아세틸 콜린 유발 검사시 허혈성 심전도 변화와 흉통의 발생이 내피세포 기능장애에 미치는 영향

최철웅(Cheol Ung Choi),나승운(Seung-Woon Rha),김선원(Sun Won Kim),나진오(Jin Oh Na),임홍의(Hong Euy Lim),김진원(Jin Won Kim),김응주(Eung Ju Kim),한성우(Seong Woo Han),박창규(Chang Gyu Park),서홍석(Hong Seog Seo),오동주(Dong Joo Oh) 대한임상노인의학회 2010 대한임상노인의학회지 Vol.11 No.4

연구배경: 아세틸 콜린 유발 검사시 발생하는 흉통과 허혈성 심전도 변화가 아세틸 콜린에 의해 발생하는 관상동맥의 내피세포 기능에 어떤 영향을 주는지 알아보고자 했다. 방법: 흉통으로 내원하여 관상동맥 조영술과 아세틸 콜린 유발검사를 시행받은 1,085명의 환자를 대상으로 하였다. 아세틸 콜린 유발 검사를 하는 동안 허혈성 심전도 변화 및 흉통의 발생 유무에 따른 관상동맥 내피세포 기능의 특징을 비교하였다. 결과: 539명의 환자에서 아세틸 콜린 검사시 내피세포 기능 장애가 발생 하였다. 허혈성 심전도 변화가 있었던 그룹이 심전도 변화가 없었던 그룹보다 흉통이 더 많았고(78.1% vs. 60.8%, P=0.007), 관상동맥 조영술상 다발성 관상동맥 경련이 빈번하였고(59.4% vs. 40.6%, P=0.004), 관상동맥의 경련 정도도 더 심하였다(64.1% vs. 46.5%, P=0.006). 흉통이 있었던 그룹이 흉통이 없었던 그룹보다 허혈성 심전도 변화의 빈도가 더 높았다(14.7% vs. 7.5%, P=0.007). 또한 흉통이 있었던 그룹이 관상동맥 경련이 다발성으로 발생하는 경우가 많았고 (50.7% vs. 29.5%, P<0.001), 미만성으로 발생하는 경우가 많았고(87.1% vs. 75%, P<0.001), 관상동맥의 경련의 정도도 더 심하였다(51.6% vs. 43.5%, P=0.041). 결론: 아세틸 콜린 유발검사시 발생하는 허혈성 심전도 변화와 흉통은 관상동맥 경련의 정도와 경련이 발생하는 관상동맥 개수와 연관이 있다. Background: ECG changes and chest pain during acetylcholine (Ach) provocation tests may constitute important clinical parameters for Ach-induced endothelial dysfunction. We investigated the association between ischemic ECG changes and chest pain during the Ach-provocation test and angiographic characteristics of Ach-induced endothelial dysfunction. Methods: A total of 1,085 patients with anginal symptoms underwent diagnostic coronary angiography (CAG) and Ach-provocation tests. We compared angiographic characteristics of Ach-induced endothelial dysfunction according to the presence of ECG change and chest pain. Results: A total of 539 patients experienced Ach-induced endothelial dysfunction. Patients who experienced ECG change group suffered more frequent chest pain (78.1% vs. 60.8%, P=0.007), angiographically more frequent multiple coronary artery spasm (59.4% vs. 40.6%, P=0.004), and more severe coronary artery spasm (64.1% vs. 46.5%, P=0.006) than patients without ECG change. However, there was no difference in the length of endothelial dysfunction between the groups. The incidence of ischemic ECG changes in patients with chest pain was higher than in patients without chest pain (14.7% vs. 7.5%, P=0.007). Patients who experienced chest pain more often experienced multiple (50.7% vs. 29.5%, P<0.001), diffuse (87.1% vs. 75%, P<0.001), and severe coronary artery spasm (51.6% vs. 43.5%, P=0.041) than patients without chest pain. Conclusion: Ischemic ECG changes and chest pain during the Ach-provocation test are associated with multi-vessel involvement and severe coronary artery spasm. Chest pain is associated with the length of endothelial dysfunction, but ischemic ECG change is not.

