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Identification and expression analysis of grape LRK10L-2 genes during grape fruit development
Ma Jin-Ping,Yin Xue-Ren,Wei Tong-Lu,Liu Hai-Nan,Pei Mao-Song,Yang Sheng-Di,Jin Hui-Ying,He Guang-Qi,Guo Da-Long 한국식물생명공학회 2022 Plant biotechnology reports Vol.16 No.1
LRK10L-2 is known to be related to the plant disease response, little information is available about the relationship of LRK10L-2 and fruit ripening. The protein physicochemical properties, conserved domains, gene structures, subcellular locali- zation, expression patterns during grape fruit development and promoter activity of the members of grape LRK10L-2 gene family were explored in this study. A total of 109 LRK10L-2 family gene members were identified, and mainly distributed on chromosome 16. Almost all of them were located in the plasma membrane. Most of the LRK10L-2 genes contain four or five motifs, ranging from 0 to 5 introns and have the cis-acting elements related to hormones in their promoter regions. There were 20 pairs of tandem duplicates and 293 pairs of segmental duplication in LRK10L-2 family genes. It was proved that the expression of LRK10L-2 gene varied at the different fruit development stages of 'Kyoho' and its early-ripening bud mutant, ‘Fengzao’. The subcellular localization of VIT_16s0098g00160 and VIT_16s0098g00400 were in the plasma membrane, and had a significant enrichment of the GUS signal in N.benthamiana leaves for the promoter. The results lay a solid basis for the further functional researches of the LRK10L-2 genes for grape fruit ripening.
Jian Hu,Long Liu,Da-wei Ma 한국물리학회 2014 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.65 No.12
The permanent-magnet synchronous motor (PMSM) system, which is a nonlinear dynamic system,will demonstrate a variety of chaotic phenomena when its parameters or external inputs fallinto a certain area, which will lead to a deterioration of its performance. Thus, chaos should besuppressed or eliminated. In this paper, the property of equilibrium points is analyzed, and thecondition for the occurrence of a Hopf bifurcation in a PMSM system is given based on a mathematicalmodel of the PMSM system with a bifurcation diagram, a Lyapunov exponent map and phaseplane diagrams given. After the drawbacks of the existing control methods have been analyzed, arobust nonlinear feedback controller is designed to control the chaos in the PMSM system with aload torque disturbance. The object is to eliminate the chaos and to drive the system speed to adesired value, Numerical simulation proves the validity of this control method.
( Ping Zhang Wang ),( Ying Xiong ),( Chuan Ma ),( Tai Ping Shi ),( Da Long Ma ) 생화학분자생물학회 (구 한국생화학분자생물학회) 2010 BMB Reports Vol.43 No.11
The c-Jun NH2-terminal kinase (JNK) signaling pathway participates in many physiological functions. In the current study we reported the cloning and characterization of five novel JNK2 transcript variants, which were designated as JNK2 α3, JNK2 α4, JNK2 β3, JNK2 γ1 and JNK2 γ2, respectively. Among them, JNK2 α4 and JNK2 γ2 are potential non-coding RNA because they contain pre-mature stop codons. Both JNK2α3 and JNK2β3 contain an intact kinase domain, and both encode a protein product of 46 kDa, the same as those of JNK2α1 and JNK2β1. JNK2γ1 contains a disrupted kinase domain and it showed a disable function. When over-expressed in mammalian cells, JNK2α3 showed higher activity on AP-1 than that of JNK2β3 and JNK2γ1. Furthermore, JNK2α3 and JNK2β3 showed different levels of substrate phosphorylation, although they both could promote the proliferation of 293T cells. Our results further demonstrate that JNK2 isoforms preferentially target different substrates and may regulate the expression of various target genes. [BMB reports 2010; 43(11): 738-743]
Prevalence and Genotype Distribution of Human Papillomavirus among Women from Henan, China
Wang, Xiao-Chuan,Sun, Liang-Qi,Ma, Li,Li, Hua-Xin,Wang, Xiu-Li,Wang, Xin,Yun, Tian,Meng, Nian-Long,Lv, Da-Le Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.17
Human papillomavirus (HPV) infection has been implicated as a causative of cervical cancer. In the present study, a total of 578 samples from females attending the gynecological outpatient clinic in Henan province, China, were collected and the HPV genotypes were detected by gene chip and flow-through hybridization. Overall, 44.5% (257/578) females were found to be HPV DNA positive, and the high risk HPV (HR-HPV) rate was 35.1% (203/578). The first peak of HR-HPV infection appeared in the >60 year-old group (55.0%), and the second was within the 51-55 year-old group (50.0%) (${\chi}^2$=19.497, p<0.05). HPV 16 was the most prevalent genotype (9.2%), followed by HPV 52 (7.8%), HPV 6 (6.9%), HPV 11 (5.9%) and HPV 42 (5.0%). The single type HPV infection was 30.4%, with the five majority prevalent genotype HPV 16 (16.5%), HPV 52 (14.3%), HPV 6 (12.6%), HPV 42 (8.6%), HPV 31 (5.1%). The multiple-type HPV infections were 14.0%, and HPV 16 was the most prevalent type (29.6%), followed by HPV 52 (24.7%), HPV 6 (22.2%), HPV 11 (22.2%), HPV 42 (17.3%) and HPV 39 (17.3%).
Human HS1BP3 induces cell apoptosis and activates AP-1
( Tai Ping Shi ),( Jie Shi Xie ),( Ying Xiong ),( Wei Wei Deng ),( Jin Hai Guo ),( Feng Wang ),( Da Long Ma ) 생화학분자생물학회(구 한국생화학분자생물학회) 2011 BMB Reports Vol.44 No.6
In the present study, we characterized the function of HS1-binding protein 3 (HS1BP3), which is mutated in essential tremor and may be involved in lymphocyte activation. We found that HS1BP3 localized to the mitochondria and endoplasmic reticulum partially. Overexpression of HS1BP3 induced apoptosis in HEK293T and HeLa cell lines. When these cell lines were transfected with HS1BP3, they exhibited nuclear DNA condensation, externalization of phosphatidylserine (PS), and cleavage of poly ADP ribose polymerase (PARP). Furthermore, suppression of HS1BP3 or HS1 expression attenuates HS1BP3 induced apoptosis. In addition, HS1BP3 enhanced activator protein 1 (AP-1)-mediated transcription in a dose-dependent manner. Therefore, we conclude that HS1BP3 regulates apoptosis via HS1 and stimulates AP-1-mediated transcription. [BMB reports 2011; 44(6): 381-386]