Carotenoids 식이와 알코올이 혈액학적 성분과 간조직에 미치는 영향에 관한 연구

조만희,김연선,이상한,우기민,장예진,김창세 순천향의학연구소 1999 Journal of Soonchunhyang Medical Science Vol.5 No.2

Alcohol is well known agent which can damage the human tissues such as liver via stimulating lipid peroxidation and storage, denaturation of macromolecules, and inhibiting protein metabolism. On the other hand, carotenoids in addition to vitamins A, C, E and lipoic acid, play important roles in protecting these oxidative damages as well as preventing the production of free radicals. This study was carried out to elucidate the precise effects of alcohol administration into rats on the antioxidative functions of dietary carotenoids and isolated β-carotene, and to find out any parameters to uncover more detailed biochemical mechanisms of these agents. For these purposes, two different approaches were performed: 1) examination of the changes in hematological parameters (e.g., total proteins, A/G ratio, ALT/AST et. al) and statistical correlations among inter- and intragroups, 2) examination of the histopathological changes by an electron microscope. The results were analyzed and summarized as following; (1) Carrot diet for one week caused a slight increase in albumin. A/G ratio and AST levels, and a slight decrease in ALT, ALP, BUN and uric acid levels. Two-week alcohol administration following carrot diet increased total proteins, albumin A/G ratio and BUN levels. However, both groups were shown to have little significant changes in cholesterol concentrations. (2) Cheese diet for one week caused a significant increase in total proteins, albumin, AST, ALP, uric acid and total cholesterol levels, and a significant decrease in A/G ratio and glucose concentrations. However, two-week β-carotene diet following cheese intake increased total proteins albumin, A/G ratio, BUN and total cholesterol levels, whereas AST, ALT, ALP and uric acid levels were decreased. (3) Alcohol administration for one week caused an increase in AST activities and a decrease in total proteins, albumin, ALT, ALP and BUN levels. A successive β-carotene diet following alcohol administration increased total proteins, albumin, ALT, ALP and BUN levels, whereas decreased AST and uric acid levels. However, there were not significant changes in A/G ratio, glucose and total cholesterol levels in both groups. (4) β-carotene diet for one week caused a slight increase in albumin, glucose, AST and uric acid levels, and a slight decrease in ALP and BUN levels. Two-weeks alcohol administration following β-carotene diet increased albumin, glucose, BUN and total cholesterol levels, whereas decreased AST, ALT, ALP and uric acid levels. (5) Although the pathological investigation on the liver did not reveal significant changes, cheese diet group (CH-BC/1-CH) was shown to have some lipid deposits. Some results were unexpected and different from typical hematological changes shown by other researchers. Nonetheless, these results strongly suggest that the liver damage or hepatism caused by alcohol intake affects many kinds of biochemical metabolisms, which results in significant changes in many hematological parameters. In addition, dietary carotenoid and isolated β-carotene were shown to have protective roles against the biochemical changes by alcohol intake.

Which Environmental Factors Caused Lammas Shoot Growth of Korean Red Pine?

Lee, Chang-Seok,Song, Hye-Gyung,Kim, Hye-Soo,Lee, Bit-Na-Ra,Pi, Jeong-Hoon,Cho, Yong-Chan,Seol, Eun-Sil,Oh, Woo-Seok,Park, Sung-Ae,Lee, Seon-Mi The Ecological Society of Korea 2007 Journal of Ecology and Environment Vol.30 No.1

Lammas growth, a rare phenomenon for Korean red pine (Pinus densiflora), occurred in 2006. Lammas shoots showed higher frequency and longer length in Seoul's hotter urban center than in urban boundary or suburban forest sites. Frequency and length showed a close correlation with urbanization density and vegetation cover expressed in NDVI. Air temperature in the late summer of 2006 was more than $1^{\circ}C$ higher than an average year. Of the predominant environmental signals that modulate bud flush, only temperature changed significantly during the year. Differences in temperature between the urban centers, urban boundaries and suburban forests correlated with varying land-use density. The rise in temperature likely spurred lammas growth of the Korean red pine. Symptoms of climate change are being detected throughout the world, and its consequences will be clearer in the future. Considerate interest in the responses of ecological systems to the variable changes is required to prepare for unforeseeable crises. Monitoring of diverse ecological phenomena at Long Term Ecological Research sites could offer harbingers of change.

