Photobacterium aplysiae sp. nov., a lipolytic marine bacterium isolated from eggs of the sea hare Aplysia kurodai

Seo, Hae Jeom,Bae, Seung Seob,Yang, Sung Hyun,Lee, Jung-Hyun,Kim, Sang-Jin Microbiology Society 2005 International journal of systematic and evolutiona Vol.55 No.6

<P>A bacterium, named GMD509<SUP>T</SUP>, showing lipolytic activity was isolated from the eggs of the sea hare <I>Aplysia kurodai</I> collected at Mogiyeo (depth, 12 m), an uninhabited small island in the South Sea of Korea. The strain is Gram-negative, motile, facultatively anaerobic, mesophilic and weakly halophilic. Optimal growth of strain GMD509<SUP>T</SUP> occurs in the presence of 3·0 % (w/v) NaCl and at pH 8 and 25 °C. The whole-cell fatty acid profile of the isolate includes C16 : 1, C16 : 0 and C18 : 1 as major fatty acids and its DNA G+C content is 45 mol%. Phylogenetic analyses of 16S rRNA gene sequences place this bacterium in the <I>γ</I>-<I>Proteobacteria</I>, within the genus <I>Photobacterium</I>. The 16S rRNA gene sequence of strain GMD509<SUP>T</SUP> is most similar to those of <I>Photobacterium frigidiphilum</I> (97·8 %), <I>Photobacterium profundum</I> (97·5 %) and <I>Photobacterium indicum</I> (97·4 %). DNA-DNA relatedness levels between the isolate and its closest known phylogenetic relatives, <I>P. frigidiphilum</I> and <I>P. indicum</I>, are 25·3 and 13·7 %, respectively. Strain GMD509<SUP>T</SUP> therefore represents a novel species, for which the name <I>Photobacterium aplysiae</I> sp. nov. is proposed, with the type strain GMD509<SUP>T</SUP> (=KCTC 12383<SUP>T</SUP>=JCM 12948<SUP>T</SUP>).</P>

Photobacterium frigidiphilum sp. nov., a psychrophilic, lipolytic bacterium isolated from deep-sea sediments of Edison Seamount

Seo, Hae Jeom,Bae, Seung Seob,Lee, Jung-Hyun,Kim, Sang-Jin Microbiology Society 2005 International journal of systematic and evolutiona Vol.55 No.4

<P>A Gram-negative, motile, rod-shaped, psychrophilic and weakly halophilic bacterial strain, SL13<SUP>T</SUP>, was isolated from deep-sea sediments (1450 m depth) of Edison Seamount in the western Pacific Ocean. Optimal growth of SL13<SUP>T</SUP> requires the presence of 1·5 % (w/v) NaCl, a pH of 6·0 and a temperature of 14 °C. The whole-cell fatty acid profile of the isolate includes C16 : 1 and C16 : 0 as major fatty acids and contains C20 : 5<I>ω</I>3. This is consistent with corresponding data for <I>Photobacterium profundum</I>. The DNA G+C content of strain SL13<SUP>T</SUP> is 43·8 mol%. Phylogenetic analyses of 16S rRNA gene sequences place this bacterium in the ‘<I>Gammaproteobacteria</I>’, within the genus <I>Photobacterium</I>. Sequence similarity analysis indicates that the closest relatives of strain SL13<SUP>T</SUP> are <I>Photobacterium indicum</I> (99·3 %), <I>P. profundum</I> (98·5 %) and <I>Photobacterium lipolyticum</I> (98·2 %). The DNA-DNA hybridization levels between the isolate and its closest known phylogenetic relatives, <I>P. indicum</I>, <I>P. profundum</I> and <I>P. lipolyticum</I>, are 27·1, 52·4 and 20·2 %, respectively. Thus strain SL13<SUP>T</SUP> represents a novel species of the genus <I>Photobacterium</I>, for which the name <I>Photobacterium frigidiphilum</I> sp. nov. is proposed. The type strain is SL13<SUP>T</SUP> (=KCTC 12384<SUP>T</SUP>=JCM 12947<SUP>T</SUP>).</P>

멸치젓 숙성중 지질의 산화와 지방산 조성의 변화

서해점(Hae Jeom Seo),정보영(Bo Young Jeong),남택정(Taek Jeong Nam),변재형(Jae Hyeung Pyeun) 한국수산과학회 1998 한국수산과학회지 Vol.31 No.2

