http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
이길용,임건한,소영석,노준,김철성,장대수 朝鮮大學校 附設 醫學硏究所 2002 The Medical Journal of Chosun University Vol.27 No.2
The co-existence of mouth and genital ulceration with iritis was first defined as a syndrome by Behcet. It is now known to be a chronic and relapsing systemic disease, which involves many other systems. Neurogenic bladder dysfunction due to Behcet's disease, however, is extremely rare, and only a limited number of cases have been reported in the international literature. Voiding disfunction in Behcet's disease can be due to neurological or direct bladder involvement and can be related to bladder and sphmctehc components in both phases of micturition. We report on a 35-year old patient with a neuropathic bladder secondary to Behcet's disease.
항진균성 항생물질을 생산하는 Streptoverticillium sp. NA-4083의 분리 및 동정
임대석,윤상균,이명섭,윤원호,김창한 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.6
전국 각지의 토양에서 441균주의 방선균을 분리한 결과 Trichophyton mentagrophytes, Trichophyton rubrum, Nanniza otae 및 Pyricularia oryzae 등의 진균에 광범위한 항진균활성을 나타내는 방선균 NA-4803을 선발하여 그 균주의 동정을 실시하였다. 분리균주 NA-4803은 Gram 양성세균 4균주, Gram 음성세균 2균주, 효모 2균주, 곰팡이 5균주, 조류 1균주에 대해 약한 항균활성을 보이는 반면, 곰팡이 Trichophyton mentagrophytes, Trichophyton rubrum, Nanniza otae 및 Pyricularia oryzae에 대해서는 비교적 강한 항진균활성을 나타내었다. 분리균주 NA-4803은 생리적 특성, 항균활성, lectithinase 및 lipolytic 활성, 각종 유기물의 분해력, 항생물질에 대한 저항성, 온도, pH 및 화학저해제의 첨가에 따른 생육, 질소원의 이용성 및 탄소원의 이용성 등에 있어서 cluster group F에 속하며 Cluster No. 58에 해당하는 Streptoverticillium blastmyceticum과 가장 유사하게 비교·동정되었으나 nitrate 환원성의 양성반응, L-tyrosine 분해력의 음성반응 및 cephaloridine에 대한 저항성에 음성반응을 보였고, 탄소원 중 L-rhamnose와 inulin을 이용하는 것으로 보아 Streptoverticillium blastmyceticum과 다른 특성을 나타내었다. 따라서 분리균주 NA-4803은 Streptoverticillium sp. NA-4803으로 동정되었다. The aim of the present research program was to develop a strain of actinomycetes producing antifungal substance. Soil samples were collected from various sites in Korea and a number of actinomycetes were isolated from the soil samples by applying selective agar for actinomycetes. Among over 440 isolates, a strain (NA-4803) producing antifungal substance against Trichophyton spp. Nannizzia otae and Pyricularia oryzae was selected. The strain NA-4803 was identified as strain similar to Streptoverticillium blastmyceticum with respect to morphological and physiological characteristics, lecithinase and lipolytic activity, degradation of organic compounds, resistance to antibiotics and utilization of carbon and nitrogen sources. But it showed some differences such as positive reaction of nitrate reduction, negative reaction of L-tyrosine degradation, resistance to cephaloridine, and utilization of I rhamnose and inulin. The strain NA-4803 was named as Streptoverticillium sp. NA-4803.
