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Kim, J.Y.,Kim, K.B.,Son, H.J.,Chae, Y.C.,Oh, S.T.,Kim, D.W.,Pak, J.H.,Seo, S.B. North-Holland Pub ; Elsevier Science Ltd 2012 FEBS letters Vol.586 No.19
Significant progress has been made in understanding the relationship between histone modifications and 'reader' molecules and their effects on transcriptional regulation. A previously identified INHAT complex subunit, SET/TAF-Iβ, binds to histones and inhibits histone acetylation. To investigate the binding specificities of SET/TAF-Iβ to various histone modifications, we employed modified histone tail peptide array analyses. SET/TAF-Iβ strongly recognized PRC2-mediated H3K27me½/3; however, the bindings were completely disrupted by H3S28 phosphorylation. We have demonstrated that SET/TAF-Iβ is sequentially recruited to the target gene promoter ATF3 after the PRC2 complex via H3K27me recognition and may offer additive effects in the repression of the target gene.
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Yi, Y.J.,Ko, H.J.,Lee, S.H.,Yang, C.B.,Son, D.S.,Kim, H.K.,Park, C.S. 충남대학교 형질전환복제돼지연구센터 2004 논문집 Vol. No.8
This study was carried out to investigate in vitro fertilization and development of in vitro matured pig oocytes inseminated with the Duroc boar sperm by different sperm washing media after thawing of the 5 ml frozen straws. Immature follicular oocytes (30-40) were transferred into each well of a Nunc 4-well multidish containing 500㎕ mTCM 199 maturation medium. The sperm rich portion of ejaculates was collected into a 250 ml insulated vacuum bottle and gradually cooled 22 to 24℃ over a 2 h period. Semen was centrifuged at 800g for 10 min and the seminal Plasma discarded. Sperm were resuspended in a lactose-egg yolk and N-acetyl-D-glucosamine (LEN) diluent to contain 1×10^9 sperm/ml and cooled to 5℃ over a 2 h period. Immediately before freezing, semen was rediluted with an equal volume of LEN+4% glycerol and packed Into 5 ml straws. After thawing of the 5 ml straw, the 5 ml semen was diluted with 20 ml Beltsville thawing solution (BTS) at room temperature. Oocγtes were inseminated with untreated (unwashed and nonpreincubated) or treated sperm (washed two times in BTS, mTLP-PVA and mTBM media, respectively and nonpreincubated) with 2×10^7 sperm concentration. Oocytes were coincubated for 6 h in 500㎕ mTBM fertilization. At 6 h after IVF, oocytes were transferred Into 500㎕ NCSU-23 culture medium for further culture of 6 h. Sperm penetration, polyspermy and male pronuclear formation of oocytes at 12 h after IVF and developmental ability of oocytes at 48 h after IVF were evaluated. Sperm penetration rate, male pronuclear formation and rate of cleaved embryos were higher in the BTS, mTLP-PVA and mTBM treatments than the unwashed treatment (p<0.05). The rate of blastocysts from the cleaved oocytes (2-4 cell stage) were higher in the mTLP-PVA treatment than in the unwashed, BTS and mTBM treatments. In conclusion, we recommend the washing of frozen-thawed sperm with mTLP-PYA medium before in vitro fertilization of oocytes in mTBM medium.
