사람 혈장 중 부메타니드의 HPLC 분석법 검증 및 단일 용량 투여에 의한 약물동태 연구

박혜영,곽혜선,전인구 한국약제학회 2005 Journal of Pharmaceutical Investigation Vol.35 No.1

A high-performance liquid chromatographic method was employed for the determination of bumetanide in human plasma. After addition of internal standard (IS, naproxen) and acidification of the plasma with 1 M hydrochloric acid, the drug and IS were extracted into dichloromethane. The organic phase was back-extracted into 1 M sodium bicarbonate solution and 50 μl of the aqueous phase was injected onto a reversed-phase C18 column with a mobile phase consisting of methanol : water : glacial acetic acid = 65 : 35 : 1. The samples were detected utilizing a fluorescence detector (excitation wavelength 235 nm, emission wavelength 405 nm). The method was specific and validated with a lower limit of 5 ng/mL. Intra- and inter-day precision and accuracy were acceptable for all quality control samples including the lower limit of quantification. The applicability of the method was demonstrated by analysis of plasma after oral administration of a single 2 mg dose to 24 healthy subjects. From the plasma bumetanide concentration vs. time curves, the mean AUC was 246.5 ± 73.8 ng hr/mL and C_(max) of 132.1 ± 40.9 ng/mL reached 1.2 hr after administration. The mean biological half-life of bumetanide was 1.1 ± 0.2 hr. Based on the results, this simple and validated assay method could readily be used in any pharmacokinetic or bioequivalence studies using humans.

알레르기 환자에서 특이알레르겐 항체 양성율과 관련요인

이선화,남해선,이성수,안현철,황규윤,박준수,,박춘식 순천향의학연구소 2002 Journal of Soonchunhyang Medical Science Vol.8 No.2

Recently, allergic diseases are very prevalent in Korea, resulting in physical and economic losses during the life. It is well-known that the principle therapy is identification, eradication, and desensitization of allergens. There are many allergic diagnostic tools for identification of specific-allergens. However, none of them is satisfied with its efficiency due to lack of validity. Recently MAST-CLA has been widely used in the allergic clinical fields because of its simplicity and simultaneous identification of allergens. This study was designed to determine the positivity of allergen-specific antibodies using MAST-CLA in patients with allergic diseases, and evaluate the efficacy for allergen detection, compared to results of skin prick test. During June 2000 - September 2001, 325 study subjects visited Soonchunhyang University Chunan Hospital for the evaluation of allergic problems. Among them, 289 subjects were diagnosed with allergic disease and included for the study. Thirty four subjects visited children's allergic unit were evaluated specific allergens using a skin prick test at the same time. Our result as follows; 1. The positive rates of allergen-specific antibodies and total IgE were 51.2% and 73.4%, retrospectively. 2. There was significant difference in positive rate in age group, showing highest in teen ages(73.6%), but insignificant difference in sex in allergen-specific antibodies. 3. The positive rate was 60.4% in allergic rhinitis, 51.3% in allergic dermatitis, 46.9% in bronchial asthma, and 39.1% in urticaria in order(P=0.073). 4. Significant difference in month or season was observed, with highest rate in April(78.1%) and May(72.1%). The highest season was in spring(62.7%), and then summer(52.8%), fall(50.3%), and winter(41.4%) in order (P=0.04). 5. The three highest allergen-specific antibodies were D. pteronyssinus(39.3%), D. farinae(38.3%), and house dust(24.3%) in Korean inhalant pannel, However, in food pannel types of allergen-specific antibody were different as D. farinae(18.5%), Alternaria(18.5%), house dust(17.3%). 6. There were significant agreements between MAST-CLA and Skin prick test (D. farianae Kappa=0.636;D. pteronyssinus Kappa=0.78, both P<0.001) In conclusion, MAST-CLA could detect allergen-specific antibodies in patients with allergic diseases as a simple and valid tool of screening of allergy. Especially, it could be a useful measure of multiple allergens simultaneously in children However, more detailed allergens and valid assay for unmeasured allergens should be identified for Korean-specific allergens.

都市主婦의 結婚 適應度에 關한 硏究

金惠貞,李蓮淑,任春淑,鄭明姬,曺鏞旬,黃智暎 효성여자대학교 가정대학 학도호국단 1984 家政大論集 Vol.3 No.-

We measured general marital adjustment of the housewives who ilve in Daegu. We analysed marital affectional expression, cohesion, consensus and satisfaction and also analysed marital adjustment according to the level of education or length or marriage. The results are as follows; 1. The level of general marital adjustment of the urban housewives was medium. 2. According to factor analysis, the level of marital affectional expression was medium, the level of marital satisfaction and consensus was high, and the level of cohesion was low. 3. The more educated, the higher the degree of marital adjustment in general. 4. The marital adjustment was lowest in housewives whose length of marriage was 9-11 years. The second lowest were 3-5 years and 21-23 years. The highest was 15-17 years.

