http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Bioactivities of the Herb Extracts Used for Gamhongroju, a Korean Liqueur
Sae-Rom Lee,Hana Jung,Hyunnho Cho,Changho Jhin,Keum Taek Hwang,Seok Tae Jeong,Tae-Young Kim 한국식품영양과학회 2011 Preventive Nutrition and Food Science Vol.16 No.4
In this study, antioxidative and anti-inflammatory activities of the herb (cinnamon, clove, glehnia root, ginger, violet-root cromwell, licorice, citrus peel and longan) extracts used for gamhongroju, one of the popular liqueurs in Korea, were investigated. Twenty grams of individual herbs were extracted in 60% purified ethanol and freeze-dried. A mixture of the individual herb extracts (HEM) was separately prepared. Cytotoxicity of the individual extracts and HEM on murine RAW264.7 macrophage cells were examined along with their recovering activity on H2O2-treated RAW264.7 cells. Antioxidant and anti-inflammatory activities of the extract-treated cells were determined by measuring superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities, and Trolox equivalent antioxidant capacity (TEAC), nitric oxide (NO) and prostaglandin E2 (PGE2) levels. Violet-root cromwell extract showed the least cytotoxicity in terms of treated concentration. Most of the extracts, below levels of cytotoxicity, recovered the H₂O₂-treated cells. Treatment with some of the extracts increased SOD and GPx activities and TEAC levels while a majority inhibited the production of NO and PGE2 in lipopolysaccharide (LPS)-treated cells.
Eui Jeong Nam,Gyhye Yoo,Joo Young Lee,Myungsuk Kim,Changho Jhin,Yang-Ju Son,Sun Young Kim,Sang Hoon Jung,노주원 생화학분자생물학회 2020 BMB Reports Vol.53 No.7
Exposure to Ultraviolet (UV) light induces photoaging of skin, leading to wrinkles and sunburn. The perennial herb Humulus japonicus, widely distributed in Asia, is known to have antiinflammatory, antimicrobial, and antioxidant effects. However, the physiological activities of isolated compounds from H. japonicus have rarely been investigated. This study focused on the isolation of active compounds from H. japonicus and the evaluation of their effects on photoaging in UVB-irradiated human fibroblast (Hs68) cells. When the extract and four fractions of H. japonicus were treated respectively in UVB-irradiated Hs68 cells to investigate anti-photoaging effects, the ethyl acetate (EtOAc) fraction showed the strongest inhibitory effect on MMP- 1 secretion. From EtOAc fraction, we isolated luteolin-8-C-glucoside (1), apigenin-8-C-glucoside (2), and luteolin-7-O-glucoside (3). These compounds suppressed UVB-induced MMP-1 production by inhibiting the phosphorylation of the mitogen-activated protein kinases (MAPKs) and activator protein-1 (AP-1). When the antioxidant activity of the compounds were estimated by conducting western blot, calculating the bond dissociation energies of the O-H bond (BDE) at different grade, and measuring radical scavenging activity, we found luteolin-8-C-glucoside (1) showed the strongest activity on the suppression of UVB-induced photoaging. These results demonstrate the inhibitory effect of three flavone glycosides derived from H. japonicus on MMP-1 production, MAPK and AP-1 signaling, and oxidative stress; this could prove useful in suppressing UVB induced photoaging.
