승마 추출물의 항알레르기 효과

신태용(Tae Yong Shin),서형만(Hyung Man Seo),채병숙(Byeong Suk Chae) 대한약학회 1998 약학회지 Vol.42 No.4

Effects of the aqueous extract of Cimicifuga heracleifolia (CHAE) on the allergic reactions were investigated. CHAE inhibited systemic anaphylaxis induced by compound 48/80 in mice dose-dependently. Especially, CHAE inhibited compound 48/80-induced systemic anaphylaxis 100% with a dose of 0.5mg/g body weight. CHAE significantly inhibited serum histamine levels induced by compound 48/80. CHAE inhibited histamine release from the rat peritonea] mast cells activated by compound 48/80 or anti-DNP IgE. Our studies provide evidence that CHAE will be beneficial in the treatment of anaphylias.

Random Circuit Breaker Network Model for Unipolar Resistance Switching

Chae, Seung Chul,Lee, Jae Sung,Kim, Sejin,Lee, Shin Buhm,Chang, Seo Hyoung,Liu, Chunli,Kahng, Byungnam,Shin, Hyunjung,Kim, Dong-Wook,Jung, Chang Uk,Seo, Sunae,Lee, Myoung-Jae,Noh, Tae Won WILEY-VCH Verlag 2008 ADVANCED MATERIALS Vol.20 No.6

<B>Graphic Abstract</B> <P>The random circuit breaker network model is proposed for unipolar resistance switching behavior. This model describes reversible dynamic processes involving two quasi-metastable states. The formation and rupture of conducting channels (see figure) in the polycrystalline TiO<SUB>2</SUB> thin films may be analyzed by the self organized avalanche process in the random circuit breaker network model. <img src='wiley_img/09359648-2008-20-6-ADMA200702024-content.gif' alt='wiley_img/09359648-2008-20-6-ADMA200702024-content'> </P>

Utilization of Evaporation during the Crystallization Process: Self-Templation of Organic Parallelogrammatic Pipes

Seo, Myungeun,Kim, Jung Hak,Seo, Gon,Shin, Chae-Ho,Kim, Sang Youl WILEY-VCH Verlag 2009 Chemistry Vol.15 No.3

<P>Analogues of 4-dodecyloxy-2-trifluoromethylbenzamide (12FH2) consisting of a hydrophobic alkyl chain, a trifluoromethylated aromatic ring, and a self-complementary hydrogen-bonding amido group were synthesized, and the structural effect of each component on the formation of parallelogrammatic pipes was investigated. Differential scanning calorimetry and powder XRD analyses revealed that all-trans L and gauche-rich S polymorphic forms appeared for the analogues with more than eight carbon atoms in the alkyl chain, that is, the polymorphism originates in the conformation of the alkyl groups and hydrogen-bonding patterns of the benzamide group. Also, the trifluoromethyl substituent is crucial in that it provides an appropriate molecular balance between the benzamide and alkyl groups. Scanning electron microscopy and powder XRD analyses of solids obtained by a drying-mediated assembly process revealed that production of the L polymorph by polymorphic transition from the S polymorph resulted in evolution of a three-dimensional structure when the alkyl group has more than 12 carbon atoms. Among the series of compounds, 12FH2 and 4-tetradecyloxy-2-trifluoromethylbenzamide (14FH2) formed parallelogrammatic pipes with micrometer dimensions. An atomic force microscopy study of 12FH2 suggested that a single pipe may be composed of platelike crystallites of L polymorph. From a mercury-intrusion porosimetry study, it was determined that macroporous materials with average pore diameters of about 40 μm and porosity of about 80 % were obtained. The previously proposed self-templation mechanism by polymorphic transition from S to L polymorph was further discussed in view of polymorphism and the crystallization rate. An appropriate molecular balance between the benzamide and alkyl groups is necessary to induce a proper polymorphic transition for the development of a three-dimensional hollow structure in the evaporation process.</P> <B>Graphic Abstract</B> <P>Hollow micropipes with parallelogrammatic shape can be obtained by simple evaporation of a solution of C<SUB>12</SUB> or C<SUB>14</SUB> alkoxy-2-trifluoromethylbenzamide on a substrate. A study on analogues with various alkyl chain lengths revealed that an evaporation-induced self-templated polymorphic transition, in which one polymorph acts as a template and the other as a negative replica, is responsible for the formation of hollow three-dimensional crystalline structures (see picture for schematic mechanism and SEM image of a pipe). <img src='wiley_img/09476539-2009-15-3-CHEM200801408-content.gif' alt='wiley_img/09476539-2009-15-3-CHEM200801408-content'> </P>