기후변화와 키메라현상에 대한 계층 간의 관계인식

백정애(Jungae Baik),김인경(In Kyoung Kim),김진영(Jin Yung Kim),노용환(Yong Hwan Noh),배선영(Sunyoung Bae),우명원(Myoung Won Woo),장혁기(Hyukki Chang),조성돈(Sung Don Cho),장매희(Maehee Chiang) 인간식물환경학회 2011 인간식물환경학회지 Vol.14 No.3

This study was investigated to research the basis of interdisciplinary fusion through the survey on the basis of the answers of professionals and general public, gender, humanities, science and engineering about the question of climate change and chimera which is one of the various phenomena that affect the human life. All respondents’ perceptions of climate change was high and natural disasters was showed the most destruction according to climate change. The answer of awareness of climate change and the damage, chimerism was significant differences between age groups sex, specialized domain-specific individuals. In addition, a group of respondents for science and engineering professionals was higher for awareness of climate change and the chimerism and heir negative opinion for chimerism was relatively low in comparison of other research groups.

PET/CT 검사 환자의 피폭선량 경감을 위한 연구

김봉수,표성재,조용귀,신채호,조진우,김창호,Kim, Bong-Su,Pyo, Sung-Jai,Cho, Yong-Gyi,Shin, Chai-Ho,Cho, Jin-Woo,Kim, Chang-Ho 대한핵의학기술학회 2009 핵의학 기술 Vol.13 No.3

