扶正抗癌湯의 抗腫瘍效果에 關한 實驗的 硏究

文錫哉,文九,金柄住,田炳薰,元秦喜 대한동의병리학회 1997 동의생리병리학회지 Vol.11 No.2

腫瘍으로 인한 死亡率은 多樣한 名種 治療에도 不拘하고 循環器疾患 다음으로 2위를 차지하고 있다. 이에 著者는 韓醫學과 西醫學의 結合治療를 通해서 抗腫瘍藥物의 效果를 增進시키고 副作用을 줄이는 藥物의 開發을 爲하여 健脾 益氣 祛痰 補腎 杭癌效能을 갖고 잇는 扶正杭癌湯抽出液을 使用하여 Colony 形成抑制實驗, Sulforhoda-mine B(SRB) assay를 觀察하였고, 扶正抗癌湯抽出液과 抗癌化學療法劑인 Mitomycin C(MMC)를 Ehrlich carcinoma의 solid tumor와 ascites tumor에 各各 單獨 및 倂用 投與하여 抗腫瘍效果 및 Iysosomal enzymes의 活性에 대한 效果를 觀察하였다. 먼저 扶正抗癌湯抽出液을 Caki-1 cell, Hep 3B 및 A549에 투여한 후 增殖抑制作用을 colony 形成抑制實驗과 SRB assay를 통하여 觀察한 결과 濃度依存的으로 腫瘍細胞의 成長을 抑制하였다. Ascites form of Ehrlich carcinoma에 대한 抗腫瘍效果 實驗에서 扶正抗癌湯抽出液과 MMC를 병용투여한 결과, 扶正抗癌湯抽出液에 의하여 MMC의 抗腫瘍效果가 增加하였다. Solid form of Ehrlich carcinoma에 대한 抗腫瘍效果 實驗에서 扶正抗癌湯抽出液과 MMC를 병용 투여하였을 경우, MMC를 단독 투여하였을 경우보다 腫瘍의 크기가 현저하게 減少하는 결과를 보였다. Ehrlich ascites carcinoma cell에 扶正抗癌湯抽出液과 MMC를 병용 투여하였을 경우, MMC를 단독 투여하였을 경우보다 lysosomal enzymes의 活性이 强하게 나타났다. 本 實驗을 통하여 扶正抗癌湯抽出液은 MMC의 抗腫瘍效果를 증가시키는 效果를 보였다. 또한 扶正抗癌湯抽出液은 마우스에서 solid form of Ehrlich ascites carcinoma의 크기를 감소시키는 效果를 나타냈으며, 생존기간 實驗에서는 扶正抗癌湯抽出液과 MMC를 병용투여시 ascites tumor에 MMC를 단독으로 투여하였을 때와 비교하여 유의하게 생존기간을 증가시키는 결과를 보였다. 이상의 결과를 종합하여 볼 때 扶正抗癌湯抽出液은 用量에 依存的으로 直接的인 抗腫瘍效果가 있으며, 抗癌療法劑인 MMC의 效果를 亢進시키는 것으로 보아 間接的인 抗腫瘍效果도 있을 것으로 思科되나 正確한 效果를 究明하기 위해서는 보다 正確한 抗腫瘍機轉에 대한 硏究와 藥物學的 硏究 및 臨床的 硏究가 追加되어야 할 것이다. In order to investigate the effects of Bujeonghangamtang Extract(扶正抗癌湯抽出液) on antitumor effects after human cell lines(A549, Hep3B, Caki-1, Ehrlich) transplantation into the peritoneal cavity or right groin in mice induced by RPMI 1640 and DMEM etc, the extracts of its herbal medicines were orally administered for 10 or 12 days. Experimental studies were performed for measurement of antitumor effect of Mitomycin C(MMC) and lysosomal enzyme's activities using colony forming efficiency, SRB assay which were regarded as a valuable method for the measurement of antitumor effects of unknown compound on tumor cell lines. The results obtained in this studies were as follows: 1. The change of colony-forming efficiency and SRB assay of Caki-1 cells, Hep3B and A549 Cells after exposure to the extract of Bujeonghangamtang extract depressed the growth of tumor cells by concentration of Bujeonghangamtang. 2. Antitumor activity of the ethanol extract of Bujeonghangamtang extract and MMC on ascites form of Ehrlich carcinoma in mice is slightly improved. Especially the mean of survival times in the group of 200㎎/㎏ and MMC 0.1㎎/㎏ is improved over 30.9%. 3. When Bujeonghangamtang extract and MMC are administered together, the weight of tumor is more decreased than MMC alone. 4. The lysosomal enzyme's activities of the Bujeonghangamtang extract and MMC are more significantly improved than MMC alone. According to the above result, it could be suggested that Bujeonghangamtang extract has indirect antitumor effect by the increase of MMC uptake.