Gene Expression Analysis of the Bromobenzene Treated Liver with Non-hepatotoxic Doses in Mice

Lim, Jung-Sun,Jeong, Sun-Young,Hwang, Ji-Yoon,Park, Han-Jin,Cho, Jae-Woo,Song, Chang-Woo,Kim, Yang-Seok,Lee, Wan-Seon,Moon, Jin-Hee,Han, Sang-Seop,Yoon, Seok-Joo The Korean Society of Toxicogenomics and Toxicopro 2005 Molecular & cellular toxicology Vol.1 No.4

Bromobenzene (BB) is well known hepatotoxicant. Also, BB is an industrial solvent that arouses toxicity predominantly in the liver where it causes centrilobular necrosis. BB is subjected to Cytochrome P450 mediated epoxidation followed by either conjugation with glutathione, enzymatic hydrolysis or further oxidation. In this study, we focused on BB-induced gene expression at non-hepatotoxic dose. Mice were exposed to two levels of BB, sampled at 24 h, and hepatic gene expression levels were determined to evaluate dose dependent changes. When examining the toxic dose of BB treated group in other previous studies, genes related to heat shock protein, oxidative stress, and drug metabolism are expressed. Compared to these results, our study, in which non-toxic dose of BB was administrated, showed similar patterns as the toxic conditions above. The purpose of the study was to select genes that showed changes in relation to the differing dose through confirmation of the difference within transcriptomic boundaries, but those that are not detected by the existing classic toxicology tools in non-hepatotoxic dose.

Effect of Photoperiod, Temperature and True-leaf Stage in Bolting Rate of Chicory (Cichorium intybus L. var. sativus)

Lim,Jung-Dae,Seo,Jeong-Sik,Lee,Hyeon-Yong,Kim,Jong-Dai,Lee,Jin-Ha,Yu,Chang-Yeon 한국자원식물학회 2004 Plant Resources Vol.7 No.1

Root chicory (Cichorium intybus L. var. sativus) is potential alternative medicinal and sugar crop which accumulates a high amount of linear polyfructan, inulin in its roots. A problem in root production is that over-wintered stock plants often flower. Once the plant becomes reproductive, stem elongation and root growth slows and floral buds arise from every node, rendering the plants useless for propagation. The objectives of this research was to examine the effectiveness of manipulating environmental factors containing photoperiod, temperature and number of leaf states. The experiment was performed in growth chamber to create two photoperiods (8 h, and 16 h) with three temperature regimes (5℃/3℃, 10℃/8℃, and 15℃/13℃ day/night temperature) for a total of six treatments on three type of true-leaf stage of plant. Data of bolting rate, shoot and root length, shoot and fresh weight was invetigated in each treatments. This is the first report on changes in bolting rate and shoots and roots production during a whole growing season and differences in the effect of cold and photoperiod treatment depending on the true-leaf stage of plant.

The LIM-only transcription factor LMO2 determines tumorigenic and angiogenic traits in glioma stem cells

Kim, S-H,Kim, E-J,Hitomi, M,Oh, S-Y,Jin, X,Jeon, H-M,Beck, S,Jin, X,Kim, J-K,Park, C G,Chang, S-Y,Yin, J,Kim, T,Jeon, Y-j,Song, J,Lim, Y C,Lathia, J D,Nakano, I,Kim, H Macmillan Publishers Limited 2015 CELL DEATH AND DIFFERENTIATION Vol.22 No.9

