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Genetic Analysis and Event-Specific Detection of a CGMMV-CP Transgenic Watermelon Rootstock Line
Eun Soo Youk,In Soon Pack,Yu-Jin Kim,Won Kee Yoon,Chang-Gi Kim,Stephen B. Ryu,Chee Hark Han,Soon-Chun Jeong,Hwan Mook Kim 한국작물학회 2008 한국작물학회 학술발표대회 논문집 Vol.2008 No.10
Transgenic plants that over express virus coat protein genes have attracted particular interest from researchers, by virtue of their tolerance to virus infection. The transgenic watermelon rootstock analyzed in this study was established by introducing CGMMV coat protein (cp) under the control of CaMV 35S promoter and NOS terminator (Park et al., (2005) Plant Cell Rep. 24: 350-6). The primary objective of this study was to determine the copy number and integration site of the transgene element, in order to develop detection techniques required for monitoring of the transgenic watermelon rootstock. The Southern blot analysis indicated that a single copy of CGMMV-cp gene was inserted into the genome of transgenic watermelon rootstock. We also identified the genomic sequences flanking the integration site of the transgene by inverse PCR analysis. In an effort to find a sequence usable as an internal positive control for the screening of the watermelon and watermelon rootstock, we found that the Sat and DIP-1 genes appears as one copy within their genomes and is watermelon rootstock- and watermelon-specific. The information of the integrated site and the internal positive control sequence was used to establish a new event-specific PCR-based detection method. In addition, mRNA and protein expression level of the transgene in the transgenic watermelon rootstock and grafted watermelon were investigated. The expression of both mRNA and protein of CGMMV-CP was not detected in the transgenic watermelon rootstocks and watermelons, suggesting that the movement of transgene products from transgenic rootstock to watermelon does not occur at our detection level.
Youk, Jin-Soo,Kim, Young-Hee,Kim, Eun-Jin,Youn, Na-Jin,Chang, Suk-Kyu Korean Chemical Society 2004 Bulletin of the Korean Chemical Society Vol.25 No.6
Newly synthesized 8-hydroxyquinoline based benzothiazole derivative 2 showed a distinctive $Hg^{2+}$-selectivity over other transition metal ions in aqueous solution. The fluorescence emission at 455 nm of 2 was completely quenched upon interaction with $Hg^{2+}$ ions in dioxane-$H_2O$ system (9 : 1, v/v). The selectivity was decreased in the order of $Hg^{2+}\;>>\;Cu^{2+}\;>\;Cd^{2+}\;>\;Pb^{2+}\;{\thickapprox}\;Zn^{2+}\;{\thickapprox}\;Ni^{2+},\;and\;Hg^{2+}$ concentration dependent fluorescence quenching profile was observed in the presence of common interfering metal ions as background. The fluorescence behavior of 2 suggests that the prepared compound could be used as a fluorescent signaling subunit for the construction of new $Hg^{2+}$-sensitive ON-OFF type supramolecular switching systems.
