A Synthetic Naringenin Derivative, 5-Hydroxy-7,4′-diacetyloxyflavanone-<i>N</i>-phenyl Hydrazone (N101-43), Induces Apoptosis through Up-regulation of Fas/FasL Expression and Inhibition of PI3K/Akt Signaling Pathways in Non-Small-Cell Lung Cancer Cells

Bak, Yesol,Kim, Heejong,Kang, Jeong-Woo,Lee, Dong Hun,Kim, Man Sub,Park, Yun Sun,Kim, Jung-Hee,Jung, Kang-Yeoun,Lim, Yoongho,Hong, Jintae,Yoon, Do-Young American Chemical Society 2011 Journal of agricultural and food chemistry Vol.59 No.18

<P>Naringenin, a well-known naturally occurring flavonone, demonstrates cytotoxicity in a variety of human cancer cell lines; its inhibitory effects on tumor growth have spurred interest in its therapeutic application. In this study, naringenin was derivatized to produce more effective small-molecule inhibitors of cancer cell proliferation, and the anticancer effects of its derivative, 5-hydroxy-7,4′-diacetyloxyflavanone-<I>N</I>-phenyl hydrazone (N101-43), in non-small-cell lung cancer (NSCLC) cell lines NCI-H460, A549, and NCI-H1299 were investigated. Naringenin itself possesses no cytotoxicity against lung cancer cells. In contrast, N101-43 inhibits proliferation of both NCI-H460 and A549 cell lines; this capacity is lost in p53-lacking NCI-H1299 cells. N101-43 induces apoptosis via sub-G<SUB>1</SUB> cell-cycle arrest in NCI-H460 and via G<SUB>0</SUB>/G<SUB>1</SUB> arrest in A549 cells. Expression of apoptosis and cell-cycle regulatory factors is altered: Cyclins A and D1 and phospho-pRb are down-regulated, but expression of CDK inhibitors such as p21, p27, and p53 is enhanced by N101-43 treatment; N101-43 also increases expression levels of the extrinsic death receptor Fas and its binding partner FasL. Furthermore, N101-43 treatment diminishes levels of cell survival factors such as PI3K and p-Akt dose-dependently, and N101-43 additionally induces cleavage of the pro-apoptotic factors caspase-3, caspase-8, and poly ADP-ribose polymerase (PARP). Cumulatively, these investigations show that the naringenin derivative N101-43 induces apoptosis via up-regulation of Fas/FasL expression, activation of caspase cascades, and inhibition of PI3K/Akt survival signaling pathways in NCI-H460 and A549 cells. In conclusion, these data indicate that N101-43 may have potential as an anticancer agent in NSCLC.</P>

Sodium butyrate has context-dependent actions on dipeptidyl peptidase-4 and other metabolic parameters

Lee, Eun-Sol,Lee, Dong-Sung,Pandeya, Prakash Raj,Kim, Youn-Chul,Kang, Dae-Gil,Lee, Ho-Sub,Oh, Byung-Chul,Lee, Dae Ho The Korean Society of Pharmacology 2017 The Korean Journal of Physiology & Pharmacology Vol.21 No.5

Sodium butyrate (SB) has various metabolic actions. However, its effect on dipeptidyl peptidase 4 (DPP-4) needs to be studied further. We aimed to evaluate the metabolic actions of SB, considering its physiologically relevant concentration. We evaluated the effect of SB on regulation of DPP-4 and its other metabolic actions, both in vitro (HepG2 cells and mouse mesangial cells) and in vivo (high fat diet [HFD]-induced obese mice). Ten-week HFD-induced obese C57BL/6J mice were subjected to SB treatment by adding SB to HFD which was maintained for an additional 16 weeks. In HepG2 cells, SB suppressed DPP-4 activity and expression at sub-molar concentrations, whereas it increased DPP-4 activity at a concentration of $1,000{\mu}M$. In HFD-induced obese mice, SB decreased blood glucose, serum levels of insulin and $IL-1{\beta}$, and DPP-4 activity, and suppressed the increase in body weight. On the contrary, various tissues including liver, kidney, and peripheral blood cells showed variable responses of DPP-4 to SB. Especially in the kidney, although DPP-4 activity was decreased by SB in HFD-induced obese mice, it caused an increase in mRNA expression of $TNF-{\alpha}$, IL-6, and $IL-1{\beta}$. The pro-inflammatory actions of SB in the kidney of HFD-induced obese mice were recapitulated by cultured mesangial cell experiments, in which SB stimulated the secretion of several cytokines from cells. Our results showed that SB has differential actions according to its treatment dose and the type of cells and tissues. Thus, further studies are required to evaluate its therapeutic relevance in metabolic diseases including diabetes and obesity.