목적 : 환자의 피폭선량 감소를 위해 PET/CT검사 시Pitch를 조절하여 환자의 피폭선량을 줄일 수 있는 방법에 대해 고려해 보고 Pitch 조정이 CT 영상과 PET의 SUV값에 영향을 주어 변화가 있는지를 살펴 보고자 한다. 방법 : Siemens사의 Biograph Positron Emission Tomography (PET) Scanner (CT 형식 : TRCT-240-130 (WCT-240-130)을 사용하였다. 환자의 피폭선량 평가로는 CT 조사선량 측정기인 PTW-DIADOS 11003/1383를 사용하여 선량을 측정하였고 Pitch 조정이 CT 영상에 미치는 영향을 알아보기 위해 AAPM Standard Phantom을 이용하여 pitch 변화에 따른 CT 영상의 공간 분해능을 측정하여 비교하였다. 그리고, PET source consists of a solid radioactive cylinder phantom을 사용하여 Pitch 변화에 따른 Fusion 영상의 SUV값을 산출하여 PET/CT 영상에서 SUV값이 변하였는지 확인하였다. 결과 : 2slice CT scanner에서는 Pitch가 0.7~1.3까지는 방사선량이 크게 떨어지나 1.5~1.9까지는 방사선량의 감소가 작아졌으며 Pitch값이 커질수록 환자의 피폭선량이 작아지는 것을 알 수 있었다. 그리고 Pitch값의 증가에 따른 SUV값의 변화는 거의 없었으며. Pitch값이 PET SUV값에 영향을 주지 않는다는 것을 알 수 있었다. Pitch의 변화가 CT 영상에도 크게 영향을 주지 않는 것을 알 수 있었다. 결론 : 위의 결과로 PET/CT를 사용하는 병원은 영상의 왜곡이 없고 PET SUV값에 영향을 주지 않는 범위 내에서 각 병원에 맞는 Pitch값을 찾아서 환자의 피폭 경감을 위해 노력해야 할 것이다. 그리고 Multi-detector를 가진 CT scanner인 경우에 모두 해당 될 것이라 생각되며 향후 다른 장비에서도 이와 같은 실험이 필요하다 하겠다. Purpose: As the number of patients has increased since the installation of a PET/CT, we are now examining about 2500-3000 annually. We have realized that if we properly adjust a pitch under the same condition of a CT during a PET/CT exam, radiation quantity that reaches the patient can change. In order to reduce the exposure dose of a patient, the research examines a method of reducing the exposure dose of a patient by controlling the pitch during a PET/CT exam, viewing whether the adjustment of the pitch influences CT image and PET SUV. Methods: The equipment used is a Biograph Positron Emission Tomography (PET) Scanner (CT type: TRCT-240-130 (WCT-240-130)) of Siemens company. For the evaluation of exposure dose of a patient, we measured radiation quantities using a PTW-DIADOS 11003/1383, which is a CT radiation measurement instrument used by Siemens. We measured and analyzed the space resolutions of CT images caused by the change of pitches using an AAPM Standard Phantom in order to see how the adjustment of pitches influenced the CT images. In addition, in order to obtain SUVs caused by each change of pitches using a PET source made with a solid radioactive cylinder phantom, we confirmed whether the SUVs changed in the PET/CT images by calculating the SUVs of the fusion images caused by the change of pitches after obtaining CT and PET images and finishing the test. Results: 2slice CT scanner showed that radiation quantities largely dropped when pitches ranged from 0.7 to 1.3 and that the reduction of radiation quantities were smaller when pitches ranged from 1.5 to 1.9. That is, we found that the bigger pitch values are the smaller the radiation quantities of a patient are. Moreover, we realized that there is no change of SUVs caused by the increase of pitches and that pitch values do not influence PET SUVs and the quality of CT images. It is judged that using 1.5 as a pitch value contributes to the reduction of exposure dose of a patient as long as there is no problem in the quality of an image. Conclusions: When seeing the result of the research, hospital using a PET/CT should make an effort to reduce the exposure dose of a patient seeking pitch values appropriate for their hospital within the range in which there is no image distortion and PET SUVs are not influenced from pitches. We think that the research can apply to all multi-detectors having a CT scanner and that such a research will be needed for other equipments in the future.

Thermoplasma acidophilum의 20S Proteasome에 의한 ATPase-활성적 단백질분해에 관한 연구

우기민,장예진,조만희,김창세,김완종,조성호,이상한 순천향의학연구소 1999 Journal of Soonchunhyang Medical Science Vol.5 No.1

The eukaryotic 26S proteasome is an ATP/ubiquitin-dependent proteolytic complex consisting of the 20S core particle and 19S ATPase complex. However, because of its complexity and unstable properties, this study was carried out to present more simple and stable model for the ATP-activated proteolytic complex in prokaryotes which can take the place of the eukaryotic 26S proteasome. For this purpose, recombinant Thermoplasma 20S proteasome (T20S) and Methanococcus MS4, a sequence homolog of one ATPase subunit in the 19S ATPase complex, were successfully isolated from Escherichia coli (E. coli). The α and β subunits of T20S expressed in E. coli could assemble for themselves, and showed the peptide-hydrolyzing activity. Whereas both T20S and R20S (the 20S complex from rabbit skeletal muscle) had the highest peptidase activity against Suc-LLVY-AMC, a good substrate for chymotrypsin-like peptidase activity, the specific activity of T20S was slightly lower than that of R20S. In addition, several reagents such as KCI, SDS, and ovalbumin were shown to have different effects on the peptidase activities between T20S and R20S. When the ATPase activity of the purified MS4 were assayed , the Km for ATP was about 0.5 mM, and casein could stimulate the activity more than 2 fold without the change in Km. This result implicates that protein-activated ATPase may induce the conformational change of casein, and therefore suggests that MS4 ATPase may activate the proteolytic activity of the 20S proteasome via accelerating the recognition and translocation of the protein substrates.

Characterization of changes in global gene expression in the brain of neuron-specific enolase/human Tau23 transgenic mice in response to overexpression of Tau protein.