딸기 시들음병균에 대한 Trichoderma 속 균의 길항작용에 관한 연구 : Ⅳ. Antagonism of T. harzianum in Soils Ⅳ. 토양에서의 T.harzianum 길항작용

문병주,정후섭,조종택 東亞大學校生命資源科學大學附設 農業資源硏究所 1994 農業生命資援硏究 Vol.3 No.2

In vitro에서 볏짚, 밀기울, 옥수수분말, chitin 또는 게껍질을 첨가하면 T. harzianum의 딸기 시들음병균에 대한 길항작용은 높았으나 clover 잎 분말에서는 낮았다. 또한 이 길항작용은 25∼30℃, 산성토양(pH 3.5-5.5), 40∼50%의 토양수분에서 촉진되었다. When rice straw, wheat bran, corn, chitin or crabshell powder was added in soil in vitro, the antagonistic activity of T. harzianum on F. oxysporum f. sp. fragariae increased, whereas incorporation of clover leaf powder had no effect on the antagonistic activity. The activity was also the highest at 25∼30℃ and in acid soil(pH 3.5∼5.5) with 40∼50% moisture content.

Fractal 차원에 의한 표면경화면과 마찰면의 형태해석

문병주,조연상,옥철호,박흥식,전태옥 동아대학교 생산기술연구소 2001 生産技術硏究所硏究論文集 Vol.6 No.1

The determination of surface middleography is believed to be extremely important in the areas of contact mechanics, adhesion and friction. In order to describe middleography of various rubbed and hardened surface, the wear test was carried out under different experimental conditions in dry friction. And fractal descriptors was applied to rubbed and hardened surface of laser modified steel with image processing system. These descriptors to analyze surface structure are fractal dimension. Surface fractal dimension can be determined by sum of intensity difference of surface pixel. middleography of rubbed and hardened surface can be effectively obtained by fractal dimensions.

백서에서 Depulpin®과 Formocresol에 대한 치수와 치근단 조직의 반응

문형인,김선호,황윤찬,오병주,황인남,김선헌,정선와,윤창,오원만 大韓齒科保存學會 2002 Restorative Dentistry & Endodontics Vol.27 No.4

One fifth dilution of formocresol is usually for pulpotomy of the primary teeth and emergency pulpotomy of the permanent teeth. However, the use of formaldehyde has been subjected to criticism because it may be absorbed into the blood stream and become distributed systemically, it may also alter the pulp tissue rendering it immumologically active, and have carcinogenic potential. Recently Depulpin®(VoCo., Germany) gains popularity as a devitalizing agent during root canal therapy in spite of high concentration of 49% paraformaldehyde because it facilitate devitalization of pulp and make root canal therapy easier. But there have been not enough publications about the reaction of pulp and periapical tissue caused by Depulpin. This study was performed to evaluate the histological changes in pulp and periapical tissue of rats after pulpotomy using formocresol and Depulpin and to elucidate the toxic effects of these agents. Thirty six Sprague-Dawley rats were anesthetized by intraperitoneal injection of ketamine. Maxillary first molar teeth were used for pulpotomy with formocresol and Depulpin. Rats were sacrificed after 2 days, 4 days, 1 week, 2 weeks, 3 weeks and 4 weeks respectively. Specimens were histologically observed by light microscope changes in pulp and periapical tissue. The obtained results were as follows. 1. Formocresol group A zone of fixed tissue, in which odontoblasts could clearly be defined, was present directly underneath the pulpotomy dressing in almost all teeth of this group. This was followed by an area of necrotic tissue which resembled dried out fibrous tissue with no cellular detail except some pyknotic nuclei. In the specimens of after 2 days, 4 days, 1 week, 2 weeks in which vital tissue was present. it was separated from the fibrous area by a zone of inflammation. In the specimens of after 3 weeks and after 4 weeks, inflammatory infiltrate was in the periodontal ligament adjacent to the apical foramina of the teeth. 2. Depulpin® group The area of necrotic tissue which had no cells and fibers , was present adjacent to the dressing. This was followed by dried out fibrous tissue with no cellular details except some pyknotic nucleli. A short stump of vital pulp with odontoblasts was present at the end of the canal after 2 days. Inflammatory infiltrate was in the periodontal ligament after 4 days and after 1week. Severe root resorption and necrosis of periapical tissue opposite the root resorption site were defined after 2 weeks and after 3 weeks. Periapical lesion which consist of necrotic tissue surrounded by a fibrous connective wall. was found after 4 weeks. The results indicated that Depulpin can cause more adverse reaction to the dental pulp and periapical tissue than formocresol, and further studies are needed for its clinical use with safety.

