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Gung Pyo Lee 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07
Recently, many breeders have preferred to use molecular markers for introgression backcross programs enabling foreground and background selection to cope with rapid cultivar changing of seed markets. In accumulation of target traits with marker-assisted selection, larger numbers of markers should give better resolution. For the analysis of quantitative traits, a high-density genetic map with a large number of markers is required for discovering more accurately linked markers with traits. Watermelon is a recalcitrant plant to generate a high-density genetic map with conventional molecular markers including simple sequence repeats (SSRs), since watermelon has narrow genetic diversity background and severe segregation distortions of those SSR markers. Thus, we have developed efficient and valid way to assemble genetic map and markers by next-generation sequencing coupled with genotyping by sequencing in F2 generation. After crosses between Citurullus lanatus ssp. citroides (PI254744 and PI189225) and C. lanatus ssp. lanatus (TS34, Korean cultigen), 163 of F2 progeny were sequenced through Illumina's Hi-Seq GAII platform. From sequence information of those variant call files, the SNPs were indexed and filtered by sequencing depth with genotype converter (SNP Genotyper), and optimized by heuristic physical bin mapping to construct more reliable genetic linkage map. Reliable SNP loci were determined and compared to sequences of physical reference map. Using the genetic map, we determined QTLs in F2:3 population and found major loci corresponding to seed size and powdery mildew race1 resistance in watermelon.
Effect of GeO₂ Concentration on Hydroponically-Grown Lettuce (Lactuca sativa)
Gung Pyo Lee,Hee-Seung Park,Jae Hee Won,Kuen Woo Park 한국원예학회 2005 Horticulture, Environment, and Biotechnology Vol.46 No.2
Various concentrations of germanium dioxide (GeO₂) was treated such as 1.0, 2.0, 4.0, 6.0, and 8.0 ㎎ㆍL?¹ to lettuce (Lactuca sativa cv. Cheongchima) cultured in hydoponics for 4 weeks. Ge was linearly accumulated in the plants, primarily in the roots, as GeO₂ concentration in the nutrient solution increased from 1.0 to 8.0 ㎎ㆍL?¹. In addition, Ge once taken by the root was hardly moved to the shoot. The plant showed the best growth at 4.0 ㎎ㆍL?¹ GeO₂ for 7 days. In all ranges of the Ge treatment, the plant showed no phenotypic changes in leaf shape and color including necrosis or chlorosis, but the roots were stunted and thickened. The roots treated with GeO₂ at 6 or 8 ㎎ㆍL?¹ showed thickened cortex, but they had similar sizes of cells and vascular tissues. The leaf tissues treated with GeO₂ had denser mesophyll cells than the control. Interestingly, the Ge-treated leaf looked harder, more greenish, and more glossy than the control. With increasing GeO₂ concentration in the nutrient solution, the root activity was decreased, whereas lipid peroxidation increased.
Factors Affecting on Regeneration in 'WHANGKEUMBAE' Pear (Pyrus pyrifolia)
Gung-Pyo Lee 경상대학교 농업생명과학연구원 2009 농업생명과학연구 Vol.43 No.6
Combinations of plant growth regulators, darkness treatments, and the order of expanding leaves for explants were evaluated for optimizing in vitro shoot regeneration rate of 'Whangkeumbae' pear. In a MS medium, supplemented with 8.88 μM 6-benzylaminopurine (BA) and 0.49 μM indole-3-butyric acid (IBA), green foci were observed on the surface of the callus 8 days after culture initiation. Some adventitious buds were later induced from those green foci, resulting in the appearance of normal shoots. In a medium containing 22.20 μM BA, the surface of the callus became compact and greenish, and many adventitious buds were formed over the entire area of the callus surface. When comparing BA concentration via histological observation, the section which had been treated with 22.20 μM BA exhibited closer cell aggregation than those with 8.88 μM BA. The darkness treatment enhanced the formation of adventitious shoots for up to 3 weeks. The youngest two expanding leaves, proximal to the shoot apex, were proved to be the most regenerative, and yielded the highest shoot number per regenerating leaf. A fourth strength MS medium, which was supplemented with 0.54 μM NAA, yielded good quality plantlets, with regard to root number and root length.