항 Vancomycin 내성균성 항생물질을 생산하는 Streptomyces sp.AMLK-335의 배양조건
이기형,임대석,최경희,이경호,김창한,김정환 건국대학교 동물자원연구센터 2000 動物資源硏究誌 Vol.21 No.-
본 연구는 새로운 항 vancomycin 내성균성 항생물질을 개발하기 위하여 실시되었으며, 전국 각지의 토양 시료로부터 분리한 방선균 중 vancomycin 내성균주인 K-98 Enterococcus faecium 642, K-98 Enterococcus faecium 637 균주에 대하여 항생물질을 생산하는 분리균주 AMLK-335를 선정하여 이 균주의 항생물질 생산에 대한 최적 발효 조건을 검토한 결과, 탄소원 첨가로는 glycerol(0.5%), 질소원 첨가로는 yeast extract(0.5%)가 가장 효과적으로 항생물질 생산에 이용되었고, 비타민을 첨가에 따른 항생물질 생산성의 차이는 크게 나타나지 않았으나, calcium pantothenate 첨가시 항균활성이 비교적 높아졌으며, 금속이온의 첨가는 K+, Pb+, Ba²+, NA²+ , Ca²+를 첨가하였을 때는 항생물질의 생산성이 비교적 높아졌으며, 그중 Ca²+ 첨가구가 가장 생산성이 높았으며, Fe²+ , Hg+의 금속이온을 첨가하였을 때는 항생물질 생산성이 감소하였으며, CaCO₃첨가는 비첨가구보다 높은 항생물질 생산성을 보였으나, 첨가량에 따른 영향은 대체적으로 없었다. pH의 변화에 따른 생산성은 전반적으로 pH가 항생 물질 생산에 영향을 미치지 못하였으나, pH 4.5∼6.0 부근에서는 비교적 높은 항생물질 생산성을보였으며, 통기량에 따른 항생물질 생산성은 통기량이 많을수록 항생물질 생산이 좋았다. 한편 배양기간에 따라 항생물질 생산성을 조사한 결과, 5일째에 가장 높은 항생물질 생산성을 나타내었으며, pH의 변화는 배양 초기에 하강하였다가 점차 상승하는 경향을 보였다. This study was carried out for examining antibiotic productivity of the strain Streptomyces sp. AMLK-335 producing anti-VRE antibiotic. According to different condition, the strain was cultured for 4 days at 27℃ and determined cell growth, pH value and antibiotic productivity using disk diffusion method. In the experiment, the strain Streptomyces sp. AMLK-335 was examined various culture conditions which was added 8 carbon sources, 12 nitrogen sources, 8 vitamins and 11 metal ions in each time and controlled concentration of CaCO₃, initial pH and aeration. From the studies of conditions for antibiotic production, antibiotic productivity was the best to use 0.5% glycerol as carbon source and 0.5% yeast extract as nitrogen source. When several vitamins were added to medium, there was little difference in antibiotic productivity. But the addition of calcium pantothenate slightly increased antimicrobial activity. The addition of metal ion, such as K+, Pb+, Ba²+, NA²+ and Ca²+ slightly increased antibiotic productivity except Fe²+ and Hg+. The addition of CaCo₃showed higher than non-addition experiment in antibiotic production, but there wasn't generally influenced at addition concentration. According to pH change, antibiotic productivity was increased at pH 4.5∼6.0, and when there was a lot of aeration, antibiotic productivity was increased.
Adenovirus-Mediated p53 Treatment Enhances Photodynamic Antitumor Response
Lim, Dae-Seog,Bae, Su-Mi,Kwak, Sun-Young,Park, Eun-Kyung,Kim, Jong-Ki,Han, Sei-Jun,Oh, Chung-Hun,Lee, Chang-Hee,Lee, Won-Young,Ahn, Woong-Shick Mary Ann Liebert 2006 Human gene therapy Vol.17 No.3
<P>Photodynamic therapy (PDT) has been reported to be effective for treating various tumors and to induce apoptosis in many tumor cells. In this study, we evaluated the ability of PDT combined with a tumor suppressor factor, recombinant adenovirus p53 (AdCMVp53), to induce apoptosis as well as cell growth inhibition in CaSki human cervical cancer cells and in nude mice with implanted CaSki cells. To examine levels of apoptosis, CaSki cells were treated with PDT and/or AdCMVp53, and an annexin V-staining assay was then conducted. In addition, Western blot analysis was done to identify p53 induction at the cellular and tumor tissue levels. PDT+AdCMVp53 cotreatment caused remarkable inhibition of CaSki cell proliferation, as compared with the individual treatments. In parallel with the inhibition of cell proliferation, the cotreatment caused a significantly greater increase in the annexin V-stained cell population compared with the individual treatments, as determined by fluorescence-activated cell-sorting analysis. The Western blotting assay