DJ-1/park7 modulates vasorelaxation and blood pressure via epigenetic modification of endothelial nitric oxide synthase

Won, Kyung Jong,Jung, Seung Hyo,Jung, Soo Hyun,Lee, Kang Pa,Lee, Hwan Myung,Lee, Dong-Youb,Park, Eun-Seok,Kim, Junghwan,Kim, Bokyung Oxford University Press 2014 Cardiovascular research Vol.101 No.3

<P><B>Aims</B></P><P>DJ-1/park7, a multifunctional protein, may play essential roles in the vascular system. However, the function of DJ-1/park7 in vascular contractility has remained unclear. The present study was designed to investigate whether the DJ-1/park7 is involved in the regulation of vascular contractility and systolic blood pressure (SBP).</P><P><B>Methods and results</B></P><P>Norepinephrine (NE) elevated contraction in endothelium-intact vessels in a dose-dependent manner, to a greater extent in DJ-1/park7 knockout (DJ-1/park7<SUP>−/−</SUP>) mice than in wild-type (DJ-1/park7<SUP>+/+</SUP>) mice. Acetylcholine inhibited NE-evoked contraction in endothelium-intact vessels, and this was markedly impaired in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP>. Nitric oxide (NO) production (82.1 ± 2.8% of control) and endothelial NO synthase (eNOS) expression (61.7 ± 8.9%) were lower, but H<SUB>2</SUB>O<SUB>2</SUB> production (126.4 ± 8.6%) was higher, in endothelial cells from DJ-1/park7<SUP>−/−</SUP> mice than in those from DJ-1/park7<SUP>+/+</SUP> controls; these effects were reversed by DJ-1/park7-overexpressing endothelial cells from DJ-1/park7<SUP>−/−</SUP> mice. Histone deacetylase (HDAC)-1 recruitment and H3 histone acetylation at the eNOS promoter were elevated and diminished, respectively, in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP> controls. Moreover, SBP was significantly elevated in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP> controls, but this elevation was inhibited in mice treated with valproic acid, an inhibitor of Class I HDACs including HDAC-1.</P><P><B>Conclusion</B></P><P>These results demonstrate that DJ-1/park7 protein may be implicated in the regulation of vascular contractility and blood pressure, probably by the impairment of NO production through H<SUB>2</SUB>O<SUB>2</SUB>-mediated epigenetic inhibition of eNOS expression.</P>

공원녹지의 합리적 조성과 개선방안에 관한 연구 : 서울 특별시를 중심으로 A case study of Seoul

김동찬,박율진 경희대학교 부설 식량자원개발연구소 1993 硏究論文集 Vol.14 No.-

In order to present the basic data on the rational prep-plan and the fundamental conception with regards to the city park of Seoul, the improvement plan and fundamental conceptions are herewith presented after examining the present stats and problems of Seoul city's park. 1. For the state of park greens as of the end of 1992, Seoul has 1,348 parks covering 150.4 square kilometers which is equivalent to 13.71 square meters per person who inhabits in the city. 2. For the problems to be solved on the park green, laying the park sites disproportionately to the outskirts, the excessive numbers of non-furnished parks, the shortage of special faculties and so forth are listed. 3. As for the improvement plan regarding the park greens' problems, the fractionalizing of city park classification, the suppression of usage change of natural green spaces, the revision of regulations regarding the securing of city park facilities and sites in the park related regulations, the new installation of management post and park greens, the training and disposition of special manpowers, the activation of local resident participation in the administrative organization of park greens, the preparation of various space facilities, the execution of civil consignment of management, the expansion of park to new city center etc in the park preparation and operations are listed.

주차원단위 산정 모형 개발에 관한 연구 -광주광역시 공동 주택 아파트를 대상으로-

권성대,고동봉,박제진,하태준,Kwon, Sung-Dae,Ko, Dong-Bong,Park, Je-Jin,Ha, Tae-Jun 대한토목학회 2014 대한토목학회논문집 Vol.34 No.2

