Enhanced Systemic siRNA Delivery Using Reducible Bile Acid-modified PEI for Cancer Therapy

Chae eun PARK,Su yeon LIM,Da hwun KIM,Siyan LYU,Byung deok KIM,Oui bo WHANG,Ji hoon JEONG 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10

Small interfering RNA (siRNA) contributes to RNA interference(RNAi) by inhibiting target gene expression in a sequence-specific manner. siRNA delivery system have become increasingly popular for cancer therapy. In this study, we used a low molecular weight polyethylenimine (PEI, 1.8 kDa) conjugated with deoxycholic acid (DA). A DA-PEI conjugate was further modified with 4-fluorothiophenol (4-FTP) (4-FTP-DA-PEI) to enhance systemic siRNA delivery. The thiophenol group would be involved with disulfide bonds between the polymer chains and siRNA modified with free thiols (thiol-siRNA) to form and π-π interactions between the pendent aromatic groups and coprostane ring of the bile acid. The stabilized TP-DA-PEI conjugate with thiol-siRNA achieved developed intracellular uptake, serum stability, and transfection efficiency. Furthermore, it shows high accumulation of TP-DA-PEI/thiol-siRNA polyplexes and significant tumor growth inhibition effect in tumor-bearing mice after systemic administration.

노인과 대학생의 낙상 위험 분석

정다현,김예희,김지은,이예영,강현주 순천향대학교 기초과학연구소 2021 순천향자연과학연구 논문집 Vol.27 No.1.2

The purpose of this study was to analyze risk of falls between elderly people and college students. The subjects of this study were 25 people aged 70 years or older and 17 college students located in Asan. We measured sensory, integrated balance, nervous, musculoskeletal system and HQR by FRA system (Inbody, Korea). The results of measurement showed significant differences in sensory system (p<.01), integrated balance (p<.001), nervous system (p<.001), and musculoskeletal system (p<.001) between groups. These results suggest that aging makes difference of function of these systems. In our view, counterplans need to be implemented, including exercise intervention to prevent decreases of fall risk,

브랜딩·스타일링·마케팅을 결합한 스페이스 디자인 프로세스 연구 - 작품‘Fill the Red, Feel the Red'를 중심으로 -

은다솜,장효실,최해원,홍지현,이진민,장미정,이성애 숙명여자대학교 디자인연구소 2018 숙명디자인학 연구 Vol.25 No.-

본 연구는 디자인 분야와 마케팅 분야의 전략을 종합하여 전략적 연결점을 찾아내 요소들 간 융합을 이루는 사례를 제시하여 브랜드·스페이스·마케팅에서 새로운 방향성과 아이디어를 제시하는데 목적을 둔다. 이에 본 연구는 2017년 9월~12월까지 ㈜애경과 산업 연계하여 진행된 숙명여대 환경디자인학과 ‘브 랜딩·스타일링·마케팅’ 교과목에서 도출된 ‘fill the red, feel the red’ 프로젝트를 마케팅 및 디자인 프로세스, 결과물을 도출하여 그 과정들을 밝힌다. 아울러 본 연구는 브랜드 스페이스 마케팅에 대한 이론적 고찰을 통해, 브랜드 스페이스(팝업스토어)를 개발 및 스타일링하며 ㈜애경의 화장품 브랜드 ‘LUNA’를 브 랜딩, 스타일, 마케팅적 측면에서 종합적으로 연계하여 도출되는 과정들을 제안함으로서 향후 브랜드 스페이스 디자인과 마케팅 분야에 체계적이고 창의적인 방법들로 결과물을 도출하는데 기초자료로 활용하는데 의의가 있다.