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Jin, Y.C.,Kim, C.W.,Kim, Y.M.,Nizamutdinova, I.T.,Ha, Y.M.,Kim, H.J.,Seo, H.G.,Son, K.H.,Jeon, S.J.,Kang, S.S.,Kim, Y.S.,Kam, S.C.,Lee, J.H.,Chang, K.C. North-Holland ; Elsevier Science Ltd 2009 european journal of pharmacology Vol.614 No.1
The aim of the present study was to evaluate the protective effect of cryptotanshinone (CTS), one of active ingredients of Salvia miltiorrhiza root, on myocardial ischemia-reperfusion injury in rat due to inhibition of some inflammatory events that occur by NF-kB-activation during ischemia and reperfusion. Myocardial ischemia and reperfusion injury was induced by occluding the left anterior descending coronary artery for 30 min followed by either 2 h (biochemical analysis) or 24 h (myocardial function and infarct size measurement) reperfusion. CTS injected (i.v.) 10 min before ischemia and reperfusion insult. CTS significantly reduced the infarct size and improved ischemia and reperfusion-induced myocardial contractile dysfunction. Furthermore, CTS inhibited NF-kB translocation, expression of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), neutrophil infiltration and MPO activity in ischemic myocardial tissues. CTS also significantly reduced plasma levels of TNF-α, IL-1β due to ischemia and reperfusion. Interestingly, H<SUB>2</SUB>O<SUB>2</SUB>-stimulated NF-kB-luciferase activity and TNF-α-induced expression of vascular cell adhesion molecule-1 (VCAM-1) and intracellular adhesion molecule-1 (ICAM-1) expressions in human umbilical vein endothelial cells (HUVEC) were significantly inhibited by CTS. Taken together, it is concluded that CTS may attenuate ischemia and reperfusion-induced microcirculatory disturbances by inhibition of proinflammatory cytokine production, reduction of neutrophil infiltration and possibly inhibition of adhesion molecules through inhibition of NF-kB-activation during ischemia and reperfusion.
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Effects of Exogenous Enzymes on Ruminal Fermentation and Degradability of Alfalfa Hay and Rice Straw
Yang, H.E.,Son, Y.S.,Beauchemin, K.A. Asian Australasian Association of Animal Productio 2011 Animal Bioscience Vol.24 No.1
This study was conducted to evaluate the use of exogenous enzymes as a potential means of improving the ruminal digestion (i.e., degradability) of alfalfa hay and rice straw. Twenty six enzyme-additives were examined in terms of protein concentration and enzymic activities on model substrates. The exogenous enzymes contained ranges of endoglucanase, xylanase, ${\beta}$-glucanase, ${\alpha}$-amylase, and protease activities. Six of the enzyme additives were chosen for further investigation. The enzyme additives and a control without enzyme were applied to mature quality alfalfa hay substrate and subsequently incubated in rumen batch cultures. Five of the enzyme additives (CE2, CE13, CE14, CE19, and CE24) increased total gas production (GP) at 48 h of incubation compared to the control (p<0.05). The two additives (CE14 and CE24) having the greatest positive effects on alfalfa hay dry matter, neutral detergent fibre (NDF) and acid detergent fibre (ADF) degradability were further characterized for their ability to enhance degradation of low quality forages. The treatments CE14, CE24, a 50:50 combination of CE14 and CE24 (CE14+24), and control (no enzyme) were applied to mature alfalfa hay and rice straw. For alfalfa hay, application of the two enzyme additives, alone and in combination, increased GP compared to the control at 48 h fermentation (p<0.05), whereas only CE14 and CE14+24 treatments improved GP from rice straw (p<0.05). Rumen fluid volatile fatty acid concentrations throughout the incubation of rice straw were analyzed. Acetate concentration was slightly lower (p<0.05) for CE14${\times}$CE24 compared to the control, although individually, CE14 and CE24 acetate concentrations were not different from the control. Increases (p<0.05) in alfalfa hay NDF degradability measured at 12 and 48 h of incubation occurred only for CE14 (at 12 h) and for CE14+24 (at 12 and 48 h). Similarly, ADF degradability increased (p<0.05) with CE14 and CE14+24. As for rice straw, increased DM degradability was observed at 12 and 48 h of incubation for all enzyme treatments with an exception for CE14 at 12 h. The degradability of NDF was improved by all the enzyme treatments at either incubation time, while ADF degradability was only enhanced at 48 h. Overall, the enzymes led to enhanced digestion of mature alfalfa and there was evidence of improved digestibility of rice straw, an even lower quality forage.
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Kim, Y. S.,Yoo, A.,Son, J. W.,Kim, H. Y.,Lee, Y. J.,Hwang, S.,Lee, K. Y.,Lee, Y. J.,Ayata, C.,Kim, H. H. Humana Press 2017 Molecular Neurobiology Vol. No.