also showed significantly more cellular p53 expressed after PDT+AdCMVp53 cotreatment than after each separate treatment. This was consistent with observations of tumor tissue in the mouse system. However, apoptosis- related protein, p21, was significantly suppressed by PDT+AdCMVp53 cotreatment, contrary to treatment with AdCMVp53 alone. Taken together, these findings suggest that PDT plus AdCMVp53 gene therapy exerts more potent antitumor effects on human cervical cancer cells, with induction of apoptosis at least through activation in p53 protein at the cellular and tumor tissue levels.</P>
( Dae Won Jun ),( Jun Young Song ),( Woo Seog Kim ),( Sung Gye Lee ),( Jong Sin Lee ),( Eun Kyung Baek ),( Ki San Joo ),( Myung Sook Yoon ),( Young Lim ),( Yook Kim ),( Il Kwon Park ),( Young Cheol Ju 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1
Aims: Previous reports demonstrated that carnitine restore mitochondrial and liver function in various liver diseases. We aimed to assess various metabolic profiles after 3 months carnitine-orotate complex (Godex<sup>Ⓡ</sup>) treatment in patients with metabolic disease. Methods: We reviewed the records of 544 outpatients (type 2 diabetes, dyslipidemia, and hypertension) from 31 primary or secondary care clinics from January 2015 to May 2016 in Korea. The key inclusion criteria were such as following; i) participants who were prescribed antidiabetic, antihypertension, or lipid-lowering drugs, ii) elevated alanine aminotransferase activity more than 40 IU/L, iii) who were newly prescribed carnitine-orotate complex. Biochemical parameters (liver enzyme, glycated hemoglobin, lipid profile) as well as anthropometric markers observed for 3 months. Results: There was no significant weight change between before and after treatment with carnitine-orotate complex (71.55±12.19 kg vs. 71.1±11.8 kg, P=0.5367). After 3 months of combined treatment with carnitine-orotate complex, the rates of normalization of serum ALT and AST levels were 68.2 % and 63.2%, respectively. Mean change in serum ALT levels from before at 3 months was -44.07±36.15 IU/L (P<0.0001) while it was -33.28±39.46 IU/L in serum AST levels (P<0.0001). Carnitine-orotate complex showed improvement in HbA1c level with 0.74 % decrease (P<0.0001) in diabetes. Serum triglyceride level significantly decreased 54.22 mg/dl compare to baseline in dyslipidemic subjects (P<0.0001). Systolic and diastolic blood pressure also significantly decrease in hypertensive subjects (-6.84 mmHg vs. -3.86 mmHg, respectively, P<0.0001). Conclusions: Three months of treatment with carnitine-orotate complex effectively decreased liver enzyme in patients with various metabolic diseases.
( Dae-woon Jeong ),( Won-bi Han ),( Jae-oh Shim ),( Won-jun Jang ),( Yeol-lim Lee ),( Hyun-seog Roh ),( Jae Hoi Gu ),( Yong Taek Lim ) 한국폐기물자원순환학회(구 한국폐기물학회) 2014 한국폐기물자원순환학회 심포지움 Vol.2014 No.1
A rapid one step direct synthesis of high temperature water gas shift (WGS) catalysts, magnetite incorporated with various promoters like Co, Ni, Cu, and Zn have been shown. An amino acid glycine was used as a complexing agent as well as a fuel for the auto-combustion process. The zwitterionic nature of glycine favors the easy incorporation of the promoter metal ions. In addition, reducing atmosphere created by glycine during its burning makes it possible to prepare magnetite directly. During the high temperature WGS, the activity follows the order Cu-FAG > Ni-FAG > Zn-FAG > Co-FAG. The optimization of Cu loading is also carried out. At 12.5 mole % of Cu loading the catalytic activity reached a CO conversion value of 87% at 400℃.