민어(Miichthys miiuy)의 卵發生過程 및 仔稚魚 발육단계에 따른 형태발달

한경호,오성현,황동식,조영현,서대철 한국어류학회 2002 韓國魚類學會誌 Vol.14 No.2

본 연구는 2000년 8월 19일 전라남도 신안군 임자도 연안에서 2각망으로 어획된 민어 어미를 인위적인 방법으로 수정하여 부화 사육하면서 관찰한 卵發生 過程 및 仔稚魚 형태변화는 다음과 같다. 1. 민어의 受精卵은 구형의 분리 부성난으로 알의 크기는 0.96~1.08㎜(평균 1.01㎜, n=50)였고, 크고 작은 油球가 1~5개(0.06~0.34㎜) 존재하였다. 2. 사육수온 25.2~28.4℃에서 受精 후 17시간 10분에는 배체가 卵膜을 뚫고 머리부터 孵化하기 시작하였다. 3. 孵化 직후 仔魚의 전장은 2.36~2.63㎜(평균 2.48㎜, n=10)로 전장의 45.0~47.0%에 달하는 큰 卵黃(장경 1.11~1.20㎜, 단경 0.92~0.96㎜)을 가지고 있으며 筋節은 7~8+ 17~19=24~27개였다. 4. 孵化 후 3일째 仔魚는 전장 3.98~4.32㎜(평균 4.16㎜, n=10)로 입은 열려있었고, 이 시기에 섭이활동이 활발하게 관찰되면서 卵黃이 완전히 흡수되어 後期仔魚期로 이행되었다. 5. 孵化 후 10일째 後期仔魚는 전장이 5.88~6.74㎜(평균 6.30㎜, n=10)로 꼬리의 말단이 굽어지기 시작하는 시기로 黑色素胞가 기저후두골 뒷부분의 체측에서 점모양 및 나뭇가지모양 으로 출현하였다. 6. 孵化 후 31일째 개체의 전장은 9.62~12.76㎜(평균 11.12㎜, n=10)로 모든 지느러미가 정수에 달하였고, 체형이나 반문이 成魚와 닮아 種魚期로 이행하였다. Egg development and morphological change of larvae of the brown croaker, Miichthys miiuy, were investigated after obtaining samples by artificial insemination. The spawning season extends from the middle to the end of August in the seas off Lim-ja Island, Cheollanam-do. The fertilized eggs were transparent, spherical, separate and they floated. Egg diameter ranged 0.96~1.08㎜ with several oil globules of 0.06~0.34㎜. Hatching occurred 17 hours 10 minutes after fertilization in 25.2~28.4℃ water temperature. The newly-hatched larvae were 2.36~2.63㎜(mean:2.48㎜, n=10) in total length(TL), had a large yolk, and 7~8+17~19=24~27 myotomes. Melanophores were distributed on the eye lens. Three days after hatching the larvae had attained 3.98~4.32㎜ in TL(mean:4.16㎜, n=10), the yolk sac was completely absorbed, and they had transformed into the postlarva stage. The larva reached the juvenile stage with all fins formed and with a complete set of fin rays(D.Ⅹ-28~31;A.Ⅱ-7~8;P. 21~23;C. 19~21;V. 25~26) on the 31st day after hatching. The juveniles were 9.62~12.76㎜(mean:11.12㎜, n=10) in total length. At this point the juvenile was similar to the adult in body form and pigmentation pattern.