Glioblastomas (GBMs) maintain their cellular heterogeneity with glioma stem cells (GSCs) producing a variety of tumor cell types. Here we interrogated the oncogenic roles of Lim domain only 2 (LMO2) in GBM and GSCs in mice and human. High expression of LMO2 was found in human patient-derived GSCs compared with the differentiated progeny cells. LMO2 is required for GSC proliferation both in vitro and in vivo, as shRNA-mediated LMO2 silencing attenuated tumor growth derived from human GSCs. Further, LMO2 is sufficient to induce stem cell characteristics (stemness) in mouse premalignant astrocytes, as forced LMO2 expression facilitated in vitro and in vivo growth of astrocytes derived from Ink4a/Arf null mice and acquisition of GSC phenotypes. A subset of mouse and human GSCs converted into vascular endothelial-like tumor cells both in vitro and in vivo, which phenotype was attenuated by LMO2 silencing and promoted by LMO2 overexpression. Mechanistically, the action of LMO2 for induction of glioma stemness is mediated by transcriptional regulation of Jagged1 resulting in activation of the Notch pathway, whereas LMO2 directly occupies the promoter regions of the VE-cadherin gene for a gain of endothelial cellular phenotype. Subsequently, selective ablation of human GSC-derived VE-cadherin-expressing cells attenuated vascular formation in mouse intracranial tumors, thereby significantly prolonging mouse survival. Clinically, LMO2 expression was elevated in GBM tissues and inversely correlated with prognosis of GBM patients. Taken together, our findings describe novel dual roles of LMO2 to induce tumorigenesis and angiogenesis, and provide potential therapeutic targets in GBMs.

소아 IgA 신병증의 장기 추적(평균 10.8년)에 따른 임상 경과 및 병리학적 변화

문창민,김병길,임범진,송지선,정현주,Moon, Chang-Min,Kim, Pyung-Kil,Lim, Beom-Jin,Song, Ji-Sun,Jeong, Hyeon-Joo 대한소아신장학회 2010 Childhood kidney diseases Vol.14 No.2

목 적 : 소아 IgA 신병증의 임상 양상 및 병리학적 분류(Haas 분류)와 임상 경과와의 상관 관계를 조사하여 이들이 예후를 반영할 수 있는지 알아보고자 하였다. 방 법 : 병리학적으로 IgA 신병증으로 진단받고 추적 중이던 환자들 중 추적 신생검을 시행한 20명의 환자를 대상으로 후향적으로 자료를 분석하였다. 결 과 : 최초 신생검시 평균 9.5세였고 남자 16명, 여자 4명이었으며, 최종 조직 검사까지 평균 10.8년이 경과하였다. 안지오텐신 전환효소 억제제 등으로 치료를 지속한 후 정상뇨 소견, 정상 혈압, 정상 크레아티닌 청소율(CCr)을 보이는 임상적 관해 상태의 환자는 10명(50%)이었으며, 비관해 상태의 환자 10명(50%)도 초기와 비교하여 호전된 경과를 보였고, CCr이 중등도 이상 저하되거나 말기 신질환 양상을 보이는 환자는 없었다. 최초 Haas 분류는 임상경과와 상관 관계가 없었다. 초기 고혈압은 5명(25%)에서 보였고 이는 임상 경과(P =0.010) 및 최종 Haas 분류(P =0.007)와 유의성이 있었다. 초기 CCr의 중등도 저하는 Haas 분류와 유의성이 있었으나(P =0.048), 임상 경과와는 상관관계가 없었다. 결 론 : 소아 IgA 신병증의 추적 기간 중 임상 경과는 양호하나 최초 Haas 분류로 임상 경과를 예측할 수는 없었으나 적극적인 조기 진단 및 치료로 말기 신질환으로의 진행을 지연시킬 수 있을 것으로 보인다. 초기 고혈압은 임상 경과 및 최종 Haas 분류와 유의한 상관관계를 보여 적합한 예후인자로 보인다. 최초 병리학적 소견은 임상 경과를 반영하지 못하나, 추적 Haas 분류가 예후를 반영할 수 있을지에 대해서는 보다 많은 추적 관찰 및 추적 신생검을 통한 연구가 있어야 할 것으로 보인다. Purpose : We know little about the natural course of IgA nephropathy (IgAN) in association with histologic changes especially in children. We investigated clinicopathologic features with long-term follow-up biopsy to clarify the outcomes and prognostic indicators for childhood IgAN. Methods : From our patients' medical records, we retrieved 20 patients with IgAN, to whom renal biopsies had been performed for the initial diagnosis and follow-up to find out any histologic changes. Initial and follow-up biopsies were classified by Haas classification. The changes of these parameters were compared with the evolution of clinical features. Results : Patients were treated with angiotensin-converting enzyme inhibitors in combination with angiotensin receptor blockers (in subclass II or above) and short-term cyclosporine A(in patients showing nephrotic syndrome). Histologic improvement in 7 cases and deterioration in 3 cases were observed. At the time of last biopsy, 10 cases (50%) showed clinical remission and the others showed improved clinical features. These clinical outcomes did not correlate with initial Haas classifications. Hypertension at onset observed in 5 cases (25%) revealed significant correlation with clinical outcome (P =0.01) and last Haas classification (P =0.007). None of the cases showed progression to CRF or ESRD. Conclusion : During a mean follow-up of $10.8{\pm}3.4$ years, childhood IgAN showed good clinicopathologic outcome. Hypertension at onset was only a strong predictor of clinicopathologic outcomes, but initial Haas classification cannot predict outcomes in children. Histologic change of IgAN in long term follow-up period cannot be completely predicted by clinical data and vice versa. Therefore, a renal biopsy should be considered as a part of follow-up plan.

