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RISS 인기검색어
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- 저자 : ( Eun Jm Choi ) (검색결과 3 건)
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양은진(Eun-Jm YANG),최송표(Song-Pya CHOI),조근자(Keun-Ja CHO),김수일(Soo-II KIM),권오유(O-Yu KWON),이영호(Young-Ho LEE),김원식(Won-Slk KIM) 대한체질인류학회 1997 대한체질인류학회지 Vol.10 No.2
항문직장암 등의 치료에 입상에서 많이 사용되고 있는 FUDR의 최기효과와 죄기기전의 일부를 알아보기 위하여, 흰쥐(rat, Sprague-Dawley계)를 실험동물로 임신 10 5 일에 FUDR (Sigma사 제품 )60㎎/kg, 65 mg/kg, 70㎎/kg을 각각 단회 복강내 투여하고 임신 17 5 일에 ether마취하에 희생시켜 임신낭의 수, 생존태아의 수, 태아체중 등을 계측하고, 외형상 출연한 기형을 입제현미경으록 관찰하여, 나타난 기형의 종류, 투여량에 따른 각종 기형의 발생 빈도 등을 비교한바 다음과 같은 결과를 얻었다 1.본 실험에서 FUDR의 투여로 유발원 선천성 기형에는 물머리중, 수정체결손 입천장파열, 꼬리기형, 사지기형 등이었으며, 사지기형에는 엄지 및 둘째 발가락결손, 엄지 및 둘째 발가락 발육부전 바다표범발증, 발갈림증 , 흩발가락, 발가락 융합증 등이었다. 2. FUDR로 유발된 선천성기형은 기관의 종류에 따라 또한 투여량의 증가에 따라 발생빈도가 서로 달랐다 3. 사지기형에 있어서는 전 후지간에 그리고 전지의 좌우측간에 그 발생빈도가 현저히 달랐다 이상의 경파로 미루어 볼 때 FUDR은 흰쥐에서 다양한 선천성기형을 유발하고 그들의 발생빈도나 최기효과는 투여량에 따라 현저한 차이를 보임을 알 수 있었으나, 전지에서 좌우측간의 차이 등 보다 명확한 최기기전에 관해서는 향 후 더 연구해야 할 것으로 사료된다.
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Establishment of porcine embryonic stem cells from parthenogenetically activated blastocysts
( Soo Kung Jung ),( Hyun Jung Kim ),( Eun Song Lee ),( Jeong Mook Lim ),( Eun Jm Choi ),( Hee Soo Lee ),( Kyung Bong Koh ),( Jae Young Song ),( Kyung Woo Lee ),( Sang Ho Cha ) 한국예방수의학회(구 한국수의공중보건학회) 2012 예방수의학회지 Vol.36 No.2
Porcine has been known to have a great impact on the studies of organ transplantation, biomaterial production and specific biomodel development such as transgenic animals. To achieve such therapeutic purposes, establishment of porcine embryonic stem cells (pESCs) will be needed. Especially, in vitro differentiation toward neural cells from pESCs can be a useful tool for the study of early neural development and neurodegenerative disorders. In addition, these cells can also be used in cell replacement therapies and drug development for neuroprotective and/or neurotoxic reagents. Although several studies reported the successful isolation of pES-like cells, it has been a big challenge to determine optimal conditions to generate pESCs without loss of pluripotency for a long time. The present study was performed for generation and characterization of putative pESCs, and differentiation into neurons and astrocytes. In this study, porcine blastocysts were produced by parthenogenetically activated oocytes. The putative pESCs were cultured in pESC growth media supplemented with a growth factor and cytokines (bFGF, LIF and SCF). Subculture of pESCs was conducted by mechanical dissociation using syringe needles after 4-5 days of incubation. As results, six putative pESC lines were maintained over thirty passages. The putative pESCs were compact, round, flat, and single layered, which were similar to human embryonic stem cell morphologically. Six pES-like cells were positive for alkaline phosphatase activity at every three passages. Furthermore, Oct-3/4, Sox-2, Nanog and SSEA-4 were shown to be expressed in those cells. Also, normal karyotypes of pESCs were observed by Giemsa-staining. Differentiation potential into the three germ layers of the putative pESCs was demonstrated by the formation of embryoid bodies (EB). Besides, the study of ESC is very important in aspect of its application to not only the cell-based replacement therapies but also cellular differentiation research. Our results also showed that RA and N2 supplements activated the neural differentiation in pESC5. Neurofilament-l60 were expressed in neural precursor cells. The expression of markers for specific neural lineages, such as Microtubule-associated protein-2 expressed in matured neuron, was also induced from embryonic neural progenitors. In summary, the pESCs were generated from the parthenogenetically activated blastocysts and the typical characteristics of the cells were maintained for the long term culture. Furthermore, it was successful to differentiate the pESCs into various neural lineages through in vitro neurogenesis system. Eventually, pESCs will be excellent biomedicine in incurable and/or zoonotic diseases by regenerating the damaged tissue.
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