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Anodonta arcaeformis와 Cristaria plicata 유생의 미세구조적 연구
박갑만 關東大學校 醫科大學 醫科學硏究所 2002 關東醫大學術誌 Vol.6 No.1
Two species of freshwater mussels in Korean, Anodonta araceformis and Cristaria plicata, were compared in regard to the vaious texononmical characteristics of glochildian. The out-demibranchs of all females of species have served as marsupial pouch. The breeding season of these two species was the winter breeder. The glochidia of A. arcaeformis and C. plicata were separated one by with the mucous strings. The shape of the glochidium of A. arcaeformis and C. plicata is apparently sub-triangular and is average size is 250 μm in 240 μ in height and 230×310 μm, respectively. Two glochidial shell valves are of same size, kept together by ligament of 235 μm and 220 μm in width. Each of the glochidial shell valves has studded with many spines on the superior face. a large area at the apex of the valve surrounding the base of the hook is provided with numerous small spines which become progressively smaller towards the periphery of the area. A larval thread of A. arcaeformis and C. plicata, without any accessory structure and 2.6 μm and 2.4 μm in diameter, emerges from a canal located at center of ventral plate of the mantle. The results indicated that the glochidial morphology be reveal characters useful for the classification of different species and for the interrelations between genera.
권오길,박갑만,이준상,송호복 江原大學校 附設 環境硏究所 1991 環境硏究 Vol.8 No.-
This study was carried out to determine the attachment parts and rates on the host fish and the successive stages of the glochidial encystment. A scanning electron microscope observation of the glochidia, the larvae of freshwater brivalve, Anodonta arcaeformis flavotincta, on the host fish, Carassius auratus, was conducted. The attachment rates on the host fish were 30.1%, 22.3% on the pectoral, the caudal, and the pelvic and anal fin, respectively. Glochidia, once attached to the host, have become encysted within 27 hours. The glochidia in fish tissues have sloughed between 13 days and 15 days when the water temperature was 16℃ in laboratory. During encystment, the epithelial cells of the host fish migrated actively toward the attached glochidia and covered them. In the encystment process, the encysted tissues in the host have become loose in seven days and exposured a pat of the glochidium in eight days after infection. No changes of the glochidial size have found before attachment and after sloughing.
Park, Gab-Man,Chung, Ee-Yung,Hur, Sung-Bum 한국유전학회 2002 Genes & Genomics Vol.24 No.1
Geographical isolates of the shortnecked clam Ruditapes philippinarum in Korea were examined at the molecular level for genetic divergence. Sequences from both nuclear and mitochondrial genomes were selected as markers of evolutionary divergence. Sequence analysis of the second internal transcribed spacer region (ITS2) within the rDNA repeat and the mitochondrial cytochrome c oxidase I (mtCOI) gene were performed. Very slight variation of intra-species in R. philippinarum was detected from the mtCOI sequence (2 bp) and ITS2 sequence (4 bp). The mtCOI and ITS2 also exhibited no intra-individual variation from color patterns. The three populations of R. philippinarum employed in this study showed very slight variation in the nucleotide sequences of the mtCOI and ITS2 genes. Phylogenetic analysis of mtCOI sequence data confirmed R. philippinarum to be a member of the Veneroida.
긴 애기물달팽이 (복족강 : 물달팽이과)의 염색체에 관한 연구
Park, Gab-Man,Kim, Jae-Jin 한국패류학회 1996 The Korean Journal of Malacology Vol.12 No.2
1996년 8월 춘천 의암호에서 채집한 긴애기물달팽이(Fossaria truncatula)를 재료로 공기건조법을 이용하여 통상적인 방법으로 염색체를 관찰하고 핵형을 분석하였다. 그 결과 긴애기물달팽이의 염색체 수는 2n=31, 32이었으며 15쌍의 배수체와 1개의 성염색체를 가지고 있었다. 핵형은 3쌍의 metacentric과 13쌍의 submetacentric 염색체로 구성되어 있었다.
Karyotypes of Korean Endemic Land Snail, Koreanohadra koreana (Gastropoda: Bradybaenidae)
Park, Gab-Man The Malacological Society of Korea 2011 The Korean Journal of Malacology Vol.27 No.2
The karyotypes of Korean endemic land snail, Koreanohadra koreana, using air-drying method wereinvestigated. Somatic cells of this species had 2n = 58. Karyotypes were also analysed with 16 metacentric, 12 submetacentric and one subtelocentric chromosome pairs. Observed chromosomes ranged from 2.6 to $8.9{\mu}m$ and the total length was $122.3{\mu}m$. This is the second report on the chromosome numbers and the karyotype of K. koreana.
Genetic Characterization based on Partial 28S rRNA Gene Sequence of Korean Two Scallops
Park, Gab-Man 한국패류학회 1997 The Korean Journal of Malacology Vol.13 No.1
한국산 가리비, 큰가리비(Patinopecten yessoensis)와 주문진가리비(Chlamys swifti), 2종에 대한 28S ribosomal RNA 유전자의 PCR- 산물을 이용 RFLP 및 염기서열을 밝히고, 이미 보고된 2과 3종의 염기서열과 상동성을 비교 분석하였다. 그 결과 28S rRNA유전자를 이용하여 7가지 제한효소를 처리한 PCR-RFLP의 종간 차이에서 Taq I 제한효소에서만 차이를 볼 수 있었다. 한편 두종간에 28S rRNA유전자의 D1 부위의 염기서열에서 231개 부위 중 14군데에서 변이를 보였다.
Park, Gab-Man,Shin, Ho-Sung JOURNAL OF PARASITOLOGY 2016 The Journal of parasitology Vol.102 No.2
<P>The geographical distributions and relative population densities of scrub typhus vector mites collected from small mammals were determined for 5 locations on the east coast of the Republic of Korea. Collection sites included Goseong, Gangneung, and Hoengseong in Gangwon province and Uljin and Yeongdeok in Gyeongbuk province. A total of 275 small mammals including members of Rodentia (rodents) and Soricomorpha (shrews, such as Crocidura lasiura) belonging to 4 genera and 4 species were captured in the field from 2012 to 2013. Apodemus agrarius was collected most frequently (220, 80%), followed by C. lasiura (25, 9.1%), Mus musculus (15, 5.5%), and Myodes regulus (15, 5.5%). A total of 23,436 larval chigger mites (Family Trombiculidae) belonging to 3 genera and 8 species (Leptotrombidium pallidum, Leptotrombidium scutellare, Leptotrombidium palpale, Leptotrombidium orientale, Leptotrombidium zetum, Neotrombicula tamiyai, Neotrombicula japonica, and Euschoengastica koreaensis) were collected from the small mammals. The predominant chigger species collected during the spring and fall seasons from A. agrarius were L. pallidum (57.6%), L. palpale (14.5%), and L. scutellare (7.9%). Leptotrombidium scutellare was collected only along the southeastern coast at Yeongdeok, Gyeongbuk province. The geographical distribution of scrub typhus vectors and reservoir hosts are important aspects of understanding the epidemiology of the disease as well as the potential impacts of climate change and health risks.</P>