모유 수유아와 조제 분유 영양아에서 수유 방법에 따른 아미노산 농도 비교 및 혈청 트립토판 농도와 수면 유도와의 관계

정다운,김은영,양은석,박상기,박영봉,문경래 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.2

Objective: Sleep behavior is modulated by serotonergic neurons within the brain, and the synthesis and release of serotonin by such neurons is thought to be influenced by the availability of tryptophan, the amino acid precursor of serotonin. Formula-feeding infants have depressed plasma tryptophan concentration relative to breast milk-feeding infants. Because tryptophan alters sleep waking patterns in adults, a study was designed to determine the difference of sleep latency relative to differences in plasma tryptophan and tryptophan : large neutral amino acid (LNAA) ratio between formula-feeding infants and breast milk-feeding infants. Method: 45 newborns who were born in Chosun University Hospital from December 2002 to December 2003 were selected. The newborns were divided to three group, 15 newborns were fed breast milk and 15 newborns were fed formula A, and last 15 newborns were fed formula B. At 6 weeks and 12 weeks, infants were sampled for measuring of serum amino acid level and tryptophan, tryptophan : LNAA ratio. And we taught infants' parents to measure sleep latency that means the time after a feeding to the first episode of active REM sleep that persist ≥ 1 min. Result: 1) At 6weeks, Serum α-amino-n-butyric acid, citrulline, tryptophan level was higher in formula A group than breastmilk group (P<0.05) and proline level was lower than breast milk group (P<0.05). In formula B group, serum n-amino-n-buryric acid, citrulline, cystine, hydroxylysine, hydroxyproline, isoleucine, leucine, lysine, methionine, phenylalanine, taurine, threonine, valine levels were higher than breastmilk group (P<0.05). And Serum isoleucine, methionine, proline, valine level were higher in formula B group than formula A group (P<0.05). 2) At 12 weeks, serum glutamic acid, methionine, ornithine levels were higher in formula group A than breast milk group (P<0.05). Serum β-alanine, aspartic acid, α-amino-n-butyric acid, ethanolamine, glutamic acid, threonine level were higher in formula B group than breast milk group (P<0.05). Serum β-alanine, ethanolamine levels were higher and arginine level was lower in formula B group than formula A (P<0.05). 3) At 6 weeks, serum tryptophan concentration and tryptophan: LNAA ratio were higher in formula A group (P<0.05). Sleep latency was 21 minutes in formula group A, 24 minutes in breast milk group, 25 minutes in formula B group but there was no statistically significance (P>0.05). 4) At 12 weeks, serum tryptophan concentration and tryptophan: LNAA ratio, time after feeding to the first episode of active REM sleep were no difference with each other group. Conclusion: There is significant difference of serum amino acids between breast-feeding infants and formula-feeding infants. And serum tryptophan and tryptophan: LNAA ratio differ between brest-feeding infants and formula-feeding infants. But there is no association between serum tryptophan, tryptophan: LNM ratio and sleep latency.

근피로 후 세 가지 회복 방법

이예영,김지은,서예은,정다연,강현주 순천향대학교 기초과학연구소 2022 순천향자연과학연구 논문집 Vol.28 No.1,2

Muscle fatigue is a symptom that decreases your muscles’ ability to perform over time. It can be associated with a state of exhaustion, often following excessive sports activity or exercise. While exercise is a common cause of muscle fatigue, this symptom can be the result of other health conditions. Muscle fatigue caused by excessive activity limits activities of daily living and difficult to maintain a stable posture. Therefore, the purpose of this study was to investigate the effect of stretching, icing, and sports massage on muscle fatigue recovery.

八味元이 Streptozotocin 糖尿病에 미치는 影響

김희경,문정숙,박귀영,이다미,이은영,장윤경,황미경 曉星女子大學校 藥學大學 學生會 1988 曉星藥誌 Vol.4 No.-

Streptozotocin was injected to rats and rabbits to induce the experimental diabetes mellitus and Palmiwon was administrated to observe the changes of blood sugar, body weight, and serum Zn, Ca, Mg level in order to find out the effect of diabetic tratment by experimental method. And thus, the result was as follows: 1. The blood sugar level of diabetic rat was decreased after administration of Palmiwon. 2. Body weight of diabetic rat was moderately decreased after administratin of Palmiwon. 3. The less streptozotocin, 30 mg/kg, don't easily induce diabetes mellitus in rabbits. We did not find the changes of blood sugar, serum Zn, Ca, Mg level in the rabbits injected by streptozotocin, 30mg/kg of body weight.

