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환자 피부 비늘 및 임상주에서 이중 중합효소 연쇄반응(Nested PCR)을 이용한 Malassezia 균종의 동정
임세웅 ( Se Woong Lim ),신명근 ( Myung Geun Shin ),임주영 ( Ju Young Lim ),윤숙정 ( Sook Jung Yun ),김성진 ( Seong Jin Kim ),이승철 ( Seung Chul Lee ),원영호 ( Young Ho Won ),이지범 ( Jee Bum Lee ) 대한피부과학회 2008 대한피부과학회지 Vol.46 No.4
Background: Due to recent identification of new Malassezia (M.) species, M. dermatis, and M. equi, the genus Malassezia was revised into eleven species that have been isolated from human and animal skin. This has further substantiated the need for molecular techniques to distinguish the various Malassezia species. Objective: We aimed to make the nested polymerase chain reaction (PCR) using species-specific primers with specificity and sensitivity as a diagnostic tool for differentiating the various Malassezia species from skin scales and fungal cells rapidly and accurately. In addition, we evaluated the common causative Malassezia species in the patients with seborreheic dermatitis, pityriasis versicolor or pityrisporum folliculitis. Methods: Malassezia species-specific primers were designed based on DNA sequencing of the ribosomal RNA gene. The standard strains of eight members of the genus Malassezia such M. pachydermatis, M. furfur, M. sympodialis, M. globosa, M. obtuse, M. restricta, M. slooffiae, and M. dermatis were used for positive control. Each Malassezia species was cultured separately and two or three standard species were cultured together on Modified Leeming and Notman agar (MLNA) media plates. In addition, twenty-five clinical strains of Malassezia species isolated from the skin of patients with dermatological conditions and twenty-three samples of skin scale were used as well. Results: The nested PCR assay with primers for all eight Malassezia species were species-specific since it amplified DNA only from the target Malassezia species, and could differentiate mixed, that is, the two or three Malassezia species of all standard strains grown on MLNA medium precisely. Detection of Malassezia species from clinical strains and patient skin scales using the nested PCR assay was 96% (24/25) and 87% (20/23), respectively. M. globosa, M. sympodialis, M. restricta were the most common causative Malassezia species in patients with pityriasis versicolor, pityrosporum folliculitis, seborrheic dermatitis, respectively. Conclusion: Nested PCR using species-specific primers is useful and reliable in the detection of various Malassezia species from patient skin scales as well as cultured fungal cells. (Korean J Dermatol 2008;46(4):446~452)
간 유입혈류 차단 후 간 절제술시 간 저온 및 문맥압 감압이 혈역학적 변화에 미치는 영향
임동건,김병권,김준우,박진웅,이근보,황윤진 대한마취과학회 1999 Korean Journal of Anesthesiology Vol.36 No.6
Background : Portal triad clamping (PTC) during hepatic resection (Pringle maneuver, PM) can afford reduced intraoperative bleeding and bloodless surgical field. But inflow obstruction of blood to liver during PM can bring hemodynamic changes to the patient. This study was designed to evaluate the hemodynamic changes before, during and after PM application during hepatic resection. We also compared the hemodynamic effects of hepatic cooling before PM with/without portal decompression during PM. Methods : The patients were divided into three groups; PM (P group, n=9), PM after hepatic cooling with 400 ml of 4oC lactated Ringer's solution (LR) (C+P group, n=13), PM after hepatic cooling and portal decompression with portocaval shunt (C+P+S group, n=7). Systemic vascular resistance index (SVRI), cardiac index (CI) and mean arterial pressure (MAP) were measured before, during and after PM. Results : Portal pressure of C+P+S group (208.3±36.6 mmH2O) was lower than P (487.3±92.9 mmH2O) and C P (553.6±77.0 moup during PM. CIs of P and C P group were decreased (15, 13% respectively) during PM. After reperfusion, CIs and SVRIs of P, C+P and C+P+S group were all increased (CI; 33, 26, 50%, SVRI; 30, 40, 50%, respectively) than end of PM. Conclusion : PM itself doesn't make abrupt hemodynamic change. Hepatic cooling with 4oC LR (400 ml) before PM increases MAP because of increased SVRI. Reperfusion after PM for 50 minutes, hemodynamic depression could occur by decreased SVRI, especially in case of decompressed portal pressure with portocaval shunt during PM. (Korean J Anesthesiol 1999; 36: 990∼997)
Effect of Seeding Rate on the Yield and Quality of Whole Crop Rice Variety ''Namil"
Jong Geun Kim,Eui Soo Chung,Sei Hyung Yoon,Min Woong Jeong,Byoung Soo Jeon,Nam Chul Cho,Young Chul Lim 한국초지조사료학회 2009 한국초지조사료학회 학술대회논문집 Vol.2009 No.08
This experiment was conducted to evaluate the effect of seeding rate on the quality and yield of whole crop rice variety, "Namil". Whole crop rice variety, "Namil", was direct seeded at 25 April. The seeding rate were four level(30, 60, 90, and 120㎏/㏊). There was not found significantly difference plant height and dry matter(DM) content among seeding rate. Acid detergent fiber(ADF) and neutral detergent fiber(NDF) content was increased until 90㎏/㏊ of seeding rate. The content of TDN(total digestible nutrient) decreased also with increased seeding rate until 90㎏/㏊ seeding rate. The highest fresh and DM yield showed at 120㎏/㏊ seeding rate. But there was not found significantly difference among 60, 90 and 120㎏/㏊ seeding rate. Although high seeding rate increase the DM yield, 60㎏/㏊ seeding rate will be recommendable as proper seeding rate for whole crop rice.
