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Dong Gen Piao,이유정,서창섭,이종순,김재룡,장현욱,손종근 한국생약학회 2011 Natural Product Sciences Vol.17 No.3
Eight compounds, squalene (1), friedelin (2), b-sitosterol (3), b-sitosterol-3-O-glucoside (4), a-tocopherol (5), betulinic acid (6), trilinolein (7) and 1-O-(9Z,12Z-Octadecadienoyl)-3-nonadecanoyl glycerol (8), were isolated from the barks of Tilia amurensis. Their chemical structures were identified by comparing their physicochemical and spectral data with those published in the literature. These isolated compounds were examined for their inhibitory activities against topoisomerase I and II. Compound 7 showed significant inhibition of DNA topoisomerase I and II activities, with percent decreases in activity of 87 and 95%, respectively at a concentration of 100 mM. Compound 6 exhibited cytotoxicity against the human colon adenocarcinoma cell line (HT-29), the human breast adenocarcinoma cell line (MCF-7) and the human liver hepatoblastoma cell line (HepG-2), with IC50 values of 20, 59 and 16 mM, respectively.
Quality evaluation of Carthami Flos by HPLC–UV
Ying LI,Dong Gen Piao,HaiYan Zhang,김태인,이승호,장현욱,우미희,손종근 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.5
A HPLC–DAD method was developed forsimultaneous determination of four marker compounds,kaempferol-3-O-rutinoside, safflomin A, safflomin B andbidenoside C, in the extract of the flowers of Carthamustinctorius Linne. Natural samples were extracted in 50 %aqueous methanol by ultra-sonication for 60 min. Markercompounds were separated on a C18 column by two-stepgradient elution of mobile phase (acetonitrile/water) at aflow rate of 0.75 mL/min and detected at 210 nm. Theretention times of safflomin A and safflomin B were shiftedaccording to the pH of the mobile phase. The optimizedanalytical method was validated to confirm linearity, precision,accuracy and stability of marker compounds. Themethod was successfully employed to analyze 17 naturalsamples from different regions, and the data matrix wasmonitored and visualized by principal component analysis. The assay method could be applied for quality control ofthe flowers of C. tinctorius Linne.
Ying Li,Dong Gen Piao,Haiyan Zhang,우미희,이제현,문동철,이승호,장현욱,손종근 대한약학회 2013 Archives of Pharmacal Research Vol.36 No.3
Cynanchum auriculatum and Cynanchumwilfordii are widely used as folk medicine in Eastern Asia. However, the indeterminacy in the authentic original plantmaterial has resulted in the same appellative name beinggiven to the two plants, and they are commonly misused. Therefore, it is necessary to establish an analytical methodfor discrimination as well as quality control of the twospecies. This study was to develop HPLC–UV methods forquality assessment of C. auriculatum and C. wilfordii anddiscrimination between the two species. Two HPLCmethods to analyze eight marker compounds were establishedand validated. The first method analyzed sevenmarker compounds simultaneously on a reversed-phasecolumn, while the second method analyzed a single markercompound, conduritol F, which exists only in C. wilfordii, on a Si-column. Thirty-nine batches of C. auriculatum andnineteen batches of C. wilfordii that were collected fromdifferent geographical regions of South Korea were analyzedby these methods. The constructed data matrix wassubjected to principal components analysis and hierarchicalcluster analysis in order to classify the samples. Theestablished methods offer a potential strategy for authenticationand differentiation of the two species.
Medicinal Chemistry : RESEARCH ARTICLE ; Quality evaluation of Carthami Flos by HPLC-UV
( Ying Li ),( Dong Gen Piao ),( Hai Yan Zhang ),( Taein Kim ),( Seung Ho Lee ),( Hyeun Wook Chang ),( Mi Hee Woo ),( Jong Keun Son ) 영남대학교 약품개발연구소 2015 영남대학교 약품개발연구소 연구업적집 Vol.25 No.-
A HPLC-DAD method was developed for simultaneous determination of four marker compounds. Kaempferol-3-O-rutinoside. safflomin A. saffllomin B and bidenoside C , in the extract of the flowes of Carthamustinctorius Linne. Natural samples were extracted in 50% aqueous methanol by ultra-sonication for 60 min. Marker compounds were separated on a C18 column by two-step gradient elution of mobile phase (acetonitrile/water) at a flow rate of 0.75mL/min and detected at 210nm. The retention times of sfflomin A and safflomin B were were shifted according to the pH of the mobile phase. The optimized analytical method was validated to confirm linearity, precision accuracy and stability of marker compounds. The method was successfully employed to analyze 17 natural samples from different regions, and the data matrix was monitored and visualized by principal component analysis. The assay method could be applied for quality control of the flowers of C. tinctorius Linne.
