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RISS 인기검색어
- 검색키워드
- 저자 : ( Dilli Prasad Paudyal ) (검색결과 12 건)
- 무료
- 기관 내 무료
- 유료
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Paudyal, Dilli Prasad,Hyun, Jae-Wook The Korean Society of Plant Pathology 2015 Plant Pathology Journal Vol.31 No.4
Citrus scab disease is one of the destructive diseases that reduce the value of fruit for the fresh market. We analyzed the process of symptom development after infection with scab pathogen $Elsino{\ddot{e}}$ fawcettii in the susceptible satsuma mandarin leaves to observe the structural modification against pathogen. The cuticle and epidermal cells along with 3-5 layers of mesophyll tissue were degraded 1-2 days post inoculation. Surrounding peripheral cells of degraded tissues grew rapidly and then enveloped the necrotic area along with the growing conidia. Cross sections through the lesion revealed hyphal colonization in epidermis and mesophyll tissues. In response to the pathogen colonization, host cell walls were lignified, inner cells were rapidly compartmentalized and a semi-circular boundary was formed that separated the infected region from the non-infected region, and finally prevented the intercellular pathogen spread.
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Dilli Prasad Paudyal,현재욱 한국식물병리학회 2015 Plant Pathology Journal Vol.31 No.4
Citrus scab disease is one of the destructive diseases that reduce the value of fruit for the fresh market. We analyzed the process of symptom development after infection with scab pathogen Elsinoë fawcettii in the susceptible satsuma mandarin leaves to observe the structural modification against pathogen. The cuticle and epidermal cells along with 3–5 layers of mesophyll tissue were degraded 1–2 days post inoculation. Surrounding peripheral cells of degraded tissues grew rapidly and then enveloped the necrotic area along with the growing conidia. Cross sections through the lesion revealed hyphal colonization in epidermis and mesophyll tissues. In response to the pathogen colonization, host cell walls were lignified, inner cells were rapidly compartmentalized and a semi-circular boundary was formed that separated the infected region from the non-infected region, and finally prevented the intercellular pathogen spread.
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현재욱,Dilli Prasad Paudyal,황록연 한국식물병리학회 2015 식물병연구 Vol.21 No.3
Elsinoe fawcettii and E. australis are two currently recognized scab pathogens of citrus. E. fawcettii has at least six pathotypes while E. australis has at least two pathotypes. Colonies of E. fawcettii and E. australis do not sporulate in artificial media including potato dextrose agar (PDA). Whiteside’s method has been widely used for preparing conidial inoculum in vitro. This study was carried out to develop efficient method for conidia production from artificial media. We developed a shaking method which included the following steps: 1) Colony grown on PDA was mashed with a steel spatula; 2) Mycelia fragments were cultured in 50 ml sterilized rain water in a rotary shaker-incubator (180 rpm) at 25ºC for 24 h: 3) The conidia suspension was filtered through two layers of cheesecloth. Average conidia production of all isolates tested using this shaking method was approximately 13.1 times higher than that from Whiteside’s method in this study.
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Kihye Shin,Dilli Prasad Paudyal,Seong Chan Lee,Jae Wook Hyun 한국식물병리학회 2021 Plant Pathology Journal Vol.37 No.3
Citrus scab, caused by the fungal pathogen Elsinoë fawcettii, is one of the most important fungal diseases affecting Citrus spp. Citrus scab affects young tissues, including the leaves, twigs, and fruits, and produces severe fruit blemishes that reduce the market value of fresh fruits. To study the molecular responses of sat- suma mandarin (C. unshiu) to E. fawcettii, plant hor- mone-related gene expression was analyzed in response to host-compatible (SM16-1) and host-incompatible (DAR70024) isolates. In the early phase of infection by E. fawcettii, jasmonic acid- and salicylic acid-related gene expression was induced in response to infection with the compatible isolate. However, as symptoms advanced during the late phase of the infection, the jas- monic acid- and salicylic acid-related gene expression was downregulated. The gene expression patterns were compared between compatible and incompatible inter- actions. As scabs were accompanied by altered tissue growth surrounding the infection site, we conducted gibberellic acid- and abscisic acid-related gene expres- sion analysis and assessed the content of these acids during scab symptom development. Our results showed that gibberellic and abscisic acid-related gene expres- sion and hormonal changes were reduced and induced in response to the infection, respectively. Accordingly, we propose that jasmonic and salicylic acids play a role in the early response to citrus scab, whereas gibberellic and abscisic acids participate in symptom development.
