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위명환,김성오,전원대,어재혁,김진환 한국공업화학회 2004 응용화학 Vol.8 No.2
The DWTSG concept is proposed as a advanced steam generator of liquid metal reactor to avoid sodium-water reaction incidents in the SG. The optimization of steam generator from economic viewpoint is to minimize the total cost of heat transfer area and pumping power. The baffles are primarily used in shell and tube steam generator for inducing cross flow over tube bundles, and as a results, improving heat transfer performance. In this paper a computer based design method, which covers segmentally baffled steam generator is developed for preliminary DWTSG design. And parametric sensitivity studies are done to determine required heat transfer area to meet the specified heat capacity by calculating minimum or allowable shell-side pressure loss.
공침법에 의한 BiDy₂Fe_(5)O_(12) 결정형 나노입자 분말의 제조 및 응용 연구
홍영대,조정환,김태엽,전성욱,홍영명 고신대학교 자연과학연구소 2007 고신대학교 자연과학연구소 논문집 Vol.14 No.-
금속산화물들은 각종 산업재료로서 유용하며 더욱이 그것들의 결정형 나노입자를 경제적으로 제조하는 기술은 21세기 첨단산업에 매우 중요하다. 저자들은 前報에서 화학합성법에 의한 각종 금속산화물의 나노입자를 제조하는 표준공정법을 연구하여 보고하였다. 본 연구에서는 그 표준공정법을 이용하여, 가넷의 일종인 BiDy_(2)Fe_(5)O_(12) 결정 나노입자 분말을 제조하고 그것의 자성체로의 응용을 위해 제품특성을 조사하었다. 가네트 제조과정에 필요한 침전물은 제품설계에 의거 몰비로 계산된 Bi, Dy, Fe의 질산염들의 혼합수용액에 침전제로서 pH NH_(3)-NH_(4)CI 완충용액 일정량을 가하여 얻어졌다. 이렇게 제조된 침전은 열처리과정을 거처 BiDy_(2)Fe_(5)O_(12) 결정형 나노입자 분말로 만들어졌다. 얻어진 제품의 입자크기, 조성, 결정구조 및 광자기성 등 특성시험이 SEM, ICP, XRD, 그리고 VSM 등을 사용하여 실시되었다. 각종 분석법을 사용하여 제품의 특성을 조사한 결과, 최초에 설계한 대로의 자성체가 나노 크기의 분말로 얻어졌으며 이것을 통하여 전보에서 확립한 표준공정법의 유효성도 입증되었다. Metallic oxides are useful as various industrial materials. Futhermore, the cost-effective techniques of fabricating the crystalline nanopartides of metallic oxides are very important for the high-tech industries of the 21 century, In the previous study, the authors reported the standard process for the preparation of crystalline nanoparticles of metallic oxides by chemical synthesis, In this study, BiDyzFesOiz nanoparticles were prepared by using the reported standard process and the characteristics of the product were investigated for applicability as magnetic substance. The precipitation needed for manufacturing process of garnet was made by adding the NH_(3)-NH_(4)CI buffer solution of pH 10 to the mixed solution of bismuth (III) nitrate, dysprosium(III) nitrate and iron(III) nitrate. The amount of each nitrate was calculated by the designed mole ratios. The nano-size crystalline powders of BiDy_(2)Fe_(5)O_(12) were earned by heating the precipitation at 800℃ for 4 hours in air. The characterizations, such as particle size, composition, crystal structure and magneto-optical property of garnet were tested by SEM, ICP, XRD, and VSM analyses. The results of the analyses showed the following two facts. First, a good magnetic substance of BiDy_(2)Fe_(5)O_(12) was earned as crystalline nano-size powders according to the original design. Next, the established standard process for the fabrication of METALLIC oxides nanonartieles was approved as a very effective method.
Large Volume Sample Stacking 방법과 Head Column Stacking 을 이용한 프로틴과 펩타이드의 시료 농축
강대천,도길명,김용성,정두수 경남대학교 기초과학연구소 2001 硏究論文集 Vol.15 No.-
현재 세포 성분 내에서의 단백질들의 기능규명, 새로운 신약의 개발 등은 매우 중요함을 차지하고 있다. 그러나 현재의 분석 기술인 2-D 젤 모세관 전기 영동 장치, 액체 크로마토그래피 그리고 질량 분석기 등의 여러 가지 방법들은 세포 내에서 극 미량의 단백질들을 분석하는데 있어서 충분한 감도를 제공하지 못한다. 이 실험에서는 모세관 전기 영동 장치를 이용한 단백질과 펩타이드의 분석에서 시료의 낮은 농도의 감도를 향상시키기 위하여 시료 농축 방법인 Large Volume Sample Stacking 방법과 Head column stacking 방법을 이용하여 분석을 하였다. α - lactalbumin과 trypsin inhibitor들과 같은 단백질들을 위의 시료 농축 방법을 이용하여 낮은 시료의 농도 0.01 ㎍/㎖까지 농축이 가능하였다. 또한 효소로 처리한 잘려진 단백질들의 절편들도 극 미량의 low femtomole 수준까지 검출이 가능하였다. 그리고 low femtomole 수준의 단백질 절편들을 규명하기 위하여 CE-MS를 사용하였다 The identification proteins in the cell is very important for Proteomics research. However, current analytical techniques such as 2-D gel electrophoresis, 1iquid chromatography and mass spectrometry do not provide enough sensitivity for cell proteins especially for low-abundant proteins. Therefore, protein and peptide concentration method should be developed for the easy operation of current identification technologies. In order to enhance the concentration sensitivity in capillary electrophoresis of proteins deptides, large volume sample stacking using electroosmotic flow pump and LVSEP combined with Head column stacking were applied. Proteins such as α -Lactalbumin and trypsin inhibitor were successfully stacked and their limits of detection were below 0.01㎍/㎖. A large volume of digested protein samples was also stacked and the enhanced sensitivity allowed one to analyze protein digests at low femtomole levels. CE-MS was employed for the identification of protein digests at low femtomole levels.