이동통신 네트워크 적용을 위한 Common Public Radio Interface 신호 압축 최적화 방안에 관한 연구
채웅식(Woong Sik CHAE),임종영(Jong Young Lim),유선근(Sun Geun You),장현수(Hyun Su Jang),심재남(Jae Nam Sim),김동구(Dong Ku KIM) 한국통신학회 2016 한국통신학회 학술대회논문집 Vol.2016 No.6
본 논문은 이동통신 네트워크 적용을 위한 CPRI 신호 압축 성능 최적화 방안에 관한 것으로 기술 방식별 CPRI 압축 알고리즘을 분석하고, 압축 파라미터 변경을 통해 압축률을 다양하게 조정함으로써 각각의 조건에서 신호 열화와 신호지연 수준을 분석용 S/W 코드를 제작하여 이론적으로 계산하였으며, 이동통신 네트워크의 특성을 고려한 최적의 적용 조건을 선정하였다. 또한 선정된 압축 조건을 적용한 CPRI 압축 실험용 모듈을 제작하여 이동통신 LTE 랩 환경 및 상용 서비스 네트워크에 적용하여 실험한 결과 50% 압축(2:1)에서 0.2% 이하의 EVM 열화 및 5μsec 수준의 신호지연이 발생하지만 이는 3GPP 의 규격을 만족하는 수준이며 데이터 속도, 접속 성공률, 호단절 발생률 등 서비스 품질에 영향이 없음을 실험적으로 확인하였다.
경운방법과 가축분뇨 시용이 옥수수의 생산성 및 질소의 용탈에 미치는 영향
정민웅(Min-Woong Jung),조남철(Nam-Chul Jo),김종근(Jong-Geun Kim),임영철(Young-Chul Lim),최기춘(Ki-Choon Choi),윤세형(Sei-Hyung Yoon),이기원(Ki-Won Lee),육완방(Wan-Bang Yook) 韓國草地學會 2009 한국초지조사료학회지 Vol.29 No.3
본 연구는 옥수수 재배지에서 경운방법과 가축분뇨의 시용이 사료용 옥수수의 생산성 및 질소의 용탈에 미치는 영향을 알아보기 위하여 국립축산과학원 초지사료과 시험포장 (천안)에서 lysimeter를 이용하여 2008년 4월부터 8월까지 실시하였다. 시험구 배치는 분할구배치법으로 경운방법 (경운과 무경운)을 주구로 하였으며, 가축분뇨의 종류(화학비료, 우분퇴비 및 돈분퇴비)를 세구로 하였다. 가축분뇨의 시용은 화학비료구는 표준시용량 (N-P₂O?-K₂O)으로 200, 150 및 150㎏/ha을 시용하였으며, 우분퇴비와 돈분퇴비의 경우 화학비료구의 질소대비 100%를 전량 기비로 시비하였다. 경운방법에 따른 옥수수의 건물생산성과 뿌리의 무게를 측정한 결과 경운 처리구에서 무경운 처리구보다 높게 나타났으나 유의적인 차이는 없었다. 가축분뇨 시용에 따른 옥수수의 건물생산성은 화학비료 > 우분퇴비 > 돈분퇴비 순으로 유의적으로 높게 나타났으며 (p<0.05), 뿌리 무게는 화학비료구와 우분퇴비구에서 돈분퇴비구 보다 유의적으로 높게 나타났다 (p<0.05). 가축분뇨 시용에 따른 경직경, 당도는 화학비료구와 우분퇴비구에서 돈분퇴비구 보다 유의적으로 높았다 (p<0.05). 옥수수의 생산성과 생육특성에 있어 경운방법과 가축분뇨 시용에 따른 유의적인 교호작용은 나타나지 않았다. 경운방법에 따른 평균 NO₃-N의 용탈은 경운 처리구에서 무경운 처리구보다 약간 높았으나, 통계적인 유의차는 없었다. 하지만 가축분뇨 시용에 따른 NO₃-N의 용탈은 화학비료구에 비해 유의적으로 낮게 나타났다(p<0.05). 본 시험의 결과 사료작물을 재배에서 경운방법이 옥수수의 생산성 및 NO₃-N 용탈에 미치는 영향은 크지 않았으며, 화학비료의 질소대비 100%의 가축분뇨를 시비하였을 경우 용탈되는 NO₃-N의 농도가 높지 않아 수질에 미치는 영향이 미미하였다. Approximately 43 million tons of livestock manure (LM) are produced each year on Korean farms. LM can be utilized as a valuable resource and/or it can contaminate water by runoff and leaching through the soil, when LM has been thoughtlessly applied to the land and directly discharged into the water. This experiment was carried out to investigate the effect of no-till system and LM application on dry matter (DM) yield of silage corn and NO₃-N concentration in leaching water of lysimeter installed in the experimental field. The treatments were replicated three times in split plot design. Main plots consisted of tillage systems, such as conventional tillage (CT) and no-tillage (NT). Sub plots consisted of the type of LM, such as chemical fertilizer (CF), composted cattle manure (CCM) and composted swine manure (CSM). The control plots were fertilized as commercial chemical fertilizer. DM yields of corn increased significantly in order to CF > CCM > CSM (p<0.05). DM yield of