톡소포자충의 세포질 항원 및 세포막 항원의 면역학적 특성 분석

이영하,한경민,김계영,신대환 충남대학교 의과대학 지역사회의학연구소 1995 충남의대잡지 Vol.22 No.2

The aim of this study was to evaluate the differences in humoral immune response between cytoplasmic soluble antigen and membrane antigen of Toxoplasma gondii. The cytoplasmic soluble and membrane antigens were prepared from T gondii tachyzoites by means of ultrasonication and treatment of Triton X-100. Sera were collected from mice immunized with each antigen at weekly interval for 10 weeks. The serum antibodies were detected by ELISA, and the antidody-binding proteins were analyzed by Western blot. The serum IgM titers were increased in mice immunized with cytoplasmic soluble antigen from 3 weeks, and the titers were peaked between 6 and 8 weeks. However the IgM antibody titers from mice immunized with the membrane antigen were not significantly increased in comparison with negative control group. The serum IgG titers were not revealed significant differences bewteen groups of antigens. Sera from mice immunized with cytoplasmic soluble antigen and membrane antigens reacted with specific bands of T.gondii from 2 and 3 weeks after immunization, respectively. During the course of immunization additional bands appeared consecutively, and antibody-binding antigens were reacted mainly with the molecular weight from 22-kDa to 68-kDa after 5 weeks. In conclusion, there were not distinguished specific humoral immune responses between cytoplasmic soluble antigen and membrane antigen of T.gondii, except serum IgM antibody responses.

항 Vancomycin 내성균성 항생물질을 생산하는 Streptomyces sp.AMLK-335의 배양조건

이기형,임대석,최경희,이경호,김창한,김정환 건국대학교 동물자원연구센터 2000 動物資源硏究誌 Vol.21 No.-

본 연구는 새로운 항 vancomycin 내성균성 항생물질을 개발하기 위하여 실시되었으며, 전국 각지의 토양 시료로부터 분리한 방선균 중 vancomycin 내성균주인 K-98 Enterococcus faecium 642, K-98 Enterococcus faecium 637 균주에 대하여 항생물질을 생산하는 분리균주 AMLK-335를 선정하여 이 균주의 항생물질 생산에 대한 최적 발효 조건을 검토한 결과, 탄소원 첨가로는 glycerol(0.5%), 질소원 첨가로는 yeast extract(0.5%)가 가장 효과적으로 항생물질 생산에 이용되었고, 비타민을 첨가에 따른 항생물질 생산성의 차이는 크게 나타나지 않았으나, calcium pantothenate 첨가시 항균활성이 비교적 높아졌으며, 금속이온의 첨가는 K+, Pb+, Ba²+, NA²+ , Ca²+를 첨가하였을 때는 항생물질의 생산성이 비교적 높아졌으며, 그중 Ca²+ 첨가구가 가장 생산성이 높았으며, Fe²+ , Hg+의 금속이온을 첨가하였을 때는 항생물질 생산성이 감소하였으며, CaCO₃첨가는 비첨가구보다 높은 항생물질 생산성을 보였으나, 첨가량에 따른 영향은 대체적으로 없었다. pH의 변화에 따른 생산성은 전반적으로 pH가 항생 물질 생산에 영향을 미치지 못하였으나, pH 4.5∼6.0 부근에서는 비교적 높은 항생물질 생산성을보였으며, 통기량에 따른 항생물질 생산성은 통기량이 많을수록 항생물질 생산이 좋았다. 한편 배양기간에 따라 항생물질 생산성을 조사한 결과, 5일째에 가장 높은 항생물질 생산성을 나타내었으며, pH의 변화는 배양 초기에 하강하였다가 점차 상승하는 경향을 보였다. This study was carried out for examining antibiotic productivity of the strain Streptomyces sp. AMLK-335 producing anti-VRE antibiotic. According to different condition, the strain was cultured for 4 days at 27℃ and determined cell growth, pH value and antibiotic productivity using disk diffusion method. In the experiment, the strain Streptomyces sp. AMLK-335 was examined various culture conditions which was added 8 carbon sources, 12 nitrogen sources, 8 vitamins and 11 metal ions in each time and controlled concentration of CaCO₃, initial pH and aeration. From the studies of conditions for antibiotic production, antibiotic productivity was the best to use 0.5% glycerol as carbon source and 0.5% yeast extract as nitrogen source. When several vitamins were added to medium, there was little difference in antibiotic productivity. But the addition of calcium pantothenate slightly increased antimicrobial activity. The addition of metal ion, such as K+, Pb+, Ba²+, NA²+ and Ca²+ slightly increased antibiotic productivity except Fe²+ and Hg+. The addition of CaCo₃showed higher than non-addition experiment in antibiotic production, but there wasn't generally influenced at addition concentration. According to pH change, antibiotic productivity was increased at pH 4.5∼6.0, and when there was a lot of aeration, antibiotic productivity was increased.

