국내육성 오차드그라스 품종들의 캘러스 형성율 및 식물체 재분화 효율

김기용,강경민,배은경,이인애,임용우,최기준,박근제,손대영,조진기 Plant molecular biology and biotechnology research 2003 Plant molecular biology and biotechnology research Vol.2003 No.-

Comparisons of callus formation ratios from seed explants, callus sizes, regeneration ratios from callus and regeneration efficiency for 4 orchardgrass (Dactylis glomerata L.) varieties (Three were developed in Korea and one was imported from foreign nation) are as follows; Jangbeol 102 (67.0%) has the highest callus formation ratio in 4 weeks incubated callus after bedding the seed explants, but Potomac (68.4%) has the highest ratio in 6-seek callus. Potomac (3.93cm) has the highest callus size in 4-week callus, but Jangbeol 101 (4.32cm) has the highest size in 6-week callus. Jangbeol 101 (17.7%) has the highest plant regeneration ratio in 4-week callus, but Potomac (37.4%) has the highest raion in 6-week callus. Jangbeol 102 (11.5%) has the highest plant regeneration efficiency in 4-week callus, but Potomac (25.6%) has the highest efficiency in 6-week callus.

'삼국사기'와 '삼국유사' : 역사와 문학의 만남

김기봉,김미애,김혜경 경기대학교 2009 시민인문학 Vol.16 No.-

모든 학문이 추구하는 것은 진실이다. 역사학이 사실서사를 통해 진실을 밝힌다면, 문학은 허구서사를 통해 진실을 드러낸다. 그런데 이 둘 가운데 무엇이 진실로 들어가는 더 큰 문일까? 아리스토텔레스는 󰡔시학󰡕에서 실제로 일어난 일을 이야기하는 역사보다는 일어날 법한 일을 이야기하는 시가 진실에 이르는 더 큰 문이라고 주장했다. 시는 보편적인 것을 말하지만 역사는 개별적인 것을 말하기 때문에 그렇다는 것이다. 아리스토텔레스가 설정한 문학과 역사의 우선순위는 동아시아 서사전통에서는 역전되었다. 예컨대 역사서사인󰡔삼국사기󰡕가 문학서사인󰡔삼국유사󰡕보다 더 진실에 가까운 것으로 여겨졌다. 서사가 서구에서는 허구에서 역사가 분리하는 방향으로 전개됐다면, 동아시아에서는 반대로 역사로부터 허구가 분화하는 방향으로 나갔다. 근대에 이르러 동서양 모두에서 사실과 진실을 일치시키는 근대 사실주의는 허구를 통해 진실로 들어가는 문을 차단했다. 사실주의 문법에 따라 문학이 역사를 모방하는 역사소설과 같은 장르가 생겨났다. 탈근대에서 팩션의 등장은 사실만이 진실이라는 근대 사실주의 문법의 해체를 의미한다. 이 같은 맥락에서 본 논문은 사실과 허구는 진실과 거짓의 등식이 아닌 현실서사와 꿈의 서사로 이해돼야 한다는 것을 󰡔삼국사기󰡕와󰡔삼국유사󰡕에 대한 비교연구를 통해 해명한다. 탈근대주의 문법에 따르면, 󰡔삼국사기󰡕가 역사서사라면󰡔삼국유사󰡕는 팩션이라는 것이 본 논문의 테제다.

과민성 장 증후군의 증상 경감을 위한 비약물적 접근 고찰

최명애,김금순,안경주,채영란,최정안,홍해숙,박미정,이경숙,신기수,정재심 대한기초간호자연과학회 2005 Journal of korean biological nursing science Vol.7 No.2

There have been many trials of clinical efficacy of multicomponent and single component treatments for irritable bowel syndrome(IBS). We reviewed effects of non phamacological treatments in the IBS. Though the efficacy of multi-component approaches was unclear, several results suggest that cognitive behavioral therapy was effective in improving gastrointestinal symptoms of IBS. As a single component, cognitive therapy and relaxation with or without biofeedback could improve the symptoms and psychological health of IBS patients. Yoga, meditation, self-help information and hypnotherapy could be applicable to IBS.