카아네이션 잎마름병 (Alternaria dianthi Stevens & Hall)의 발생에 관하여

文炳周,孫賢秀,金正基 東亞大學校 大學院 1984 大學院論文集 Vol.8 No.2

The study has been carried to examine a new disease of carnation caused by Alternaria isolate, which was found in Gim Hae, Gyoungnam province and the suburbs of Busan City, recently. 1. The pathogen was identified as Alternaria dianthi Stevens & Hall which has not been described in Korea. The common name of the disease was given as IB Ma Reum Byung(carnation blight). 2. Morphological studies of the fungus have shown a wide variation in shape, size and septation of conidia. The size of conidia formed on carnation leaf lesions in field is 22.8-101.2μ(av. 60.4μ)×10.1-27.8μ(av. 18.6μ), which was larger than those on potato dextrose agar in vitro. 3. Of 13 carnation varieties tested, Boston, Linda and Red Alaska were highly susceptible, and Angel, Flamingo Sim, William Sim and Yo Soo were moderately susceptible whereas Brauns Yollw Sim, Colquate, Imp Sisney Little Field, Cardinal Sim, La Reve Salmon Sim and Safari were slightly susceptible to the fungus under the condition of artificial infection.

딸기 시들음병균에 대한 Trichoderma 속 균의 길항작용에 관한 연구 : Ⅲ. Chitinase and β-1,3-glucanase Activity Ⅲ. Chitinase와 β-1,3-glucanase 활성

문병주,정후섭,조종택 東亞大學校生命資源科學大學附設 農業資源硏究所 1994 農業生命資援硏究 Vol.3 No.1

T. harzianum은 합성 배지에 유일한 탄소원으로서 chitin, laminarin, 본 병균의 세포벽, 밑기울 분말, clover잎 분말, maltose를 단독으로 첨가하여 생육시키면 chitinase와 β-1, 3-glucanase를 생성하였으나, chitinase 활성은 세포벽 첨가구에서 가장 높았고 다음은 chitin이었으며 maltose와 밑기울에서도 높은 활성을 보였으나 clover에서는 극히 낮았다. β-1, 3-glucanase 활성은 maltose에서 가장 높았고 다음은 laminarin이었으며 그 이외에는 극히 낮았다. 양 효소의 활성은 pH 3.5와 5.5가 pH 7.5에 비하여 높았으며, T. harzianum과 본 병균을 혼합하면 단독배양에 비하여 높았다. T. viride도 유일한 탄소원으로서 chitin 또는 laminarin을 첨가하면 각각 chitinase와 β-1,3-glucanase를 생성하였으나, chitinase 활성은 T. harzianum에 비하여 현저하게 낮았고 β-1,3-glucanase의 활성은 비슷하였다. Chitinase production by Trichoderma harzianum was highest in the culture with cell walls of the pathogen or chitin, moderately high with maltose or wheat bran, and very poor with clover leaf power. Relatively large amount of β-1,3-glucanase was excreted by T. harzianum when maltose or laminarin was used as a sole carbon source for the culture of the fungus, whereas cell walls, wheat bran and clover leaf powder had little effect on the enzyme production. For both of the enzymes, the enzyme activities were higher at pH 3.5-5.5 than at pH 7.5, and higher enzyme activities were detected in the dual culture of T. harzianum and Fusarium oxysporum f. sp. fragariae than in the culture of T. harzianum alone. The activity of chitinase by T. viride was significantly lower than by T. harzianum, and the activity of β-1,3-glucanase was similar between the two antagonisitcs, although T. viride produced chitinase and β-1,3-glucanase when chitin or laminarin was added as a sole carbon source.