Shelf Life Determination of Precooked Frozen Meat Dumplings

Lee, Young-Chun,Yang, Hyung-Sub,Kim, Dong-Ho 한국산업식품공학회 2002 산업 식품공학 Vol.6 No.3

Quality of frozen foods is significantly affected by the storage temperature and tie, and the consumers' demand for safe quality frozen foods is increasing. The objective of this research was to estimate the shelf life of frozen cooked meat dumplings, based on the changes in the selected physico-chemical and sensory quality attributes. Precooked frozen meat dumplings sampled from the same lot produced at a frozen food company in Seoul were stored at -10, -15, and -20℃. The meat dumplings sampled at 3- to 4-week intervals were subjected to the determination of volatile basic nitrogen (VBN), thiobarbituric acid (TBA), peroxide value (POV), surface color and shear value after thawing at 5℃ for 15 h. High quality life (HQL) was determined through the triangle test and practical quality life (PQL) through the scoring test. VBN, TBA and POV increased as the storage period was increased. HQL of the frozen meat dumplings were 189 and 252 days at -10 and -15℃, respectively. The estimated PQL were 349, 475, and 491 days at -10, -15, and -20 ℃, respectively. Statistical analysis indicated that there were significant relationships between sensory scores and VBN and POV of the stored meat dumplings. VBN was selected as the most reliable objective quality index for the shelf life determination (PQL) of the precooked frozen meat dumplings.

2,4-bis (p-hydroxyphenyl)-2-butenal (HPB242) induces apoptosis via modulating E7 expression and inhibition of PI3K/Akt pathway in SiHa human cervical cancer cells.

Kim, Man Sub,Kim, Jung Hee,Bak, Yesol,Park, Yun Sun,Lee, Dong Hun,Kang, Jeong Woo,Shim, Jung-Hyun,Jeong, Heon Sang,Hong, Jin Tae,Yoon, Do Young Lawrence Erlbaum Associates, Publishers [etc.] 2012 Nutrition and cancer Vol.64 No.8

<P>The Maillard reaction is a chemical reaction occurring between an amino acid and a reducing sugar, usually requiring thermal processing. Maillard reaction products (MRPs) have antioxidant, antimutagenic, and antibacterial effects, and although 2,4-bis (p-hydroxyphenyl)-2-butenal (HPB242), a fructose-tyrosine MRP, appears to inhibit proliferation of cancer cells, its mechanism of action has not been studied in detail. We found that HPB242 treatment modulated expression of cyclins and tumor suppressor genes in SiHa human cervical cancer cell lines: cyclins and phospho-pRB were downregulated, whereas the expression of CDK inhibitors and p53 was enhanced. HPB242 induced apoptosis dose-dependently by suppressing E7 expression and leading to sub-G1 cell-cycle arrest in SiHa cell lines; treatment also led to the proteolytic cleavage of caspase-3, -9, and poly (ADP-ribose) polymerase. Moreover, HPB242 upregulated Fas expression, altered expressions of pro- and antiapoptotic factors, and also inhibited nuclear translocation of nuclear factor κB and phosphorylation of IκB. HPB242 treatment decreased phosphatidyl inositol-3 kinase and p-Akt expression levels, demonstrating that this survival pathway may also be inhibited by HPB242. Cumulatively, HPB242 promotes apoptosis by influencing E7 expression, inducing cell-cycle arrest at sub-G1 phase, and promoting both intrinsic (mitochondrial) and extrinsic (Fas-dependent) apoptosis in SiHa human cervical cancer cells.</P>

High-throughput screening of protein variations in soybean seeds.