Anchovy, Engraulis japonica, was fermented with 10% and 20% salt at 10℃(10% SA) and 20℃(20% SA), respectively, and the changes in lipid content, fatty acid composition and lipid oxidation was observed for 105 days. Peroxide value(POV) in 10% SA reached to the maximum(46.4meq/㎏) on 30 days of fermentation, while in 20% SA, it reached the maximum (54.7meq/㎏) on 45 days of fermentation, and then decreased in both samples during fermentation. Thiobarbituric acid values revealed a similar tendency with the change of POV during the fermentation. These results indicated that lipid of the anchovy was oxidized faster in lower salt than high salt in the early stage. Total lipid(TL) content during the fermentation for 105 days decreased approximately 16% in 10% SA and approximately 33% in 20% SA. Phospholipid(PL) content also decreased in both samples and the content of neutral lipid(NL) was unchanged in 10% SA, while it decreased in the same amount as PL in 20% SA. The prominent fatty acids in TL of the anchovy sample were 14 : 0, 16 : 0, 16 : 1n-7, 18 : 1n-9, 20 : 5n-3 and 22 : 6n-3. After fermentation for 105 days, approximately 87% and 67% of the prominent fatty acids remained in 10% SA and 20% SA, respectively, but the kind of the fatty acids was unchanged.

멸치젓 숙성중 지질의 산화와 지방산 조성의 변화

서해점(Hae Jeom Seo),정보영(Bo Young Jeong),남택정(Taek Jeong Nam),변재형(Jae Hyeung Pyeun) 경상대학교 해양산업연구소 1998 해양산업연구소보 Vol.10 No.-

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오이 발효음료가 만성적으로 에탄올을 급여한 흰쥐의 에탄올 대사와 항산화방어계에 미치는 영향

이해인(Hae-In Lee),서권일(Kwon-Il Seo),이진(Jin Lee),이점숙(Jeom-Sook Lee),홍성민(Sung-Min Hong),이주혜(Ju-Hye Lee),김명주(Myung-Joo Kim),이미경(Mi-Kyung Lee) 한국식품영양과학회 2011 한국식품영양과학회지 Vol.40 No.8