토란의 황화경으로부터 캘러스 형성 및 식물체 재생

윤애화,이지영,임순희,김현정,안장순 全南大學校 農業科學技術硏究所 1998 農業科學技術硏究 Vol.33 No.-

토란의 기지 shoot를 암배양하여 절간이 신장한 황하경을 얻고 이황하경의 절편을 계대배양하여 기내증식을 시도하였다. 황하경은 NAA 0.2mg/ℓ가 첨가된 배지에서 가장 길게 신장하였다. 황하경은 자구형성형이 모구배대형보다 길게 신장하였다. 호르몬이 첨가된 거의 모든 배지에서 protocorm이 형성되었으나 0.2mg/ℓ NAA + 0.2mg/ℓ BA를 첨가한 배지에서 특히 많이 형성되었고 이 protocorm을 MS기본 배지에 옮겼을 때 8.5%의 재분화율을 보였다. Etiolated stems with elongated internodes were obtained from the in vitro culture of shoots of Colocasia esculenta schout under dark condition and plant differntiation was achived by subculturing the ethiolated stem segments. The etiolated stems elongates better on the media containing 0.2mg/ℓNAA than of those lacking it. Subculture of the etiolated stem segments produced protocorm-like structures in all the media examined. The medium containing 0.2mg/ℓNAA and 0.2mg/ℓBA produced the higest number of protocorm-like structure, which regenerated into plantets at 85% when transferred to phytohormone-free MS medium.

Chronic Hepatitis B Infection Is Significantly Associated with Chronic Kidney Disease: a Population-based, Matched Case-control Study

Kim, Sung-Eun,Jang, Eun Sun,Ki, Moran,Gwak, Geum-Youn,Kim, Kyung-Ah,Kim, Gi-Ae,Kim, Do Young,Kim, Dong Joon,Kim, Man Woo,Kim, Yun Soo,Kim, Young Seok,Kim, In Hee,Kim, Chang Wook,Kim, Ho Dong,Kim, Hyun The Korean Academy of Medical Sciences 2018 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.33 No.42

<P><B>Background</B></P><P>Hepatitis B virus (HBV) infection leads to hepatic and extrahepatic manifestations including chronic kidney disease (CKD). However, the association between HBV and CKD is not clear. This study investigated the association between chronic HBV infection and CKD in a nationwide multicenter study.</P><P><B>Methods</B></P><P>A total of 265,086 subjects who underwent health-check examinations in 33 hospitals from January 2015 to December 2015 were enrolled. HBV surface antigen (HBsAg) positive cases (n = 10,048), and age- and gender-matched HBsAg negative controls (n = 40,192) were identified. CKD was defined as a glomerular filtration rate (GFR) < 60 mL/min/1.73 m<SUP>2</SUP> or proteinuria as at least grade 2+ of urine protein.</P><P><B>Results</B></P><P>HBsAg positive cases showed a significantly higher prevalence of GFR < 60 mL/min/1.73 m<SUP>2</SUP> (3.3%), and proteinuria (18.9%) than that of the controls (2.6%, <I>P</I> < 0.001, and 14.1%, <I>P</I> < 0.001, respectively). In the multivariate analysis, HBsAg positivity was an independent factor associated with GFR < 60 mL/min/1.73 m<SUP>2</SUP> along with age, blood levels of albumin, bilirubin, anemia, and hemoglobin A1c (HbA1c). Likewise, HBsAg positivity was an independent factor for proteinuria along with age, male, blood levels of bilirubin, protein, albumin, and HbA1c. A subgroup analysis showed that HBsAg positive men but not women had a significantly increased risk for GFR < 60 mL/min/1.73 m<SUP>2</SUP>.</P><P><B>Conclusion</B></P><P>Chronic HBV infection was significantly associated with a GFR < 60 mL/min/1.73 m<SUP>2</SUP> and proteinuria (≥ 2+). Therefore, clinical concern about CKD in chronic HBV infected patients, especially in male, is warranted.</P>

Hepatitis C Virus Infection Is Associated with Lower Levels of Serum Cholesterol, Triglyceride, and LDL-Cholesterol Level: A Population-Based, Matched Case-Control Study in Korea

( Sung Eun Kim ),( Eun Sun Jang ),( Moran Ki ),( Geum-Youn Gwak ),( Kyung-Ah Kim ),( Gi-Ae Kim ),( Do Young Kim ),( Dong Joon Kim ),( Man Woo Kim ),( Yun Soo Kim ),( Young Seok Kim ),( In Hee Kim ),( 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1