Kyung Min Park,손종근,Ying Li,Bora Kim,Haiyan Zhang,Kyong Hwangbo,Dong Gen Piao,Mei Juan Chi,우미희,최재수,이제현,문동철,장현욱,김재룡 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.12
Two stable high-performance liquid chromatography (HPLC) methods were developed that could quantitatively analyze 10 major marker compounds of Artemisia capillaris Thunb and could also distinguish among ‘Injinho’ and ‘Myeon-injin’ and ‘Haninjin’ – A. capillaris collected in autumn,A. capillaris collected in spring and A. iwayomogi, which can be misused as ‘Injinho’ in Korean herbal drug markets. The first HPLC method was a reversed-phase chromatography using a C18 column with an isocratic solvent system of phosphoric acid (0.05%) and acetonitrile at the flow rate of 1.0 mL/min, ultraviolet (UV) detection wavelength at 254 nm and column temperature at 40oC. Calibration and quantitation were made by using acetaminophen as an internal standard (I.S-A) and chlorogenic acid (1) was determined within 20 min. The second HPLC method was a reversed-phase chromatography using a C18 column with a gradient solvent system of phosphate buffer (0.015 M, pH 6) and acetonitrile at the flow rate of 1.0 mL/min, UV detection wavelength at 254 nm and column temperature at 40oC. Calibration and quantitation were made by using ethylparaben as an internal standard (I.S-B) and 3,5-di-O-caffeoylquinic acid (2), 3,4-di-O-caffeoylquinic acid (3), 4,5-di-O-caffeoylquinic acid (4), hyperoside (5), isoquercitrin (6), isorhamnetin 3-O-robinobioside (7), isorhamnetin-3-O-galactoside (8), isorhamnetin-3-O-glucoside (9) and scoparone (10) were determined within 60 min. Pattern recognition analysis of data from the 60 samples classified them clearly into three groups. These assay methods could be applied for QA/QC of A. capillaris and Artemisia iwayomogi.
( Kyung Min Park ),( Ying Li ),( Bora Kim ),( Haiyan Zhang ),( Kyong Hwangbo ),( Dong Gen Piao ),( Mei Juan Chi ),( Mi Hee Woo ),( Jae Sue Choi ),( Je Hyun Lee ),( Dong Cheul Moon ),( Hyeun Wook Chang 영남대학교 약품개발연구소 2013 영남대학교 약품개발연구소 연구업적집 Vol.23 No.0
Two stable high-performance liquid chromatography (HPLC) methods were developed that could quantitatively analyze 10 major marker compounds of Artemisia capillaris Thunb and could also distinguish among `Injinho` and `Myeon-injin` and `Haninjin`--A. capillaris collected in autumn, A. capillaris collected in spring and A. iwayomogi, which can be misused as `Injinho` in Korean herbal drug markets. The first HPLC method was a reversed-phase chromatography using a C18 column with an isocratic solvent system of phosphoric acid (0.05%) and acetonitrile at the flow rate of 1.0 mL/min, ultraviolet (UV) detection wavelength at 254 nm and column temperature at 40°C. Calibration andquantitation were made by using acetaminophen as an internal standard (I.S-A) and chlorogenic acid (1) was determined within 20 min. The second HPLC method was a reversed-phase chromatography using a C18 column with a gradient solvent system of phosphate buffer (0.015 M, pH 6) and acetonitrile at the flow rate of 1.0 mL/min, UV detection wavelength at 254 nm and column temperature at 40°C. Calibration and quantitation were made by using ethylparaben as an internal standard (I.S-B) and 3,5-di-O-caffeoylquinic acid (2), 3,4-di-O-caffeoylquinic acid (3), 4,5-di-O-caffeoylquinic acid (4), hyperoside (5), isoquercitrin (6), isorhamnetin 3-O-robinobioside (7), isorhamnetin-3-O-galactoside (8), isorhamnetin-3-O-glucoside (9) and scoparone (10) were determined within 60 min. Pattern recognitionanalysis of data from the 60 samples classified them clearly into three groups. These assay methods could be applied for QA/QC of A. capillaris and Artemisia iwayomogi.
최재필(Choi, Jaepil),이정원(Lee, Jung-Won),안의순(Ahn, Eui-Soon),손동화(Shon, Dong-Hwa),박근송(Piao, Gen-Song) 대한건축학회 2015 대한건축학회논문집 Vol.31 No.6
The purpose of this research is to construct a classification system of biological information so that it can be applied to biomimicry architectural research. It is both uneconomical and time consuming for architectural researchers to spend time searching and studying principles of organisms. In this study, we suggested a classification system which includes biological information and can be used as a guide for architectural researchers attempting to research biomimicry architecture. Basic principles were extracted from architectural planning elements and biological information. Architectural experts matched biological principles to relevant architectural principles. Based on the architectural and biological principle correspondence table, group analysis was conducted. Biological principles were classified into 6 parts: air, structure/material, habitat, water/circulation, shape, and energy. This classification of biological principles corresponds to 6 architectural principles: ventilation, natural material, insulation, water resource, awning, and lighting/solar heat. According to the results of this research, specific classification groups may appear as biometric information increase through further research.