Poster Session : PS 0849 ; Upper GI Tract : Ectopic Sebaceous Glands in Esophagus
( Dae Seong Myung ),( Young Eun Joo ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
The occurrence of ectopic sebaceous glands is not unique to humans. Ectopic sebaceous glands have been found in various tissues, such as the lips and mouth, external genitalia, parotid glands, eyelashes, and lacrimal caruncle, palms and soles, and tongue. In humans, this lesion is mostly found in the buccal cavity, urogenital tract, and various organs. Lesions that are located on the lips and in the mouth are referred to as Fordyce`s spots or Fordyce`s disease. The presence of ectopic sebaceous glands in the esophagus was fi rst reported by De La Pava and Pickren in 1962, but there is very little data on this condition. Only a few cases have been reported. Here, we present an interesting and rare case involving tiny lesions scattered throughout the whole esophagus. An asymptomatic 56-year-old man visited our hospital for screening endoscopic examination. He had no previous history of gastroesophageal refl ux disease, hyperlipidemia, smoking and alcohol ingestion. Laboratory examinations were within normal range. Endoscopic examination showed numerous variable sized, slightly elevated yellowish granular lesions, scattered throughout the entire esophagus (fi gure A). Biopsy from yellowish granular lesions was performed. Histopathological examination revealed the lobules of cells showing microvesicular vacuolization and sebaceous cell differentiation in the lamina propria. There were no abnormalities of overlying squamous epithelium. The diagnosis was compatible with those of ectopic sebaceous glands. He received the annual endoscopic examination without treatment. The endoscopic follow-up examination after 7 years showed the slight increase in number or size of lesions (fi gure B). He was still asymptomatic.
( Dae Seong Myung ),( Young Lan Park ),( So Young Joo ),( Eun Myung ),( Cho Yun Chung ),( Hyung Chul Park ),( Jong Sun Kim ),( Sung Bum Cho ),( Wan Sik Lee ),( Hyun Soo Kim ),( Young Eun Joo ) 대한장연구학회 2013 Intestinal Research Vol.11 No.4
Background/Aims: Epigallocatechin-3-gallate (EGCG) is the main polyphenol in green tea and has anti-inflammatory and anti-oxidative effects. The aim of this study was to determine the impact of EGCG on the expression of adhesion molecules and lipopolysaccharide (LPS)-induced nuclear factor-kappa B (NF-κB) signaling in rat intestinal epithelial (RIE) cells. Methods: The effect of EGCG on LPS-induced NF-κB signaling and expression of intercellular adhesion molecule (ICAM)-1 and vascular cell adhesion molecule (VCAM)-1 was examined by reverse transcription polymerase chain reaction, western blotting, immunofluorescence and electrophoretic mobility shift assay. Results: LPS-induced expression of ICAM-1 and VCAM-1 mRNA was inhibited by EGCG treatment in RIE cells. LPS-induced inhibitor of kappa B alpha degradation and NF-κB nuclear translocation were blocked by EGCG in RIE cells. EGCG blocked LPS-induced NF-κB DNA-binding activity in RIE cells. The pharmacological NF-κB inhibitor Bay11-7082 suppressed the LPS-induced expression of ICAM-1 and VCAM-1 mRNA in RIE cells. Conclusions: These results indicate that EGCG inhibits LPS-induced ICAM-1 and VCAM-1 expression by blocking NF-κB signaling in intestinal epithelial cells. (Intest Res 2013;11:261-267)
Dae Seong Choi,Dong Yong Kim,Hee Sun Yang,Yong Dae Park,Chang Hyun Jin,육홍선,Myung Woo Byun,Il Yun Jeong 한국식품과학회 2010 Food Science and Biotechnology Vol.19 No.4
Possibility of photostimulated luminescence (PSL) and electron spin resonance (ESR) to detect γ-irradiation of jujube (Zizyphus jujuba) was investigated. The jujube samples were exposed to γ-irradiation at doses of 1, 3, 5, and 10 kGy. The PSL results showed that jujube exposed to at 1 kGy or more had a photon count of greater than 5,000 count/60 sec (positive), while the non-irradiated jujube yielded a photon count of less than 700 photon count/60 sec (negative). However, physical parameters (light exposure, storage, and thermal treatment) of irradiated jujube samples gave a strong effect on the PSL signal,resulting in decrease of the photon counts from the initial counts. ESR spectroscopy analysis of irradiated jujube revealed specific signals derived from cellulose radicals,and irradiation caused a significant increase in the ESR signal intensity of the jujube samples without any changes in the spectral patterns. Light exposure, storage, and thermal treatment of jujube resulted in significant decreases in the ESR signal intensities of cellulose radicals.