A two-photon ratiometric probe for hydrogen polysulfide (H₂S_n): Increase in mitochondrial H₂S_n production in a Parkinson’s disease model

Choi, Hyeon Jin,Lim, Chang Su,Cho, Myoung Ki,Kang, Ji Su,Park, Soo Jin,Park, Sang Myun,Kim, Hwan Myung Elsevier 2019 Sensors and actuators. B, Chemical Vol.283 No.-

<P><B>Abstract</B></P> <P>Hydrogen polysulfide (H<SUB>2</SUB>S<SUB>n</SUB>, n>1), which is primarily generated during the crosstalk between H<SUB>2</SUB>S and reactive species (ROS and RNS), is receiving increasing attention in biochemical research. H<SUB>2</SUB>S<SUB>n</SUB> is mostly generated in the mitochondria, and abnormal mitochondrial function and oxidative stress are directly related to many disorders including Parkinson's disease (PD). We now report a two-photon fluorescent probe (<B>SPS-M1</B>) for in situ detection of H<SUB>2</SUB>S<SUB>n</SUB> and its application to a PD model to account the H<SUB>2</SUB>S<SUB>n</SUB> levels. The probe exhibited selective and fast response to H<SUB>2</SUB>S<SUB>n</SUB> along with a marked blue-to-green color change. <B>SPS-M1</B> is sensitive enough to quantitative detection of endogenous H<SUB>2</SUB>S<SUB>n</SUB> content in mitochondria using two-photon microscopy (TPM). Ratiometric TPM imaging of live neurons and brain slices using <B>SPS-M1</B> revealed that H<SUB>2</SUB>S<SUB>n</SUB> production is increased to a greater extent in the A53 T α-synuclein (α-syn) overexpressing model than in the wild-type control. These findings suggest that the interactions of H<SUB>2</SUB>S and the increased ROS caused by α-syn overexpression may generate more H<SUB>2</SUB>S<SUB>n</SUB>. By employing our recently published TP probe for mitochondrial H<SUB>2</SUB>S, we also found the relationship between the H<SUB>2</SUB>S<SUB>n</SUB> and H<SUB>2</SUB>S; increased H<SUB>2</SUB>S<SUB>n</SUB> and decreased H<SUB>2</SUB>S levels, indicating that H<SUB>2</SUB>S and H<SUB>2</SUB>S<SUB>n</SUB> may play a significant role in the pathogenesis of PD. This result may be useful to biomedical studies, including PD.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A two-photon excitable and emission ratiometric probe for hydrogen polysulfide (H<SUB>2</SUB>S<SUB>n</SUB>) in mitochondria was developed. </LI> <LI> The probe is able to direct visualization of the endogenous H<SUB>2</SUB>S<SUB>n</SUB> level using two-photon microscopy (TPM). </LI> <LI> Ratiometric TPM imaging revealed that H<SUB>2</SUB>S<SUB>n</SUB> production is increased in a Parkinson’s Disease model. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