도시의 급격한 팽창과 함께 주택부족 현상이 나타나게 되자, 정부는 주택부족 문제 해결을 위해 대규모 택지개발을 통하여 주택보급을 확대시켰다. 이러한 현상으로, 공동주택은 우리나라 전체 주택의 83% 수준을 유지하고 있고, 그 중 아파트가 차지하는 비중은 50%로 꾸준한 증가 추세를 보이고 있다. 이로 인해 아파트의 경우 입주민들의 승용차 보유 증가에 따른 아파트 단지 내 주차공간 부족문제 등 제반 주차 관련 문제가 발생하고 있다. 특히, 주차계획대수 수립 시 교통영향평가의 주차수요예측 중 전용면적을 고려한 주차원단위 산정 방법은 기존 계획보다 세대수는 증가하여도 전용면적이 작아지면 계획주차대수는 감소하는 것으로 나타나, 보다 현실적인 주차원단위 산정이 필요한 실정이다. 이에 본 연구는 공공주택 아파트를 대상으로 현실에 적합한 주차원단위를 산정하고자 한다. 현장조사 및 설문조사를 실시하고, 구득자료에 대한 분석을 수행함으로써, 기존 교통영향평가의 주차원단위 산정 문제점을 도출하였다. 또한, 주차수요예측에 영향을 미치는 요인 선정을 통해 주차원단위 산정모형을 개발하였다. 마지막으로 실제 조사된 아파트 주차원단위 자료를 통해 기존 교통영향평가의 주차원단위 산정과 본 연구에서 제시한 주차원단위 산정모형을 비교 분석하였다. 향후 본 연구에서 개발된 주차원단위 산정모형은 주차장법 기준 정립은 물론 보다 현실적인 주차수요예측 수행에 적극 활용될 수 있을 것으로 판단된다. The rapid expansion of cities led to the shortage of housing in urban areas. The government compensated for this shortage through large scale residential developments that increased the housing supply. The supply of condominium apartments remains above 83% of the entire housing supply, and the proportion of apartments are at a steady increase, at about 50%. Due to the increase, illegally parked cars resulting from the shortage of parking spaces within the apartment complex have become increasingly problematic as they block the transit of emergency vehicles, and heighten the tension among neighboring residents in obtaining a parking space. Especially, the future residents are considered to plan the parking based on the estimated demand for parking. However, the parking unit method utilized to estimate the parking demand accounts for the exclusive use of space, which is believed to be far from the parking demands in reality. The reason for this discrepancy is that, as the number of households decrease, and area of exclusive space is expanded, the planned parking increases. On the other hand, when the number of households increase, and the area of exclusive space is reduced, the planned parking decreases, thus methods to recalculate the parking units based on estimated parking demand is an urgent concern. To estimate the parking units based on condominium apartments, this study first examined the existing research literature, and appointed the field of investigation to collect the necessary data. In addition, field study data and surveys collected and analyzed, in order to identify the problems underlying parking units, and problems regarding the current traffic impact assessment parking unit calculation method were deduced. Through identifying the influential factors on parking demand estimates, and performing a factorial analysis based on the collected data, the variables were selected in relation to the parking demand estimates, to develop the parking unit estimate model. Finally, through comparing and verifying the existing traffic impact assessment parking unit estimate against the newly developed model using collected data, a far more realistic parking unite estimate was suggested, reflecting the characteristics of the residents. The parking unit estimate model developed in this study is anticipated to serve as the guidelines for future parking lot legislature, as wel as the basis to provide a more realistic estimate of parking demands based on the resident characteristics of an apartment complex.

민간공원특례사업의 추진에 따른 사업특성에 관한 연구

권영달,박현빈,김동필,Gweon, Young-Dal,Park, Hyun-Bin,Kim, Dong-Pil 한국조경학회 2021 韓國造景學會誌 Vol.49 No.5

