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PARK, SOON DEOK,UH, YOUNG,LEE, GYUSANG,LIM, KWANHUN,KIM, JONG BAE,JEONG, SEOK HOON Blackwell Publishing Ltd 2010 APMIS Vol.118 No.10
<P>Park SD, Uh Y, Lee G, Lim K, Kim JB, Jeong SH. Prevalence and resistance patterns of extended-spectrum and AmpC &bgr;-lactamase in <I>Escherichia coli</I>, <I>Klebsiella pneumoniae</I>, <I>Proteus mirabilis,</I> and <I>Salmonella</I> serovar Stanley in a Korean tertiary hospital. APMIS 2010; 118: 801–8.</P><P>A total of 100 clinical isolates of <I>Escherichia coli</I> (n = 35)<I>, Klebsiella pneumoniae</I> (n = 63)<I>, Proteus mirabilis</I> (n = 1), and Salmonella serovar Stanley (n = 1), showing resistance to cefoxitin, or returning positive in extended-spectrum &bgr;-lactamase (ESBL) by Clinical and Laboratory Standards Institute (CLSI) ESBL confirmatory method, were studied. The isolates were examined by the boronic acid (BA) disk test, polymerase chain reaction, and pulsed-field gel electrophoresis (PFGE) to investigate genetic similarities. The concurrence rates for ESBLs by the CLSI and the BA disk test were 97% for <I>E. coli</I> and 96.7% for <I>K. pneumoniae</I>. A total of 41 isolates showing cefoxitin resistance yielded all positive by the BA disk test. All the 33 <I>K. pneumoniae</I> isolates, which showed positive by the BA disk test, were carrying AmpC genes. The TEM and CTX-M types were predominant in <I>E. coli</I> and the SHV and the CIT and/or DHA types were predominant in <I>K. pneumoniae</I>. PFGE analysis showed almost 75% of genetic similarities among <I>K. pneumoniae</I> isolates producing ESBLs and/or AmpC &bgr;-lactamases (AmpCs) as each <I>K. pneumoniae</I> carried variable genes and showed variable antibiotic patterns. Clearly, the BA disk test was a useful method for the detection of ESBLs and AmpCs. In particular, cefoxitin resistance and BA-positive trait of <I>K. pneumoniae</I> do reflect the presence of AmpC genes in the organism.</P>
The Effect of Propofol on Hypoxic damaged-HaCaT Cells
Park, Chang-Hoon,Kwak, Jin-Won,Park, Bong-Soo,Kim, Yong-Ho,Kim, Yong-Deok,Yoon, Ji-Uk,Yoon, Ji-Young,Kim, Cheul-Hong The Korean Dental Society of Anesthsiology 2014 Journal of Dental Anesthesia and Pain Medicine Vol.14 No.1
Background: Autophagy is a self-eating process that is important for balancing sources of energy at critical times in development and in response stress. Autophagy also plays a protective role in removing clearing damaged intracellular organelles and aggregated proteins as well as eliminating intracellular pathogens. The purpose of the present study was to examine the protective effect of propofol against hypoxic damage using keratinocytes. Methods: Human keratinocytes (HaCaT cells) were obtained from the American Type Culture Collection. Propofol which were made by dissolving them in DMSO were kept frozen at $-4^{\circ}C$ until use. The stock was diluted to their concentration with DMEM when needed. Prior to propofol treatment cells were grown to about 80% confluence and then exposed to propofol at different concentrations (0, 25, 50, 75, $100{\mu}M$) for 2 h pretreatment. Cell viability was measured using a quantitative colorimetric assay with thiazolyl blue tetrazolium bromide (MTT assay), and fluorescence microscopy and western blot analysis were used for evaluation of autophagy processes. Results: The viability of propofol-treated HaCaT cells was increased in a dose-dependent manner. Propofol did not show any significant toxic effect on the HaCaT cells. The autophagy inhibitor, 3-methyladenine, reduced cell viability of hypoxia-injured HaCat cells. Fluorescence microscopy and western blot analysis showed propofol induce autophagy pathway signals. Conclusions: Propofol enhanced viability of hypoxia-injured HaCaT cells and we suggest propofol has cellular protective effects by autophagy signal pathway activation.
Effect of Water Treatment Sludge (WTS) on Cadmium Content in Sorghum (Sorghum bicolor)
Park, Byung-Hoon,Jeong, Sang-Hyeok,Park, Kun-Woo,Yoo, Sung-Mook,Lee, Jae-Deok,Nam, Cha-Young,Park, Mi-Sun,Kim, Young-Han,Kim, Sang-Deog A The Korean Society for Applied Biological Chemistr 2009 Journal of Applied Biological Chemistry (J. Appl. Vol.52 No.3
In this study cadmium content of sorghum hybrid (Sorghum bicolor) was analyzed using atomic absorption spectrophotometer at different burner heights (3, 6, 9 mm). We considered the 6 mm burner height condition favorable among the three. The mean Cd content of each treatment are as follows: Alum+NPK, 1.90 mg/kg; Control, 3.14 mg/kg; Compost, 3.35 mg/kg; and Compost+NPK, 4.23 mg/kg.