Scientific Publications on Thyroid Ultrasound between 2001 and 2020: Differences in Research Characteristics by Disciplines

Shin Won Chul,Lee Chae Woon,Ha Jiyeon,Lim Kyoung Ja,Seo Young Lan,Yun Eun Joo,Yoon Dae Young 대한영상의학회 2022 Korean Journal of Radiology Vol.23 No.8

Objective: To analyze the characteristics and trends of scientific publications on thyroid ultrasound (US) from 2001 to 2020, specifically examining the differences among disciplines. Materials and Methods: The MEDLINE database was searched for scientific articles on thyroid US published between 2001 and 2020 using the PubMed online service. The evaluated parameters included year of publication, type of document, topic, funding, first author’s specialty, journal name, subject category, impact factor, and quartile ranking of the publishing journal, country, and language. Relationships between the first author’s specialty (radiology, internal medicine, surgery, otorhinolaryngology, and miscellaneous) and other parameters were analyzed. Results: A total of 2917 thyroid US publications were published between 2001 and 2020, which followed an exponential growth pattern, with an annual growth rate of 11.6%. Radiology produced the most publications (n = 1290, 44.2%), followed by internal medicine (n = 716, 24.5%), surgery (n = 409, 14.0%), and otorhinolaryngology (n = 171, 5.9%). Otorhinolaryngology and internal medicine published significantly more case reports than radiology (p < 0.001, each). Radiology published a significantly higher proportion of publications on imaging diagnosis (p < 0.001 for all) and a significantly lower proportion of publications on biopsy (p < 0.001 for all) than the other disciplines. Publications produced by radiology authors were less frequently published in Q1 journals than those from other disciplines (p < 0.005 for internal medicine and miscellaneous disciplines and < 0.01 for surgery and otorhinolaryngology). China contributed the greatest number of publications (n = 622, 21.3%), followed by South Korea (n = 478, 16.4%) and the United States (n = 468, 16.0%). Conclusion: Radiology produced the most publications for thyroid US than any other discipline. Radiology authors published more notably on imaging diagnosis compared to other topics and in journals with lower impact factors compared to authors in other disciplines.

Biological Activities of Sweet Potato Tips and Tuber

Chae Young Hong,Jeong Hyun Seo,Tae Yong You,Yeon Jae Jo,Tae Hwan Shin,Tea Su Kang,Heon Sang Jeong 한국식품영양과학회 2020 한국식품영양과학회 학술대회발표집 Vol.2020 No.10

Derivation of primitive neural stem cells from human‐induced pluripotent stem cells

Shin, Woo Jung,Seo, Ji‐,Hye,Choi, Hyun Woo,Hong, Yean Ju,Lee, Won Ji,Chae, Jung Il,Kim, Sung Joo,Lee, Jeong Woong,Hong, Kwonho,Song, Hyuk,Park, Chankyu,Do, Jeong Tae John WileySons, Inc. 2019 Journal of comparative neurology Vol.527 No.18

<P><B>Abstract</B></P><P>Human‐induced pluripotent stem cells (hiPSCs) have facilitated studies on organ development and differentiation into specific lineages in in vitro systems. Although numerous studies have focused on cellular differentiation into neural lineage using hPSCs, most studies have initially evaluated embryoid body (EB) formation, eventually yielding terminally differentiated neurons with limited proliferation potential. This study aimed to establish human primitive neural stem cells (pNSCs) from exogene‐free hiPSCs without EB formation. To derive pNSCs, we optimized N2B27 neural differentiation medium through supplementation of two inhibitors, CHIR99021 (GSK‐3 inhibitor) and PD0325901 (MEK inhibitor), and growth factors including basic fibroblast growth factor (bFGF) and human leukemia inhibitory factor (hLIF). Consequently, pNSCs were efficiently derived and cultured over a long term. pNSCs displayed differentiation potential into neurons, astrocytes, and oligodendrocytes. These early NSC types potentially promote the clinical application of hiPSCs to cure human neurological disorders.</P>