Aims: Hepatitis C virus (HCV) entry and replication is associated with interruption of lipid metabolism. We aimed to investigate the association between anti-HCV positivity and serum lipid profiles in a nationwide, multicenter study. Methods: A total of 268,422 subjects who underwent health-check examination in nationwide 33 hospitals from Jan 2015 to Dec 2015 were enrolled. Data on the anti-HCV positivity, and biochemical laboratory results including serum levels of total cholesterol, triglyceride, LDL-cholesterol, and HDL-cholesterol were obtained and analyzed. Among the subjects, 1,360 anti-HCV antibody positive cases (0.6%), and 5,440 anti-HCV negative controls matched for age and sex to the cases were identified in same dataset. Results: Though body mass index (BMI) was not different between case and control group, the mean serum levels of total cholesterol (185 mg/dL), triglyceride (111.8 mg/dL), LDL cholesterol (117.4 mg/dL), and HDL cholesterol (54.5 mg/dL) were all significantly lower in anti-HCV positive group than in controls (192.5, 119.1, 125.1, and 55.8 mg/dL, respectively). By multivariable analyses using logistic regression, the independent factors associated with elevated serum total cholesterol (≥200 mg/dL) were age, male sex, anti-HCV positivity or HCV-RNA positivity. In addition, the independent factors associated with elevated serum triglyceride(≥150 mg/dL) were age, male sex, BMI (≥25kg/m2) HbA1C (≥5.5%), ALT (≥40IU/L), anti-HCV positivity or HCV-RNA positivity. Moreover, the independent factors associated with elevated LDL-cholesterol (≥130 mg/dL) were age, male sex, and anti-HCV positivity or HCV-RNA positivity. Conclusions: In this large population-based data, HCV infection independently associated with lower serum total cholesterol level, lower triglyceride level and lower LDL cholesterol level.

Hepatitis B Virus Infection Is Significantly Associated with Chronic Kidney Disease: A Population-Based, Matched Case-Control Study in Korea

( Sung Eun Kim ),( Eun Sun Jang ),( Moran Ki ),( Geum-youn Gwak ),( Kyung-ah Kim ),( Gi-ae Kim ),( Do Young Kim ),( Dong Joon Kim ),( Man Woo Kim ),( Yun Soo Kim ),( Young Seok Kim ),( In Hee Kim ),( 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1

Aims: Hepatitis B virus (HBV) infection leads to hepatic and extrahepatic manifestations including chronic kidney disease (CKD). However, the association between HBV and CKD is not clear. This study aimed to investigate the association between CKD and HBV infection in a population-based, nationwide multicenter study. Methods: A total of 268,422 subjects who underwent health-check examination in nationwide 33 hospitals from Jan 2015 to Dec 2015, were enrolled. We identified 10,473 adults who had chronic HBV infection by hepatitis B surface antigen (HBsAg) positivity (cases), and 41,892 age-and sex-matched HBsAg negative people (controls) in the same dataset. CKD is defined as GFR<60ml/min/1.73² (GFR categories G3a-G5), according to KDIGO 2010 clinical practice guideline. Proteinuria was defined as the presence of urine protein of at least grade 2+. Results: HBsAg positive cases showed significantly higher frequency of CKD (3.3%) than in controls (2.7%) (P=0.006). Also, the prevalence of proteinuria in HBV cases (18.4%) was significantly higher than in controls (13.9%) (P<0.001). Multivariable logistic regression analysis for associated factors of CKD revealed that age, serum protein, serum albumin, hemoglobin, hemoglobinA1c and HBsAg positivity were independent predictors (P<0.05). Also, age, sex, HBsAg positivity, serum protein, hemoglobin, hemoglobinA1c and platelet were independent predictors of the proteinuria (P <0.05). Conclusions: HBV infection was significantly associated with GFR<60 ml/min/1.73² and proteinuria (≥2+). Therefore, in the era of effective antiviral therapy, clinical concern on the extrahepatic manifestations including kidney disease is warranted.

Plenary Session 1 : PS-1-5 ; Is HBsAg seroclearance following nucleoside analogue therapy durable in patients with chronic hepatitis B?

( Gi Ae Kim ),( Young Suk Lim ),( Ju Hyun Shim ),( Kang Mo Kim ),( Han Chu Lee ),( Yung Sang Lee ),( Young Hwa Chung ),( Dan Bi Lee ),( Jih Yun An ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.-