본 연구는 다량으로 폐기되는 오이를 이용하기 위하여 개발한 오이 발효원액을 주원료로 제조한 숙취해소 음료의 간보호 효능을 검증하기 위하여 만성적으로 에탄올을 섭취시킨 흰쥐에서 에탄올 대사, 항산화 방어계, 간독성 관련지표 및 지질함량 변화를 살펴보았다. 실험동물은 4 주령의 수컷 SD계 흰쥐 24마리를 1주간 고형식이로 적응시킨 후 난괴법에 의하여 에탄올대조군(Control) 및 에탄올 섭취 흰쥐에게 헛개열매 추출물을 주원료로 하여 개발한 숙취해소 물질인 SKM 급여군(SKM) 또는 SKM을 함유한 오이 발효음료 급여군(CF+SKM)으로 나누었다. SKM과 CF+SKM은 사람의 하루 섭취량을 기준으로 체중 ㎏당 7 ㎖씩 매일 일정시각에 경구투여 하였다. SKM과 CF+SKM은 체중과 식이섭취에는 영향을 미치지 않았으며, CF+SKM군의 신장무게가 대조군보다 낮았다. 혈장 중 에탄올 함량은 대조군에 비하여 CF+SKM군에서 유의적으로(p<0.05) 낮았으며, SKM군은 낮은 경향을 보였다. 혈장 중의 아세트알데히드 함량은 대조군에 비하여 SKM과 CF+SKM군 모두 각각 40.6%와 48.4% 유의적인(p<0.05) 개선 효과를 보였다. 간조직 중의 ADH 활성은 실험군간 유의적인 변화가 없었으나 CYP2E1 활성은 SKM과 CF+SKM 모두 대조군에 비하여 유의적으로 (p<0.05) 낮았다. 간조직의 CYP2E1 활성은 혈장 중의 아세트알데히드 함량과 양의 상관관계(r=0.566, p<0.01)였다. 간조직의 ALDH 활성은 SKM과 CF+SKM 모두 대조군에 비하여 유의적으로(p<0.05) 높았으며 혈장의 아세트알데히드 농도와 유의적 음의 상관관계(r=-0.564, p<0.01)를 보였다. SKM군과 CF+SKM군의 간조직내 SOD와 CAT 활성과 GSH 함량이 대조군에 비하여 유의적으로 높았다. 반면, SKM과 CF+SKM은 간조직 중의 지질과산화물 생성을 대조군에 비하여 각각 유의적으로 낮추었다. SKM과 CF+SKM 급여 시 에탄올대조군에 비하여 각각 AST 활성은 29%와 44% 낮았으며, ALT 활성은 42%와 34% 낮았다. 혈장의 총 콜레스테롤과 간조직의 콜레스테롤 함량은 대조군에 비하여 SKM과 CF+SKM군에서 유의적으로(p<0.05) 낮았으며 특히, CF+SKM의 간조직내 중성지질 함량은 대조군에 비하여 유의적으로(p<0.05) 낮았다. SKM군과 CF+SKM군의 간조직 중 지방축적이 대조군에 비하여 감소되었다. 이와 같이 SKM과 CF+SKM은 간조직의 CYP2E1 활성을 억제하고 ALDH 활성과 항산화 방어계를 향상시킴으로써 에탄올로 인한 간독성을 보호할 수 있을 것으로 사료된다. Cucumber fermentation has been used as a means of preservation. This study was performed to investigate the effects of fermented cucumber beverage (CF) containing beneficial materials for an ethanol hangover based on Hovenia dulcis (SKM) on ethanol-induced hepatotoxicity. Male Sprague-Dawley rats were randomly divided into three groups: ethanol control, ethanol plus SKM, and ethanol plus CF+SKM. SKM or CF+SKM was orally administered at a dose of 7 ㎖/㎏ body weight once per day for 5 weeks. Control rats were given an equal amount of water. CF+SKM significantly lowered plasma ethanol levels, whereas SKM tended to decrease the levels compared to the control. Both SKM and CF+SKM significantly lowered the plasma acetaldehyde levels and serum transaminase activities compared to those in the control. SKM and CF+SKM did not affect hepatic alcohol dehydrogenase activity; however, it significantly inhibited cytochrome P450 2E1 (CYP2E1) activity. Hepatic aldehyde dehydrogenase (ALDH) activity was significantly higher in the SKM and CF+SKM groups than that in the control group. Plasma acetaldehyde concentration was significantly correlated with hepatic CYP2E1 (r=0.566, p<0.01) activity and ALDH (r=-0.564, p<0.01) activity. Hepatic superoxide dismutase and catalase activities as well as glutathione content increased with the SKM and CF+SKM administration, whereas lipid peroxide content decreased significantly. Furthermore, SKM and CF+SKM lowered plasma and hepatic lipid content and lipid droplets compared to those in the control group. These results indicate that SKM and CF+SKM exhibit hepatoprotective properties partly by inhibiting CYP2E1 activity, enhancing ALDH activity and stimulating the antioxidant defense systems in ethanol-treated rats.

Effect of Deoxygenizer on the Suppression of Lipid Deterioration of Boiled and Dried-Anchovy Engraulis japonica: Changes in Lipid Class Compositions

( Bo Young Jeong ),( Hae Jeom Seo ),( Soo Kyung Moon ),( Jae Hyeung Pyeun ) 한국수산학회 1995 한국수산과학회지 Vol.28 No.6

Anchovy Engraulis japonica boiled and dried was stored at 20℃ for 5 months after that treatment of sodium-erythorbate (Na-ery) or deoxygenizer (Deoxy). During storage, peroxide value (POV), thiobarbituric acid (TBA) value, lipid content, and lipid class compositions were determined to evaluate the quality of the samples. POV was decreased rapidly for the first 3 months storage and its decrease was Deoxy group>Control group>Na-ery group in that order. TBA values increased for the first 4 months and then decreased rapidly, and it`s increase was the highest in Control group, followed by Na-ery and Deoxy group. Total lipid contents in all samples declined during storage. Especially, phospholipid decreased mainly in Na-ery and Deoxy group, while neutral lipid mainly in Control group. Triglyceride (TG), phosphatidylethanolamine (PE), and phosphatidylcholine (PC) decreased, while free fatty acid (FFA) and lyso-PC (LPC) increased during storage. The decrease of TG was the highest in Control group and that of PE and PC was higher in Na-ery group than in other sample. The decrease of PE in all samples (except Deoxy group) was higher than that of PC. The increase of FFA and LPC were higher in Control and Na-ery group than in Deoxy group. These results indicated that the lipid deterioration of the boiled and dried-anchovy was effectively suppressed by the enclosed deoxygenizer during storage at 20℃.