β-Carotene과 Lycopene의 양자역학 및 화학적 항산화능과 세포 내 산화적 스트레스 보호 효과의 비교
박선영(Sun Young Park),정하나(Hana Jung),진창호(Changho Jhin),황금택(Keum Taek Hwang),곽호경(Ho-Kyung Kwak) 한국식품영양과학회 2017 한국식품영양과학회지 Vol.46 No.9
본 연구는 β-carotene과 lycopene의 양자역학 및 화학적 항산화능과 세포 내 항산화 효과를 분석하였다. β-Carotene과 lycopene의 항산화능을 양자역학적으로 비교한 결과 lycopene의 항산화능이 β-carotene보다 높은 것으로 나타났으며, 이러한 항산화능의 차이는 FRAP로 측정하였을 때도 유사하게 관찰되었다. Trolox와 비교했을 때 β-carotene과 lycopene의 FRAP 수치는 유의적으로 낮았으나 LPS에 의한 세포 내 ROS 생성을 낮추는 데는 두 카로티노이드가 더 우수한 것으로 나타났다. 그러므로 본 연구에서 비교한 β-carotene과 lycopene의 양자역학적 항산화능은 화학적 항산화능을 일부 반영하지만, 실제 세포 내 ROS에 의한 산화스트레스 저하 효과와는 차이가 있는 것으로 나타났다. The aim of this study was to determine the chemical and intracellular antioxidant activities of β-carotene and lycopene and to compare their quantitative structure-activity relationship (QSAR). In our previous study, the second ionization energy of lycopene was higher than that of β-carotene, as calculated by QSAR. Chemical antioxidant activities of β-carotene, lycopene, and Trolox were examined by measuring ferric reducing antioxidant power (FRAP) and 2,2’-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity. Intracellular antioxidant activities were evaluated by intracellular reactive oxygen species (ROS) and DNA fragmentation. The FRAP of lycopene was higher than that of β-carotene (P<0.05), and the two carotenoids had similar antioxidant activities in DPPH radical scavenging activity assay. Trolox had the greatest chemical antioxidant activities (P<0.05). When RAW264.7 cells were treated with lipopolysaccharide (LPS) (100 ng/mL) for 20 h, intracellular ROS and DNA fragmentation significantly increased (P<0.05). RAW 264.7 cells pretreated with β-carotene (4 μM) and lycopene (0.4∼2 μM) for 4 h formed significantly less intracellular ROS than LPS-treated control cells (P<0.05), whereas cells with Trolox did not reduce production of intracellular ROS. In addition, cells pretreated with 2 μM lycopene produced less intracellular ROS than those treated with β-carotene (P<0.05). DNA fragmentation of cells with β-carotene and lycopene was similar to that of LPS-treated control cells as measured by Hoechst staining. The antioxidant ability of lycopene was greater than that of β-carotene in the QSAR, FRAP, and intracellular ROS assays (P<0.05). β-Carotene and lycopene had lower antioxidant activities as measured by FRAP (P<0.05) but higher intracellular protective effects against LPS-induced oxidative stress in comparison with Trolox.
( Dae Hyun Jung ),( Hak-jin Kim ),( Jung-seok Yang ),( Ju Young Lee ),( Hyoung Seok Kim ),( Soo Hyun Park ),( Changho Jhin ),( Teak Sung Lee ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.2
The most widely used soilless techniques are container cultivation which uses a substrate that contains the nutrient solution and continuously feed to the root of the crop. Since the roots are influenced by the substrate parameters such as the water content, the electrical conductivity, and the temperature, The nutrient replenishment and irrigation strategies need to monitor the substrate status. The main objective of this work has been the development of estimation models of EC, water content, and temperature in rockwool substrate using artificial neural network based on Levenberg-Marquardt back-propagation algorithm. We set the 17 inputs for learning by collecting the irrigation and nutrient replenishment status from the hydroponic system, data from drainage solution sensors, the environmental data in the greenhouse, the output data were collected through sensors monitoring substrate. These data for training were measured every 30 s from February 04 to April 03, 2018, training accuracy (R<sup>2</sup>) of three output values were greater than 0.96. The trained neural network models predicted the substrate EC, water content and temperature of test samples, which is randomly selected for 5 days. Test accuracy (R<sup>2</sup>) was 0.92, 0.89 and 0.85 and RMSE was 1.57, 0.07 and 0.45 for water content, EC, temperature, respectively. This method can be used online in tomato cultivation to not only decrease costs related to the installation of sensors but to also make a precise nutrient solution management.
Hana Jung,Ji-Hyun Jeong,Hee Soon Cheon,Jun-Bong Choi,Hyunnho Cho,Changho Jhin,Keum Taek Hwang 한국식품영양과학회 2011 Preventive Nutrition and Food Science Vol.16 No.3
Roasted and retorted (RR) chestnuts develop green pigment spots on their surface during storage. The purpose of this study was to evaluate the cytotoxicity of the green pigment using RAW 264.7, MOLT-4, KATOⅢ and HT-29 cells. The pigment scraped from RR chestnuts (GP), whole RR chestnuts with green pigment spots (GC), whole RR chestnuts without green pigment (WC) and roasted and frozen stored chestnuts (FC) were extracted in 10% DMSO. MOLT-4 cells were less resistant to the cytotoxicity of the chestnut extracts than the RAW 264.7 cells. The GP extracts did not show different responses against H2O2-induced oxidative stress and LPS-induced NO production compared to the other extracts. The chestnut extracts did not have proliferative activity on either of the KATOⅢ or HT-29 cells (p>0.05). Our results from the comparison of the green pigment produced on the surface of the RR chestnuts to chestnuts that do not develop the green pigment suggest that the pigment may not be harmful in terms of either cytotoxicity towards immune cells or proliferation of gastrointestinal cancer cells.