Distributed memory access architecture and control for fully disaggregated datacenter network

Kyeong-Eun Han(Kyeong-Eun Han),Ji Wook Youn(Ji Wook Youn),Jongtae Song(Jongtae Song),Dae-Ub Kim(Dae-Ub Kim),Joon Ki Lee(Joon Ki Lee) 한국전자통신연구원 2022 ETRI Journal Vol.44 No.6

In this paper, we propose novel disaggregated memory module (dMM) architecture and memory access control schemes to solve the collision and contention problems of memory disaggregation, reducing the average memory access time to less than 1 μs. In the schemes, the distributed scheduler in each dMM determines the order of memory read/write access based on delay-sensitive priority requests in the disaggregated memory access frame (dMAF). We used the memory-intensive first (MIF) algorithm and priority-based MIF (p-MIF) algorithm that prioritize delay-sensitive and/or memory-intensive (MI) traffic over CPU-intensive (CI) traffic. We evaluated the performance of the proposed schemes through simulation using OPNET and hardware implementation. Our results showed that when the offered load was below 0.7 and the payload of dMAF was 256 bytes, the average round trip time (RTT) was the lowest, ~0.676 μs. The dMM scheduling algorithms, MIF and p-MIF, achieved delay less than 1 μs for all MI traffic with less than 10% of transmission overhead.

Effects of dietary diglycerides as fish oil replacer on the growth performance of olive flounder, Paralichthys olivaceus

Dae-Han Oh,Kwan-Sik Yun,Kang-Woong Kim,Kyoung-Duck Kim,Bong-Joo Lee,Sang-Woo Hur,Hyon-Sob Han,Kyeong-Jun Lee 한국수산해양기술학회(구 한국어업기술학회) 2017 한국수산해양기술학회 학술발표대회 Vol.2017 No.11

배합사료의 크기, 공급횟수 그리고 사육밀도가 조피볼락 (Sebastes schlegelii) 치어의 성장에 미치는 영향

오대한 ( Dae Han Oh ),송진우 ( Jin Woo Song ),김민기 ( Min Gi Kim ),이봉주 ( Bong Joo Lee ),김강웅 ( Kang Woong Kim ),한현섭 ( Hyon Sob Han ),이경준 ( Kyeong Jun Lee ) 한국수산과학회 2013 한국수산과학회지 Vol.46 No.4

We performed four sets of feeding trials to establish optimum feed size (Exp-I), stocking density (Exp-II) and feeding frequency (Exp-III and IV) for Korean rockfish. In Exp-I, three different particle sizes of a commercial diet (small, 2.0-2.1 mm; medium, 2.4-3.2 mm; and large, 4.0-5.3 mm) were fed to four replicate groups of fish (22.8±0.1g), each of which was fed to apparent satiation for six weeks. In Exp-II, fish (44.3±0.4 g) were reared at four stocking densities (1.5, 3.0, 4.5 and 6.0 kg/m3) and fed a commercial diet to apparent satiation for four weeks. In Exp-III, fish (14.8±0.1 g) were fed a commercial diet once, twice, thrice, 4× or 5× a day at a feeding ratio of 3.25% of body mass. Another group of fish was fed the same commercial diet 5× a day to apparent satiation; this treatment was designated FS (five times satiation/day). In Exp-IV, fish (31.3±0.1 g) were fed a commercial diet once, twice, thrice or 4× a day to apparent satiation. Another group of fish was fed to apparent satiation once every 48 hours. In Exp-I, fish fed the large particle (4.0-5.3 mm) diet had a significantly higher feed conversion ratio and lower protein efficiency ratio than fish fed the small particle diet. In Exp-II, groups of fish reared at densities of 4.5 and 6.0 kg/m3 had significantly higher feed intake and growth performance than fish reared at 1.5 and 3.0 kg/m3. In Exp-III, fish fed to apparent satiation had significantly higher growth performances than fish fed once or 4× a day. A significantly higher feed conversion ratio and a lower protein efficiency ratio were obtained in the FS group. In Exp-IV, growth performance and feed utilization efficiency were not significantly affected by experimental variation in feeding frequency. Fish fed to apparent satiation once every 48 hours had better feed utilization than those in other treatments and growth performances of those were comparable. Therefore, the optimum feed particle size, stocking density and feeding frequency for Korean rockfish under conditions we used were 2.0-3.2 mm, 4.5-6.0 kg/m3, and once a day or once every 48 hour, respectively.

