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Yong Ju Jin(Yong Ju Jin),Dawon Jo(Dawon Jo),Soon-Wo Kwon(Soon-Wo Kwon),Samnyu Jee(Samnyu Jee),Jeong-Seon Kim(Jeong-Seon Kim),Jegadeesh Raman(Jegadeesh Raman ),Soo-Jin Kim(Soo-Jin Kim) 한국식물병리학회 2022 Plant Pathology Journal Vol.38 No.6
Pectobacterium odoriferum is the primary causative agent in Kimchi cabbage soft-rot diseases. The pathogenic bacteria Pectobacterium genera are responsible for significant yield losses in crops. However, P. odoriferum shares a vast range of hosts with P. carotovorum, P. versatile, and P. brasiliense, and has similar biochemical, phenotypic, and genetic characteristics to these species. Therefore, it is essential to develop a P. odoriferumspecific diagnostic method for soft-rot disease because of the complicated diagnostic process and management as described above. Therefore, in this study, to select P. odoriferum-specific genes, species-specific genes were selected using the data of the P. odoriferum JK2.1 whole genome and similar bacterial species registered with NCBI. Thereafter, the specificity of the selected gene was tested through blast analysis. We identified novel species-specific genes to detect and quantify targeted P. odoriferum and designed specific primer sets targeting HAD family hydrolases. It was confirmed that the selected primer set formed a specific amplicon of 360 bp only in the DNA of P. odoriferum using 29 Pectobacterium species and related species. Furthermore, the population density of P. odoriferum can be estimated without genomic DNA extraction through SYBR Green-based real-time quantitative PCR using a primer set in plants. As a result, the newly developed diagnostic method enables rapid and accurate diagnosis and continuous monitoring of soft-rot disease in Kimchi cabbage without additional procedures from the plant tissue.
Inseok Jang,Jun-Ho La,Gyu-Tae Kim,Jeong-Soon Lee,Eun-Jin Kim,Eun-Shin Lee,Su-Jeong Kim,Jeong-Min Seo,Sang-Ho Ahn,Jae-Yong Park,Seong-Geun Hong,Dawon Kang,Jaehee Han 대한생리학회-대한약리학회 2008 The Korean Journal of Physiology & Pharmacology Vol.12 No.5
Single-channel recordings of TASK-1 and TASK-3, members of two-pore domain K<sup>+</sup> channel family, have not yet been reported in dorsal root ganglion (DRG) neurons, even though their mRNA and activity in whole-cell currents have been detected in these neurons. Here, we report single-channel kinetics of the TASK-3-like K<sup>+</sup> channel in DRG neurons and up-regulation of TASK-3 mRNA expression in tissues isolated from animals with spinal cord injury (SCI). In DRG neurons, the single-channel conductance of TASK-3-like K<sup>+</sup> channel was 33.0±0.1 pS at −60 mV, and TASK-3 activity fell by 65±5% when the extracellular pH was changed from 7.3 to 6.3, indicating that the DRG K<sup>+</sup> channel is similar to cloned TASK-3 channel. TASK-3 mRNA and protein levels in brain, spinal cord, and DRG were significantly higher in injured animals than in sham-operated ones. These results indicate that TASK-3 channels are expressed and functional in DRG neurons and the expression level is up-regulated following SCI, and suggest that TASK-3 channel could act as a potential background K<sup>+</sup> channel under SCI-induced acidic condition.
Trends in Intraoperative Neuromonitoring for Thyroid Surgery: A Point-Prevalence Survey in Korea
Dawon Park,Young Jun Chai,Hoon Yub Kim,Woong Youn Chung,Kang Dae Lee,Kwang-Yoon Jung,Jeong Soo Kim 대한신경모니터링학회 2021 Journal of Neuromonitoring & Neurophysiology Vol.1 No.1
Background and Objectives To determine the trends in the use of intraoperative recurrent laryngeal nerve (RLN) monitoring during thyroid surgery in Korea. Materials and Methods A web-based anonymous survey was sent to 594 members of the Korean Intraoperative Neural Monitoring Society (KINMoS), who perform thyroid surgeries in Korea. Topics included in the survey covered the demographics, practice setting, surgical volume, access and familiarity with intraoperative neuromonitoring (IONM) technology, use of IONM, perceived advantages or disadvantages, and qualitative and quantitative descriptions of IONM usage. The χ² test was used to examine associations between the use of IONM and dependent variables. Results A total of 78 (13.1%) people completed the survey. Of the respondents, 78.1% answered that they use IONM in at least some thyroid cases: 28% answered that they always use it and 50% used it occasionally. The most common reason for using IONM was because “it is helpful in special cases,” while “cost” was the most common reason for not using IONM. Among the study population, surgeons in tertiary hospitals and those with high surgical volumes used IONM frequently. Surgeons using IONM were often involved in medical education and were more likely to recommend the use of IONM. Statistical correlation was also confirmed between laryngeal examination and IONM utilization. Conclusion IONM is used for thyroid surgeries at a relatively high rate in Korea. Its use is associated with training, hospital setting of the current surgical practice, annual number of surgeries, involvement in medical training/education, familiarity with IONM, and device availability.