Aquatide Activation of SIRT1 Reduces Cellular Senescence through a SIRT1-FOXO1-Autophagy Axis

( Chae Jin Lim ),( Yong-moon Lee ),( Seung Goo Kang ),( Hyung W. Lim ),( Kyong-oh Shin ),( Se Kyoo Jeong ),( Yang Hoon Huh ),( Suin Choi ),( Myungho Kor ),( Ho Seong Seo ),( Byeong Deog Park ),( Keedo 한국응용약물학회 2017 Biomolecules & Therapeutics(구 응용약물학회지) Vol.25 No.5

Ultraviolet (UV) irradiation is a relevant environment factor to induce cellular senescence and photoaging. Both autophagy- and silent information regulator T1 (SIRT1)-dependent pathways are critical cellular processes of not only maintaining normal cellular functions, but also protecting cellular senescence in skin exposed to UV irradiation. In the present studies, we investigated whether modulation of autophagy induction using a novel synthetic SIRT1 activator, heptasodium hexacarboxymethyl dipeptide-12 (named as Aquatide), suppresses the UVB irradiation-induced skin aging. Treatment with Aquatide directly activates SIRT1 and stimulates autophagy induction in cultured human dermal fibroblasts. Next, we found that Aquatide-mediated activation of SIRT1 increases autophagy induction via deacetylation of forkhead box class O (FOXO) 1. Finally, UVB irradiation-induced cellular senescence measured by SA-β-gal staining was significantly decreased in cells treated with Aquatide in parallel to occurring SIRT1 activation-dependent autophagy. Together, Aquatide modulates autophagy through SIRT1 activation, contributing to suppression of skin aging caused by UV irradiation.

인체 간암세포주(Hep 3B)의 증식에 미치는 amiloride의 억제효과

신채희,박기룡,최병주,서수홍,김성훈,박무인,박선자,정근옥,박건영,구자영 고신대학교 의학부 2002 高神大學校 醫學部 論文集 Vol.17 No.1

Background/Objective Cytoplasmic alkalinization induced by activation of the Na+/H+antiporter which is stimulated upon the addition of growth-promting agents, such as insulin, epidermal growth factor, phorbol ester, plays an essential role in the initiation on cell proliferation. In the present study the effects of amiloride, a specific and reversible inhibitor of Na+/H+exchanger, on the growth of human hepatocellular carcinoma cell line, Hep 3B were examined and the effects of 5-fluorouracil (5-FU) combined with amiloride were also studied to determine the role of amiloride in the treatment of hepatocellular carcinoma. Cell cycle analysis was done to examine the mechanisms for the inhibitory effects of amiloride. Methods The growth of Hep 3B cells were examined by counting cell number on two and four days treatment with 1μM, 10μM, 20μM, 40μM, 80μM, 160μM, amiloride, and 0.1㎍/㎖, 0.3㎍/㎖ 5-FU, after plating Hep 3B cells into 35-mm^(2) plastic dishes at a density of 20×10^(4) cells/dish. The reversibility of the effects of amiloride was examined on two days to eight days treatment with 20μM amiloride after seeding 4×10^(4) cells/dish Cell cycle analysis was done on the cells after four days treatment with 20μM amiloride. Results Amiloride significantly inhibited the growth of Hep 3B in a dose-dependent fashion (p<0.05). The inhibitary effect of amilride on the growth of Hep 3B cells was firstly shown at the concentration of 1μM, which is not so higher than the concentration of 0.1-0.2μM attainable by administration of usual dose of amiloride (5∼10㎎). Forty-eight percent inhibition of growth was found at an amiloride concentration of 20μM and 92% inhibition of growth was found at an amiloride concentration of 160μM after 4days treatment. The removal of amiloride by a media change after 48 hours treatment lead to significantly more growth than amiloride treated group (p<0.05). Amiloride combined with 5-FU significantly inhibited the growth of Hep 3B in a dose-dependent fashion compared to an amiloride or a 5-FU of cells in G0-G1 phase, S phase and G2-M phase was 57.9%, 20.8%, 21.3%, respectively in the amiloride group (20μM) and 38.9%, 20.8%, 40.3% in the control group, showing much higher G1 fraction in amiloride group compared to control group. Conclusions Amiloride significantly inhibited the growth of Hep 3B in dose-dependent fashion, which may be reversible. The reversibility of growth inhibition suggests that amiloride is not a non-specific cytotoxin for Hep 3B cells. Because the lowest inhibitory concentration of amiloride for the growth of Hep 3B cells in this study was 1μM, which is not so higher than the concentration of 0.1∼0.2 μM attainable by administration of usual dose of amiloride(5∼10㎎). amiloride or its analogues may be used alone or in conjunction with other modalities of therapy of hepatocellular carcinoma, for example, hepatic arterial chemoembolization of radiofrequency interstitial thermal ablation therapy. Since Hep 3B cell transition through the G1 phase was inhibited by amailoride, the inhibitory effects of amiloride on the growth of Hep 3B may be mediated in part by blocking G1-S transition. Further study is needed to clarify the effects of more potent analogues of amiloride on the growth of human hepatocellular carcinoma.

