evolution process. According to the migration of microscopic soil mass particles within the landslide, stage 3) can be further divided into a deformation development stage and instability and failure stage. The simulation displacement monitoring curves and displacement map show distinct deformation characteristics and displacement indicators from stages 2) to 3) and from the deformation development stage to the instability and failure stage. The experimental and numerical results reveal the landslide failure mechanism: the upper part of the landslide thrusts and slides; the middle part squeezes; the lower part collapses; and shear plane penetration leads to landslide failure.
Kun Chen,Miaomiao Han,Mengyao Tang,Yadong Xie,Yuting Lai,Xianting Hu,Jia Zhang,Jun Yang,Huabin Li 대한천식알레르기학회 2018 Allergy, Asthma & Immunology Research Vol.10 No.6
Purpose: Hrd1 has recently emerged as a critical regulator of B-cells in autoimmune diseases. However, its role in the pathogenesis of chronic rhinosinusitis with nasal polyps (CRSwNP) remains largely unexplored. This study aimed to examine Hrd1 expression and B-cell accumulation and their possible roles in CRSwNP. Methods: Quantitative real-time polymerase chain reaction, immunohistochemistry, enzyme-linked immunosorbent assay and Western blotting were used to assess gene and protein expression in nasal tissue extracts. Cells isolated from nasal tissues and peripheral blood mononuclear cells were characterized by flow cytometry. Local antibody production was measured in tissue extracts with a Bio-Plex assay. Additionally, changes in Hrd1 expression in response to specific inflammatory stimuli were measured in cultured dispersed polyp cells. Results: Nasal polyps (NPs) from patients with eosinophilic CRSwNP (ECRS) had increased levels of Hrd1, B-cells and plasma cells compared with NPs from patients with non-eosinophilic CRSwNP (non-ECRS) or other control subjects (P < 0.05). The average Hrd1 levels in B-cells in NPs from ECRS patients were significantly higher than those from non-ECRS patients and control subjects (P < 0.05). NPs also contained significantly increased levels of several antibody isotypes compared with normal controls (P < 0.05). Interestingly, Hrd1 expression in cultured polyp cells from ECRS patients, but not non-ECRS patients, was significantly increased by interleukin-1β, lipopolysaccharide and Poly(I:C) stimulation, and inhibited by dexamethasone treatment (P < 0.05). Conclusions: Differential Hrd1 expression and B-cell accumulation between the ECRS and non-ECRS subsets suggests that they can exhibit distinct pathogenic mechanisms and play important roles in NP.
Boundary-Aware Dual Attention Guided Liver Segment Segmentation Model
( Xibin Jia ),( Chen Qian ),( Zhenghan Yang ),( Hui Xu ),( Xianjun Han ),( Hao Ren ),( Xinru Wu ),( Boyang Ma ),( Dawei Yang ),( Hong Min ) 한국인터넷정보학회 2022 KSII Transactions on Internet and Information Syst Vol.16 No.1
Accurate liver segment segmentation based on radiological images is indispensable for the preoperative analysis of liver tumor resection surgery. However, most of the existing segmentation methods are not feasible to be used directly for this task due to the challenge of exact edge prediction with some tiny and slender vessels as its clinical segmentation criterion. To address this problem, we propose a novel deep learning based segmentation model, called Boundary-Aware Dual Attention Liver Segment Segmentation Model (BADA). This model can improve the segmentation accuracy of liver segments with enhancing the edges including the vessels serving as segment boundaries. In our model, the dual gated attention is proposed, which composes of a spatial attention module and a semantic attention module. The spatial attention module enhances the weights of key edge regions by concerning about the salient intensity changes, while the semantic attention amplifies the contribution of filters that can extract more discriminative feature information by weighting the significant convolution channels. Simultaneously, we build a dataset of liver segments including 59 clinic cases with dynamically contrast enhanced MRI(Magnetic Resonance Imaging) of portal vein stage, which annotated by several professional radiologists. Comparing with several state-of-the-art methods and baseline segmentation methods, we achieve the best results on this clinic liver segment segmentation dataset, where Mean Dice, Mean Sensitivity and Mean Positive Predicted Value reach 89.01%, 87.71% and 90.67%, respectively.