<P>Phosphatidylinositol 3-kinases (PI3Ks) have recently been implicated in apoptosis and ischemic cell death. We tested the efficacy of early intervention with a peptide PI3K activator in focal cerebral ischemia. After determining the most effective dose (24 mu g/kg) and time window (2 h after MCAO) of treatment, a total of 48 rats were subjected to middle cerebral artery occlusion (MCAO). Diffusion weighted MRI (DWI) was performed 1 h after MCAO and rats with lesion sizes within a predetermined range were randomized to either PI3K activator or vehicle treatment arms. Fluid attenuated inversion recovery (FLAIR) MRI, neurological function, western blots, and immunohistochemistry were blindly assessed. Initial DWI lesion volumes were nearly identical between two groups prior to treatment. However, FLAIR showed significantly smaller infarct volumes in the PI3K activator group compared with vehicle (146 +/- 81 mm(3) and 211 +/- 96 mm(3), p = 0.045) at 48 h. The PI3K activator group also had better neurological function for up to 2 weeks. In addition, PI3K activator decreased the number of TUNEL-positive cells in the peri-infarct region compared with the control group. Western blot and immunohistochemistry showed increased expression of phosphorylated Akt (Ser473) and GSK-3 beta (Ser9) and decreased expression of cleaved caspase-9 and caspase-3. Our results suggest a neuroprotective role of early activation of PI3K in ischemic stroke. The use of DWI in the randomization of experimental groups may reduce bias.</P>
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Yi, Y.J.,Ko, H.J.,Lee, S.H.,Yang, C.B.,Son, D.S.,Kim, H.K.,Park, C.S. Asian Australasian Association of Animal Productio 2004 Animal Bioscience Vol.17 No.2
This study was carried out to investigate in vitro fertilization and development of in vitro matured pig oocytes inseminated with the Duroc boar sperm by different sperm washing media after thawing of the 5 ml frozen straws. Immature follicular oocytes (30-40) were transferred into each well of a Nunc 4-well multidish containing $500{\mu}l$ mTCM199 maturation medium. The sperm rich portion of ejaculates was collected into a 250 ml insulated vacuum bottle and gradually cooled 22 to $24^{\circ}C$ over a 2 h period. Semen was centrifuged at 800 g for 10 min and the seminal plasma discarded. Sperm were esuspended in a lactose-egg yolk and N-acetyl-Dglucosamine (LEN) diluent to contain $1{\times}10^{9}$ sperm/ml and cooled to $5^{\circ}C$ over a 2 h period. Immediately before freezing, semen was rediluted with an equal volume of LEN+4% glycerol and packed into 5 ml straws. After thawing of the 5 ml straw, the 5 ml semen was diluted with 20 ml Beltsville thawing solution (BTS) at room temperature. Oocytes were inseminated with untreated (unwashed and nonpreincubated) or treated sperm (washed two times in BTS, mTLP-PVA and mTBM media, respectively and nonpreincubated) with $2{\times}10^{7}$ sperm concentration. Oocytes were coincubated for 6 h in $500{\mu}l$ mTBM fertilization. At 6 h after IVF, oocytes were transferred into $500{\mu}l$ NCSU-23 culture medium for further culture of 6 h. Sperm penetration, polyspermy and male pronuclear formation of oocytes at 12 h after IVF and developmental ability of oocytes at 48 h after IVF were evaluated. Sperm penetration rate, male pronuclear formation and rate of cleaved embryos were higher in the BTS, mTLP-PVA and mTBM treatments than the unwashed treatment (p<0.05). The rate of blastocysts from the cleaved oocytes (2-4 cell stage) were higher in the mTLP-PVA treatment than in the unwashed, BTS and mTBM treatments. In conclusion, we recommend the washing of frozen-thawed sperm with mTLP-PVA medium before in vitro fertilization of oocytes in mTBM medium.
