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Isolation and Transcriptional Expression of CuZn Superoxide Dismutase from Codonopsis lanceolata
Lee,Kang,In,Jun-Gyo,Yu,Chang-Yeon,Yun,Song-Joong,Min,Byung-Hoon,Rho,Yeong-Deok,Kim,Moo-Sung,Yang,Deok-Chun 한국자원식물학회 2004 Plant Resources Vol.7 No.3
To investigate the defense mechanism against the abiotic stress, a cDNA clone encoding a CuZn superoxide dismutase (CuZnSOD) protein was isolated from a cDNA library prepared from tabroot mRNAs of Codonopsis lanceolata. The eDNA, designated ClSODCc, is 799 nucleotides long and has an open reading frame of 459 bp with a deduced amino acid sequence of 152 residues. The deduced amino acid sequence of ClSODCc matched to the previously reported CuZnSODs. Consensus amino acid residues (His-45, -47, -62, -70, -79, -119 and Asp-82) were involved in Cu-, Cu/Zn-, and Zn- binding ligands. The deduced amino acid sequence of ClSODCc showed high homologies (82%-86%) regardless of species. Expression of ClSODCc by oxidative stress was increased up to 1 h after treatment and declined gradually. Much earlier and stronger expression of ClSODCc was observed in the cold stress treatment.
이진호,최선웅,강길선,노승무,민병무,김용백,김학용 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1
"Biomaterials" are nonviable materials used in medical devices, intended to interact with biological systems. They should have "biocompativility", which is defined as the ability of materials to perform with an appropriate host response in a specific application. It includes many materials that are often classified as biomaterials. Metals, ceramics, polymers, glasses, carbons, and composite materials are some examples. Among them, biodegradable polymers are discussed in this revies article. Since a biodegradable polymeric implant does not have to be removed surgically once it is no longer needed, biodegradable polymers are of value in short-term applications that require only the temporary presence of a polymeric implant. Main short-term medical applications of biodegradable polymers include the temporary tissue scaffold, the temporary adhesion barrier, the drug delivery device, and the multifunctional implant. In this article, the properties, processing methods, and medical applications of the biodegradable polymers are reviewed.
化學發癌 物質의 發癌 能力에 關한 反應性 指數의 理論的 硏究
李一峯,宋寧大,林周相,崔茂悅,朴柄珏 嶺南大學校 基礎科學硏究所 1990 基礎科學硏究 Vol.10 No.-
There are many theoretical indices for chemical carcinogenicity. Among the various indices, the LUMO electron density and approximate superdelocalizability have taken to compared and discussed for the carcinogenicity of PAHs, aromatic amines and Azo-compounds. The sum of LUMO electron density of the trans-butadiene type 1, 2, 4 positions in a molecule has been known to be in more agreement with the observed values than those of approximate superdelocalizability. It is suggested that any trans-butadiene type 1, 2, 4 positions play an importmant role in the elucidation of mechanism of chemical carcinogenesis.
이종흔,정동균,정태영,김각균,민병무,이공훈 대한구강생물학회 1980 International Journal of Oral Biology Vol.4 No.1
This experiment was performed to elucidate the reported anti-cariogenic effect of capsicum in rats. Streptococcus mutans strain B-13, which was drived from human oral cavity and known to be cariogenic, was cultured in TYG with water-extract of capsicum. Glucose content of water-extract of capsicum was also determined by glucose oxidase method. The results are as follows. 1. Glucose content of water-extract of capsicum was 25.6 (±0.5) %. 2. In logarithmic phase, water-extract of capsicum group showed less steepness in growth curve than control group. 3. In early stationary phase, size of bacterial cell mass was in order of control, 0.5% and 1% water-extract of capsicum group. 4. It appears that water-extract capsicum might contain inhibitory substance to growth of Streptococcus mutans B-13, in spite of its high glucose content.
위암 환자의 복강내 투여를 위한 Activated Charcoal-Alginate Bead 제형으로부터 Mitomycin C의 용출 거동
이진호,최선웅,서중기,김동민,정경수,오정연,김진향,노승무,민병무,김용백,김창식,박근성,강대영,송규상,양준묵,조준식,정현용,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1
Locoregional recurrence is the most common type of recurrence in surgical operation of gastric adenocarcinoma, and peritoneal dissemination is one of the most difficult problems in advanced gastric adenocarcinoma treatment. Because the peritoneal cavity is the most common site of the first recurrence after gastric cancer resection, intraperitoneal chemotherapy seems a logical choice for cancer chemotherapy. In this study, Mitomycin C (MMC)-activated charcoal (CH)-alginate (ALG) beads were prepared by the mixtures of CH particles adsorbed with MMC as an anti-cancer drug and aqueous alginate solution. The alginate is recognized as biodegradable, nontoxic, and biocompatible. The release of MMC from the beads in 0.1 M Tris buffer was stable and continuous until about 1 week. The MMC-CH-ALG beads can be applied in the peritoneal cavity for intraperitoneal chemotherapy since they provide a good adhesiveness on the tissue and controlled release pattern of the drugs.
이종흔,정태영,김중수,이효재,민병무,정동균 대한구강생물학회 1980 International Journal of Oral Biology Vol.4 No.1
These studies were performed in an effort to elucidate the relation between dental fluorosis and several minerals which are contained in drinking water in the regions of the high fluorosis index. The water samples were collected from several regions around the Mankyung River in Chonrabukdo where high fluorosis index was observed in the pupils and from the control regions where dental fluorosis were not reported. The contents were analyzed by means of spectrophotometry for phosphorus, fluoride ion activity for fluoride, and atomic absorption spectrophotometry for calcium, magnesium and manganese. The calcium concentrations of drinking waters collected from the regions where high degree of dental fluorosis was appeared were lower than those of control regions. The phosphorus concentrations in the regions of high fluorosis index were very high, and the fluoride concentrations were remarkably greater than those of control regions. There was regional change in the magnesium concentrations of drinking water collected from the regions of the high dental fluorosis and control regions. But manganese concentrations showed markedly high regional variation.
李茂錫,朴吉文,曺秉崎 조선대학교 생산기술연구소 1984 生産技術硏究 Vol.6 No.2
The pressure props of coiled tubes were studied experimentally with hydraulic oil Gulf Harmony 32 for unsteady flows. In addition, friction factors are investigated as function of Curvature ratio(a/R), Reynolds number (Re), Dean number(De), and frequency parameter(α). The three coils were used with curvature ratio (a/R) of O.1, O.04, and O.02, and the total length of coiled tube is 11.3m, respectively. The experimental results are obtained as follows; 1). Pressure drops of straight tubes agree with Hagen-Poiseuille fomula. 2). Ratio of friction factors (fc/fs) is more than 1, and increases with Reynolds number. 3). Friction factor increases as curvature ratio (a/R) and number of coil are higher. 4). The ratio of friction factor (fc/fs) is higher when frequency parameter is of about 2∼3.