Force Adjustment of an Active Pipe Inspection Robot

Dae-Won Kim,Chul-Han Park,Hak-Kyeong Kim,Sang-Bong Kim 제어로봇시스템학회 2009 제어로봇시스템학회 국제학술대회 논문집 Vol.2009 No.8

This paper proposes an active pipe inspection robot with the wall-pressing force adjustment using DC motor.It is developed for long distance inspection of sea-water pipelines such as horizontal linear pipelines and slope linearpipelines with large variable diameters from 600mm to 800mm. Its mechanical design consists of two modules asdriving module and control module. The driving module has three pantograph type links spaced in 120° with threecaterpillar track wheels. This design makes it possible to realize the adaptation to pipe diameter and the adjustment ofwall-pressing force. The control module consists of a micro controller, motor driver and sensor interface. An active pipeinspection robot is designed by 3D machine design program. To control the pipe inspection robot, firstly, the pipeinspection robot is considered as a mass-spring-damper system. The dynamic model of the system is obtained byprocess model identification method. Secondly, an observer is designed to estimate the unknown wall-pressing force tosustain the pipe inspection robot in pipeline. Thirdly, an algorithm of wall-pressing force generator is presented to findout an appropriate wall-pressing force, and the presented appropriate wall-pressing force is given as a reference value ofwall-pressing force. Fourthly, PD controller is designed to make the estimated wall-pressing force track the referencewall-pressing force irrelatively to variable diameter of pipeline. Finally, the driving tests of the pipe inspection robot areperformed in horizontal pipelines and slope pipelines of 30°. The test results are shown to prove the effectiveness of thedeveloped pipe inspection robot.

Isolation and Characterization of Immunomodulatory Glycoprotein from the Root of Panax ginseng

Han Jae Shin,Kyeong Mee Park,Young Sook Kim,Ki Yeul Nam,You Hui Lee,Jong Dae Park 고려인삼학회 2000 Journal of Ginseng Research Vol.24 No.3

[P-24] More Insights Gained in Parrot Fish, Oplegnathusfasciatus Fed The Diet Containing Cheongkukjang

Dae-Han Oh,Minh-Anh Pham,Kyeong-Jun Lee 한국수산과학회 양식분과 2011 한국수산과학회 양식분과 학술대회 Vol.2011 No.6

기관지천식의 중증도에 따른 안지오테신 전환효서 유전자 다형에 관한 연구

한선애,이신형,심재정,유세화,조재연,차대룡,이상엽,강세용,강경호,인광호 대한알레르기학회 1998 천식 및 알레르기 Vol.18 No.4

Background: The angiotensin-converting enzyme (ACE) has a major role in the degradation of bradykinin, tachykinin, substance P which are associated with bronchial hyperresponsiveness and inflammation. The other role of ACE is the genesis of angiotensin II which causes bronchial smooth muscle contraction. The deletion polymorphism of ACE gene(DDtype) may be related to the high serum level of ACE. Objective: We studied to evaluate an association between the insertion /deletion polymorphism of the ACE gene and asthma, and its severity. Materials and methods: Sixty asthmatic patients and 44 healthy controls were enrolled. Severity of asthma was classified by the guideline of NHLBI/WHO workshop. The ACE genotypes of all the subjects were determined by polymerase chain reaction. Results: The distribution of ACE genotypes were not significantly different between healthy controls and asthma group (p)0.05). In asthmatic patients, the genetic polymorphism was similar between different severity groups (p) 0.05). Conchcsion: It is suggested that I/D polymorphism of the ACE gene may not be associated with development of asthma. The severity of asthma may not be influenced by I/D polymorphism of the ACE gene.