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Bang, J.,Choi, M.,Son, H.,Beuchat, L.R.,Kim, Y.,Kim, H.,Ryu, J.H. Elsevier Science Publishers 2016 International journal of food microbiology Vol.237 No.-
<P>Sanitizing radish seeds intended for edible sprout production was achieved by applying simultaneous treatments with gaseous chlorine dioxide (ClO2), high relative humidity (RH, 100%), and mild heat (55 degrees C). Gaseous ClO2 was produced from aqueous ClO2 (0.66 ml) by mixing sulfuric acid (5% w/v) with sodium chlorite (10 mg/mL) in a sealed container (1.8 L). Greater amounts of gaseous ClO2 were measured at 23% RH (144 ppm after 6 h) than at 100% RH (66 ppm after 6 h); however, the lethal activity of gaseous ClO2 against naturally occurring mesophilic aerobic bacteria (MAB) on radish seeds was significantly enhanced at 100% RH. For example, when exposed to gaseous ClO2 at 23% RH, the number of MAB on radish seeds decreased from 3.7 log CFU/g to 2.6 log CFU/g after 6 h. However, when exposed to gaseous ClO2 at 100% RH for 6 h, the MAB population decreased to 0.7 log CFU/g after 6 h. Gaseous ClO2 was produced in higher amounts at 55 degrees C than at 25 degrees C, but decreased more rapidly over time at 55 degrees C than at 25 degrees C. The lethal activity of gaseous ClO2 against MAB on radish seeds was greater at 55 degrees C than at 25 degrees C. When radish seeds were treated with gaseous ClO2 (peak concentration: 195 ppm) at 100% RH and 55 degrees C, MAB were reduced to populations below the detectable level (<-0.7 log CFU/g) within 2 h without decreasing the seed germination rate (97.7%). The lethality of combined treatments against artificially inoculated Escherichia coli O157:H7 was also evaluated. When exposed to gaseous ClO2 at 100% RH and 55 degrees C for 6 h, the initial number of E. coli O157:H7 (3.5 log CFU/g) on radish seeds decreased to below the detection limit (0.7 log CFU/g) by direct plating but it was not eliminated from seeds. The germination rate of radish seeds was not significantly (P> 0.05) decreased after treatment for 6 h. The information reported here will be useful when developing decontamination strategies for producing microbiologically safe radish seed sprouts. (C) 2016 Published by Elsevier B.V.</P>
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학령전기 아동 부모의 성지식, 성교육 인식 및 현황, 성교육 요구도에 관한 연구
김다연,김민지,김유정,방예림,손윤희,윤지현,조아람,조은미,최지현,Jubilie Hta 이화여자대학교 간호과학대학 2013 이화간호학회지 Vol.- No.47
Purpose: The purpose of this study was to determine the sexual knowledge, and sex education perception and current status of the parents of preschoolers. The objective was to analyze the relationship between sexual knowledge and sex education demands of parents. Method: The method used in this study was a descriptive correlationstudy. The subjects were 278 parents of 3-to-6 year old children in 5 kindergartens located in Seoul. Data were analyzed using frequency, percentage, average, standard deviation, t-test, ANOVA, Scheffe test, and correlation using the SPSS 20.0 program. Result: The results of this study were the following: 1.There was no significantdifference in the sexual knowledge between the father and mother of preschoolers (Father 20.76, Mother 20.73). According to the findings, sexual knowledge was higher when both father and mother were older (P=.005). Similarly, when the economic level of the father and mother was high, sexual knowledge was also high (P=.030). 2.Concerning sexual education perception, it was found out that both father and mother thought that sex education was necessary. 3.In terms of sex education, a significant difference was noted. Mothers conducted sex education more often than fathers (Fathers, 46.7% Mother, 60.3%). Both the father and mother thought that providing sex education would be best in Grades 1-2, especially for ages 6-7. 4.When asked about the demands for sex education in terms of the physical, social, and living aspects, living aspect was the highest demand. It was revealed that both fathers and mothers were aware of the need for sex education, and when parents hadmore sexual knowledge, they would demand more sex education. Accordingly, there was a correlation between parent’s sexual knowledge and demand for sex education (r=.202, p=.001). Conclusion: Since the age,knowledge, education and economic levels of parents were different, it was necessary to develop sexeducation programs in accordance with their levels. In this way, preschooler parents would be able to teach sex education appropriate to their children’s level.
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