Screening, cloning and characterization of ofloxacin ester-hydrolyzing esterase from the yeast Yarrowia lipolytica 180

Jun-Tae Kim(김준태),Hae Jeom Seo(서해점),Byeong Chul Jeong(정병철),Jung Hyun Lee(이정현),Sang-Jin Kim(김상진) 한국생물공학회 2004 한국생물공학회 학술대회 Vol.2004 No.10

Effect of Deoxygenizer on the Suppression of Lipid Deterioration of Boiled and Dried-Anchovy Engraulis japonica: Changes in n-3 Polyunsaturated Fatty Acids

( Bo Young Jeong ),( Hae Jeom Seo ),( Soo Kyung Moon ),( Jae Hyeung Pyeun ) 한국수산학회 1995 한국수산과학회지 Vol.28 No.6

The effects of deoxygenizer (Deoxy) and sodium-erythorbate (Na-ery) on the changes in fatty acid compositions were investigated to prevent the loss of n-3 polyunsaturated fatty acids in lipid of bolied and dried-anchovy during storage. After storage for 5 months, docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) of total lipid (TL) in Deoxy group decreased only 3.0% and 0.5%, respectively, compared to those of before storage. However, those in Control group decreased 9.5% and 2.3%, respectively. In the case of Na-ery group, the percentages of DHA and EPA decreased were lower than those of Control. Most of DHA and EPA in TL was lost in the early stage of storage. Total DHA remained in phospholipid (PL), triglyceride (TG), and free fatty acid (FFA) fractions after storage for 5 months were 98%, 66% and 62% in Deoxy, Na-ery and Control group, respectively, and total EPA remained was slightly high level compared with those of DHA. The loss of DHA was more in PL than in TG. Consequently, deoxygenizer was recognized as a good material to prevent the loss of DHA and EPA of the boiled and dried-anchovy during storage.

Synthesis and Biological Activities of Myomodulin E and its Analogs

Hye-Jin Go(고혜진),Jung-Kil Seo(서정길),Hae Jeom Seo(서혜점),Min Jeong Lee(이민정),Tae Hyun Park(박태현),Gun Do Kim(김군도),Nam Gyu Park(박남규) 한국생명과학회 2012 생명과학회지 Vol.22 No.4