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최석,Shankar Prasad Parajuli,정현숙,Dilli Parasad Paudyal,염철호,윤평진,전제열 대한약리학회 2007 The Korean Journal of Physiology & Pharmacology Vol.11 No.1
To investigate whether hydrogen peroxide (H2O2) affects intestinal motility, pacemaker currents and membrane potential were recorded in cultured interstitial cells of Cajal (ICC) from murine small intestine by using a whole-cell patch clamp. In whole cell patch technique at 30oC, ICC generated spontaneous pacemaker potential under current clamp mode (I=0) and inward currents (pacemaker currents) under voltage clamp mode at a holding potential of -70 mV. When ICC were treated with H2O2 in ICC, H2O2 hyperpolarized the membrane potential under currents clamp mode and decreased both the frequency and amplitude of pacemaker currents and increased the resting currents in outward direction under voltage clamp mode. Also, H2O2 inhibited the pacemaker currents in a dose-dependent manner. Because the properties of H2O2 action on pacemaker currents were same as the effects of pinacidil (ATP-sensitive K+ channels opener), we tested the effects of glibenclamide (ATP-sensitive K+ channels blocker) on H2O2 action in ICC, and found that the effects of H2O2 on pacemaker currents were blocked by co- or pre- treatment of glibenclamide. These results suggest that H2O2 inhibits pacemaker currents of ICC by activating ATP-sensitive K+ channels.
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Hyun, Jae-Wook,Paudyal, Dilli Prasad,Hwang, Rok-Yeon The Korean Society of Plant Pathology 2015 식물병연구 Vol.21 No.3
Elsinoe fawcettii and E. australis are two currently recognized scab pathogens of citrus. E. fawcettii has at least six pathotypes while E. australis has at least two pathotypes. Colonies of E. fawcettii and E. australis do not sporulate in artificial media including potato dextrose agar (PDA). Whiteside's method has been widely used for preparing conidial inoculum in vitro. This study was carried out to develop efficient method for conidia production from artificial media. We developed a shaking method which included the following steps: 1) Colony grown on PDA was mashed with a steel spatula; 2) Mycelia fragments were cultured in 50 ml sterilized rain water in a rotary shaker-incubator (180 rpm) at $25^{\circ}C$ for 24 h: 3) The conidia suspension was filtered through two layers of cheesecloth. Average conidia production of all isolates tested using this shaking method was approximately 13.1 times higher than that from Whiteside's method in this study.
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Influence of the SMT2 Knock-out on Hypocotyl Elongation in Arabidopsis thaliana
정현숙,황인덕,김성기,Dilli Prasad Paudyal 한국생물공학회 2007 Biotechnology and Bioprocess Engineering Vol.12 No.2
Inhibitors are very important in the study of hormone function. Brasinazole (Brz) is a specific inhibitor of brassinosteroids (BRs) biosynthesis. To expand our knowledge of the molecular mechanisms of plant steroid signaling, we performed genetic screening using medium containing Brz under dark conditions. Mutants insensitive to Brz develop longer hypocotyls than their wild type counterparts. We isolated abz453 as a Brz insensitive mutant. TAIL-PCR and the segregation ratio of T2 plants indicated a single T-DNA insertion at the 24-Sterol C-methyltransferase (SMT2) gene in the abz453 mutant. Recapitulation for putative FCP serine phosphatase (FSP), the gene neighboring SMT2, indicated no significant phenotypes, but the SMT2 anti-sense (SMT2-AS) line developed longer hypocotyls than the wild type in medium containing Brz. Additionally, the SMT2-AS line displayed similar phenotypes to the abz453 line in soil including enhanced growth and smaller silique. The abz453 and SMT2-AS mutants showed phenotypes similar to those of wild type in medium containing benzylaminopurine, pacrobutrazol and ACC (precursor for ethylene) under dark conditions. However, when brassinolide (BL) dose response was observed, the abz453 and SMT2-AS lines showed higher sensitivity than wild type. The abz453/det2 and abz453/bri1-119 double mutants showed enhanced growth compared to the det2 and bri1-119 line under both dark and light conditions. Specially, in dark conditions double mutants displayed nearly 2- and 1.5-fold longer hypocotyls than det2 and bri1-119 plants. Brz insensitivity to the SMT2 knock-out mutant and phenotypes of double mutants indicate that not only do BRI1 and DET2 influence the BRs response, as evidenced by hypocotyl elongation, but another sterol derived signals may also be affected in mutants, suggesting that another pathway is involved in hypocotyl elongation due to SMT2.