Background: Spontaneous or interferon-induced HBsAg seroclearance is durable in most patients with chronic hepatitis B (CHB). However, little is known about the durability of HBsAg seroclearance following nucleoside analogue (NUC) therapy. Methods: Among 4,578 patients who were treated with either lamivudine (n=1,924) or entecavir (n=2,654) at a tertiary referral hospital in Korea between 2000 and 2010, 121 achieved HBsAg seroclearance. Fifty-eight patients were included in this study after exclusion of 63 patients; acute hepatitis B (n=19), hepatocellular carcinoma (n=8), prior treatment with interferon (n=6), prior immunosuppressive therapy (n=17), liver transplantation (n=6), follow-up loss immediately after HBsAg seroclearance (n=5), and continued NUC therapy (n=2). Results: Mean age of 58 patients were 42 (SD 11) years and 41 (71%) were males. All were assumed to have HBV genotype C. At the initiation of NUC therapy, median levels of ALT and HBV DNA were 153 IU/L (interquartile range [IQR], 48-340 IU/L) and 7.0 log10 copies/mL (IQR, 3.8-8.0 log10 copies/mL), respectively. Twenty-seven (47%) had HBeAg. The median duration of NUC therapy (56 with lamivudine and 2 with entecavir) before HBsAg seroclearance was 42 months (IQR, 24-66 months). During a median follow-up period of 20 months (IQR, 12-32 months), HBsAg reversion occurred in 5 of 58 (8.6%) patients. Three of those 5 patients achieved re-clearance of HBsAg without treatment during further follow-up. The other 2 patients remained HBsAg-positive, but with low titer (<1.0 IU/mL) and undetectable HBV DNA by PCR. Virologic recurrence (detectable HBV DNA by PCR) occurred in 12 of 58 (20.7%) patients. However, all of these patients maintained HBV DNA <10,000 copies/mL. No patient experienced biochemical relapse (ALT flare > x5 ULN). Conclusion: HBsAg seroclearance following NUC therapy is rare but durable in most patients with CHB after treatment discontinuation. Therefore, HBsAg seroclearance would be an ideal treatment endpoint during NUC therapy.

Plenary Session 1 : PS-1-5 ; Is HBsAg seroclearance following nucleoside analogue therapy durable in patients with chronic hepatitis B?

( Gi Ae Kim ),( Young Suk Lim ),( Ju Hyun Shim ),( Kang Mo Kim ),( Han Chu Lee ),( Yung Sang Lee ),( Young Hwa Chung ),( Dan Bi Lee ),( Ji Hyun An ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.1

Background: Spontaneous or interferon-induced HBsAg seroclearance is durable in most patients with chronic hepatitis B (CHB). However, little is known about the durability of HBsAg seroclearance following nucleoside analogue (NUC) therapy. Methods: Among 4,578 patients who were treated with either lamivudine (n=1,924) or entecavir (n=2,654) at a tertiary referral hospital in Korea between 2000 and 2010, 121 achieved HBsAg seroclearance. Fifty-eight patients were included in this study after exclusion of 63 patients; acute hepatitis B (n=19), hepatocellular carcinoma (n=8), prior treatment with interferon (n=6), prior immunosuppressive therapy (n=17), liver transplantation (n=6), follow-up loss immediately after HBsAg seroclearance (n=5), and continued NUC therapy (n=2). Results: Mean age of 58 patients were 42 (SD 11) years and 41 (71%) were males. All were assumed to have HBV genotype C. At the initiation of NUC therapy, median levels of ALT and HBV DNA were 153 IU/L (interquartile range [IQR], 48-340 IU/L) and 7.0 log10 copies/mL (IQR, 3.8-8.0 log10 copies/mL), respectively. Twenty-seven (47%) had HBeAg. The median duration of NUC therapy (56 with lamivudine and 2 with entecavir) before HBsAg seroclearance was 42 months (IQR, 24-66 months). During a median follow-up period of 20 months (IQR, 12-32 months), HBsAg reversion occurred in 5 of 58 (8.6%) patients. Three of those 5 patients achieved re-clearance of HBsAg without treatment during further follow-up. The other 2 patients remained HBsAg-positive, but with low titer (< 1.0 IU/mL) and undetectable HBV DNA by PCR. Virologic recurrence (detectable HBV DNA by PCR) occurred in 12 of 58 (20.7%) patients. However, all of these patients maintained HBV DNA <10,000 copies/mL. No patient experienced biochemical relapse (ALT flare > x5 ULN). Conclusion: HBsAg seroclearance following NUC therapy is rare but durable in most patients with CHB after treatment discontinuation. Therefore, HBsAg seroclearance would be an ideal treatment endpoint during NUC therapy.

Sunggaltang(升葛湯) suppresses LPS-induced NO production and iNOS expression in microglial cells

Gi Hyo Lyu,Seung Yeon Lee,Young Kyun Kim,Young Hee Kim,Kyung Hwa Kang,Hwa Jung Yoon,Hoon Kim,Sun Ae Ryu,Dong IL Park,Woo Shin Ko 한국생명과학회 2006 한국생명과학회 심포지움 Vol.47 No.-