본 연구는 도시공원 일몰의 대응수단으로 추진 중인 전국의 민간공원특례사업을 대상으로 지자체 및 공원현황, 사업특성과 시행 등에 관련된 내용을 세부적으로 분류 후 비교·분석하여 제도시행 후 진행되어진 지자체별 사업을 점검하고, 그 가운데 제도의 의미와 보완점을 구축하고자 실시하였다. 분석결과로서 첫째, 전국에서 시행 중인 민간공원특례사업은 주로 인구 10만명 이상의 도시에서 시행되어 군 지역이나 지방소도시의 적용에는 한계성이 있었다. 이에 특례제도를 일괄적으로 적용하기보다 지자체의 특성과 규모를 고려한 제도 적용의 유연성이 필요할 것으로 판단되어졌다. 둘째, 공원조성 기부채납 방식에 의한 현재의 특례사업은 공동주택 위주의 단조로운 개발유형을 나타내고 있어, 이의 개선을 위해 공원부지만 매입하여 기부 채납하는 공원 보전형 방식 등을 도입하여 개발의 다양화를 도모할 필요가 있는 것으로 나타났다. 셋째, 공원의 유형과 면적이 한정되어 사업대상에 한계를 보이고 있어, 대도시 도심지내 이용도가 높고 접근성이 좋은 공원들까지 포함할 수 있도록 면적기준을 5만m² 이하까지 완화할 필요가 있는 것으로 나타났다. 넷째, 특례사업의 대상지가 대부분 산지형 공원으로 자연지형 및 스카이라인의 훼손 우려가 있어 공원별 입지특성을 고려한 건폐율과 용적률을 별도 조례를 설정하여 적용하는 것과 비공원시설 유형별 건축 가이드라인을 세부적으로 설정하여 공원과 공존할 수 있는 개발접근이 이루어져야 할 것이다. 향후 과제로는 첫째, 전국의 민간공원특례사업이 완료된 후, 각 사업별 수익률을 데이터화 하여 향후 유사사업의 적정수익률에 대한 기준을 설정하고, 초과이익분의 환수 등 제도적 기준을 마련하며, 둘째, 사업기간의 단축을 위해 지자체별 TF팀 구성, 통합심의 전환, 제안단계에서 환경성 검토 도입 등이 검토되어야 할 것이다. 셋째, 지자체는 민간협의체를 구성을 제도화하여 협치시스템을 통한 사업의 효율적 관리를 도모하고, 넷째, 특례공원의 기부채납 이후 유지관리에 대한 로드맵을 수립하여, 시민이 함께 하는 시민 참여형 공원 운영·관리방안 모델의 도입을 검토하여야 할 것으로 나타났다. This study was conducted to examine and analyze local governments, park status, project characteristics, and the implementation in detail for private park special projects across the country as a means of responding to the sunsetting of urban parks. As a result of the analysis, first, the private park special project, was found to be mainly implemented in cities with a population of more than 100,000, so there was a limit to the application on military installations or in local small cities. Therefore, rather than applying the special system collectively, it was judged that institutional flexibility, considering the characteristics and size of local government, was needed. Second, the current special projects by the park creation donation collection method shows monotonous development centered on apartment houses, so it is necessary to diversify the development by introducing a park preservation method that purchases and donates park sites. Third, it was found that the area standard needs to be eased to less than 50,000m² to include parks with high utilization and good accessibility in urban areas of large cities, as the type and area of parks are limited. Fourth, most special projects are mountain parks, which are feared to damage the natural terrain and skyline, so separate ordinances should be established and applied, and development approaches should be made to allow nature and parks to coexist with the setting of detailed building guidelines for each type of facility. The guidelines should include, first, after the nationwide private park special projects are completed, standards for appropriate returns for similar projects should be established, institutional standards such as the recovery of excess profits should be established, and environmental reviews should be conducted. Second, it was found that local governments should institutionalize the composition of private consultations to promote the efficient management of projects through a cooperative system, and third, a roadmap for maintenance after the donation of special parks should be established.

12M의 고해상도 360° 카메라를 사용한 주차장의 14면 주차 상태 판단 프로그램

이영지(Young-Ji Lee),이희열(Hee-Yeol Lee),고태영(Tae-Young Ko),곽동훈(Dong-Hoon Kwak),김재형(Jae-Hyung Kim),김주호(Joo-Ho Kim),오승진,이태윤(Tae-Yoon Lee),박상민(Sang-Min Park),이승호(Seung-Ho Lee) 대한전자공학회 2019 대한전자공학회 학술대회 Vol.2019 No.6

In this paper, we propose a program to identify 14 parking status in a parking lot using a high resolution 360° camera of 12M. The proposed program consists of three steps: Match with plane image, parking area detection algorithm, and discrimination of parking using learning method. Tests on a model car to evaluate the program to identify 14 parking status in a parking lot using a high-resolution 360° camera of 12M showed 100% accuracy for both parking and double parking. Therefore, the effectiveness of a program to identify 14 parking status in a parking lot using a high-resolution 360° camera of 12M proposed in this paper has been proved.

지리산국립공원 내 산림관리에 관한 전문가 인식 조사

김동현 ( Dong Hyeon Kim ),김의경 ( Eui Gyeong Kim ),박상병 ( Sang Byeong Park ),이정환 ( Jeong Hwan Lee ) 한국산림과학회 2010 한국산림과학회지 Vol.99 No.4

This study carried out the recognition of appropriate forest managements and directions in Jirisan National Park. For this purpose, we undertook interview survey about the recognition of forest management in Jirisan National Park from ecological and forest research fields` experts. From the statistical analysis results, it was founded that the difference among respondents` types was detected about recognition of forest management in National park. However, all the respondent types recognized that the forest management in National park is essential. So the forest management in national park by forest experts and relevant institutions are revealed needed.