군소인 Aplysia kurodai의 중추신경절로부터 발견된 myomodulin A (MMA, PMSMLRLamide)와 myomodulin E (MME, GLQMLRLamide)는 Mytilus edulis의 anterior byssus retractor muscle (ABRM)을 활성측정시스템으로 사용하여 정제되었다. . 정제된 MMA와 MME는 연체동물에서 발견된 myomodulin 계열의 펩타이드와 동일한 일차구조를 지닌다. MME의 구조와 활성간의 상관관계를 알아보기 위해서 MME, 유도체 및 다른 신경성 펩타이드들을 합성하였다. MME의 유도체인 Des[Gly¹]-MME, Des[Gly¹,Leu²]-MME 및 Des[Gly¹, Leu²,Gln³]-MME의 일차구조는 각각 LQMLRLamide, QMLRLamide 및 MLRLamide이다. 합성 물질들을 사용하여 ABRM에 대한 phasic -contraction을 측정하였다. MME는 1×10<SUP>-9</SUP> M 또는 더 높은 농도에서 ABRM의 phasic contraction을 저해하였다. 또한 MME는 1×10<SUP>-8</SUP> M에서 catch-tension에 대해 이완활성을 나타내었다. 합성 펩타이드들을 사용하여 Africa giant snail, Achatina fulica의 소낭과 penial retractor muscle에 대해서도 활성을 측정하였다. MME와 유도체들은 소낭에 대해서는 수축반응을 보였지만, penial retractor muscle에 대해서는 이완 활성을 나타내었다. 이러한 결과들은 MME와 그 유도체들은 연체동물의 다양한 조직에 대해 조절 효과를 가지고 있다는 것을 의미한다. 본 연구는 생체 내에서 발생하는 신경 및 circuit의 변화를 조절하는 작용 연구에 대한 기본적인 자료가 될 것이다. Previous work has characterized myomodulin A (MMA, PMSMLRLamide) and myomodulin E (MME, GLQMLRLamide) purified from the central nervous systems of the sea hare, Aplysia Kurodai, using the anterior byssus retractor muscle (ABRM) of the mussel, Mytilus edulis. The amino acid sequences of MMA and MME were the same as those of the myomodulin family peptide found in other mollusks. In this study, we synthesized MME, its derivatives, and other neuropeptides to investigate the relationship between the structure and biological activity of MME. The primary structures of MME’s derivatives, Des[Gly¹]-MME, Des[Gly¹,Leu²]-MME, and Des[Gly¹,Leu²,Gln³]-MME, were LQMLRLamide, QMLRLamide, and MLRLamide, respectively. MMA and synthetic peptides were tested on ABRM in M. edulis as well as muscle preparations in Achatina fulica. MME displayed an inhibitory effect on phasic contraction of the ABRM at 1×10<SUP>-9</SUP> M or higher. MME also had a relaxing effect on the catch-tension of AMRM at 1×10<SUP>-8</SUP> M. Both MMA and its analogs stimulated a contractile response on the crop and relaxed the catch-relaxing response on the penial retractor muscle of A. fulica. These results suggest that MME and its analogs have modulatory effects on various muscles of mollusks. This study has also laid the groundwork for future neural and circuit modulation studies during animal behavioral changes.

군소 ( Aplysia kurodai ) 의 중추신경계로부터 myomodulin A 와 E 의 정제

김찬희(Chan Hee Kim),서해점(Hae Jeom Seo),황은영(Eun Young Hwang),김은정(Eun Jung Kim),고혜진(Hye Jin Go),김인혜(In Hye Kim),서정길(Jung Kil Seo),문정혜(Jung Hye Moon),허민도(Min Do Huh),박남규(Nam Gyu Park) 한국수산과학회 2001 한국수산과학회지 Vol.34 No.3

군소의 중추신경계로부터 myomodulin A (MMA)과 myomodlin E (MME)의 정제에 대해 보고하고자 한다. 500마리 군소의 중추신경계를 산조건으로 추출한 후 4종류의 HPLC column system에 적용하여 두가지 신경성 펩타이드를 정제하였다. HPLC 분리과정에서 얻어진 모든 분획의 활성을 조사하기 위하여 bioassay system으로 진주담치 (Mytilis edulis)의 ABRM을 이용하였다. 최종 정제된 신경성 펩타이드는 Edman 분해법을 이용한 아미노산 서열 분석기, 질량분석기와 화학합성을 통해 완전한 일차구조를 밝힐 수 있었다. 정제한 MMA와 MME의 일차구조는 각각 Pro-Met-Ser-Met-Leu-Arg-Leu-NH₂(847.41 Da)과 Gly-Leu-Gln-Met-Leu-Arg-Leu-NH₂, (830.50 Da) 이었다. 또한 이들 합성 펩타이드는 진주담치의 ABRM에 대해 phasic contration의 조절활성을 나타내었다. This paper reports the purification of myomodulin A (MMA) and myomodulin E (MME) from the sea hare. The central nervous systems of 500 sea hare were extracted in an acidified solvent, after which four HPLC column systems were used to obtain pure peptides. The phasic contraction bioassay using a Mytilus edulis anterior byssus retractor muscle (ABRM) was applied to monitor all collected fractions. The pure peptides were submitted to Edman degradation based automated microsequencing. Mass spectrometry and chemical synthesis confirmed the sequence. The primary structures of MMA and MME were Pro-Met-Ser-Met-Leu-Arg-Leu-NH₂(847.41 Da) and Gly-Leu-Gln-Met-Leu-Arg-Leu-NH₂(830.50 Da), respectively. Synthetic peptides showed a modulating activity of phasic contraction in the ABRM of Mytilus edulis.