Jeong, Soon-Chun,Pack, In Soon,Cho, Eun-Young,Youk, Eun Soo,Park, Sangkyu,Yoon, Won Kee,Kim, Chang-Gi,Choi, Yang Do,Kim, Ju-Kon,Kim, Hwan Mook Elsevier 2007 FOOD CONTROL Vol.18 No.11
<P><B>Abstract</B></P><P>The remarkable amount of public and scientific debate with regard to the safety of genetically modified (GM) crops has resulted in the implementation of mandatory risk assessments of newly developed GM crops, as well as mandatory labeling of GM crops and foods following their commercial release. Among the many currently available GM rice lines, transgenic rice lines that overexpress a trehalose-6-phosphate synthase/phosphatase (<I>TPSP</I>) fusion gene have attracted particular interest from researchers, by virtue of their superior tolerance to a variety of abiotic stresses. The primary objective of this study was to determine the copy numbers of the transgene element, and to identify the genomic sequences flanking the integration site of the transgene element in a selected <I>TPSP</I> transgenic rice line, in order to develop new event-specific detection techniques. The genomic sequences flanking the integration site of the transgene element were identified via an inverse PCR protocol. It was determined that a single copy of the <I>TPSP</I> fusion had been integrated into the transgenic line. An effort to find a rice sequence usable as an internal positive control for the screening of the transgenic rice resulted in the identification of an <I>RBE4</I> gene sequence, which appears as one copy within the rice genome, and is rice-specific. The characterization of the genomic sequences flanking the transgene, as well as the availability of the internal positive control sequence, enabled the design of a qualitative PCR technique and a quantitative TaqMan-based detection method for this transgenic rice line. The results of this study may prove useful with regard to the large-scale screening of this transgenic rice line in the processes of risk assessment and commercialization, as well as in the development of novel methods for the detection of other transgenic rice lines.</P>
A Molecular Framework for Risk Assessment of a Virus-Tolerant Transgenic Pepper Line
Pack, In-Soon,Kim, Yu-Jin,Youk, Eun-Soo,Lee, Woo-Kyu,Yoon, Won-Kee,Park, Kee-Woong,Kim, Chang-Gi,Harn, Chee-Hark,Kim, Hwan-Mook,Jeong, Soon-Chun 한국작물학회 2012 Journal of crop science and biotechnology Vol.15 No.2
The Cucumber mosaic virus coat protein (CMV-CP) gene-transgenic pepper lines exhibit high tolerance to Cucumber mosaic virus (CMV) strains. In this study, E7, one of the CMVP0-CP transgenic chili pepper events selected by screening was further characterized. Southern blotting and inverse PCR analysis revealed that the E7 event contains a single copy of the inserted gene cassette whose flanking sequences appear to be noncoding and intergenic. We searched for pepper-specific DNA sequence candidates as an endogenous reference gene for GM-pepper detection. We found that only one copy of CaSIG4 and lipocalin genes are present in the pepper genome and their sequences were determined to be pepper-specific. The characterization of the genomic sequences flanking the transgene, as well as the availability of the pepper-specific single copy CaSIG4 and lipocalin genes as endogenous reference genes, enabled the design of E7-event-specific PCR-based quantitative detection methods. The CMV-CP protein levels in the CMV-inoculated wild-type pepper tissues were approximately 60 times higher than those in the uninoculated and CMV-inoculated E7 pepper tissues. These results suggested that the amount of CMV-CP expressed in transgenic pepper tissue was negligible relative to the amount of CMV-CP in the virus-infected wild-type pepper consumed by human beings. This work may prove useful for risk assessment studies of transgenic pepper lines. Furthermore, the characterized single copy genes, lipocalin and CaSIG4, may be used to develop a method to detect gene copy number variations in the pepper genome.
서동현 ( Dong-hyun Seo ),이상호 ( Sang-ho Lee ),육은비 ( Eun-bi Youk ),박태영 ( Tae-yeong Park ),이혜원 ( Hye-won Lee ),김인수 ( In-soo Kim ) 한국정보처리학회 2022 한국정보처리학회 학술대회논문집 Vol.29 No.2
본 논문에서는 현대인들의 일상생활 속 누적된 스트레스를 완화하고 사용자의 편의를 고려한 “아로마 테라피를 지원하는 지능형 샤워부스” 시스템을 제안한다. 제안하는 시스템의 주요 기능은 다음과 같다. 첫째, 적외선 온도 센서와 초음파 센서, 카메라를 통해 사용자의 신체 정보와 기분을 측정한다. 둘째, 측정된 사용자의 신체 정보를 반영하여 Linear actuator를 이용해 샤워기의 높낮이 및 수온을 자동으로 조절한다. 셋째, OpenCV와 앱 내에 만족도 평가를 통해 사용자의 기분에 따라 알맞은 아로마오일을 추천하고 이를 샤워기 필터에 주입한다. IoT기술과 연동된 샤워부스 시스템을 통해 사용자 컨디션에 맞춘 아로마테라피를 지원하여 현대인의 지친 심신 회복과 사용자 편의성이 증대될 것으로 기대된다.