Vijayanand Velusamy,Kyung Jun Lee,Bo-Keun Ha,Jin Baek Kim,Sang Hoon Kim,Si-Yong Kang,Dong Sub Kim 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07

The protein in soybean seeds accounts for approximately 40% of the dry seed weight. Two major storage proteins, 7S and 11S, constitute 70-80% of the total storage proteins in the seeds. In this study, the variation of total soluble protein extracts from 1152 soybean landraces that have been collected from South Korea were studied using high-throughput screening method with HT Protein Express Labchip (Caliper Life Sciences, Inc.). Seven distinct protein band patterns - four protein sub-units of 11S and three sub-units of 7S, were taken into account and their presence or absence were analyzed. Among the 1152 landraces, 525 genotypes were identified as lacking lipoxygenase, 255 lacking α1 subunit, 680 lacking α subunit, 169 lacking β subunit, 140 lacking acidic, 114 lacking Kunitz Trypsin Inhibitor (KTi) and 199 lacking basic protein patterns. The high-throughput protein analysis is helpful in screening a large number of populations with less time and minimum labor. The selected genotypes with low amounts or lacking of anti-nutritional factors such as trypsin inhibitor, lipoxygenase and α subunit would be used for future breeding purpose of quality improvement in soybean protein.

GPS/GPR을 이용한 저수지 준설능력 향상

이재원,이동락,엄장섭 東亞大學校 建設技術硏究所 2008 硏究論文集 Vol.31 No.2

일반적으로 퇴적물 준설에 대한 조사는 GPS/Echo Sounder 및 Total Station/Echo Sounder를 조합하여 대상지의 수평위치 및 수심에 대한 관측을 실시하고 있으며, 하상의 단면계산 저수량 및 준설계획량 등을 추정 이후 준설계획에 대한 계획고 및 준설토량에 대한 세부적인 계획을 수립하게 된다. 하지만 Echo Sounder를 사용하는 음파탐사 방법은 퇴적물에 대한 정확한 분포를 파악할 수 없는 단점이 있다. 본 연구는 저수지 하부의 퇴적물 및 이의 분포를 파악하여 저수지 준설계획을 수립하여 기존의 탐사방법에 대한 준설능력 향상에 중점을 두었으며, 기존의 음파탐사 방식과 다른 레이더탐사 장비를 이용한 GPS/GPR(Ground Penetration Radar)을 사용하여 저수지의 수평위치, 수심값을 결정하고 동시에 원 지반에 분포하고 있는 퇴적물의 분포를 추정, 준설에 적용하고자 하였다. 우선 현장의 시료채취를 통한 수조모형실험을 실시하여 장비에 대한 정확도 검증을 거친 후 현장적용에 적용하여 그 가능성을 평가하였다.

동적스위칭평면을 포함한 출력궤환 가변구조제어계의 강인성설계

李起常,金東涉 단국대학교 1993 論文集 Vol.27 No.-

The output feedback variable structure control system(OFVSCS) proposed to remove the assumption of full state availability that is the most important restrictions to the practical application of state feedback VSCS is dealt with and two new propositions are presented. Firstly, an output feedback switching control input for the OFVSCS with dynamic switching surfaces is suggested. Secondly, a robust OFVSCS(ROFVSCS) is proposed. The ROFVSCS is composed of dynamic switching surfaces and output feedback switching control inputs that are constructed by the use of the unknown vector modeling technique. With the proposed scheme, existence of sliding mode is guaranteed and any effects of disturbances and/or process parameter variations can be suppressed as far as well-known matching condition is satisfied. Due to the fact that the ROFVSCS is driven by small number of measured outputs, it makes the practical application of VSCS for the systems with unmeasurable states and for high order systems that conventional schemes can not be applied possible. It is noticeable that the ROFVSCS can be constructed so as to have shorter reaching phase than that of the conventional VSCS and that the implementation cost of VSCS can be considerably reduced without deterioration of control performance by adopting ROFVSCS since there is no need to measure the states with high measurement cost in the scheme.