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Choi Seok,Parajuli Shankar Prasad,Cheong Hyeon-Sook,Paudyal Dilli Parasad,Yeum Cheol-Ho,Yoon Pyung-Jin,Jun Jae-Yeoul The Korean Society of Pharmacology 2007 The Korean Journal of Physiology & Pharmacology Vol.11 No.1
To investigate whether hydrogen peroxide($H_2O_2$) affects intestinal motility, pacemaker currents and membrane potential were recorded in cultured interstitial cells of Cajal(ICC) from murine small intestine by using a whole-cell patch clamp. In whole cell patch technique at $30^{\circ}C$, ICC generated spontaneous pacemaker potential under current clamp mode(I=0) and inward currents(pacemaker currents) under voltage clamp mode at a holding potential of -70 mV. When ICC were treated with $H_2O_2$ in ICC, $H_2O_2$ hyperpolarized the membrane potential under currents clamp mode and decreased both the frequency and amplitude of pacemaker currents and increased the resting currents in outward direction under voltage clamp mode. Also, $H_2O_2$ inhibited the pacemaker currents in a dose-dependent manner. Because the properties of $H_2O_2$ action on pacemaker currents were same as the effects of pinacidil(ATP-sensitive $K^+$ channels opener), we tested the effects of glibenclamide(ATP-sensitive $K^+$ channels blocker) on $H_2O_2$ action in ICC, and found that the effects of $H_2O_2$ on pacemaker currents were blocked by co- or pre- treatment of glibenclamide. These results suggest that $H_2O_2$ inhibits pacemaker currents of ICC by activating ATP-sensitive $K^+$ channels.
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Influence of the SMT2 Knock-out on Hypocotyl Elongation in Arabidopsis thaliana
Hwang, In-Deok,Paudyal, Dilli Prasad,Kim, Seong-Ki,Cheong, Hyeon-Sook Korean Society for Biotechnology and Bioengineerin 2007 Biotechnology and Bioprocess Engineering Vol.12 No.2
Inhibitors are very important in the study of hormone function. Brasinazole (Brz) is a specific inhibitor of brassinosteroids (BRs)biosynthess. To expand our knowledge of the molecular mechanisms of plant steroid signaling, we performed genetic screening using medium containing Brz under dark conditions. Mutants insensitive to Brz develop longer hypocotyls than their wild type counterparts. We isolated abz453 as a Brz insensitive mutant. TAIL-PCR and the segregation ratio of T2 plants indicated a single T-DNA insertion at the 24-Sterol C-methyltransferase (SMT2) gene in the abz453 mutant. Recapitulation for putative FCP serine phosphatase (FSP), the gene neighboring SMT2, indicated no significant phenotypes, but the SMT2 anti-sense (SMT2-AS) line developed longer hypocotyls than the wild type in medium containing Brz. Additionally, the SMT2-AS line displayed similar phenotypes to the abz453 line in soil including enhanced growth and smaller silique. The abz453 and SMT2-AS mutants showed phenotypes similar to those of wild type in medium containing benzylaminopurine, pacrobutrazol and ACC (precursor for ethylene) under dark conditions. However. when brassinolide (BL) dose response was observed, the abz453 and SMT2-AS lines showed higher sensitivity than wild type. The abz453/det2 and abz453/bri1-119 double mutants showed enhanced growth compared to the det2 and bri1-119 line under both dark and light conditions. Specially, in dark conditions double mutants displayed nearly 2- and 1.5-fold longer. hypocotyls than det2 and bri1-119 plants. Brz insensitivity to the SMT2 knock-out mutant and phenotypes of double mutants indicate that not only do BRI1 and DET2 influence the BRs response, as evidenced by hypocotyl elongation, but another sterol derived signals may also be affected in mutants, suggesting that another pathway is involved in hypocotyl elongation due to SMT2.
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