자연의 아름다움과 고급스러움이 공존하는 마임 비에타 쇼룸 제안 -오감 (시각, 청각, 후각, 촉각, 미각)을 중심으로-

김보현,이서연,은다솜,엄연정,이진민,장미정,이성애 숙명여자대학교 산업디자인연구소 2017 숙명디자인학 연구 Vol.24 No.-

본 연구는 국내 브랜드 ‘마임'의 뷰티와 연계한 뷰티 쇼룸을 제안함에 있어서 공간속에 오감을 담아내는 일련의 프로세스를 밝히는데 목적이 있다. 이는 브랜드가 소유하고 있는 다양한 감각과 가치가 결여된 채 시각적 요소만이 담긴 브랜드 뷰티 쇼룸이 대다수를 차지하는 오늘날, 이에 대한 문제를 해결하기 위해 ’융합 연출 디자인'적 시점에서 폭넓게 바라보고 브랜드의 정신과 오감의 결합을 통해 이를 해결하고자 하였다. 이에 본 연구는 2017년 3월~6월 15주간 숙명여대 환경디자인학과 <융합연출 디자인>수업에서 진행된 수업의 결과물로서 산학 연계된 화장품 브랜드의 쇼룸디자인 개발 프로세스를 제안한다. 또한 본 연구의 범위는 오감을 담고 있는 뷰티 쇼룸(4D)이 되기까지의 과정 전체로 한다. 그 결과, 마임 뷰티쇼룸은 공간 디자인과 오감을 융합하여 영상에서 공간에 이르기까지 오감의 조화와 브랜드의 정신을 녹여내고 확장시켰다. 끝으로 본 연구는 뷰티 쇼룸 공간 디자인을 제안함에 있어서 오감을 활용하여 통합된 시점에서 그래픽·제품·공간을 개발 및 제시한 것에 의의가 있다. 또한 향후 이와 관련된 쇼룸 공간 개발에 있어서 기초 자료로 활용될 것으로 기대된다.

심사정의 한국화(화훼초충도) 특징 분석

박정은 ( Jeong Eun Park ),정다운 ( Da Uon Chung ),정찬기오 ( Chan Gi Oh Chung ) 경상대학교 중등교육연구소 2015 중등교육연구 Vol.27 No.-