국내에서 유행하는 HIV의 전파 경로에 따른 Subtype 분포

이주실,남정구,김성순,강춘,최병선,김옥진,박미선,성봉모,서순덕,전수경,변승옥,신영오,조해월 대한감염학회 2001 감염 Vol.33 No.5

Background : Previous data have been reported that subtype B is prevalent in South Korea, but neither the extent nor the proportion of subtypes could be evaluated. This study was designed to analyze the distribution of HIV-1 subtypes, temporal instructions and transmission dynamics between epidemiological groups. Methods : 1,280 Koreans had been diagnosed as HIV seropositive during the period 1985 to 2000. Among them, 134 individuals were selected for this molecular epidemiological study. 134 DNAs were isolated from uncultured or cultured peripheral blood mononuclear cells. V3-V5 (0.7 kb) fragment of HIV-1 env gene was amplified by nested polymerase chain reaction and was sequenced. Results : HIV-1 isolates from thirty-seven homosexuals were all subtype B (100%). On the other hand, 66 isolates from 94 heterosexuals were subtype B (70%) and 28 were non B subtypes (30% : 13 A, 4 C, 2 D, 8 E , 1 G). Only subtype B strains were isolated from 73 males who were infected with HIV inside Korea while 16 B and 20 non B subtype strains were isolated from 36 males who were HIV infected outside of Korea. However, B and non B strains were isolated half and half from females who were infected inside Korea except one. Conclusion : The HIV-1 subtype B strains are prevalent in Korea from the early HIV infection until present in both homo and heterosexuals. Non B strains have been transmitted from men who were infected outside Korea to their spouses and casual partners. So, we need further study to monitor subtype B and non B HIV transmission in epidemiological groups of Korea, (Korean J Infect Dis 33:311∼318, 2001)

길찾기 좋은 서울 (Legible Seoul)을 위한 전략 -지하철역 길찾기 시스템을 중심으로

( Hae Jin Yeo ),( Dong Hoon Lee ),( Chang Yi ),( Hae Sun Chun ),( Sun Young Seo ) 서울시정개발연구원 2011 연구보고서 Vol.2011 No.1

This study aims to explore problems of the way-finding system of Seoul subway station. In case of Seoul, there has been many problems of losing a way, looking around, going on a bootless errand, going back, asking and so forth for subway users whether they are foreigners or not. Specifically focused on four densely-used subway stations which are Seoul Station, City Hall Station, Express Bus Terminal Station, Myeong-dong Station, the research arguments are developed through site visit, investigation and analysis, discussion meeting and interviews. Investigating the real condition of signs in four subway stations, 3 types of problems are determined. The most serious problem is that the signs is not working as a way-finding system at the level of transfer guide, way-out and upper space guide, and circulation decision point guide. These problems are mostly driven because of failing to properly locate the sign and to provide the necessary level of information detail. The reason of those problems is examined from the point of planning process, production structure, and finally organization. In overall, the production of subway signs pre-empt sign system planning as well as subway sign manual do subway design guideline. Also the subway sign guideline and manual have not been developed based on behavioral and cognitive study, so it does not have any exact measuring standard and human scale to locate the signs. Policy implication is to suggest a supplimentation of subway sign guideline and manual based on user`s behavioral and cognitive characteristics, integrating interagency for better quality control of way-finding system, and several demonstration projects to improve sign system and remodeling projects.

Poster Session 2 : Cellular signal transduction and oncogenes ; The self-phosphorylation on 73-threonine residue of Akt protein kinase reduces its binding to phosphotidylinositol-3,4- diphosphate

( Sun Yong Shin ),( Jae Sun Chun ),( Ji Hae Lee ),( Tae Gun Kwon ),( Sang Sun Kang ) 한국생화학분자생물학회 (구 한국생화학회) 2000 추계학술대회집 Vol.2000 No.-

길찾기 좋은 서울(Legible Seoul)을 위한 전략 -지하철역 길찾기 시스템을 중심으로

여혜진 ( Hae Jin Yeo ),이동훈 ( Dong Hoon Lee ),이창 ( Chang Yi Hae ),전혜선 ( Hae Sun Chun ),서선영 ( Sun Young Seo ) 서울시정개발연구원 2011 연구보고서 Vol.2011 No.22

Protective Effect of an Herbal Preparation (HemoHIM) on 5-Fluorouracil-Induced Intestinal Injury in Mice

Hae June Lee,Joong Sun Kim,Changjong Moon,Jong Choon Kim,Chun Sik Bae,Sung Soo Kang,Uhee Jung,Hae Ran Park,Sung Kee Jo,Sung Ho Kim 한국실험동물학회 2009 Laboratory Animal Research Vol.25 No.1