corn in CT increased as comparing with that of corn in NT. Plant height, ear height and stem diameter also increased in order to CF > CCM > CSM. In addition, the root weight in CT was increased as comparing with that of corn in NT. However, there was no interaction effects of between type of LM and tillage system. NO₃-N concentration in leaching water of LM application was less than 10 ppm, but NO₃-N concentration in CF exceeded 10 ppm which is safety level of drinking water during summer time (rainfall season).
지형 충돌 회피 및 기동성을 고려한 무인기 경로점 고도계획
임인근(In-Geun Lim),나성웅(Sung-Woong Ra) 한국정보기술학회 2018 한국정보기술학회논문지 Vol.16 No.9
We propose a method to plan the waypoints altitude so that the unmanned aerial vehicle can fly without collision with the terrain, fly at a given altitude in a specific mission performance section, and fly as close to the terrain as possible in the case of no altitude condition. Conventionally, the planner mainly used the method of manually designating the altitude of each of the waypoints, but it has a disadvantage that the results are different each time and it takes a lot of time. Recently, an automatic altitude planning method has been proposed, but it is a planning to fly a long distance at a high altitude to prevent terrain collision. Therefore, it is not suitable for performing missions where low altitude flight is required. We check the possibility of terrain collision by comparing the terrain elevation with the expected flight altitude in a segmentation. If there is a risk of terrain collision, adjust the waypoint altitude value in different ways according to the seven cases to raise the altitude only for the minium interval. The range of elevation or descent altitude was calculated by the distance of the front and rear waypoints. The waypoint altitude is finally determined by taking into account the altitude of the terrain as well as the maneuverability of the UAV.
급성 골수성 백혈병 환자에서 발생한Fusarium solani에 의한 전신 감염증
임세웅 ( Se Woong Lim ),김형준 ( Hyung Joon Kim ),신명근 ( Myung Geun Shin ),이재혁 ( Jae Hyuk Lee ),윤숙정 ( Sook Jung Yun ),이지범 ( Jee Bum Lee ),김성진 ( Seong Jin Kim ),원영호 ( Young Ho Won ),이승철 ( Seung Chul Lee ) 대한피부과학회 2008 대한피부과학회지 Vol.46 No.6
Fusarium species are known as ubiquitous soil saprophytes and human skin contaminants. Infections caused by Fusarium species are increasing in frequency among immunocompromised hosts. We report a rare case of fatal disseminated infection caused by Fusarium solani in an acute myeloid leukemia patient. Skin biopsy specimen revealed multiple branched hyphae and spores in the vessel with thrombus formation, and fungal culture showed a fuzzy, whitish colony. Direct sequencing of 28S rRNA gene confirmed the isolate as Fusarium solani. (Korean J Dermatol 2008;46(6):846∼850)