Woo, Jong-Min,Park, So Jung,Kang, Ho Il,Kim, Byoung Guk,Shim, Sun Bo,Jee, Seung Wan,Lee, Su Hae,Sin, Ji Soon,Bae, Chang Joon,Jang, Mee Kyung,Cho, Chunghee,Hwang, Dae Youn,Kim, Chuel Kyu D.A. Spandidos 2010 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.25 No.5

<P>Tau is a neuronal phosphoprotein responsible for the formation of the neurofibrillary tangles in Alzheimer's disease. To characterize the changes in global gene expression in the brain of transgenic mice that overexpress human Tau23 protein in response to the increase of Tau23 phosphorylation, total RNA extracted from the hippocampus of 12-month-old transgenic and wild-type mice was converted to cDNA, labeled with biotin and hybridized to oligonucleotide microarrays. The microarray results were confirmed by real-time RT-PCR and Western blotting method. It was determined that 43 genes were up-regulated and 8 genes were down-regulated by Tau23 in transgenic mice compared to controls, based on the arbitrary difference in the 2-fold change. Among the up-regulated transcripts, those encoding for transporter and oxidoreductase were dramatically over-represented, followed by those related to regulatory molecule, cytoskeletal protein, signaling molecule, and extracellular matrix protein. Genes encoding for transcription factor, regulatory molecule, miscellaneous function, and chaperone were significantly reduced in the down-regulated group. The major genes in the up-regulated categories included Ecrg4, Folr1, Defb11, Aqp1 and Soctdc1. The major genes in the down-regulated categories were Ncor1, Gpm6a, and Hspd1. These results indicate that the microarray analysis identifies several gene functional groups and individual genes that respond to a sustained increase in Tau23 phosphorylation levels in the brain of transgenic mice. In addition, the results suggest the microarray test is a useful tool for increased understanding of the role of Tau23 protein in regulating neurodegenerative disorders.</P>

Studies on N-Ethyl-N-nitrosourea Mutagenesis in BALB/c Mice

Cho, Kyu-Hyuk,Cho, Jae-Woo,Song, Chang-Woo Korean Society of ToxicologyKorea Environmental Mu 2008 Toxicological Research Vol. No.

N-ethyl-N-nitrosoures (ENU) is effective in inducing hypermorphic mutation as well as hypomorphic and antimorphic mutations. Therefore, this mutagen is used to the production of mutant in the mice. In order to perform an effective ENU mutagenesis using BALB/cAnN mice, determination of optimal dosage and dosage regimen of ENU is necessary. And this study tried to develop a suitable screening method and searched for novel and various mutants as model animals in phenotypedriven ENU mutagenesis. We have carried out dosage regimen for mutagenizing dose of 200 mg/kg ENU in the BALB/c mice. Total screened mice were 30,133. As the results of Esaki and Cho's Phenotype Screening, we got 2,516 phenotypic and behavior abnormalities in $G_1,\;G_2\;and\;G_3$ mice. One hundred thirty five $G_1$ phenodeviants were tested for inheritance and 16 dominant mutants were discovered. Forty two recessive mutants were also found in tested 201 micropedigrees. Early-onset mutant mice included the dysmorphology of face, eye, tail, limb, skin, and foot and abnormal behavior like circling, swimming, head tossing, stiff-walking, high cholesterol level, and tremor etc. In this study we could effectively screen $G_3$ recessive mutants. The frequent and concise early-onset screening before weaning will be available for ENU mutagenesis.

