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Antioxidative Constituents from Lycopus lucidus
Woo, Eun-Rhan,Piao, Mei-Shan The Pharmaceutical Society of Korea 2004 Archives of Pharmacal Research Vol.27 No.2
Three phenolic compounds, rosmarinic acid (1), methyl rosmarinate (2), ethyl rosmarinate (3), and two flavonoids, luteolin (4), luteolin-7-O-$\beta$-D-glucuronide methyl ester (5) were isolated from the aerial part of Lycopus lucidus (Labiatae). Their structures were determined by chemical and spectral analysis. Compounds 1-5 exhibited potent antioxidative activity on the NBT superoxide scavenging assay. The $IC_{50}$ values for compounds 1-5 were 2.59, 1.42, 0.78, 2.83, and 3.05 $\mu\textrm{g}$/mL respectively. In addition, five compounds were isolated from this plant for the first time.
Anti-herpetic Activity of Various Medicinal Plant Extracts
Woo, Eun-Rhan,Kim, Hyoung-Ja,Jong, Hwan-Kwak,Lim, Young-Kwern,Park, Seong-Kyu,Kim, Hae-Soo,Lee, Chong-Kyo,Park, Hokoon The Pharmaceutical Society of Korea 1997 Archives of Pharmacal Research Vol.20 No.1
In order to find antiviral compounds against Herpes simplex virus type I (HSV-1) and II (HSV2) from natural products, a convenient virus-induced cytopathic effect (CPE) inhibition assay was introduced. More than 300 fractions were prepared by solvent fractionation from sixty collected plants or purchased herbal medicines, and their anti-herpetic activities were evaluated. Among them, several medicinal plants showed potent anti-herpetic activity. Selective indexes (SI) of the EtOAc extract of Caraganae Radix (Caragana sinica) against HSV-1 and HSV-2 were more than 8.06 and 24.79, SI of the MeOH extract of Acer okamotoanum leaves were 3.92 and 3.51, SI of the $CH_{2}Cl_{2}$ extract of Veratri Rhizoma et Radix (Veratrum patulum) were 5.49 and 1.31 and SI of the MeOH extract of aerial part of Osmundae Rhizoma (Osmunda japonica) were more than 3.45 and 1.25, respectively.
Styraxjaponoside A and B, Antifungal Lignan Glycosides Isolated from Styrax japonica S. et Z.
( Eun Rhan Woo ),( Dong Gun Lee ),( Jae Yong Cho ),( Mi Ran Kim ),( Ca Na Park ),( Bo Mi Hwang ),( In Sok Hwang ) 한국응용약물학회 2010 Biomolecules & Therapeutics(구 응용약물학회지) Vol.18 No.4
The antifungal effects and action mechanisms of styraxjaponoside A and B were investigated. Devoid of hemolytic effect, the compounds had significant effect against several human pathogenic fungal strains, with energy-independent manners. To understand the action mechanisms of the compounds, the flow cytometric analysis plotting the forward scatter and the side scatter, DiBAC4(3) staining and DPH fluorescence analysis were conducted. The results indicated that the actions of the compounds were dependent upon the membrane-active mechanisms. The present study suggests that styraxjaponoside A and B exert their antimicrobial effects via membrane-disruptive mechanisms.
Virus-cell Fusion Inhibitory Activity for the Polysaccharides from Various Korean Edible Clams
Woo, Eun-Rhan,Kim, Wan-Seok,Kim, Yeong-Shik The Pharmaceutical Society of Korea 2001 Archives of Pharmacal Research Vol.24 No.6
In order to find potent virus-cell fusion inhibitory components from Korean edible clams, thirteen prepared polysaccharides were introduced to syncytia formation inhibition assay, which is based on the interaction between the HIV-1 envelope protein gp 120/41 and the cellular membutane protein CD4 of T lymphocytes. Among them, Meretrix petechialis showed a potent viruscell fusion inhibitory activity. Fusion index (F1) and percent (%) fusion inhibition of the polysaccharide of this clam were $0.21{\pm}0.02$, and $67.52{\pm}4.09$ at 100781m1, respectively. It exhibited almost equivalent virus-cell fusion inhibitory activity to that of dextran sulfate which was used as a standard control.