Brief introduction of Butterfly(Lepidoptera) from Myanmar

Ja Lang LIM,Jinyoung PARK,Ik Je CHOI,Yong Kyun OH,Eui Seok JEONG,Dong Pyeo LYU,U Pho Cho,Khin Mar Myint,Khin Mar Yi,Jong Kyun PARK 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.04

Myanmar is located in the south-east Asia peninsular and it is flanked by five neighboring states: Bangladesh, India, China, Laos and Thailand. It is located 10~22 degree north latitude, to 92~101 degrees east longitude. Myanmar has a tropical climate with three seasons in Myanmar. Hot season (March through April), rainy season (May through October) and the cool season (November through February). The coastal regions can get very hot and humid, especially before the rainy season. Myanmar has a monsoon season from May to October when there are heavy rains and often flooding. The investigation was carried out during the dry season(2013) in Nat Ma Taung National Park and Popa Mountain Park. Popa Mountain Park is located in Kyawpadaung Township, Myinchan district, Mandaly Division. The area is 49.63 square miles. Its coordinates are 29°31'60" N and 27°43'60" E. Nat Ma Taung National Park, Myanmar treasure is in Mindut District of Chin State and its area of 279 square miles. It is the third highest mountain in Myanmar with the height of 10,500 feet. Its coordinates are 21°14'00" N and 93°54'00" E. As the result, butterflies(Lepidoptera) collected in the two regions were about 60 species of 7 familys. Collecting dates, expedition activities, photo of main specimen and information of Myanmar were provided.

한국인 폐확산능 정상예측식의 임상적 유용성과 정확성

나승원 ( Seung Won Ra ),박태선 ( Tai Sun Park ),홍윤기 ( Yoon Ki Hong ),홍상범 ( Sang Bum Hong ),심태선 ( Tae Sun Shim ),임채만 ( Chae Man Lim ),이상도 ( Sang Do Lee ),고윤석 ( Youn Suck Koh ),김우성 ( Woo Sung Kim ),김동순 ( Dong 대한결핵 및 호흡기학회 2008 Tuberculosis and Respiratory Diseases Vol.64 No.2

연구배경: 폐확산능을 해석하는 데 필요한 정상예측식으로는 한국인을 대상으로 하여 박 등이 개발한 식(박 식)이 있으나 아직 외국 정상예측식을 많이 사용하고 있다. 이에 국내에서 많이 사용하는 외국 정상예측식인 Burrows 식과 박 식의 임상적 유용성과 정확성을 비교하고자 하였다. 방법: 1. 임상적 유용성 연구; 2006년 7월부터 12월까지 6개월간 폐확산능검사를 시행한 환자 중 두 식을 각각 정상예측식으로 적용하였을 때 폐확산능 해석이 다른 276명(대상군 A)을 대상으로 하였다. 대상군 A에게 두 식을 각각 적용하였을 때 폐확산능 해석과 임상적 판단과의 일치도를 비교하여 임상적 유용성을 평가하였다. 2. 간질성폐질환 진단의 정확성 비교; 2001년부터 2006년까지 폐조직검사를 시행하여 확진된 간질성폐질환군과 서울아산병원에서 모집한 정상군을 대상으로 하여 정상예측식으로 두 식을 각각 적용하였을 때 폐확산능 해석의 정확도를 비교하였고, 두 식이 차이가 나는지 맥니머의 카이스퀘어 검정을 하였다. 결과: 1. 임상적 판단과의 일치도 비교; 276명을 임상정보를 토대로 폐확산능을 예측하여 분류한 결과 정상 54명, 감소 220명, 불분명이 2명이었다. 예측식으로 박 식과 Burrows 식을 적용하였을 때 임상적 판단과 일치하는 환자는 각각 78%와 22%이었다(p<0.001). 2. 간질성폐질환 진단의 정확성 비교; 박 식은 민감도 90.1%, 특이도 100%이었고 Burrows 식은 민감도 64.2%, 특이도 100%로 민감도가 통계학적으로 유의하게 박 식이 높았다(p<0.001). 결론: 우리나라 정상예측식인 박 식이 외국 정상예측식인 Burrows 식을 정상예측식으로 적용하는 것보다 임상적 유용성이나 간질성폐질환 진단의 민감도에서 더 우월하였다. 향후 폐확산능검사의 정상예측식으로 박 식을 사용해야 할 것으로 사료된다. Background: Park et al. developed the Korean reference equation for the measurement of diffusing capacity in 1985. However, the equation has not been widely used in Korea and foreign reference equations have been popularly used. We intended to compare the clinical usefulness and the accuracy of the the Korean reference equation (Park`s equation) with that of the foreign equation (Burrows` equation) that is commonly used in Korea. Methods: 1. Evaluation of clinical usefulness; Among 1,584 patients who underwent diffusing capacity (DLCO) at the Asan Medical Center from July to December 2006, group A subjects included 276 patients who had different interpretations of DLCO in trials employing Burrows` equation and Park`s equation. Clinical assessment was decided by consensus of two respiratory physicians. In order to evaluate the clinical usefulness of Burrows` equation and Park`s equation, agreement of clinical assessment and DLCO interpretation were measured. 2. Evaluation of accuracy; Group B subjects were 81 patients with interstitial lung disease (ILD) and 39 normal subjects. The 81 ILD patients were diagnosed following a surgical lung biopsy. The accuracy of diagnosing ILD as well as sensitivity and specificity were evaluated according to the use of the reference equations (Burrows` equation and Park`s equation) for DLCO. Results: Agreement between clinical assessment and interpretation of DLCO was 22% for the use of Burrows` equation and 78% for the use of Park`s equation. The sensitivity and specificity of the Burrows` equation for diagnosing ILD were 64.2% and 100%. The sensitivity and specificity of the Park`s equation for diagnosing ILD were 90.1% and 100%. The sensitivity of the Park`s equation for diagnosing ILD was significantly higher than that of Burrows` equation (p<0.001). Conclusion: The Korean reference equation (Park`s equation) was more clinically useful and had higher sensitivity for diagnosing ILD than the foreign reference equation (Burrows` equation). (Tuberc Respir Dis 2008;64:80-86)