Minimize makespan of permutation flowshop using pointer network

Cho Young In,Nam So Hyun,Cho Ki Young,Yoon Hee Chang,Woo Jong Hun 한국CDE학회 2022 Journal of computational design and engineering Vol.9 No.1

During the shipbuilding process, a block assembly line suffers a bottleneck when the largest amount of material is processed. Therefore, scheduling optimization is important for the productivity. Currently, sequence of inbound products is controlled by determining the input sequence using a heuristic or metaheuristic approach. However, the metaheuristic algorithm has limitations in that the computation time increases exponentially as the number of input objects increases, and separate optimization calculations are required for every problem. Also, the heuristic such as dispatching algorithm has the limitation of the exploring the problem domain. Therefore, this study tries a reinforcement learning algorithm based on a pointer network to overcome these limitations. Reinforcement learning with pointer network is found to be suitable for permutation flowshop problem, including input-order optimization. A trained neural network is applied without re-learning, even if the number of inputs is changed. The trained model shows the meaningful results compared with the heuristic and metaheuristic algorithms in makespan and computation time. The trained model outperforms the heuristic and metaheuristic algorithms within a limited range of permutation flowshop problem.

신도시 개발에 따른 첨두유출량과 토양유실량 변화에 관한 연구 -목포시 남악 신도시 개발지를 대상으로-

우창호 ( Chang Ho Woo ),조남열 ( Nam Yui Cho ) 한국환경영향평가학회 2002 환경영향평가 Vol.11 No.4

The purpose of this study is to explore the hydrological impacts and soil loss variation due to the land use change of Namak New Town development area. The analysis of hydrological effects and soil loss variation has been carried out using GIS in this study. In order to estimate the peak runoff volumn, the Rational Method which is the most popular technique to predict runoff amounts is used. To estimate the soil loss in the study area, Universal Soil Loss Equation(USLE), which is one of the most comprehensive and useful technique to predict soil ersion is adopted. The result of this study has shown that the peak runoff volume and the total soil loss increse according to the land use change. The peak runoff volume and the total soil loss have been incresed about 2 time and about 48 times more than that of pre development. The increasing of the peak runoff volume can be effective erosion, flooding and so on. A careful city planning is the first essential step to minimize the environmental impacts and to construct the ecological city.

폭발하중을 받는 연료저장 사일로의 동적 파괴거동 분석

조우민(Woo-Min Cho),장윤석(Yoon-Suk Chang) 대한기계학회 2022 大韓機械學會論文集A Vol.46 No.3

사용후핵연료 임시 저장시설인 사일로는 콘크리트, 철근 및 라이너로 구성되어 구조적 강도 및 방사선 차폐기능을 수행한다. 예기치 못한 사고 상황에서 독성 물질 누출 방지를 위해 각 부품의 건전성은 유지되어야 한다. 본 연구는 수치해석 기법을 활용하여 폭발하중이 가해지는 연료저장 사일로의 동적 파괴거동을 분석하고 적절한 파손기준을 제시하는 것이다. 재료의 비선형성과 변형률 속도 효과를 고려하여 연료저장 사일로의 손상, 변형률과 회전각도를 조사하였다. 폭발조건 및 콘크리트 압축강도를 변화시켜 해석을 수행하였고 파손기준과 해석 결과를 비교하였다. 모든 경우에서 철근과 라이너는 파손되지 않았으나 콘크리트는 일부의 경우 파손되었다. 콘크리트의 외부 하중에 대한 일차 방호 기능 및 해석 결과의 보수성을 고려하여 해당 파손기준을 사일로 평가를 위한 기준으로 제시하였다. Fuel storage silo, which is an interim dry storage facility of spent nuclear fuel, provides structural strength and radiation shielding. In this study, a numerical analysis was used to analyze the dynamic failure behavior of a fuel storage silo under blast loads and to present appropriate failure criteria. Damage, strain, and support rotation of the fuel storage silo were investigated considering the non-linearity and strain effect of the materials. The explosive condition and concrete strength were changed. The analyses results were compared with the failure criteria of each component. In all cases, reinforcement and liner were not damaged, but the concrete wall exceeded the failure criteria at several analyses. Considering the conservative characteristics of nuclear facilities and the importance of concrete to protect spent nuclear fuel while serving as a primary barrier from external loadings, concrete failure criteria was presented as criteria for silo evaluation.