Lee, Ju-Suk,Kim, Ye-Jin,Ryu, Ki-Hyun,Han, Tae-Ho,Park, Kuen-Woo,Chung, Kyu-Hwan,Lee, Chan,Lee, Gung-Pyo,Kim, Sun-Hyung,Hong, Jin-Sung,Park, Young-Doo,Woo, Eun-Taeck,Park, Sung-Chul,Son, Dae-Yeul 한국원예학회 2012 Horticulture, Environment, and Biotechnology Vol.53 No.2
Pollen of genetically modified (GM) pepper containing the gene for cucumber mosaic virus (CMV) coat protein (CP) and GM Chinese cabbage with high phenylethylisothiocyanate (PEITC) content was investigated for assessment of allergic risk. Amino acid (AA) sequences of the inserted gene products of GM virus resistant pepper and GM Chinese cabbage with high PEITC content were compared with those of known allergens. No known allergen greater than 35% AA sequence homology, over 80 AA window or more than 8 consecutive identical AA was found. Protein patterns of GM/non-GM pepper and Chinese cabbage pollen extracts in SDS-PAGE analysis showed the same distribution of protein bands among the GM and non-GM pepper or Chinese cabbage, respectively. Sera from pollen allergic patients showed some IgE reactivity via immunoblotting and ELISA; however, no differences were observed between the pollen of GM and non-GM pepper or Chinese cabbage, respectively. Based on these results, we conclude that pollens of the virus resistant GM pepper and GM Chinese cabbage with high PEITC have no differences in their protein composition or allergenicity relative to non-GM pepper and Chinese cabbage.
Lee, Gung-Pyo,Ryu, Ki-Hyun,Kim, Hyun-Ran,Kim, Chung-Sun,Lee, Dong-Woo,Kim, Jeong-Soo,Park, Min-Hye,Noh, Young-Mi,Choi, Sun-Hee,Han, Dong-Hyun,Lee, Chang-Hoo The Korean Society of Plant Pathology 2002 Plant Pathology Journal Vol.18 No.5
Apple mosaic virus (ApMV), a member of the genus Ilarvirus, was detected and isolated from diseased 'Fuji' apple (Malus domestica) in Korea. The coat protein (CP) genes of two ApMV strains, denoted as ApMV-Kl and ApMV-K2, were amplified by using the reverse transcription and polymerase chain reaction (RT-PCR) and were analyzed thereafter. The objectives were to define the molecular variability of genomic information of ApMV found in Korea and to develop virus-derived resistant gene source for making virus-resistant trans-genic apple. RT-PCR amplicons for the APMVS were cloned and their nucleotide sequences were determined. The CPs of ApMV-Kl and ApMV-K2 consisted of 222 and 232 amino acid residues, respectively. The identities of the CPs of the two Korean APMVS were 93.1% and 85.6% at the nucleotide and amino acid sequences, respectively. The CP of ApMV-Kl showed 46.1-100% and 43.2-100% identities to eight different ApMV strains at the nucleotide and amino acid levels, respectively. When ApMV-PV32 strain was not included in the analysis, ApMV strains shared over 83.0% and 78.6% homologies at the nucleotide and amino acid levels, respectively. ApMV strains showed heterogeneity in CP size and sequence variability. Most of the amino acid residue differences were located at the N-termini of the strains of ApMV, whereas, the middle regions and C-termini were remarkably conserved. The APMVS were 17.(1-54.5% identical with three other species of the genus Ilarviyus. ApMV strains can be classified into three subgroups (subgroups I, II, and III) based on the phylogenetic analysis of CP gene in both nucleotide and amino acid levels. Interestingly, all the strains of subgroup I were isolated from apple plants, while the strains of subgroups II and III were originated from peach, hop, or pear, The results suggest that ApMV strains co-evolved with their host plants, which may have resulted in the CP heterogeneity.