공원녹지관리 운영실태 및 의식구조에 관한 연구 : 인사관리측면을 중심으로

박율진,김동찬 경희대학교 지역문제연구부 1996 지역개발연구 Vol.3 No.-

This study is based on the investigation of the actual conditions of management and the comparative analysis of related actions of parks and green spaces in local autonomous entities (six cities). For conducting this research, questionnaires were used. The research findings are summarized as follows and they could be used for improving the quality of management systems of urban parks and green spaces. Meanwhile, this study's findings are in brief as following; in case of employment and promotion, research the new member of forest section during 3 years(1992-1994) in order to Seoul 57 persons(38.4%), Busan 45 and KwangJu 4 persons(2.7%). DaeGu and DaeJeon's employment had equalbalance according to the local. Also, application had an unbalance of the personnel structure about position balance so that's why so many low position worker engagement. In case of educational training and consideration of professional, member's professional improvement required re-education and have to re-set up the curriculum that all self government groups depend on office of forestry except Seoul city. In case of consciousness tendency analysis, member's working desire requirement(4.60), professional worker for the park and green administration requirement(4.60) were showed so high. Efficiency of position management(2.39), Structure discrepancy of personnel management system(2.45), functional worker shortage of parks and green space(2.40) and etc. were showed low. In evaluative model analysis, for the efficiency of managing system, member's work desire, proper educational training, discrepancy of personnel management system discrepancy, professional worker required for the park and green space, working circumstance, personnel evaluation according to task achievement via educational training course relatively.

기계식 주차설비 관제정산 시스템 개발

최동진,김태효,박영석 慶南大學校 附設 工業技術硏究所 1999 硏究論文集 Vol.17 No.-

본 논문에서는 기존 주차설비 자동화 기술의 문제점을 극복하기 위해서 자동 주차타워 관리용 관제정산 시스템의 객체지향 개발접근법을 제시한다. 개발된 주차설비 관제정산 시스템은 주차설비 제어장치와 정산 장치를 직렬 통신으로 결합한 하드웨어와 관제정산 소프트웨어로 구성된다. 관제정산 소프트웨어는 GUI 환경 하에서 객체지형 분석법을 통해 설계되고 Delphi 개발도구를 통해 구현되었다. 기존 시스템과는 달리 주차설비 조작, 주차증발행 및 인식, 요금정산 등을 일체화함으로써 사용상의 번거로움과 불편을 해소하고, 주차설비의 작동상태를 실시간으로 모니터링함으로써 높은 안전성을 제공한다. 또한 주차차량정보 및 주차관리기능을 통해서 주차 요금정산의 정확성 및 신뢰성 향상을 크게 기대할 수 있다. In this paper. in order to overcome the problems of the current automated parking systems, we propose an object-oriented development method of the control and account system for the management of automated parking tower. The developed system consists of the hardware, including the account unit and the parking control unit, and the control and account software. The hardware units are connected by serial communication line. The control and account software was designed through the object-oriented analysis approach and implemented by using Delphi development tool under GUI environment. Our system can minimize the difficulties and inconvenience of the use because of functioning as a total system, and provide the high safety of the parking operation by monitoring the parking status in real time. A1so it can greatly improve the correctness and reliability of accounting the parking fee.