박덕훈 ( Deok Hoon Park ),이종성 ( Jong Sung Lee ),정은선 ( Eun Sun Jung ),현창구 ( Chang Gu Hyun ),이지영 ( Ji Young Lee ),허성란 ( Sungran Hur ),고재숙 ( Jae Sook Koh ),이희경 ( Hee Kyung Lee ),백지훈 ( Ji Hwoon Baek ),유병삼 ( 대한화장품학회 2006 대한화장품학회지 Vol.32 No.3
Nucler factor-kappa B (NF-kappaB) 프로모터는 염증성 질환을 유도하는 호염증성 시토카인의 발현에 중요한 역할을 수행하는 전사인자 중의 하나이다. 본 실험에서는 200 여종의 식물추출물들로부터 항염효능이 있는 추출물을 선발하기 위해 NF-kappaB 리포터 실험을 수행하였다. NF-kappaB 리포터 실험결과, 12종의 식물추출물, 즉 개나리, 고추잎, 박하, 뱀딸기, 뽕나무, 삼백초, 솔잎, 양애줄기, 약쑥, 어성초, 왕벚꽃가지, 조릿대 등이 lipopolysaccharide (LPS)에 의해 유도된 NF-kappaB 프로모터 활성을 농도의존적으로 억제하는 것을 확인하였다. 이들 12종의 식물추출물이 호염증성 시토카인 발현에도 동일한 효과를 나타내는지 알아보기 위해 tumor necrosis factor-alpha (TNF alpha)와 인터루킨-8에 대한 ELISA실험을 실시하였다. ELISA실험 결과, NF-kappaB 리포터 실험결과와 동일하게, TNF-alpha와 인터루킨-8 생산이 12종 식물추출물 모두에서 감소됨을 관찰하였다. 이러한 실험결과는, 12종의 식물 추출물에서 보여지는 호염증성 시토카인 억제효과가 NF-kappaB 프로모터 활성억제를 통해 이루어지고 있음을 시사한다. 또한, 이들 12종 식물은 diphenyl-p-picrylhydrazyl (DPPH) assay를 통해 살펴본 결과 높은 항산화 활성도 있음을 확인하였다. 이상의 결과로부터, 12종의 식물 추출물은 염증성 피부질환 전용 화장품 제형에서 항염 및 자극완화 소재로 응용될 수 있음을 확인하였다. Nuclear factor-kappaB (NF-kappaB) is a critical transcription factor for maximal expression of many of the cytokines that are involved in the pathogenesis of inflammatory diseases. In this study, we found that 12 plant extracts among 200 plants, namely, Forsythia koreana, Capsicum annuum L, Mentha arvenis, Duchesnea chrysantha, Morus alba, Saururus Chinenis (Lour) Baill, Pine needle, Zingiber mioga (Thunb.), Roscoe, Houttuynia, Prunus yedoenis, Sasa quelpaertenis, significantly inhibited LPS- induced NF-kappaB activation in a concentration-dependent manner. Additionally, 12 plant extracts were found to have antioxidant activities in DPPH assay Therefore, we have attempted to determine whether 12 herbal extracts could inhibit the expression of cytokines possessing NF-kappaB promoter in their promoter regions. Consistently 12 herbal extracts inhibited LPS-induced production of TNF alpha and interleukin-8 (IL-8). These results show that 12 herbal extracts suppresses the production of pro-inflammatory mediators through the inhibition of the NF-kappaB signaling pathway, we suggest that 12 herbal extracts can be used as a anti-inflammatory and soothing agent.
Tee형상 탄소강 배관 내의 국부감육 현상에 대한 연구
박상훈(Sang Hoon Park),이상규(Sang Kyu Lee),강덕원(Deok Won Kang),황경모(Kyung Mo Hwang),김경훈(Kyung Hoon Kim) 대한기계학회 2010 대한기계학회 춘추학술대회 Vol.2010 No.11
When components made of carbon steel in nuclear, fossil, and industry plants are contacted with flowing fluid, wall thinning caused by FAC can be generated and eventually ruptured at the portion of pressure boundary. Various models have been proposed to predict the wall thinning caused by FAC. The Chexial-Horiwitz model and the Kastner-Riedle model serve as codes to aid utilities in managing pipe degradation. However, existing models cannot predict exactly the local thinning rate and the local wall thinning location. The purpose of this paper is that cause investigation of wall thinning by FAC and prediction exactly the local wall thinning location in Pipe. Furthermore, this study is expanded previous things and intend to draw a conclusion which is capable of application in real field. In this study, Experiments and numerical analysis for three types of piping component, which are used to plants in general, were performed and the results were compared for examination of application of real periodic measurement.