二面交雜에 의한 무(Raphanus sativus l.)의 收量 形質들에 대한 量的遺傳 分析

황주광,전병문,조대환 충북대학교 농업과학기술연구소 2001 農業科學硏究 Vol.18 No.-

Eight parents and a set of 28 diallel crosses were employed to investigate heterosis, combining ability and inheritance of yield characters in radish(Raphanus sativus L.). Using model 1 of Griffing's method 2, heterosis and combining abilities of above characters were estimated. With Jinks and Hayman's method, genetic variance component analysis were performed. The results obtained are summarized as follows. 1.Heterosis was highest for root weight(156.0%). Characters such as leaf weight (55.5%), root length(45.9%), root diameter of middle portion(39.2%), root diameter of lower portion(37.3%) and root diameter of upper portion(32.3%) showed positive heterosis, while T/R ratio(-41.9%) showed negative heterosis. 2.In the analysis of combining ability, mean squares of GCA and SCA were highly significant for the all characters investigated, except leaf length character. 3.The significant positive GCA effects for root length, root weight, number of leaves and root shape index were shown in Tokinashi and Minowase. Yonghyeon and Jungkukcheongpi showed the significant positive GCA effects for root diameter, root weight and leaf length. However, Jinjudaepyeong and Wulsanjaerae showed a negative GCA effect for root length and root weight and, especially Taimayhua showed a negative GCA effect for all characters investigated. 4.The combinations of Tokinashi x Taimayhua, Tokinashi x Wulsanjaerae and Jinjudaepyeong x Minowase indicated the significant positive SCA effects for root length, root diameter and leaf weight. 5.In the degree of dominance, root length, root diameter, T/R ratio, number of leaves and root shape index demonstrated partial dominance, while leaf length showed overdominance. 6.Dominance effects of genes were greater than additive ones for root length, root diameter and leaf weight. Number of leaves, T/R ratio and root shape index expressed larger additive effects. But root weight and leaf length showed overdominance. 7.Heritabilities in broad sense(h2B) were very high for all characters investigated. Heritabilities in narrow sense (h2N) were highs for root length, number of leaves and root shape index, but low for leaf length.

새로운 알랄아민 항진균제의 합성과 생물학적 평가

정병호,박은주,문현주,유진철 朝鮮大學校 1997 藥學硏究誌 Vol.18 No.2

Some allylamine compounds which are benzothiazole substituants in stead of naphthylring in naftifine, antifungal agents, were sythesized as potential antimycotics. The intermediate Schiff bases that were obtained by condensation of 2-aminobenzothiazole and trans-cinnamaldehyde, were reduced to imine compounds to give allylamines(5a-5d) after mrthylation. These compounds which were tested in vitro against five fungal cell lines containing Trichophyton mentagrophytes. showed no activity in 0.1∼100 μg/ml range.

Sclerotinia sclerotiorum(Lib.) de Bary에 의한 들깨 菌劾病과 그 新寄主

曺鐘澤,文炳周 東亞大學校生命資源科學大學附設 農業資源硏究所 1994 農業生命資援硏究 Vol.3 No.2

최근 부산근교의 하우스재배 지대에서는 채소용으로 재배되는 들깨의 줄기에는 잘룩병이 나타나고 잎에는 갈색∼흑갈색의 병반이 형성되어 습하면 연부현상이 나타나며 ???병부에는 菌核이 형성되는 신병해가 발생하여 그 피해가 막심하다. 본 병균의 形態와 病徵을 조사한 결과 Sclerotinia sclerotiorum (Lib.) de Bary로 同定되었는데 그 특징은 다음과 같다. 자낭내의 자낭포자는 그 크기가 동일하고 자낭포자는 2핵이며 자낭포자의 길이와 나비의 비가 2.0보다 크고 자낭반의 皮層은 prismatica조직으로 구성되어 있었다. 菌核은 그 크기가 평균 2.8mm로서 PDA평면배에서 주로 균총 가장자리에 윤상으로 형성되고, 균핵의 皮層은 암갈색 원형의 세포가 2∼6층으로 구성되고 그 내부는 oblita조직으로 구성되며 皮層외부에는 tomentum hypha가 형성되지 않았다. 人工接種에 의하여 본 병원균에 대한 新寄主 10種과 우리나라 미기록 기주 2종을 발견하였다. A new disease of perilla was recently found in the area of house culture near Pusan and it is causing a serious damage of crops in the area. Symptoms of the disease are damping-off of infected stems and also occur on leaves as showing brown to dark brown spots, which is caused a soft rot in wet conditions. Sclerotia of the pathogen is also formed on the infected stem. The pathogen was identified as Sclerotinia sclerotiorum (Lib.) de Bary due to the morphology and symptoms. The detailed characteristics of the pathogen are summarised as follows. 1. Ascospores are equally binucleate and the ratio of length and width is over 2.0. Ectal excipulum at the margin of apothecium is composed of texture prismatica. 2. The size of sclerotia is approximately 2.8mm in diamter and it is usually produced at the growing margin of colony on PDA medium. Cortex of sclerotia is composed of 2-6 layers of dark browned and cycloid cells. It is composed of hyaline texure obilata within sclerotial medulla and not formed tomentum hyphae on outside of cortex. From the artificial inoculation test, 10 plant species were determined as new host plants and 2 plant species unrecorded in Korea were found.