Nuclear UPF1 Is Associated with Chromatin for Transcription-Coupled RNA Surveillance
Hong, Dawon,Park, Taeyoung,Jeong, Sunjoo Korean Society for Molecular and Cellular Biology 2019 Molecules and cells Vol.42 No.7
mRNA quality is controlled by multiple RNA surveillance machineries to reduce errors during gene expression processes in eukaryotic cells. Nonsense-mediated mRNA decay (NMD) is a well-characterized mechanism that degrades error-containing transcripts during translation. The ATP-dependent RNA helicase up-frameshift 1 (UPF1) is a key player in NMD that is mostly prevalent in the cytoplasm. However, recent studies on UPF1-RNA interaction suggest more comprehensive roles of UPF1 on diverse forms of target transcripts. Here we used subcellular fractionation and immunofluorescence to understand such complex functions of UPF1. We demonstrated that UPF1 can be localized to the nucleus and predominantly associated with the chromatin. Moreover, we showed that UPF1 associates more strongly with the chromatin when the transcription elongation and translation inhibitors were used. These findings suggest a novel role of UPF1 in transcription elongation-coupled RNA machinery in the chromatin, as well as in translation-coupled NMD in the cytoplasm. Thus, we propose that cytoplasmic UPF1-centric RNA surveillance mechanism could be extended further up to the chromatin-associated UPF1 and co-transcriptional RNA surveillance. Our findings could provide the mechanistic insights on extensive regulatory roles of UPF1 for many cellular RNAs.
Kim, Yongseok,Jeong, Dawon,Min, Hophil,Sung, Changmin,Park, Ju-hyung,Son, Junghyun,Lee, Kang Mi,Kim, Ho Jun,Lee, Jaeick,Kwon, Oh-Seung,Kim, Ki Hun Korean Society for Mass Spectrometry 2017 Mass spectrometry letters Vol.2 No.4
Meldonium is a drug for treating ischemia by expanding the arteries but it can also enhance the performance of sports players. The World Anti-Doping Agency (WADA) has included it in the list of prohibited substances since 2016. Meldonium is one of the challenging substances for anti-doping testing because it is difficult to recover by general liquid-liquid or solid phase extraction due to its permanent charge and high polarity. Therefore, high-performance liquid chromatography (HPLC) is currently used by injecting a diluted urine sample (known as the "dilute-and-shoot" strategy). There is no loss of target compounds in the extraction/cleanup procedure but its high matrix effect could interfere in their separation or detection from the endogenous urinary compounds. We report a single method using high-resolution mass spectrometry that can be used for both screening and confirmation, which follows the "dilute-and-shoot" strategy. In this method, the endogenous compounds' interfering peaks in the mass spectrum are separated at a high resolution of FWHM 140,000, and the results are suitable for substance detection following the WADA guidelines. The interferences in the obtained mass spectrum of the urine matrix are identified as acetylcholine, lysine, and glutamine by further analysis and database searching. Validation of the method is performed in routine anti-doping testing, and the limit of detection is 50 ng/mL. This method uses simple sample preparation and a general reverse phase HPLC column, and it can be easily applied to other substances.
( Yongseok Kim ),( Dawon Jeong ),( Hophil Min ),( Changmin Sung ),( Ju-hyung Park ),( Junghyun Son ),( Kang Mi Lee ),( Ho Jun Kim ),( Jaeick Lee ),( Oh-seung Kwon ),( Ki Hun Kim ) 한국질량분석학회 2017 Mass spectrometry letters Vol.8 No.2
Meldonium is a drug for treating ischemia by expanding the arteries but it can also enhance the performance of sports players. The World Anti-Doping Agency (WADA) has included it in the list of prohibited substances since 2016. Meldonium is one of the challenging substances for anti-doping testing because it is difficult to recover by general liquid-liquid or solid phase extraction due to its permanent charge and high polarity. Therefore, high-performance liquid chromatography (HPLC) is cur-rently used by injecting a diluted urine sample (known as the “dilute-and-shoot” strategy). There is no loss of target compounds in the extraction/cleanup procedure but its high matrix effect could interfere in their separation or detection from the endogenous urinary compounds. We report a single method using high-resolution mass spectrometry that can be used for both screening and confirmation, which follows the “dilute-and-shoot” strategy. In this method, the endogenous compounds` interfering peaks in the mass spectrum are separated at a high resolution of FWHM 140,000, and the results are suitable for substance detection follow-ing the WADA guidelines. The interferences in the obtained mass spectrum of the urine matrix are identified as acetylcholine, lysine, and glutamine by further analysis and database searching. Validation of the method is performed in routine anti-doping testing, and the limit of detection is 50 ng/mL. This method uses simple sample preparation and a general reverse phase HPLC column, and it can be easily applied to other substances.