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영구치 치수 기질세포를 이용한 연골 분화 및 분화 시기에 따른 형태학적 변화
정주령,김하나,박열,김민정,오영주,신수정,최윤정,김경호 大韓齒科保存學會 2012 Restorative Dentistry & Endodontics Vol.37 No.1
Objectives: The aim was to confirm the stem cell-like properties of the dental pulp stromal cells and to evaluate the morphologic changes during in vitro chondrogenesis. Materials and Methods: Stromal cells were outgrown from the dental pulp tissue of the premolars. Surface markers were investigated and cell proliferation rate was compared to other mesenchymal stem cells. Multipotency of the pulp cells was confirmed by inducing osteogenesis, adipogenesis and chondrogenesis. The morphologic changes in the chondrogenic pellet during the 21 day of induction were evaluated under light microscope and transmission electron microscope. TUNEL assay was used to evaluate apoptosis within the chondrogenic pellets. Results: Pulp cells were CD90, 105 positive and CD31, 34 negative. They showed similar proliferation rate to other stem cells. Pulp cells differentiated to osteogenic, adipogenic and chondrogenic tissues. During chondrogenesis, 3-dimensional pellet was created with multi-layers, hypertrophic chondrocyte-like cells and cartilage-like extracellular matrix. However, cell morphology became irregular and apoptotic cells were increased after 7 day of chondrogenic induction. Conclusions: Pulp cells indicated mesenchymal stem cell-like characteristics. During the in vitro chondrogenesis, cellular activity was superior during the earlier phase (within 7 day) of differentiation.
Efficacy of A/H1N1/2009 split inactivated influenza A vaccine (GC1115) in mice and ferrets
Hae Jung Han,Min-Suk Song,Su-Jin Park,Han Yeul Byun,Norbert John C. Robles,Suk-Hoon Ha,Young-Ki Choi 한국미생물학회 2019 The journal of microbiology Vol.57 No.2
To evaluate the efficacy of a non-adjuvant A/H1N1/2009 influenza A vaccine (GC1115), we demonstrated the immunogenicity and protective efficacy of GC1115 in mouse and ferret models. The immunogenicity of GC1115 was confirmed after intramuscular administration of 1.875, 3.75, 7.5, and 15 μg hemagglutinin antigen (HA) in mice and 7.5, 15, and 30 μg HA in ferrets at 3-week intervals. A single immunization with GC1115 at HA doses > 7.5 μg induced detectable seroconversion in most mice, and all mice given a second dose exhibited high antibody responses in a dose-dependent manner. The mice in the mock (PBS) and 1.875 μg HA immunized groups succumbed by 13 days following A/California/ 04/09 infection, while all mice in groups given more than 3.75 μg HA were protected from lethal challenge with the A/California/04/09 virus. In ferrets, although immunization with even a single dose of 15 or 30 μg of HA induced detectable HI antibodies, all ferrets given two doses of vaccine seroconverted and exhibited HI titers greater than 80 units. Following challenge with A/California/04/09, the mock (PBS) immunized ferrets showed influenza-like clinical symptoms, such as increased numbers of coughs, elevated body temperature, and body weight loss, for 7 days, while GC1115- immunized ferrets showed attenuated clinical symptoms only for short time period (3–4 days). Further, GC1115-immunized ferrets displayed significantly lower viral titers in the upper respiratory tract (nasal cavity) than the mock vaccinated group in a dose-dependent manner. Taken together, this study demonstrates the immunogenicity and protective efficacy of GC1115 as a non-adjuvanted vaccine.