본 연구의 목적은 조선시대의 대표적인 화가인 심사정의 한국화(화훼초충도) 특성을 분석하여 제시하는 것이다. 본 연구의 취지 및 필요성 그리고 연구목적에 근거하여 본 연구자가 설정한 구체적인 연구 문제는 심사정의 작품을 대표해 줄 수 있는 화훼초충도에는 어떤 작품들이 있는가, 심사정의 작품에 등장하는 화훼초충들의 의미는 어떤 것들인가 이다. 본 연구에 사용된 주된 자료는 심사정의 작품과 관련이 있는 석·박사 학위논문자료 16편과 단행본자료 22편, 학술지자료 6편과 신문자료 11종, 그리고 인터넷 사이트 14종 이였다. 연구 결과 자료들에 근거하여 제시할 수 있는 교육적인 의미는 심사정의 작품에서 주된소재가 된 꽃들-패랭이꽃, 모란, 백합, 장미, 원추리, 계화, 국화, 맨드라미, 양귀비, 달개비꽃, 부용화, 연꽃, 해당화 등-이 함축하고 있는 의미들인 젊음, 부귀영화, 장수와 풍요, 모성애와 근심 걱정을 털어버리는 의미인 망우, 아름다움과 향기, 장수와 참고 견딤, 입신출세, 생명력, 무한한 생명 등을 희망적이고 긍정적인 아이디어로 활용하는 것이 필요하다. 그리고 나비, 잠자리, 여치, 메뚜기, 벌, 매미, 베짱이, 사마귀, 무당벌레, 방아깨비 등과 같은 곤충 소재들이 함축하고 있는 의미들인 남녀화합과 행운, 다산과 득남, 벼슬, 부지런함과 충성, 청렴한 선비와 불멸, 출세, 과거급제 등을 희망적이고 긍정적인 아이디어로 활용하는 것이 필요하다고 할 수 있다. 뿐만 아니라, 선비의식과 지조의 의미를 함축하고 있는 괴석이나 지혜의 의미를 함축하고 있는 고슴도치 등도 희망적이고 긍정적인 아이디어로 활용하는 것이 필요하다. The purpose of this study is to suggest features of Korean Painting, Flower-Grass-Insect painted by representative artist Sim Sa-jeong in the period of Joseon dynasty after analyzing Flower-Grass-Insect painting. The specific research problems (the researcher focuses on) of this study based on the intent, necessity, and purpose regarding this study are to analyze certain works of flower-grass-insect painting representing the works of Sim Sa-jeong and specific meaning of flower-grass-insect painting. The main materials used in this study are 16 master′s and doctoral thesis, 22 published books, 6 academic journals, 11 newspaper articles, and 14 internet sites. Educational conclusion based on result of this study is that it is necessary to utilize implicative meaning such as youth, wealth and honor, longevity and richness, maternal love and narcotize one``s anxieties, beauty and fragrance, patience, and tenacious hold on life implied on subject matters of works by Sim Sa-jeong like china pink, peony, lily, rose, day lily, cassia flowers, chrysanthemum, cockscomb, poppy, spiderwort, Hibiscus mutabilis, lotus, sweetbrier and so on as hopeful and positive idea. Also, it is essential to make use of implied meaning such as harmony between man and woman and luck, fecundity, begetting a son, government position, diligence and fidelity, integrity and immortality, success, passing the state examination based on the subject matter of works of Sim Sa-jeong, butterfly, dragonfly, gampsocleis sedakovi obscura, grasshopper, bee, locust, wart. ladybug, long-headed grasshopper as affirmative and bright idea. Furthermore, this study suggests that oddly shaped stone implying the meaning of classical scholar and fidelity and hedgehog with the meaning of wisdom be utilized as positive and hopeful idea.

Case of Sporadic T-cell Lymphosarcoma in Cattle

Kyu-Shik Jeong, Joon-Young Yang, Da-Hee Jeong, Chang-Woo Min, Kyung-Ku Kang, Ah-Young Kim, Eun-Mi Lee, Eun-Joo Lee, Myeong-Mi Lee, Sang-Hyeob Kim, Soo-Eun Sung 충북대학교 동물의학연구소 2013 Journal of Biomedical and Translational Research Vol.14 No.3

We would like to report a case of bovine lymphosarcoma. Parous cattle from a livestock farmhouse were examined for mutinodular masses in the abdominal cavity after slaughter. For clinical signs, animals presented mild leukemic signs but did not have viral or bacterial infection. Grossly, whitish to yellowish smooth masses similar to fat tissue were covered with a thin membrane. A multilobulated mass formed around the arteri, and there was a large quantity of reddish fluid on the cut surface. Histopathologically, a monomorphic population of lymphocytes was observed along with small amounts of cytoplasm, round nuclei with coarsely granular chromatin, and numerous mitotic figures in the samples. In the tumor lesion, uniformly round cells had invaded with abundant neovascularization. Especially, the immunohistochemical phenotype of tumor cells was positive for anti-CD3 and negative for anti-CD8 and anti-CD20. Therefore, morphological analysis diagnosed the mass as a multinodular bovine lymphosarcoma of T-cell origin without any sign of infection by a viral agent.

Variation in Fatty Acid Compositions of Perilla Seeds Collected from Russia

Da Jeong Kim(김다정),Yi Jin Jeong(정이진),Awraris Derbie Assefa,On Sook Hur(허온숙),Na Young Ro(노나영),Jae Eun Lee(이재은),Ho Sun Lee(이호선),Myeong Chul Lee(이명철),Ju Hee Rhee(이주희),Sok Young Lee(이석영),Jung Sook Sung(성정숙 한국약용작물학회 2018 한국약용작물학회 학술대회논문집 Vol.2018 No.2