Multi-layer Ceramic-Pillar Composite Structures for Piezoelectric Energy Harvesters

Shin, D.-J.,Chae, M.-S.,Koh, J.-H.,Cho, K.-H.,Seo, C.-E. Taylor Francis 2014 Ferroelectrics Vol.466 No.-

<P>In this paper, we will discuss piezoelectric energy harvesters of multi-layered ceramic-pillar composite structures to be used as the energy source of small size electronic devices. 0.2(PbMg1/3Nb2/3O3)-0.8(PbZr0.475Ti0.525O3) ceramics and polydi-methylsiloxane (PDMS) were employed as piezoelectric ceramic-pillar composite, which prepared in forms of rectangular pillar structures. The piezoelectric ceramic-pillar composites were sintered separately and attached on the metallic bottom electrode with poled states. Symmetric upper electrodes were attached on the other side. Subsequently, the ceramic-pillar structures of composite material were manufactured with single-layer, double-layer and triple-layer devices. Both of multi-layer and single layer devices will be compared with several analysis methods. Piezoelectric properties will be discussed including the piezoelectric constant. Also, the output power of the multi-layer and single layer devices will be studied.</P>

Repression of PPARγ Reduces the ABCG2-mediated Efflux Activity of M2 Macrophages

( Chae Eun Kim ),( Ha Young Park ),( Jae-gook Shin ),( Su-kil Seo ) 대한결핵 및 호흡기학회 2020 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.128 No.-

Background Even though subclasses of macrophage have distinct roles during progression of infectious diseases, it remains poorly understood whether there is a subset-specific difference in drug responses. Here, we report that ABCG2 was expressed specifically in M2-like macrophages and that it controlled their efflux activities. Methods Macrophage subsets (M1/M2-like macrophages) are generated from THP-1 cells and CD14+ monocytes by the nomenclature of in vitro. ABCG2 expression is analyzed by real-time PCR and flow cytometry. The intracellular concentration is measured using Hoechst 33342 efflux assay. The binding of PPARγ in the Abcg2 promoter region is measured using chromatin immunoprecipitation assay. Results Abcg2 expression is markedly induced during polarization of PMA-primed macrophages toward an M2 type. IL-4 and IL- 13 induced Pparg expression through STAT6 and PPARγ in turn acted on the Abcg2 promoter for its transcription activation. Once polarized to M2-like macrophages, these cells had sustained PPARγ transcription activation of Abcg2 gene. Accordingly, interruption of this machinery by T0070907, an inverse agonist of PPARγ, was shown to be effective in Abcg2 downregulation and its efflux activity in M2-like macrophages. Conclusions Our Results implicate that ABCG2 of M2 macrophages may function as an important pump that plays a potential role in drug efflux and that T0070907 may be used to increase the efficacy of M2 macrophage-targeting drugs such as antibiotics.