팽이 톱밥 人工栽培時 培地微生物相의 經時的인 密度變化

배태웅,文炳周,辛元敎,宋模烈,李鉉旭,曺東進 東亞大學校生命資源科學大學附設 農業資原硏究所 1996 農業生命資援硏究 Vol.5 No.1

팽이 재배에 있어 가장 큰 문제 중의 하나는 雜菌에 대한 汚染, 특히 細菌에 의한 피해가 극심하므로 作業段階와 栽培過程 전반에 걸쳐 培地徵生物相의 經時的인 密度變化에 따른 雜菌의 培地內 流入時期를 조사하여 汚染菌의 發生消長을 알아보기 위해 現地農家에서 시험해 본 결과 接種후 培養室에서 菌絲培養을 시작한 5일후부터 細菌이 檢出되기 시작하여 菌긁기전까지 약 25일간 0.5∼3.1×10⁴cfu/g의 密度를 보였으나 經時的인 密度增加는 없었고, 菌絲培養이 끝난 뒤 菌긁기직후부터 細菌의 密度는 118.3×10⁴cfu/g으로 급격히 높아졌으며 子實體生育 기간동안에 시간이 경과함에 따라 密度가 현저하게 증가되는 경향을 나타냈다. 이는 培養室 안으로 흐르는 空氣를 통하여 甁內로 細菌이 流入되고 또한 菌긁기 직후 培地表面에 灌注하는 물과 加濕機에서 噴霧되는 물粒子가 細菌의 密度增加에 절대적으로 영향을 미치는 것으로 판단되므로 이때가 細菌性褐斑病菌에 感染되기 가장 용이한 시기로 생각된다. 眞菌은 菌긁기후 子實體生育 기간동안 1.17∼7.87×10⁴/g의 密度로서 菌絲培養 기간동안의 密度보다 다소 높게 檢出되었으나 대체로 經時的인 密度增加는 보이지 않았으며, 放線菌은 거의 검출되지 않거나 菌絲培養 후 5일째부터 다소 검출되기 시작했으나 밀도가 매우 낮았고 經時的으로도 증가되지 않았다. One of the most serious problem on the Enoki mushroom cultivation is the infection by pathogenicitic microorganisns, especially bacterial diseases causing serious economical losses in the mushroom production. It was carried out to find the tendency of the development of mushroom diseases by temporal changes and variation of the densities of media microflora through the cultivation periods and working stages in a mushroom farm. According to the results, several bacteria were detected from the fifth day after the inoculation and incubation of mycelium in the incubation room, and the density of bacterium was 0.5∼3.1×10⁴cfu/g media for 25 days before the inoculum-removing, although there was no increment of the density. Since the inoculum-removing after the spawn run, the density of bacterium was rapidly increased as 118.3×10⁴cfu/g media, and was significantly increased by time during the growth period of fruitbodies. It was hypothesized that it is the most suitable period for the pathogen infection because the bacterium might be introduced by the air in the incubation-room, and the increment of bacterium density might be affected by moisture supplied through watering or humidifier in the growing room after the inoculum-removing. Fungi were detected as the density as 1.17∼7.87×10⁴/g media and the density was slightly higher than that during the incubation period of hyphae, although there was no significant increment. Generally, the density of Actinomycetes was disregardfully low, or Actinomycetes were hardly ever found, although there was found some 5 days after incubation hyphae in few cases.