전통 사상 및 교육 방법의 현대 도덕 교육과정에서의 활용 가능성 연구

함규진 ( Kyu-jin Ham ),신창호 ( Chang Ho Shin ),임홍태 ( Hong Tae Lim ),지준호 ( Chun-ho Chi ) 한국철학사연구회 2016 한국 철학논집 Vol.0 No.50

도덕은 하나의 철학적, 심리적 상념으로서도 존재하지만, 실천으로 이어져야만 비로소 도덕으로서 역할을 할 수 있게 된다. 기본적으로 도덕이란 타자가 자신과 공존할 때, 그 타자와의 관계에서 좋음(goodness) 또는 옳음(righteousness)을 추구하는 것이기 때문이다. 그런 도덕이란 대부분 본능적으로 체득할 수 없기에 도덕교육이 있게 되는데, 한국인의 경우 서구와 다른 도덕적 전통을 갖고 있으며 따라서 서구적 도덕교육으로 충분하지 않거나, 전통적 도덕교육을 활용함으로써 더 나은 교육이 이루어질 수 있는 가능성을 찾을 수 있다. 그런 견지에서 전통의 실천적 의미를 재해석하고 활용하는 일은 한편의 논문으로는 너무 방대한 과제이다. 그래서 이 글에서는 비교와 범주화, 그리고 활용의 편의를 위해 전통을 ‘근세 유교(조선왕조 이후의 유교) 전통’으로 한정짓고, 다시 도덕교육 가운데 현행 도덕과 교육의 교육과정을 전통 교육과정과 비교함으로써 일정한 성과를 얻고자 하였다. 전통 교육과정은 기(氣)의 발달에 근거하여 연령별로 단계별 학습을 시도했으며, 그 핵심 이념은 인(仁)과 의(義), 실천적 덕목은 효(孝), 제(弟), 자(慈), 직(直), 근(勤), 검(儉)이라고 정리해볼 수 있다. 여기서는 그러한 전통 교육 이념이 ‘자신과의 관계’, ‘타인과의 관계’, ‘사회 공동체와의 관계’, ‘자연·초월과의 관계’로 영역을 나누고 각기 지향할 가치, 실천할 덕목을 배정한 현 도덕과 교육의 체계와 상통할 수 있다고 보고, 그에 따라 교육 현장에서 실천할 몇 가지 전통-현대 융합적 교육 방안들을 제안하였다. Any moral ideas get integrity upon their practices. Because in human society systems, morality have capability among human interactions, for it gives moral standards(good or bad/ right or wrong) to them. And when the society system is Korean, Koreans surely have developed unique styles of moral practice and moral education in their traditional ages. So reinterpretating and categorizing the moral tradition for taking advantage of it in contemporary context, make sense. In this study, Korean moral tradition is focused in its scope, as ``Confucian moral tradition in semi-modern era``. For convenience of study and the potentials of effective revision, Korean tradition have been resized. In Confucian moral tradition, semi-modern Korean moral ideas could be presented as ren(仁) and yi(義). Furthermore, ren and yi could be practiced with several moral virtues, like xiao(孝), di(弟), ci(慈), zhi(直) qin(勤) gian(儉). When compared with contemporary moral education idea systems, the traditional system can have affinity with the systems in moral ideas division(``with-self morality``, ``with-others morality``, ``with-universe and transcendence morality``). And several fusion-style, integration-based education practices can be developed and applied at contemporary school moral education.