Inhibition of Nuclear Factor-κB Activation by 2′,8″-Biapigenin
Woo, Eun-Rhan,Pokharel, Yuba Raj,Yang, Jin Won,Lee, Song Yi,Kang, Keon Wook Pharmaceutical Society of Japan 2006 Biological & pharmaceutical bulletin Vol.29 No.5
<P>Inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) play a key role in the inflammatory processes. Improper overproduction of NO and prostaglandins by both enzymes are also believed to be involved in the pathogenesis of certain human cancers. Crude extracts of <I>Selaginella tamariscina</I> are used as an oriental medicine, which has been reported to inhibit the production of proinflammatory cytokines and cause cell cycle arrest. We isolated 2′,8″-biapigenin from <I>S. tamariscina</I> and investigated whether it modulates iNOS and COX-2 expressions in Raw264.7 macrophages stimulated with lipopolysaccharide (LPS). We found that 2′,8″-biapigenin blocked the transactivations of iNOS and COX-2 genes <I>via</I> the inactivation of nuclear factor-κB by preventing the nuclear translocation of p65. Hence, it may be possible to develop <I>S. tamariscina</I> extracts or 2′,8″-biapigenin as a useful agent for cancer chemoprevention or for the treatment of inflammatory diseases.</P>
마타리 뿌리로부터 분리한 Lignan 화합물의 IL-6 저해활성
최은진(Eun Jin Choi),김청룡(Qinglong Jin),신지은(Ji Eun Shin),김현규(Hyun-Gyu Kim),김정진(Jung Jin Kim),우은란(Eun-Rhan Woo) 대한약학회 2009 약학회지 Vol.53 No.4
In an ongoing investigation into anti-inflammatory compounds from natural products, the CH2Cl2soluble fraction of Patrinia scabiosaefolia F. (Valerianaceae) was found to inhibit IL-6 production in TNF-α stimulated MG-63 cell line. By means of a bioassay-directed chromatographic separation technique, lappaol E (1), and nortrachelogenin (2) were isolated. These compounds have been isolated from this plant for the first time. Compounds 1~2 showed potent antioxidative activity using NBT superoxide scavenging assay. Moreover, compound 1 decreased IL-6 production in TNF-α stimulated MG-63 cell line.
Ji Eun Shin,Eun-Rhan Woo,Eun Jin Choi,Qinglong Jin,Hong-Guang Jin 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.3
Six chalcone compounds, 2',4',4-trihydroxy-3'-[2-hydroxy-7-methyl-3-methylene-6-octaenyl]chalcone (1), 2',4',4-trihydroxy-3'-geranylchalcone (2), 2',4',4-trihydroxy-3'-[6-hydroxy-3,7-dimethyl-2,7-octadienyl]chalcone (3), 2',4-dihydroxy-4'-methoxy-3'-[2-hydroperoxy-3-methyl-3-butenyl]chalcone (4), 2',4-dihydroxy-4'-methoxy-3'-geranylchalcone (5), and 2',4-dihydroxy-4'-methoxy-3'-[3-methyl-3-butenyl]chalcone (6) were isolated from the leaves of Angelica keiskei K (Umbelliferae). The structure of each isolated compound was determined using spectroscopic methods. Among the isolates, compounds 1-3 appeared to have potent inhibitory activity of IL-6production in TNF-α-stimulated MG-63 cell, while compounds 4-6 did not. The distinct structural difference between compounds 1-3 and 4-6 was the presence of C-4' hydroxyl group in the chalcone moiety. Our results imply that the inhibitory activity of IL-6 production in TNF-α-stimulated MG-63 cell may be affected by the presence of C-4' hydroxyl group in the chalcone moiety.
Structural Implication in Cytotoxic Effects of Sterols from Sellaginella tamariscina
Roh, Eun-Mi,Jin, Qing-Long,Jin, Hong-Guang,Shin, Ji-Eun,Choi, Eun-Jin,Moon, Young-Hee,Woo, Eun-Rhan 대한약학회 2010 Archives of Pharmacal Research Vol.33 No.9
A bioassay-guided fractionation of the $CH_2Cl_2$ extract of Selaginella tamariscina yielded six sterols 1-6 such as (4${\alpha}$, 5${\alpha}$)-4, 14-dimethylcholest-8-en-3-one (1), ergosta-4, 6, 8(14), 22-tetraene-3-one (2), ergosterol endoperoxide (3), 7${\beta}$-hydroxycholesterol (4), 7${\beta}$-hydroxysitosterol (5), and 7${\alpha}$-hydroxysitosterol (6). The structures of isolated compounds were determined using spectroscopic methods. Among these isolates, compounds 2-5 showed potent cytotoxicity against five human tumor cells, while compounds 1 and 6 did not. In the case of compounds 1 and 2, 3-oxo sterol derivatives, compound 1 was inactive, but compound 2 showed potent cytotoxicity. In addition, compound 5 exhibited potent cytotxicity, but, compound 6 which is the 7-epimer of compound 5 was weakly active against tumor cell lines. Therefore, in the case of oxysterol derivatives, the cytotoxicity appeared to be affected by the structural differences, i.e. the configuration of hydroxyl group and the number of conjugated double bond. Taken all together, the present study isolated six sterols from S. tamariscina for the first time based on a bioassay-guided fractionation and indicated that isolated oxysterols could exhibit the cytotoxic effects against tumor cells, suggesting that S. tamariscina might be a promising candidate for the development of anticancer agents.