Park Jin-Kyu,Jin Sung-Ha,Park Kum-Hee,Ko Ji-Hun,Ki yeul Nam,Yang Deok-Chun,Park Eun-Kyung 한국자원식물학회 2000 한국자원식물학회 학술심포지엄 Vol.2000 No.-
Coleus forskohlii Briq. of the family Lamiaceae yields a valuable secondary metabolite known as forskolin which is a labdane diterpenoid. Coleus forskohlii is the only known source of this compound. Forskolin is used in medicine for the treatment of glaucoma, congestive cardiomyopathy and asthma. Morphogenic callus was induced from young leaves on MS medium augmented with NAA and BA. These calli, when subcultured on MS with KN alone gave rise to shoots. The regenerated shoot developed good root system on MS medium fortified with NAA. The fully grown plantlets were transferred to soil for acclimatization. Coleus plant is mainly infected by a fungi Lasiodiplodia theobromae which causes root of disease. The fungal culture filterate (FCF) of Lasiodiplodia theobromae, has been used in regeneration media to find the MIC and further to select resistant plants to the pathogen. In the present study 40% FCF in the medium showed maximum inhibition and is there fore considered as the MIC level of Coleus forskohlii. This data could prove to be useful for the future for selecting a resistant C,forskohlii plant against the root disease caused by L.theobromae.
Gd-DOTA Conjugate of RGD as a Potential Tumor-Targeting MRI Contrast Agent
Park, Ji-Ae,Lee, Jae-Jun,Jung, Jae-Chang,Yu, Dae-Yeul,Oh, Chilhwan,Ha, Seunghan,Kim, Tae-Jeong,Chang, Yongmin WILEY-VCH Verlag 2008 Chembiochem Vol.9 No.17
<B>Graphic Abstract</B> <P>Tumor recognition: Conjugation of 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) with the cyclic pentapeptide c(RGDYK) and subsequent complexation with GdCl<SUB>3</SUB>⋅6 H<SUB>2</SUB>O afforded Gd-DOTA-RGD, which, although a single molecular entity, is an efficient, target-specific MRI contrast agent for the α<SUB>ν</SUB>β<SUB>3</SUB> receptors in hepatocellular carcinoma in H-ras12V transgenic mice. The compound displays very low cytotoxicity and might hence warrant further study for clinical usage. <img src='wiley_img/14394227-2008-9-17-CBIC200800529-content.gif' alt='wiley_img/14394227-2008-9-17-CBIC200800529-content'> </P>
Influence of Ginsenosides on the Kainic Acid-Induced Seizure Activity in Immature Rats
Park, Jin-Kyu,Jin, Sung-Ha,Choi, Keum-Hee,Ko, Ji-Hun,Baek, Nam-In,Choi, Soo-Young,Cho, Sung-Woo,Choi, Kang-Ju,Nam, Ki-Yeul Korean Society for Biochemistry and Molecular Biol 1999 Journal of biochemistry and molecular biology Vol.32 No.4
We studied the effects of ginsenosides in immature rats based upon the previous results that ginseng has a suppressive or anticonvulsive activity. To examine the suppressive effect of ginsenosides on kainic acid-induced seizures, the severities and frequencies were observed for 4 h after injection of kainic acid (KA; i.p., 2 mg/kg b.w.) using 10-day-old male Sprague-Dawley rats ($22{\pm}2\;g$). Protopanaxadiol saponins such as ginsenoside-Rb1 (Rb1), ginsenoside-Rb2 (Rb2), ginsenoside-Rc (Rc), and ginsenoside-Rd(Rd) generally reduced the seizure activities while protopanaxatriol saponins such as ginsenoside-Rg1 (Rg1) and ginsenoside-Re (Re) rather increased stereotypic "paddling-like" movements. When vinyl-GABA (v-G) was injected together with Rb1 or Rc, KA-induced seizure severities were additionally reduced only by the injection of Rc, but not by Rb1. The level of gamma isozyme of protein kinase C (PKC-${\gamma}$) in the hippocampus increased about three times as much as that of normal rats at 4 h after KA injection. The increased level of PCK-${\gamma}$ by KA was significantly reduced to about 35% by the coinjection with v-G alone, but it was not changed by v-G together with Rb1 or Rc. The increased level of PKC-${\gamma}$ at 4 h after injection of KA was not consistent with the reduction of seizure severities between Rb1 and Rc. These results suggest that Rc and Rb1 may reduce seizure severity independent of PKC-${\gamma}$ levels, and Rc may additionally act with v-G regarding the GABA metabolism during the stage of KA-induced seizures in the immature rats.