The morphological changes of small intestine and protective properties of an herbal preparation (HemoHIM) in intestinal damages were examined by evaluating its effects on jejunal crypt survival, apoptosis and other histological assessment in 5-fluorouracil (5-FU)-treated mice. The female ICR mice were treated with 5-FU for the examination of jejunal crypt survival and any morphological changes (200 ㎎/㎏ of body weight, i.p.), and for the detection of apoptosis (40 ㎎/㎏ of body weight, i.p.). HemoHIM was administered intraperitoneally at a dosage of 50 ㎎/㎏ of body weight at 36 and 12 hours preirradiation and 30 minutes post-irradiation, or orally at a dosage of 250 ㎎/㎏ of body weight/day for 7 or 11 days before autopsy. Compared to normal controls, 5-FU increased the incidence of apoptosis by 20-fold. 5-FU decreased villus height in the jejunum (44.6%) with crypt depths increased by 18.6% in this gut region. In comparison with normal controls, the values of basal lamina length of 10 enterocytes in the jejunum were significantly higher in 5-FU treated mice (55.5%). These effects were less profound in HemoHIM treated mice in which apoptosis was decreased 11.4% (p.o.) and 27.4% (i.p.), with villus height increased by 37% (p.o.) and 28% (i.p.), crypt depth by 13.4% (p.o.) and 19.0% (i.p.) and accompanied by decreases in basal lamina length of 23.5% (p.o.) and 31.9% (i.p.), compared to 5-FU control group. These results suggested that HemoHIM may be therapeutically useful to reduce intestinal injury following 5-FU treatment.

Significant Change of Stress-related Proteins and Gene Profile in the Liver of Long-term Ground Transported Animals

Sun Bo Shim,Se Hyun Lee,Dae Youn Hwang,Chuel Kyu Kim,Bung Guk Kim,Yong Kyu Kim,Seung Wan Jee,Su Hae Lee,Ji Soon Sin,Chang Jun Bae,Byoung Chun Lee,Mi Kyong Jang,Min Sun Kim,Su Youn Yim,Jung Sik Cho,Kab 한국실험동물학회 2007 Laboratory Animal Research Vol.23 No.1

The transportation stress cause rise in the levels of many hormones and proteins to maintain homeostasis in the laboratory animals. In order to study whether the stress induced by long-term ground transport could contribute to change in the stress-related protein and profile of stress-regulated gene expression, these factors were detected in the animals between before and after the ground transportation. Herein, it is concluded that; ⅰ) the transported mice significantly exhibited the increase of corticosterone concentration in serum. ⅱ) the heat shock protein 70 (HSP70) in the liver were significantly decreased in the transported animals. ⅲ) 372 and 272 genes were up- and down-regulated, respectively, by stress in the long-term ground transported mice compare to the controls. ⅳ) genes encoding for immunity, signal transduction response and response to protein metabolism were significantly over-represented in the up-regulated group. ⅴ) among the down-regulated transcripts, those encoding for protein metabolism were dramatically over-represented, followed by those related to lipid metabolism and development process. These results suggested that the stress induced by ground transportation could significantly changed the level of stress-indicator hormone, proteins and gene profile in the mice. Therefor, the animal transported a long distance should be had the adaptation period to recover the normal physical state.

The Effect of Dongchongxiacao (Paecilomyces japonica) on Ultraviolet B-Induced Skin Damages in Mouse

Hae-June Lee,Se-Ra Kim,Joong-Sun Kim,Sun-A Kee,Jong-Choon Kim,Chun-Sik Bae,Jong-Sik Jang,Sung-Kee Jo,Sung-Ho Kim 한국실험동물학회 2006 Laboratory Animal Research Vol.22 No.3

The effect of the fruiting bodies of Paecilomyces japonica (PJ), grown on silk-worm larvae on the changes of ultraviolet (UV) light B radiation-induced apoptotic sunburn cell (SBC) and epidermal ATPase-positive dendritic cell (DC) in SKH1-hr or ICR mouse were investigated. The mice were treated with UVB (200 mJ/㎠) and were sacrificed 24 hours later. PJ (50 ㎎/㎏ of body weight) or vehicle (saline) was given i.p. at 36 and 12 hours before irradiation, and 30 minutes after irradiation or PJ cream (0.2%) or cream base (vehicle) was topically treated at 24 hours and 15 minutes before irradiation, and immediately after irradiation. The skin of SKH1-hr mouse prepared from the back of untreated mice exhibited about 0.3 SBC/㎝ length of epidermis, and 24 hours after UV irradiation, the applied areas show an increased number of SBCs. But the frequency of UVB-induced SBC formation was reduced by intraperitoneal injection of PJ extract (p < 0.01). The numbers of DC in normal ICR mouse were 628.00± 51.56 or 663.20± 62.58 per ㎟ of ear epidermis. By 1 day after UVB treatment, the number of ATPase-positive cells/mrn? were decreased by 39.0% or 27.1% in i.p. or topical application group with vehicle. Treatment of PJ was associated with reductions of 30.8% in i.p, group (p < 0.05) and 8.4% in topical application group in the number of ATPase positive cells compared with the irradiation control group. The results presented herein that PJ administration could reduce the extent of skin damages produced by UVB.