고온 폐가스 여과를 위한 집진기술

이동섭 경운대학교 산업기술연구소 2001 産業技術硏究論文誌 Vol.3 No.2(A)

Hot gas media filtration for temperature above 300℃ and mainly up to 600℃ is an evolving technology becoming more and more popular in different industry segments. With ever tightening restrictions on discharge emission levels all process industries are having to review their present operations and incorporate means of reducing to a minimum heir effluent discharges Bench scale candle filters (60 O.D×10t×300L) were tested using different dusts collected from many industries including paper, glass and cement manufacturing plants. Collection efficiencies range 99.67% for below 1㎛ diameter and 99.9% for above 1㎛ diameter particles. Darcy's law coefficients range 0.5×10^11 to 2×10^11/㎡ depending on manufacturing conditions and composition of binding materials. Detachment and filtration of articles were performed effectively using 3bar pulsing pressure, 300msec pulse jet valve opening time, 5min filtration cycle, 1g/min particle loading and 10㎝/sec face velocity.

탄화규소 세라믹 필터를 이용한 여과기술에 관한 연구

이선하,이동섭 경운대학교 산업기술연구소 2000 産業技術硏究論文誌 Vol.3 No.1(B)

Candle type SiC filters applicable to hot gas filtration of IGCC and PFBC were manufactured and tested using two different dusts collected from cokes and water glass manufacturing processes. Particle sizes, porosities, and mean pore sizes of manufactured SiC filters range 150∼300㎛, ∼40%, and 43∼83㎛, respectively. Collection efficiencies of two different types of dusts rage 99.09∼99.95% at 600℃ and latm test condition. At the same condition, Darcy's resistance coefficients rage 1.149×10^10∼3.514×10^11m^-2 and differential pressures range 187∼391㎜H₂O. Also it was found that the smaller the mean pore size, the higher the differential pressure at the same operating conditions. SiC filters have high potential as a fine dust removal medium from HTHP processes like PFBC and IGCC. Furthermore, long-term performance tests of the filters are necessary for the commercialization of SiC filters.

통보형 XML 공문서 관리를 위한 프레임웍 설계

이소영,정옥란,조동섭 한국컴퓨터교육학회 2004 컴퓨터교육학회 논문지 Vol.7 No.2

학교 내의 문서 전달은 교육청과 같은 규모가 큰 교육기관과는 달리 소규모라는 점 때문에 소흘히 여겨져 왔다. 외부의 행정 환경 변화에 따라 학교 내의 행정 서비스 전달 체계도 그 구조와 틀을 쇄신할 필요가 있다. 이러한 취지에서 본 논문은 실제 학교 현장에서 활용할 수 있도록 통보형 XML 공문서 관리를 위한 프레임웍을 설계하고, 컴포넌트 관계로 모델링하여 JSP 컴포넌트 기반의 공문서 관리 시스템을 구현하였다. 결과적으로 본 시스템은 기한이 임박한 공문서와 같은 교육활동에 부정적 영향을 미치는 요소를 줄이고 문서 관리 체계의 전산화로 학교 내 행정 서비스 환경을 개선한다는데 의의가 있다. Delivering documents within school itself, has been ignored, since it is not considered as a large organization such as government education agencies. It is necessary to change the procedures in delivering official documents within school's administrative services along with the outside administrative procedure changes. For the above purposes, this study can be practiced toward the actual schools therefore it is to design the framework for Distributable Official XML Document management and to implement official document management system based on JSP component. As a result, this system will reduce the negativity in producing the time critical official documents in educational activities. It is important at the fact that electronic document management procedures will incredibly improve the quality in school's administrative services.