해상에서의 원유 확산 과정 예측을 위한 격자 기반 이산 사건 및 이산 시간 시뮬레이션
하솔(Sol Ha),차주환(Ju-Hwan Cha),박광필(Kwang-Phil Park),조윤옥(Yoon-Ok Cho),이준채(Jun-Chae Lee),이규열(Kyu-Yeul Lee) (사)한국CDE학회 2011 한국 CAD/CAM 학회 학술발표회 논문집 Vol.2011 No.1
본 논문에서는 해상 환경에서 원유 유출 시 원유의 확산 과정을 빠르게 시뮬레이션 할 수 있는 oil spreading 시뮬레이션 모델을 제안하였다. 해상 환경을 규칙적인 격자 형태의 cell 로 표현하고, 각 cell 은 cell 내에 포함된 원유의 양, 수온, 해류와 바람의 방향과 같은 정보를 포함한다. 각 cell은 “깨끗함(clean)”과 “오염됨(polluted)”의 2 가지 상태가 정의되어 있으며, cell 내의 원유는 정의된 규칙에 따라 이웃 cell 로 확산된다. 격자 형태의 Cell 은 3 개의 원유 확산 규칙을 가지고 있다. 첫째, cell 내에 포함된 원유는 상대적으로 적은 원유를 가진 이웃 cell 로 이동한다. 둘째, cell 내에 포함된 원유는 일정 시간마다 cell 의 수온에 비례하는 양만큼 증발한다. 셋째, 해류의 방향과 속도, 바람의 방향과 속도에 영향을 받는다. 이러한 cell 의 규칙과 cell 에 포함되는 정보를 기반으로 하나의 cell 에 대한 oil spreading 시뮬레이션 모델을 이산 사건 및 이산 시간 혼합형 시뮬레이션 모델 구조를 이용하여 정의하였다. 그리고 300(m) X 300(m) 크기의 해상 공간에서 10,000kL 의 원유가 유출되었을 때를 가정하여 바람과 해수의 방향을 변경하면서 원유 유출 시뮬레이션을 수행하여 정의한 oil spreading 시뮬레이션 모델의 기능을 확인하였다.
Nguyen, Manh Ha,Kim, Dae Ho,Park, Ji Hyun,Park, Young Ui,Lee, Moo Yeul,Choi, Myeong Hee,Lee, Dong Ho,Lee, Jong Kyu Institute of Forest Science 2021 Journal of Forest Science Vol.37 No.1
Decay fungi can decompose plant debris to recycle carbon in the ecosystem. Still, they can also be fungal pathogens, which can damage living trees and/or wood material and cause a large amount of timber loss. We isolated and identified basidiomycetous fungi from the decayed bark of Mongolian oak wrapped with sticky roll traps. The degrading enzyme activities were then tested for all fungal isolates. The decay ability of selected isolates was assessed based on the weight loss of wood discs after inoculating with culture suspension of decay fungi under the different humidity levels. A total of 46 basidiomycetous fungal isolates belonged to 12 species, and 10 genera were obtained from Jong Myo (16 isolates), Chang Kyung palace (7 isolates), Cheong Gye (10 isolates), and Gun Po (13 isolates). Gymnopus luxurians was the most dominant fungus in the present study, and this species distributed in all survey sites with 9 isolates in Jong Myo, followed by 3 isolates in Chang Kyung palace, while Cheong Gye and Gun Po had only 1 isolate each. Among 46 isolates, 44 isolates secreted at least one enzyme, while 25 isolates produced both cellulase and phenol oxidase enzymes, and 2 isolates produced neither. The assessment of decay ability by artificial inoculation indicated that the weight loss of wood discs was significantly influenced by humidity conditions when inoculated with bark decay fungi. The percent weight losses by G. luxurians inoculation in RH of 90-100% and RH of 65-75% were 4.61% and 2.45%, respectively. The weight loss caused by Abortiporus biennis were 6.67% and 0.46% in RH of 90-100% and RH of 45-55%, respectively. The humidity reduction approach should be applied for further studies to control the growth and spread of bark decay fungi on the trunks wrapped with sticky roll traps.