축냉재 포접화합물의 열물성에 관한 실험적 연구 (IV) : 온도 변화 Changing of Temperature

고광옥,임재경,김창오,김진홍,정낙규,하옥남 조선대학교 에너지.자원신기술연구소 2000 에너지·자원신기술연구소 논문지 Vol.22 No.2

The objective of this paper is to investingate the thermal properties of TMA-clathrate applicable to cold storage system for building air-conditioning in order to use energy reasonably. Especially, the test tube experiments are performed by comparing and to analyzing the temperature of phase change, specific heat and subcooling characteristic according to the variation of concentrations and temperature of heat source in TMA clathrate. The result are summarized as follows; 1) temperature of phase change is dropped as the temperature of heat source is lower 2) specific heat of TMA-clathrate is about 24~95 % of pure-water 3) subcooling is smalled as the temperature of heat source is lower. Thus, it apears that TMA-clathrate has thermal properties as the cold storage medium for residential air-conditioning

Diagnostic Significance of Brainstem Auditory Evoked Potentials in Microvascular Decompression of Patients with Hemifacial Spasm or Trigeminal Neuralgia

Park, Sang-Koo,Lim, Sung-Hyuk,Park, Chan-Woo,Park, Jin-Woo,Chang, Sung-Ho,Park, Keun-Hye,Park, Hae-Ja,Song, Ji-Hye,Uhm, Dong-Ok,Kim, Ki-Bong 대한임상검사과학회 2011 대한임상검사과학회지(KJCLS) Vol.43 No.1

The purpose of this study was to analyse brainstem auditory evoked potentials (BAEP) wave change data during microvascular decompression (MVD). The nerve function of Cranial Nerve VIII is at risk during MVD. Intraoperative monitoring of BAEP can be a useful tool to decrease the danger of hearing loss. Between January and December 2009, 242 patients had MVD for hemifacial spasm (HFS) and trigeminal neuralgia (TN). Among intraoperative BAEP changes, amplitude of V-V' was the most frequently observed during cerebellar retraction and decompression step of the MVD procedure. 138 patients (57%) had no BAEP change while 104 patients (42.98%) had BAEP change. 69 patients (28.5%) had Type A-I, 16 patients (6.6%) had Type A-II, 5 patients (2.1%) had Type B, and 13 patients (5.37%) had Type C. MVD is a surgical procedure to relieve the symptoms (e.g. pain, muscle twitching) caused by compression of a nerve by an artery or vein. During BAEP intraoperative monitoring, the surgical step is important in interpreting the changes of wave V. Several potential mechanisms of injury may affect the cochlear nerve, and complete loss of BAEP is often associated with postoperative hearing loss. Intraoperative BAEP monitoring may provide an early warning of hearing disturbance after MVD.

수술 전 이하선 종괴의 위치파악에 이용하는 해부학적 경계표의 유용도

임치영(Chi Young Lim),김국진(Kook Jin Kim),임성주(Sung Ju Lim),이잔디(Jan dee Lee),남기현(Kee Hyun Nam),장항석(Hang Seok Chang),정웅윤(Woong Youn Chung),최홍식(Hong Shik Choi),박정수(Cheong Soo Park) 대한두경부종양학회 2006 대한두경부 종양학회지 Vol.22 No.1

Background: The location of parotid gland tumors can influence the duration and the difficulty of the operation. If the information about tumor location was available preoperatively, it would allow accurate operative planning and counseling of patients in terms of the length of the operation and the potential morbidity. Methods: This study was based on a retrospective review of 100 patients with parotid gland tumors underwent parotidectomy from January 2000 to October 2005 at Yong-Dong Severance Hospital. Based on computed tomographic(CT) scan findings, 4 landmarks such as facial nerve(FN) line, Utrecht(U) line, Conn's are(CA), and retromandibular vein (RV) were drawn on the scans in same plane. The location of tumors were determined by the landmarks and confirmed by the operative findings. The accuracy of each landmarks was evaluated. To find out the accuracies according to tumor size, the tumors were divided into 2 groups; less than 2 cm and larger than 2 cm in diameter. Results: U line was the most accurate(94%), sensitive(89.3%) and specific(97.7%) in predicting tumor location of the parotid gland. However, in small tumors less than 2cm, FN line (p=0.022) and RV criteria (p=0.028) were more reliable in accuracy. Conclusion: CA, FN line, U line, and RV are all useful landmarks in preoperative prediction for the location of parotid gland tumors. However, U line was the most accurate, but we must consider that proper landmark should be used in prediction according to the size of tumor because the accuracy of landmark may change.